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The failure of the therapy of fractures in the form of a delayed union of the fractured bone or lack of this union, gave rise to research on the best conditions that are essential for a successful regeneration of the bone. The aim of this study was to present a semi-invasive stimulation method in vivo. The femur in 20 rabbits was sawed, and then stabilized by the Mikro-Zespol method. Electrostimulation was not used in the control group. Pulsed unidirectional electrical current of 70 micro A, 25% filled and 1 Hz (mean value 17.5 micro A) was applied in the experimental group to stimulate the fracture area. The animals were clinically and radiologically assessed. Bones of the sacrificed animals were sampled for histological examination. The bone repair process was more advanced in the experimental group in comparison to the control group in early research periods. Any pathologic findings related to cytotoxicity and carcinogenesis were not observed in livers and kidneys of the animals. This is an animal study and further clinical trials are needed to determine whether semi-invasive electrical stimulation method will be able to reduce the rate of bone healing disturbances.
The aim of the present study was to determine the effect of the manner of housing on the growth and mechanical strength of the femur and tibia in heavy (Big 6) and medium-heavy type (BUT 9) turkey-toms. The birds were raised from 7 to 22-weeks-of-age indoors - in a brooder house (traditional system) or under a shelter with access to open-air runs (alternative system). The housing system significantly affected the results of a strength test of femur and tibia. The femurs of turkeys that stayed under a shelter with access to outdoor runs were characterized by higher values of strain, capacity to absorb strength and work to the limit of elasticity and to the limit of maximum resistance. This housing system also had a positive effect regarding the weight of the femur and tibia (increased by 5.9%). Bone strain, capacity to absorb work to the limit of maximum resistance as well as the capacity to absorb strength to the limit of elasticity and to the limit of maximum resistance were affected by the type of turkeys. The weight of the tested bones was higher (by 6.7%) in Big 6 toms. The manner of housing (shelter with access to open-air runs) significantly influenced tibia strain and the capacity to absorb work to the limit of maximum resistance. The change in the housing system was reflected in the values of Young’s modulus and bone weight. Significant differences were also found among the types of turkeys in the capacity to absorb strength to the limit of elasticity and to the limit of maximum resistance as well as in bone weight. The results of femur and tibia strength testing indicate that it is recommended to raise slaughter turkey-toms under a shelter with access to outdoor runs starting from 7-weeks-of-age.
The purpose of the study was to determine the effect of Strontium Ranelate (RS) on the mineral density and mechanical properties of the femur in male Wistar rats during experimental osteopenia induced by bilateral gonadectomy. After adaptation, the rats were randomly divided and subjected to sham-operations (SHO) (n = 10) and bilateral orchidectomy (ORX) (n = 20). ORX rats were subsequently divided into the control group (ORX/K) and experimental group treated with RS (ORX/RS), administered by gavage, at a dose of 900 mg/kg b.w. The experiment lasted 60 days and then the animals were killed, blood collected, and femora isolated. Femora were analyzed using peripheral quantitative computed tomography (pQCT) to determine volumetric bone mineral density of the cortical part of the shaft of the column (Ct.vBMD), endoosteal and periosteal circumferences, cortical thickness (Ct.Th) and Strength Strain Index (SSI). Measurements were performed on 50% of bone length. The bones were tested using the three-point bending test to estimate ultimate strength and resilience. The activity of alkaline phosphatase (ALP) and concentrations of Ca and P in the blood serum were estimated. The obtained results indicated that the use of strontium ranelate in orchidectomized rats over a period of 60 days not only inhibited the reabsorbing processes induced by a lack of gonad steroids but also accelerated processes of new bone tissue formation, which improved their mechanical parameters and ultimate strength and maximum elasticity.
Zbadano zawartość magnezu w pobranych śródoperacyjnie próbkach tkanki zbitej szyjki i gąbczastej głowy kości udowych pochodzących od 24 pacjentów, u których wykonano endoprote- zoplastykę z powodu patologicznego lub urazowego złamania szyjki kości udowej. U chorych ze złamaniem patologicznym, po porównaniu z grupą kontrolną, w obrębie szyjki kości udowej stwierdzono statystycznie znamienne obniżenie gęstości masy kostnej. Natomiast parametry biochemiczne: stężenie magnezu i stosunek Mg/Ca w obu badanych grupach nie różniły się znamiennie. Na podstawie otrzymanych wyników wyciągnięto następujące wnioski: (1) zawartość Mg w strukturach: zbitej i gąbczastej szyjki kości udowej jest w obu badanych grupach zbliżona. (2) zaobserwowana w grupie badanej nieznacznie mniejsza zawartość Mg w części korowej może pozostawać w związku ze złamaniem samoistnym.
This paper focuses on analysing the usefulness of micro-CT scans in assessing the efficacy of hydroxyapatite tricalcium phosphate in the treatment of experimental femoral bone defects in rabbits. The tests were conducted on 12 male Termond White rabbits, which had previously undergone bilateral surgical interventions involving femoral bone diaphysis (shaft) performed under operating-theatre conditions. The bones were drilled with a 4 mm drill. All rabbits had their left femur defects left without filling, whereas the right femur defects were filled with 100 mg of hydroxyapatite tricalcium phosphate preparation. After 8 weeks, the animals were euthanized to take specimens of both femurs. Micro-CT scans were taken with a Skyscan 1174 (Bruker – SkyScan). The area examined was subjected to volume rendering to determine the following parameters: Vb [mm³] – total volume of the specimen, Vb₁ [mm³] – high-density fraction volume in the specimen, and Vb₂ [mm³] – low-density fraction volume in the specimen, calculated by the formula Vb₂ [mm³] = Vb – Vb₁. Subsequently, after appropriate preparation, the specimens were assessed histopathologically. The analysis of micro-CT scan results showed a statistically significant increase in the total volume (Vb) of the part of the femur filled with the bone substitute (B) compared to the control group (A), and an increase in the low-density fraction volume (Vb₂). The volume of high-density fraction tissue (Vb₁) showed no statistically significant difference between the groups. The histopathological assessment of the control-group specimens (A) showed that bone defects had been completely filled by osteoid tissue with an irregular, feathery structure, and the cortical layer exhibited a marrow-induced thickening. The specimens from group (B) showed amorphous masses of the bone-substitute preparation surrounded by callus formed concentrically around preparation particles.
Oznaczono zawartość glinu oraz podstawowych biopierwiastków Ca, Mg, Zn, Cu, Fe w kości udowej i wątrobie zwierząt doświadczalnych, u których wywoływano kumulację glinu w tkankach, a następnie poddano działaniu deferoksaminy. Poszukiwano zależności między stężeniem glinu a poziomem biopierwiastków w badanych tkankach oraz zmiany wywołane działaniem deferoksaminy - chelatora glinu.
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