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The pine processionary moth, Taumetopoea pityocampa (Denis and Schiffermüller) is one of the most widespread defoliator insects found in the forest areas of Turkey. Although different methods have been used to control this major forest pest up to now, the problem is still going on largely unsolved in the forest areas of Turkey. The objective of this study was to determine larvicidal effects of extracts obtained from six different plant species, Achillea wilhelmsii C. Koch, Nepeta meyeri Benth., Satureja hortensis L., Origanum onites L., O. rotundifolium Boiss., Tanacetum argyrophyllum (C. Koch) and on the 2nd, 3rd, and 4th instar larvae of T. pityocampa in laboratory conditions.Test the toxicity of six plant extracts against to the 2nd, 3rd, and 4th instar larvae of T. pityocampa, 10 larvae of this insect with 15 gr amounts fresh needles (1 year old) of Pinus brutia were placed to Petri dishes (9 × 1.5 cm deep). Each dose was dissolved in acetone and 0.25, 0.5 and 1 mg of the plant extracts found in 1 ml solution were sprayed on the 2nd, 3rd, and 4th instar larvae of T. pityocampa in the Petri dishes, corresponding to 2.08, 4.16 and 8.33 mg·l -1 air concentrations. Petri dishes were covered with a lid. All tests carried out at 26ºC (±2), 60% (±5) relative humidity and 14/10 h light/dark photoperiod in laboratory conditions. When exposure, mortality of the larvae was after the determined at 24, 48 and 96 h. Petri dish applied with sterile water and acetone were used as control group. All the tests were made in triplicate. The results showed that six plant exracts have a larvicidal effect on the 2nd, 3rd, and 4th instar larvae of T. pityocampa in comparison with controls. Therefore, these naturally occurring plant extracts could be useful for managing the larvae populations of T. pityocampa.
The paper presents the results of laboratory research on the effects of different soil compaction caused by varying unit pressure and its impact on germination and growth parameters of common beech. Soil for the research was taken from the forest stand, and then poured into prepared PVC cylinders. Pressure was exerted on the soil with use of the stamp, on a specially prepared static stand. Unit pressure ranged from 50 to 300 kPa with 50 kPa resolution. Each variant of the exerted unit pressure and control without pressure was repeated three times. 21 seeds of common beach were sown into a single cylinder. The cylinders were then placed in controlled conditions and after 2.5 months all seedlings parameters were measured. 234 seedlings were analysed (sown 441). It was found that the seeds were influenced by the place of sowing. There were significant correlations between the exerted unit pressure and the length of root system of plants sown away from direct exposure to the stamp, as well as all the plants together. With the increased unit pressure the length of root system decreased. Sowing place turned out to be important for the root neck diameter, root dry weight and whole plants.
Insecticides are unavoidable in pest management programs especially when the pest crosses Econimic Threshold Level (ETL). Nevertheless, often the plant protection products kill the natural enemy population making the pest to resurge and thus demanding more sprays. Therefore, insecticides used in IPM programs should be selective enough to spare the beneficials. Laboratory studies were conducted to find out the toxicity of imidacloprid and diafenthiuron to the eggs, larvae and adults of Chrysoperla carnea. Imidacloprid at the recommended dose of 0.28 ml/l caused 15.38% egg mortality, 26.67 and 33.33% larval mortality by ingestion and contact, respectively and 50.00% adult mortality. The egg mortality was about 15.38% and larval mortality of 23.33% and adult mortality of 26.67% was caused by diafenthiuron. Based on the classification given by IOBC/WPRS working group on Pesticides and non-target invertebrates, both the insecticides were classified as harmless to C. carnea, since the recommended dose caused less than 50% mortality in the laboratory conditions.
