Wyniki wyszukiwania

1

The calcium sensing receptor modulates H+-ATPase activity in intercalated cells

100%

Gomes Coutinho A. G., Pinheiro E., Fernandez R.

Journal of Physiology and Pharmacology

|

2020

|

tom 71

|

nr 6

2

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

The role of intracellular calcium in the ischemic myocardial damage

100%

Lewartowski B., Pytkowski B., Janczewski A. M.

Acta Physiologica Polonica

|

1987

|

tom 38

|

nr 6

3

Adenosine 5'-triphosphate - a new regulator of annexin function. A hypothesis

100%

Bandorowicz-Pikula J., Pikula S.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 3

The paradigm of annexins as phospholipid-binding proteins interacting with membranes in a calcium-dependent manner has been recently questioned in light of observations that some annexin isoforms may behave like membrane integral proteins or remain associated with their target membranes at low, resting, concentrations of Ca2+ in the cytoplasm. In addition, an evidence has been presented that some annexins (annexins I, VI and VII) bind in vitro ATP and GTP, and upon binding the nucleotide the in vitro activity of these proteins is modified. However, annexins do not contain Walker A and B consensus sequences for ATP/GTP binding. This review presents the hypothesis that a new ATP-binding motif exists within the annexin molecules and that ATP may play a role of functional ligand for annexins also in vivo

4

Molecular dissection of fertilisation signalling with the aid of tyrosine-kinase-specific inhibitors

100%

Sato K. I., Iwasaki T., Fukami Y.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 2A

5

Temperature can prime human peripheral blood neutrophils in a p38MAPKalpha-dependent manner

100%

Jozefowicz-Okonkwo G., Nowak D.

Archivum Immunologiae et Therapiae Experimentalis

|

2004

|

tom 52

|

nr 6

6

Crataegus special extract WS 1442: up-to-date review of experimental and clinical experiences

84%

Zorniak M., Szydlo B., Krzeminski T. F.

Journal of Physiology and Pharmacology

|

2017

|

tom 68

|

nr 4

7

The effect of progesterone on oxytocin-stimulated intracellular Ca2plus mobilisation and prostaglandin secretion in porcine endometrium

84%

Kotwica G., Oponowicz A., Kurowicka B., Franczak A., Bogacka I.

Polish Journal of Veterinary Sciences

|

2010

|

tom 13

|

nr 4

We have studied in the porcine endometrium the expression of oxytocin receptor (OTR) mRNA and the effect of progesterone (P₄) on oxytocin/oxytocin receptor (OT/OTR) function concerning intracellular Ca²⁺mobilisation ([Ca²⁺]i), prostaglandin F2α (PGF2α) and E2 (PGE2; PG) secretion. Tissue was taken from cyclic and early pregnant pigs (days 14-16). A higher expression of OTR mRNA (P<0.05) was observed in the endometrium of cyclic than pregnant pigs. The stimulatory (P<0.05) effect of OT (10⁻⁷ M) on [Ca²⁺]i mobilisation was noticed within 15-60 s and 30-60 s in endometrial stromal cells of cyclic and pregnant pigs, respectively. In the presence of P₄ (10⁻⁵ M) basal and OT-stimulated [Ca²⁺]i concentrations decreased in stromal cells during luteolysis and pregnancy. In stromal cells P₄ delayed mobilisation of [Ca²⁺]i in response to OT by 15 s during luteolysis and had no effect during pregnancy. In cyclic and pregnant epithelial cells OT stimulated mobilisation of [Ca²⁺]i in 45 s and 60 s, respectively. Oxytocin increased (P<0.05) PGF2α secretion during luteolysis and pregnancy and PGE2 during luteolysis from endometrial slices. Progesterone did not inhibit this stimulatory effect. During luteolysis OT increased (P<0.05) PGF2α in epithelial and stromal cells and PGE2 secretion in epithelial cells. In the presence of P₄ this effect of OT was reduced only in stromal cyclic cells (6 h culture). The presence of P₄ decreased the effect of OT on [Ca²⁺]i mobilisation only in stromal cells. We found that, in most conditions, P₄ did not inhibit the OT-stimulated secretion of PG in the porcine endometrium.

