Wyniki wyszukiwania - AGRO

1

A simple procedure for isolation of microsomes from human placenta

100%

Gniot-Szulzycka J., Januszewska B.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 3

2

Antibiotic resistance of bacteria of the family Enterobacteriaceae isolated from vegetables - short report

100%

Laniewska-Trokenheim L., Sobota M., Warminska-Radyko I.

Polish Journal of Food and Nutrition Sciences

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2006

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tom 15/56

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nr 4

Antibiotic resistance of 114 strains of the Enterobacteriaceae family bacteria isolated from vegetables, originating from retail, was investigated in the study. The highest number of the strains isolated were resistant to ampicilin (81.9%), whereas a lower number of the strains exhibited resistance to the following antibiotics: neomycin (29.3%), streptomycin (28.4%), rifampicin (21.5%), chloramphenicol (19.8%), colistin (12.9%), and nitrofurantoin (11.%). All the isolated strains appeared to be susceptible to vancomycin, kanamycin, doxycyclin, nalidixic acid and gentamycin.

3

Isolation and properties of the subunit form EF-1C of elongation factor 1 from Guerin epithelioma cells

100%

Marcinkiewicz C., Galasinski W.

Acta Biochimica Polonica

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1993

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tom 40

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nr 2

EF-1C is a component of the aggregate EF-1B, consisting of the subunit forms EF-1A-EF-1C; it was isolated by dissociation of this aggregate in the presence of GTF. The subunit form EF-1C stimulates binding of aminoacyl-tRNA to ribosomes, catalysed by EF-1A, similarly as EF-lßy which stimulates the activity of EF-1 in other eukaryotic cells. EF-1C in the presence of 6 M urea was separated into two polypeptides. Polypeptide of molecular mass 32000 Da is responsible for regeneration of the EF-1A-GTP active complex. Thermal sensitivity of EF-1A was much higher than that of EF-1B, thus a protective role of EF-1C in the EF-1AEF-1C complex is suggested.

4

Identification and antibacterial resistance of bacteria isolated from poultry

88%

El-Rami F. E., Sleiman F. T., Abdelnoor A. M.

Polish Journal of Microbiology

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2012

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tom 61

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nr 4

Food-borne infections are among the prominent health hazards. Antibacterial agents (ABA) are usually administered to poultry in Lebanon as antibiotic growth promoters (AGP), which might lead to the dissemination of resistant bacterial strains. e aims of this study were to isolate potential food borne pathogens from poultry and investigate an association between AGP usage and antibacterial resistance (ABR). Isolates were obtained from the culture of cloacae swabs and identified. Escherichia coli was the predominant isolate. There was a significant association between the use of tetracycline and gentamicin as AGP and the number of E. coli isolates resistant to these ABA.

5

Antibiotic susceptibility and biofilm-forming capacity of enterococci isolated from food of animal origin

88%

Duckova V., Canigova M., Krocko M., Lavova M.

Medycyna Weterynaryjna

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2014

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tom 70

|

nr 01

Enterococci are found in the gastrointestinal tract of humans and animals, in soil, and in water, but they also have a long history of use in the production of traditional fermented food. Some strains of enterococci are considered as emerging pathogens of humans. The intrinsic and acquired resistance of enterococci to antibiotics has special significance. Food of animal origin may be a source of resistant enterococci. The main cause of food contamination with enterococci is improper cleaning and disinfection of equipment. The aim of this study was to assess the antibiotic susceptibility of enterococci isolated from different types of food of animal origin and the ability of these microorganisms to form biofilm. Out of a total of 63 isolates, relatively few enterococci were resistant to gentamicin (1.59%). The highest level of resistance was noted for vancomycin (7.94%) and ampicillin (9.52%). However as many as 19.05% and 44.44% of enterococci were resistant to erythromycin and tetracycline, respectively. The ability to form biofilm was detected in 14.29% of the strains of enterococci tested.

6

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Evaluation cephalosporins resistance in pathogenic bacteria isolated clinically

88%

AL-Khikani F. H. O., Kadim B. J., Ayit A. S., Abidalali M. H.

World News of Natural Sciences

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2020

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tom 31

7

Isolation and culture of protoplasts within the genus Beta

88%

Niklas A., Jazdzewska E., Majewska-Sawka A.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

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1997

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tom 39

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nr 1

8

Effects of social rearing conditions on conditioned suppression in rats

88%

Walasek G., Wesierska M., Werka T.