Interactions between 42 higher fungi and six strains of Heterobasidion annosum (Fr.) Bref., representing P and S intersterility groups were studied in vitro. Part of the higher fungi was represented by strains of ectomycorrhizal fungi indigenous to old disease centres caused by H. annosum in pine stands. Variation in antagonism to the pathogen was observed both within and between the species. The antagonism was dependent on growth rates of fungi in control. Fungi growing faster displayed a greater ability to arrest the development of the pathogen mycelia. The same tendency was observed in the strains of H. annosum, which generally grew faster and their growth was restricted less than that of higher fungi. Two strains of an ectedomycorrhizal fungus Mrg X, accompanied by a helper bacteria reduced mycelial extension of H. annosum by 72% and 76%. Among ectomycorrhizal fungi, the most effective antagonists were strains of Xerocomus subtomentosus, Amanita muscaria, A. citrina and Laccaria laccata. Three various types of interaction between the studied fungi were observed. Inhibition zone between interacting mycelia appeared often, however the fungi displayed this antagonistic property not to all strains of the pathogen. Although mycelia of H. annosum invaded colonies of the other fungi frequently, in none of the treatments growth of the higher fungus over the opposing colony of the pathogen was observed.
Balea fallax (Rossm.) collected from the Roztocze Upland (SE. Poland) was kept in the laboratory for four years. Observations were conducted between March and October when the snails were kept at room temperature (18-25°C); in winter they were stored at 3°C. The egg-laying period started in late March and lasted till October, with maxima in spring and early autumn. The snails laid oval, gelatinous eggs with separatecalcium carbonate crystals in the external envelope (average egg size 1.96 × 1.73 mm). The eggs were deposited in batches (up to 14 eggs at a time) or singly. The number of batches per snail per year ranged between 1and 4. Snails isolated before maturation laid defective eggs which failed to develop, which suggest that the speciesis incapable of uniparental reproduction or at least the ability is very limited. The reproduction rate decreased during consecutive years, probably as a result of the aging or the shortage of allosperm in isolated individuals.Compared to batches of typically oviparous clausiliids, eggs of B. fallax hatched slightly earlier (interval between oviposition and hatching lasted 8-10 days at room temperature). It is likely that the adults retained developing eggs in the uterus for a short time. The juveniles needed at least 6 months to attain the ultimates hell size.
The efficacy of UV treatment to control bacterial adhesion onto hard surfaces was investigated in laboratory conditions. The major characteristics necessary for biofilm formation like extracellular polymeric substance (EPS) production, carbohydrate and protein concentration in EPS, and adhesion ability onto hard surface were studied using two bacterial strains isolated from marine biofilms. The results showed that there was a considerable difference between the control and UV treated bacterial cultures in their viability, production of EPS, and adhesion ability. The protein and carbohydrate concentration of the EPS and the adhesion of bacterial cells to surface were also considerably reduced due to UV treatment. This study indicates that treatment of water with UV light may be used to control biofilm development on hard surfaces.
The aim of the research was to compare alluvial bed topography description in laboratory conditions using the “traditional”, currently applicable method with an original approach, based on LiDAR technology. LiDAR application in local scours shape investigation in based on the grounds of introducing the autonomic measuring module, which, placed above the bed on dedicated controllable arrangement of guideways, describes the landform as a cloud of coordinates. The result of the performed experiment was obtainment of point clouds (x, y, z), reflecting the bed shape before and after local scour formation during twenty measurement series with varying hydraulic conditions. Objects of the study were basic geometry properties of the scour hole and its volume. The measurement with laser scanner technology application allowed for obtaining much more accurate results in shorter time, comparison to disc probe survey, and also relatively fast conversion of numerical data into figures. The device equipped with portable computer, precise stepper motors and dedicated software permitted the introduction of automation into laboratory work. The effect is not only measurements accuracy, but also significant acceleration of data gathering. The adopted grid is characterized by significant density, which – in connection with meaningfully high accuracy – allows very precise surface description. Bed shape can be presented in numerical or graphical form. It must be pointed out that disc probe method application would never give such accuracy as in the case of introducing laser scanning technology in similar studies.
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