8

Free intracellular calcium [(CA2plus)i] in opioid sensitive cells of the porcine anterior pituitary

84%

Szafranska B., Tilton J. E.

Journal of Physiology and Pharmacology

|

2000

|

tom 51

|

nr 3

9

Effect of Ca2 plus and La3 plus on the callus differentiation in wheat

84%

Filek M., Marcinska I., Biesaga-Koscielniak J.

Biological Bulletin of Poznań. Supplement

|

1997

|

tom 34

10

Mobilization of intracellular calcium by intracellular flash photolysis of caged dihydrosphingosine in cultured neonatal rat sensory neurones

84%

Ayar A., Thatcher N. M., Zehavi U., Trentham D. R., Scott R. H.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

The ability of dihydrosphingosine to release Ca2+ from intracellular stores in neurones was investigated by combining the whole cell patch clamp technique with intracellular flash photolysis of caged, N-(2-nitrobenzyl)dihydrosphingosine. The caged dihydrosphingosine (100 μM) was applied to the intracellular environment via the CsCl-based patch pipette solution which also contained 0.3% dimethylformamide and 2 μM dithiothreitol. Cultured dorsal root ganglion neurones from neonatal rats were voltage clamped at -90 mV and inward whole cell Ca2+-activated currents were recorded in response to intracellular photorelease of dihydrosphingosine. Intracellular photorelease of dihydrosphingosine (about 5 μM) was achieved using a Xenon flash lamp. Inward Ca2+-activated currents were evoked in 50 out of 57 neurones, the mean delay to current activation following photolysis was 82±13 s. The responses were variable with neurones showing transient, oscillating or sustained inward currents. High voltage-activated Ca2+ currents evoked by 100 ms voltage step commands to 0 mV were not attenuated by photorelease of dihydrosphingosine. Controls showed that alone a flash from the Xenon lamp did not activate currents, and that the unphotolysed caged dihydrosphingosine, and intracellular photolysis of 2-(2-nitrobenzylamino) propanediol also did not evoke responses. The dihydrosphingosine current had a reversal potential of -11±3 mV (n = 11), and was carried by two distinct Cl- and cation currents which were reduced by 85% and about 20% following replacement of monovalent cations with N-methyl-D-glucamine or application of the Cl- channel blocker niflumic acid (10 μM) respectively. The responses to photoreleased dihydrosphingosine were inhibited by intracellular application of 20 μM EGTA, 10 μM ryanodine or extracellular application of 10 μM dantrolene, but persisted when Ca2+ free saline was applied to the extracellular environment. Intracellular application of uncaged dihydrosphingosine evoked responses which were attenuated by photolysis of the caged Ca2+ chelator Diazo-2. Experiments also suggested that extracellular application of dihydrosphingosine can activate membrane conductances. We conclude that dihydrosphingosine directly or indirectly mobilises Ca2+ from ryanodine-sensitive intracellular stores in cultured sensory neurones.

11

Hydrogen peroxide in expired air condensate correlates positively with early steps of peripheral neutrophil activation in asthmatic patients

84%

Antczak A., Nowak D., Bialasiewicz P., Kasielski M.

Archivum Immunologiae et Therapiae Experimentalis

|

1999

|

tom 47

|

nr 2

12

Screening of chromosomal region 21q22.3 for mutations in genes associated with neuronal Ca2plus signalling in bipolar affective disorder

84%

Kostyrko A., Hauser J., Rybakowski J. K., Trzeciak W. H.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 2

The therapeutic effect of lithium in bipolar affective disorder may be connected with decreasing intracellular Ca2+ concentrations. Several linkage studies have identified a potential bipolar affective disorder susceptibility locus within chromosomal region 21q22.3. This locus contains two genes expressed in the brain - ADARB1 and TRPM2 - involved in regulating intracellular Ca2+ concentrations. The aim of this study was an identification of mutations in the coding sequences of ADARB1 and TRPM2 and their association with bipolar affective disorder. For that purpose we screened 60 patients with bipolar affective disorder and a control group of 66 subjects using single strand conformation polymorphism and sequence analysis. For rapid screening we performed restriction fragment length polymorphism analysis. Screening of bipolar affective disorder patients for mutations in TRPM2 led to identification of three novel and four known transitions. Two transitions resulted in the substitutions: R755C and A890V. Screening of the coding sequence of ADARB1 did not reveal any mutations except one already known transition. A comparison of the transition frequency in patients and controls does not support association of the detected mutations with bipolar affective disorder. According to our results, bipolar affective disorder may not be caused by mutations in ADARB1. However, this study does not exclude TRPM2 as a candidate gene since we have screened only about 30 per cent of the entire coding sequence of this large gene.