Acta Neurobiologiae Experimentalis

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2002

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tom 62

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nr 1

9

Isolation and in vitro culture of ovarian preantral and early antral follicles in cattle

88%

Katska L., Rynska B.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

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nr 2

10

Investigation of conditions of protoplasts isolation from Asparagus and Hyacinthus

88%

Pindel A., Lech M., Bach A.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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1999

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tom 41

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nr 1

11

Antiviral and cytotoxic activities of anthraquinones isolated from Cassia roxburghii Linn. leaves

75%

Mohammed M. M. D., El-Souda S. S., El-Hallouty S. M., Kobayashi N.

Herba Polonica

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2013

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tom 59

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nr 4

The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. against HCT-116 and MCF-7 cell lines resulted with IC50=34.9 and 38.04 μg/ml, respectively, while against HepG-2 showed no activity. A bioassay guided-fractionation approach was conducted to isolate and identify the active cytotoxic principles. Further chromatographic separation and purification of the petroleum ether extract resulted in the isolation of two anthraquinones identified as aloe-emodin acetate and aloe-emodin, along with stigmasterol, β-sitosterol and palmitic acid. The structure elucidation of isolated compounds was performend using 1D, 2D-NMR and HR-MS. Furthermore, the cytotoxicity of aloe-emodin acetate and aloe-emodin were evaluated and resulted with IC50=153.30 and 70.02 μg/ml against HCT-116 and with 93.20 and 53.20 μg/ml against MCF-7, respectively, while against HepG-2 showed no activity. Moreover, the antiviral activity of the two isolated anthraquinones was tested against influenza virus-A, and resulted with IC50=10.23 as well as 2.00 and with CC50=1.32 and 0.47 μg/ml, respectively.

12

Isolation of outer membrane proteins [OMP] from Salmonella cells using zwitterionic detergent and their separation by two-dimensional electrophoresis [2-DE]

75%

Futoma-Koloch B., Bugla-Ploskonska G., Doroszkiewicz W.

Polish Journal of Microbiology

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2009

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tom 58

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nr 4

363-366

We report that using the zwitterionic detergent Zwittergent Z 3-14® to isolate outer membrane proteins (OMPs) from Salmonella O48 is suitable for their separation by two-dimensional electrophoresis (2-DE) in a capillary tube system. Sample preparation is a very crucial step for any bacterial proteomic study. Some modifications were introduced to the 2-DE protocol suggested by O'Farrell and BioRad, which significantly impaired the resolution of proteins. 2-DE analysis of OMPs may be helpful in the interpretation of the variable susceptibility of Salmonella O48 rods to the bactericidal activity of serum.

13

Use of selected metal ions for the separation of peptides isolated from thermally processed string beans

75%

Karas M., Baraniak B.

Journal of Elementology

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2010

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tom 15

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nr 2

291-300

Recent years have witnessed growing interest in research on the structure and properties of proteins and peptides as physiologically active dietary components. The above has spurred a new interest in the isolation of animal, plant and microbiological peptides and investigation of their biological activity. The isolation and separation of protein and peptide mixture is not an easy procedure. Immobilised Metal Ion Affinity Chromatography (IMAC) is increasingly often used in this process. Affinity chromatography relies on the specific interactions between amino acids, their reactive groups in peptides and metal ions. The objective of this study was to determine whether copper and nickel ions can be used for the separation of peptides isolated from string beans than had been blanched and heated in a microwave oven. In this study, peptides extracted with 1% trichloroacetic acid (TCA) from string beans that had been blanched and heated in a microwave oven, were separated by chromatography on columns with copper and nickel ions immobilised through iminodiacetic acid (IDA). Peptide concentrations of the separated fractions were determined. Peptides found in string beans had similar affinity for metal ions in the Cu > Ni sequence, with selectivity in the Ni > Cu sequence. Microwave heating of string beans decreases the peptide content of extracts isolated with 1% TCA. The resulting changes are dependent on the duration of the process and the type of heating medium. Affinity chromatography with the use of metal ions immobilized to iminodiacetic acid (IDA)-Sephadex G-25 may be successfully used for the separation of peptides isolated from string beans.

14

The activity of chosen bacteriophages on Yersinia enterocolitica strains

75%

Kot B., Bukowski K., Jakubczak A., Kaczorek I.

