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1

Comparison of dynamic behavior and maturation of neural multipotent cells derived from different spinal cord developmental stages: an in vitro study

100%
Slovinska L., Szekiova E., Blasko J., Devaux S., Salzet M., Cizkova D.
Acta Neurobiologiae Experimentalis
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2015
|
tom 75
|
nr 1
Neural progenitor cells (NPCs) are characterized as undifferentiated cells with the ability of self-renewal and multipotency to give rise to other cells of the nervous system. In our in vitro study we demonstrate the proliferative and differentiative potential of NPCs isolated from the spinal cord at different developmental stages (embryonal, early postnatal, adult), maintained and expanded within neurospheres (NSs). Using the NSs culture system, we examined the size, number of NSs and their fate when exposed to differentiation conditions. Based on immunocytochemical analyses for cell markers (MAP 2, GFAP, RIP) we evaluated the occurrence of various cell types: neurons, astrocytes and oligodendrocytes. The results show that NSs increased in size during cultivation time via NPC proliferation, but proliferation potential decreased during maturation stages. In addition, NPCs derived from spinal cord developmentally different stages gave rise to a consistent ratio of glial and neuronal progeny (3:1), and adult tissues represent a comparable source of NPCs compared to embryonal and early postnatal tissues. These data provide useful information for large-scale in vitro expansion of NPCs required for potential cell therapy after spinal cord injury.
2
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Content of pyrrolizidine alkaloids (senecionine and senkirkine) in Tussilago farfara L. plants cultivated in vitro

100%
Dreger M., Krajewska-Patan A., Gorska-Paukszta M., Pieszak M., Opala B., Gryszczynska A., Adamczak A., Mikolajczak P. M., Buchwald W.
Herba Polonica
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2012
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tom 58
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nr 4
Tussilago farfara L. (family Asteraceae) is a valuable medicinal plant that has been used as a cough suppressant and as an antibacterial and anti-inflammatory drug. Mucopolysac charides, flavonoids, sterols, phenolic acids and pyrrolizidine alkaloids (PAs) are the main active compounds of coltsfoot. Due to hepatotoxic properties of some pyrrolizidine alkaloids, raw materials that contain PAs should be monitored and determined. The aim of present work was to establish nodal cultures of Tussilago farfara and to determine the content of senecionine and senkirkine in plants propagated in in vitro conditions. Eleven clones of coltsfoot derived from Polish natural populations were established. Rhizome buds were used as explants for the initiation of in vitro cultures on MS (Murashige and Skooge) medium. Every six weeks the shots and leaves were collected and dried. The HPLC method was applied for the identification and determination of senecionine and senkirkine. Content of pyrrolizidine alkaloids varied significantly depending on origin (population). An average sum of alkaloids (senecionine and senkirkine) ranged from 1.23 to 10.47 mg/100g d.w. that corresponds to 0.0013–0.011%, respectively.
3

Callus tissues of Rhodiola kirilowii [Regel] Maxim. - dynamic of growth and active compounds production

67%
Krajewska-Patan A., Dreger M., Buchwald W., Gorska-Paukszta M., Mielcarek S., Baraniak M., Mscisz A., Furmanowa M., Mrozikiewicz P. M.
Herba Polonica
|
2009
|
tom 55
|
nr 3
222-230
Rhodiola Kirilowii (Regel) Maxim. (Crassulaceae) is a traditional medicinal plant used in North Asia and China, especially in the cardiopulmonary disorders in the hypoxic conditions induced by high altitude. The presented results are the part of the investigations carried out in the Branch of Medicinal Plants of the Institute of Natural Fibres and Medicinal Plants in cooperation with the Department of Biology and Pharmaceutical Botany, Medical University in Warsaw on R. Kirilowii plants and tissue cultures. The aim of recent study was to determine the growth dynamics and active compounds production during the cultivation of callus tissues of R. Kirilowii on solid/liquid media. Tissue cultures of R. Kirilowii shown the ability to produce all the active compounds determined in the roots of plants of Polish origin. It is worth emphasizing, that rosavins, according to known literature, were not detected in roots of plants growing in Asia. The best time for collection the tissues from solid medium was fifth or sixth week of the culture – the tissues were growing dynamically and the contents of the main active compounds was high. The material from suspension should be collected in 12–15 days after inoculation. The obtained results will be applied in future investigations on the use of R. Kirilowii extracts in the experimental hypoxia in rats.
4

Zdolnosci regenarycyjne fragmentow lisci podkladek jabloni i Aronia melanocaroa [Michx.] Elliot

67%
Rybczynski J. J., Turzynski D.
Prace Ogrodu Botanicznego Polskiej Akademii Nauk
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1992
|
tom 02
37-44
5

Mikrorozmnazanie podkladki jabloni M7 poprzez stymulacje pakow pachwinowych w warunkach in vitro

59%
Plazuk E., Kromer K.
Prace Ogrodu Botanicznego Uniwersytetu Wrocławskiego
|
1999
|
tom 05
423-432
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