13

Morphological and biochemical changes in human fibroblast lines induced by anthracyclines during apoptosis

84%

Kania K., Dragojew S., Jozwiak Z.

Cellular and Molecular Biology Letters

|

2003

|

tom 08

|

nr 1

We show that treating human trisomie fibroblasts with anthracyclines - aclarubicin, daunorubicin and idarubicin - leads to certain changes in these cells; namely the activation of caspase 3, morphological changes and an increase in the level of intracellular calcium. These results suggest that anthracycline drugs are also able to induce apoptosis in pathological, trisomic cells.

14

Vitamin C decreases intracellular calcium level in human lymphoid cells

84%

Ozturk G., Mulholland C. W., Hannigan B. M.

Journal of Physiology and Pharmacology

|

2001

|

tom 52

|

nr 2

15

Effect of serine proteinase inhibitors on intracellular free calcium rise in human polymorphonuclear leukocytes after stimulation with receptor agonists

84%

Nowak D., Krol M., Bialasiewicz P.

Archivum Immunologiae et Therapiae Experimentalis

|

1998

|

tom 46

|

nr 2

16

Is the NMDA receptor involved in neurotoxicity of pvp-coated silver nanoparticles? Study on primary cultures of cerebellar granule cells

67%

Zieminska E., Stafiej A., Struzynska L.

Acta Neurobiologiae Experimentalis

|

2013

|

tom 73

|

nr 1

Silver nanoparticles (NAg) possess antibacterial properties thus are widely used in many applications in medicine, life sciences and biotechnology. Nanoparticles can be found in vertebrate brain, but little is known about their neurotoxicity. The aim of this study was to investigate how NAg can contribute to neuronal cell death. In the study primary cultures of rat cerebellar granule cells (CGC) were used. We tested hypothesis concerning the role of glutamatergic NMDA receptors in NAg-evoked neurotoxicity. In our study changes in intracellular calcium (Ca2+) homeostasis, uptake of 45Ca2+, reactive oxygen species (ROS) production, mitochondrial membrane potential and cells viability were investigated. We used commercially available 0.2% polyvinylpyrrolidone (PVP)-coated NAg <100 nm. To avoid sedimentation and agglomeration, before application to the CGC culture, NAg were sonicated with fetal calf serum. NAg were applied in concentration 2.5–75 µg/ml for 10, 30 min or 24 h, depending on experiment. As a pharmacological tool 0.5 µM MK801, a noncompetitive inhibitor of NMDA receptor, was used. After 10 min incubation in the presence of 25–75 µg/ ml NAg dose dependent increase of 45Ca2+ concentration was observed in neurons. This increase was comparable to that evoked by 100 µM glutamate and was completely abolished by MK801. Using fluorescent intracellular calcium indicator fluo3 we observed increase in intracellular calcium level by 200% compared to control, which was partially diminished by MK801. ROS production was measured using fluorescent dye DCF. After 30 min incubation with 75 µg/ml NAg the increase by about 35% over control level was observed and application of MK801 reduced it significantly. Changes in mitochondrial membrane potential were determined using rhodamine (R123). We observed significant decrease in mitochondrial potential during 30 min incubation with different concentrations of NAg and also in this case administration of MK801 was protective. Cells viability was assessed after 24 h incubation with NAg µg/ml alone or together with MK801. Application of MK801 increased neuronal survival from 50% up to 80%. Our results show that excitotoxicity via activation of NMDA receptor, followed by calcium imbalance, destabilization of mitochondrial function and ROS production, seems to be important mechanism involved in neurotoxicity evoked by NAg in cultured neurons. Supported by grant NN401619938.