Polish Journal of Veterinary Sciences

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2002

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tom 05

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nr 1

15

Genetic comparison of Campylobacter jejuni isolated from different cattle farms

75%

Bednarski M., Wieliczko A., Mazurkiewicz M.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 2

To compare the genotypes of Campylobacter jejuni, isolates of cattle origin were collected from 9 Polish farms and genotyped by ERIC-PCR. We identified 28 genotypes among the 43 C. jejuni isolates, and demonstrated high genomic diversity. The highest level of diversity was observed in strains isolated from stanchion-barn animals in opposition to those from the loose-housing system.

16

Molecular identification of polyhydroxyalkanoates-producing bacteria isolated from enriched microbial community

75%

Ciesielski S., Pokoj T., Mozejko J., Klimiuk E.

Polish Journal of Microbiology

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2013

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tom 62

|

nr 1

Polyhydroxyalkanoates (PHAs) are especially interesting because of their similar properties to synthetic plastics and their potential use as biodegradable polymers. Many strategies have been employed to effectively and economically produce PHAs, among them a production process based on mixed microbial populations, enriched from activated sludge could be one of the alternative technologies. Defining the bacterial species creating these anonymous populations is crucial for the improvement of cultivation strategy. Moreover, enriched bacterial populations could be a promising source for microbes, useful in many biotechnological projects. The main object of this study was to characterize the microorganisms creating the microbial consortium cultured towards PHAs production. After cultivation, bacteria were identified using the 16S rRNA gene sequencing approach. The presence of genes engaged in PHAs synthesis was detected using PCR. The performed analysis revealed that among eleven isolated bacterial strains, four possessed the ability of polyhydroxybutyrate synthesis.

17

Susceptibility of yeasts isolated from dairy products to cystatin

75%

Chrzanowska J., Krajewska E., Saleh J., Szoltysik M., Trziszka T.

Polish Journal of Natural Sciences. Supplement

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2004

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tom 02

23-29

18

Yeast-like fungi isolated in students

75%

Biedunkiewicz A.

Acta Mycologica

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2007

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tom 42

|

nr 1

The occurrence of yeast-like fungi in the most important infection portals of the respiratory system in 200 randomly chosen students of biology and veterinary medicine was examined. The students come into direct contact with plants and animals that may be colonised by fungi belonging to various systematic groups. nine species of yeast-like fungi, including 7 species determined in the biologists, were recorded in the subjects. Candida tropicalisand C.albicanswere the most frequently isolated fungi. The greatest number of fungi was isolated from the oral cavity (124 isolates), fewer from the throat (79 isolates), and the smallest number from the nose (8 isolates). Fungi occurred more frequently in autumn and slightly less frequently in spring, and were isolated more frequently from women than from men.

19

Application of Saccharomyces cerevisiae and Pichia stipitis karyoductants to the production of ethanol from xylose

75%

Kordowska-Wiater M., Targonski Z.

Acta Microbiologica Polonica

|

2001

|

tom 50

|

nr 3-4

291-299

20

The study of canine atopic dermatitis involving the isolation of dogs

75%

Fujimura M.

Polish Journal of Veterinary Sciences

|

2011

|

tom 14

|

nr 2

Twenty-seven pruritic dogs were used in this study. When a hypoallergenic diet was fed to these 27 dogs for six weeks, none of the dogs showed improvement of the pruritus. These dogs had a history and clinical signs of atopic dermatitis (AD) as defined by Prelaud’s diagnostic criteria. Subsequently, the 27 dogs were isolated for observation for two weeks in the hospital. In the isolation room in the veterinary clinic, cages and tableware were all stainless steel, and carpet was not used. A hypoallergenic diet was continuously fed to the 27 dogs for two weeks, during which time they were kept in the isolation room. PVAS (Pruritus Visual Analog Scale) was performed prior to starting the isolation, at the start of the study and 2 weeks after starting the isolation. In 17dogs (63%) the pruritus improved in the isolation room. A statistically significant reduction (p<0.01) of PLS (Pruritus liners score) was recorded 2 weeks after isolation. It was hypothesized that the 17 dogs whose pruritus improved in the isolation room had AD caused by an environmental antigen that was not present in the isolation room. Pruritus of the remaining 10 dogs (37%) did not improve. For 6/10 dogs, the intradermal allergy testing was positive for an environmental antigen. For4/10 dogs, the intradermal allergy testing was negative for all environmental antigens. Dogs for which sensitivity to an environmental antigen was not identified were thought to have atopic-like dermatitis.

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