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Adult New-Zealand rabbits were vaccinated subcutaneously with three doses of 100 µg of immunocomplexes formed by serum immunoglobulins of rabbits vaccinated with infective larvae somatic extracts (L3SE) and adult nematode phosphate buffered saline- soluble proteins (ASE, group II) or L3SE (group I). Injections of the immunogens were accompanied with beryllium hydroxide, Be(OH)₂ as an adjuvant. Vaccinated rabbits and also those of group III were infected orally with 10,000 infective larvae of T. colubriformis 14 days after antigen injection and necropsied in groups of four on day 4, 8 and 21 after challenge (DACh). Worm burdens found in vaccinated rabbits were significantly lower than in group III only on day 4 (groups I and II) and 8 (group II) after challenge. The degree of protection oscillated at that time between 47-59%. Mesenteric lymph node leukocyte responses were measured using a leukocyte migration inhibition assay. The most significant MLN leukocyte response occurred in all groups on 21 DACh. However, in group II a marked leukocyte reaction started already on 4 DACh. In ELISA tests IgA antibodies specific to ASE reached the highest level in the bile and serum of group II. The mucosal IgG response was the highest in group I until 8 DACh but in group II and III on 21 DACh.
The target of the study was to examine histochemically in vivo the activity of prostaglandin synthetase in Trichinella spiralis larvae in stages of their migration, settlement and encapsulation in the host’s tissues. It was proved that prostaglandins may play certain role in producing circumlarval capsules.
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Ocular cysticercosis with intermittent blindness

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We report this peculiar case of ocular cysticercosis with intermittent blindness which is never reported to our knowledge. This case highlights use of Ultrasound for examination of the posterior segment of eye globe. In limited facilities Ultrasound is the main modality to reach the diagnosis. We also wish to enforce the point that in endemic regions and in young population primary cysticercosis and its complications must be kept in differentials of cystic mass or calcified mass, as in this case, we have seen both the stages of cysticercosis progression.
Larvae and pupae of Macropeza albitarsis MEIGEN, 1818 are described and illustrated for the first time. Aquatic larvae with a paddle-like anal segment armed with dorsal and ventral broom hairs are good swimmers. Within the family, larvae of the genus Macropeza MEIGEN, 1818 are unique in having broom hairs on the anal segment and a pair of multiple setae p on the head.
The aim of the present study was to verify the feasibility of using live Artemia salina nauplii embedded with fluorochromes for the mass marking of pike Esox lucius (L.) larvae. In the experiment, pike larvae 6 days post hatch were fed ad libitum with nauplii dyed with 600 ppm tetracycline hydrochloride (TC) or 200 ppm alizarin red S (ARS) for 3 or 6 days. The highest percentage of marked fish (100%) and the best quality of this marking was found in the groups fed A. salina stained with TC for either 3 or 6 days. In groups fed A. salina stained with ARS for 3 or 6 days exhibited a lower percentage of marked fish (ranging from 76.7–88.3%). No significant differences between experimental groups were noted regarding survival rate, final body weight and length of the reared pike larvae.
The development of the nase liver was examined under light and electron microscopes from the moment of hatching until the juvenile stage. Three phases of hepatocyte differentiation were observed during the organogenesis of nase livers. In the first phase, from hatching until day 4, the hepatoblasts of the primordial liver are morphologically undifferentiated and divided by sinus vessels. They also store glycogen. In the second phase, from the moment when the mouth cavity becomes passable until the resorption of the yolk sac, organelles typical of the structure of hepatocytes appear and begin to function. At the end of this phase signs of bile lipid synthesis and secretion become visible. The third phase is when exogenous nutrition begins and is characterized by the increased activity of the significant organelles engaged in protein synthesis and secretion, such as the endoplasmic reticulum and the Golgi apparatus.
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