17

The effects of disodium pamidronate on human polymorphonuclear leukocytes and platelets: an in vitro study

67%

Salvolini E., Orciani M., Vignini A., Di Primo R., Mazzanti L.

Cellular and Molecular Biology Letters

|

2009

|

tom 14

|

nr 3

457-465

Recent reports have indicated that, as well as having antiresorptive effects, bisphosphonates could have an application as anti-inflammatory drugs. Our aim was to investigate whether this anti-inflammatory action could be mediated by the nitric oxide (NO) released by the leukocytes migrating to the site of inflammation. In particular, we investigated in vitro the intracellular calcium concentration ([Ca2+]i), the level of NO released by PMN and platelets, and the PMN myeloperoxidase activity after incubation with disodium pamidronate, since there was a postulated modulatory effect of this aminosubstituted bisphosphonate on leukocytes both in vitro and in vivo. Our data shows that the pamidronate treatment provoked a significant increase in the [Ca2+]i parallel to the enhancement in NO release, suggesting a possible activation of constitutive nitric oxide synthase, while the myeloperoxidase activity was significantly reduced. In conclusion, we hypothesized that treatment with pamidronate could stimulate NO-production by cells present near the bone compartment, thus constituting a protective mechanism against bone resorption occurring during inflammation. In addition, PMN- and platelet-derived NO could act as a negative feed-back signal to restrict the inflammatory processes.

18

Changes of U937 cell viability induced by stress factors - possible role of calmodulin

67%

Wojcik-Piotrowicz K., Kaszuba-Zwoinska J., Rokita E., Nowak B., Thor P.

Journal of Physiology and Pharmacology

|

2017

|

tom 68

|

nr 4

19

Kinetics of calcium ion concentration accompanying transduction of signals into neutrophils from diabetic patients and its modification by insulin

67%

Demkow U., Winklewski P., Potapinska O., Popko K., Lipinska A., Wasik M.

Journal of Physiology and Pharmacology. Supplement

|

2009

|

tom 60

|

nr 5

20

Purine and pyrimidine levels in cultured skin fibroblasts from control and Lesch-Nyhan patients

67%

Hussain S. P., Duley J. A., Connolly G. P.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

Ograniczanie wyników

The calcium sensing receptor modulates H+-ATPase activity in intercalated cells

The role of intracellular calcium in the ischemic myocardial damage

Adenosine 5'-triphosphate - a new regulator of annexin function. A hypothesis

Molecular dissection of fertilisation signalling with the aid of tyrosine-kinase-specific inhibitors

Temperature can prime human peripheral blood neutrophils in a p38MAPKalpha-dependent manner

Crataegus special extract WS 1442: up-to-date review of experimental and clinical experiences

The effect of progesterone on oxytocin-stimulated intracellular Ca2plus mobilisation and prostaglandin secretion in porcine endometrium

Free intracellular calcium [(CA2plus)i] in opioid sensitive cells of the porcine anterior pituitary

Effect of Ca2 plus and La3 plus on the callus differentiation in wheat

Mobilization of intracellular calcium by intracellular flash photolysis of caged dihydrosphingosine in cultured neonatal rat sensory neurones

Hydrogen peroxide in expired air condensate correlates positively with early steps of peripheral neutrophil activation in asthmatic patients

Screening of chromosomal region 21q22.3 for mutations in genes associated with neuronal Ca2plus signalling in bipolar affective disorder

Morphological and biochemical changes in human fibroblast lines induced by anthracyclines during apoptosis

Vitamin C decreases intracellular calcium level in human lymphoid cells

Effect of serine proteinase inhibitors on intracellular free calcium rise in human polymorphonuclear leukocytes after stimulation with receptor agonists

Is the NMDA receptor involved in neurotoxicity of pvp-coated silver nanoparticles? Study on primary cultures of cerebellar granule cells

The effects of disodium pamidronate on human polymorphonuclear leukocytes and platelets: an in vitro study

Changes of U937 cell viability induced by stress factors - possible role of calmodulin

Kinetics of calcium ion concentration accompanying transduction of signals into neutrophils from diabetic patients and its modification by insulin

Purine and pyrimidine levels in cultured skin fibroblasts from control and Lesch-Nyhan patients