Wyniki wyszukiwania

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In vitro effects of polychlorinated biphenyls (PCBs) on the contractility of bovine myometrium from the periovulatory stage of the estrous cycle

100%

Wrobel M., Kaminski K., Kotwica J.

Reproductive Biology

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2005

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tom 05

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nr 3

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In vitro effects of prolonged exposure to quercetin and epigallocatechin gallate of the peripheral blood mononuclear cell membrane

100%

Margina D., Ilie M., Manda G., Neagoe I., Danciulescu-Miulescu R., Purdel C. N., Gradinaru D.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 4

The study aimed to assess biophysical changes that take place in the peripheral blood mononuclear cell (PBMC) membranes when exposed in vitro to 10 μM quercetin or epigallocatechin gallate (EGCG) for 24 and 48 h. PBMCs isolated from hypercholesterolemia patients were compared to those from normocholesterolemia subjects. The membrane fluidity and transmembrane potential were evaluated and the results were correlated with biochemical parameters relevant to oxidative stress, assessed in the patients’ plasma. The baseline value of PBMC membrane anisotropy for the hypercholesterolemia patients was lower than that of the control group. These results correlated with the plasma levels of advanced glycation end products, which were significantly higher in the hypercholesterolemia group, and the total plasma antioxidant status, which was significantly higher in normocholesterolemia subjects. In the case of normocholesterolemia cells in vitro, polyphenols induced a decrease in membrane anisotropy (7.25–11.88% at 24 h, 1.82–2.26% at 48 h) and a hyperpolarizing effect (8.30–8.90% at 24 h and 4.58–13.00% at 48 h). The same effect was induced in hypercholesterolemia cells, but only after 48 h exposure to the polyphenols: the decrease in membrane anisotropy was 5.70% for quercetin and 2.33% for EGCG. After 48 h of in vitro incubation with the polyphenols, PBMCs isolated from hypercholesterolemia patients exhibited the effects that had been registered in cells from normocholesterolemia subjects after 24 h exposure. These results outlined the beneficial action of the studied polyphenols, quercetin and EGCG, as dietary supplements in normocholesterolemia and hypercholesterolemia patients.

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In vitro effect of antiparasitic drugs on the expression of the genes code receptors: AchR and GABA in Anisakis simplex L3 larvae

84%

Lopienska-Biernat E., Polak I., Stryiński R.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

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In vitro effects of luteinizing hormone, progesterone and oestradiol-17beta on leptin gene expression and leptin secretion by porcine luteal cells obtained in early pregnancy

67%

Siawrys G., Smolinska N.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 4

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The in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout [Oncorhynchus mykiss], European eel [Anguilla anguilla] and wels catfish [Silurus glanis]

67%

Malaczewska J., Wojcik M., Wojcik R., Siwicki A. K.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 1

83-88

Lactoferrin (LF) is a glycoprotein found in milk, neutrophil granules, secretions and selected organs of mammals. Lactoferrin exhibits antibacterial, antiviral, fungicidal, immunoregulatory and other functions. Although fish are devoid of this protein and its cell receptors, LF effect on the immune mechanisms of fish has been demonstrated. The objective of this study was to investigate the effect of bovine lactoferrin, applied in vitro, on the activity of head kidney and spleen leukocytes in three freshwater fish species: rainbow trout (Oncorhynchus mykiss), European eel (Anguilla anguilla) and weis catfish (Silurus glanis). The obtained results validate LF beneficial effect on the respiratory burst of phagocytes in rainbow trout and weis catfish despite the fact that the potential killing activity against Aeromonas hydrophila was not stimulated in any of the studied species. Bovine lactoferrin enhanced the proliferation of T-lymphocytes in rainbow trout and European eel, as well as of B-lymphocytes in rainbow trout.

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In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase

67%

Tandogan B., Guvenc A., Calis I., Ulusu N. N.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

Glutathione reductase (GR, E.C 1.6.4.2) is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.

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In vitro effect of peroxisome proliferator activated receptor (PPAR) ligands on prostaglandin E2 synthesis and secretion by porcine endometrium during the estrous cycle and early pregnancy

67%

Bogacka I., Bogacki M., Gaglewska M., Kurzynska A., Wasielak M.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 1

Ograniczanie wyników

In vitro effects of polychlorinated biphenyls (PCBs) on the contractility of bovine myometrium from the periovulatory stage of the estrous cycle

In vitro effects of prolonged exposure to quercetin and epigallocatechin gallate of the peripheral blood mononuclear cell membrane

In vitro effect of antiparasitic drugs on the expression of the genes code receptors: AchR and GABA in Anisakis simplex L3 larvae

In vitro effects of luteinizing hormone, progesterone and oestradiol-17beta on leptin gene expression and leptin secretion by porcine luteal cells obtained in early pregnancy

The in vitro effect of bovine lactoferrin on the activity of organ leukocytes in rainbow trout [Oncorhynchus mykiss], European eel [Anguilla anguilla] and wels catfish [Silurus glanis]

In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase

In vitro effect of peroxisome proliferator activated receptor (PPAR) ligands on prostaglandin E2 synthesis and secretion by porcine endometrium during the estrous cycle and early pregnancy