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Samples of morbid bovine tissues obtained from slaughterhouse were submitted to histopathological examination. Neoplastic growth within the peripheral nerves was observed microscopically, with the presence of Antoni A and Antoni B patterns, and sporadically Verocay bodies. Additionally, immunohistochemical examination of the sections revealed positive reaction to vimentine, neuron specific enolase, protein S100, and glial fibrillary acidic protein. The results indicate that the examined neoplasms may belong to the type of benign peripheral nerve sheath tumours called schwannoma.
The aim of this paper is to describe the case of a 10-year-old Siberian husky with a right upper lip neoplastic tumour and a metastasis to a submandibular lymph node. Histopathological examination with application of immunohistochemistry confirmed neoplastic outgrowth with an inflammatory form of histiocytoma and a high proliferative potential. Local progression of the neoplastic disease occurred despite the removal of the primary tumour and neoplastically infiltrated lymph node.
This paper describes laparoscopy-guided prostate biopsy experiments in 13 dogs. Biopsy material was evaluated histopathologically to diagnose causes of prostatic gland enlargement. Laparoscopic biopsy was performed in male dogs diagnosed with prostatic gland enlargement, based on clinical symptoms and results of rectal examinations.
In some legal proceedings, the species identification of animal on the basis of fragments of biological material is extremely difficult. This applies both to closely-related and to distant species characterized by similar morphological features. In such circumstances, methods of molecular biology are used, whose evidential value is definitely not in doubt. Histopathological scraps may also have to be used for identifying tests. The aim of the present study was to verify the possibility of using DNA analysis in determining the species of animals on the basis of biological material contained in archival histopathological samples. The examined material consisted of twenty-eight histopathological preparations stained with hematoxylin and eosin. The samples had been prepared from the liver, kidney, spleen, and skeletal muscles. Their age varied from one to seventeen years. Specimens (from twelve species) were identified by inputting sequences in the Barcode of Life Database species identification tool on the basis of the similarity percentage figure from the BOLD report. It was found that genetic tests can effectively identify animal species through the analysis of biological material from histopathological samples.
The effect of adriamycin on the behaviour of CD4⁺ and CD8⁺ cells in the course of trichinellosis in mice has been studied. The animals infected with 200 larvae per mouse were administered intraperitoneally adriamycin (Adriblastin from Farmitalia) at 1st and 28th day post infection (dpi), in a dosis of 0.2 mg. The mice were killed weakly for 6 weeks and then at the 60th dpi. The examinations were made on histologic sections from the spleen, mesenteric lymph node, jejunum and masseter muscle using immunofluorescent and immunoenzymatic methods with monoclonal antibody. The mice receiving adriamycin exhibited more CD8⁺ cells in the intestinal mucosa and by the end of the experiment also in the muscles in comparison with the control animals, which, however, did not affect the course of the infection.
The aim of this study was to investigate the effects of subchronic exposure to atrazine on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) in Danio rerio. Juvenile growth tests were performed on D. rerio according to OECD method No. 215. For 28 days, fish at an initial age of 30 days were exposed to the environmental atrazine concentration commonly detected in Czech rivers (0.3 μg/L) and a range of sublethal concentrations of atrazine (3.0, 30.0 and 90.0 μg/L). The results showed decreasing growth rates and morphological changes in the liver (dystrophic lesions of hepatocytes) at 90.0 μg/L of atrazine. The environmental concentration of atrazine in Czech rivers did not have any effect on fish growth and development of histopathological changes in D. rerio. The value of NOEC was 30.0 μg/L and the value of LOEC was 90.0 μg/L.
Thirty nine canine adnexal tumours were histologically and immunohistochemically analysed. They were classified as trichoblastomas, trichoepitheliomas, sebaceous adenomas and epitheliomas, hepatoid gland adenomas and epitheliomas, and aporcine adenocarcinomas. Moreover, hepatoid gland angioadenoma and angioepithelioma were recognised. Studies of follicular tumours revealed coexpression of cytokeratin MNF116 and LP34, but sebaceous and hepatoid gland tumours as well as apocrine adenocarcinomas showed differences in the expression of both cytokeratins. All the tumours were negative for vimentin except two hepatoid gland adenomas in which coexpression of vimentin and cytokeratin was observed. Positive reaction for neuron-specific enolase (NSE) was observed in follicular tumours, whereas expression of α-smooth muscle actin (α-SMA) was found only in apocrine adenocarcinomas. Moreover, the presence of NSE observed in hepatoid gland adenomas was considered as non-specific just as both glial fibrillary acidic protein in sebaceous gland tumours and hepatoid gland adenomas, and also S100 protein in sebaceous adenomas. All the examined tumours showed lack of expression of both desmin and factor FVIII. Thus, among the used antibodies, cytokeratins, vimentin, NSE, and α-SMA play a main role in the evaluation of canine adnexal histogenesis.
The aim of the study was histopathological characteristics of mink females showing a tendency to decrease body condition in a perinatal period against a background of selected parameters of antioxidant state. The minks were divided into two groups according to body condition scoring system (BCS). The blood was collected twice: after weaning and at the end of the production cycle. The serum activity of oxidative state enzymes was determined. Anatomic and histopathological examinations were conducted after the production cycle. Samples of the liver, kidneys, small intestine, and ovaries were fixed in 10% buffered formalin and stained with haematoxylin-eosin and Sudan IV. The histopathological examinations revealed hyperaemia of the liver and kidneys, with large content of blood in the capillary and central vessels of obese females. The kidney structure was normal in animals of experimental group. On the other hand, kidneys of control group animals showed degenerative changes in the epithelial cells of the tubules of the excretory part, especially of the proximal tubules. Against the background of the obtained results, it should be stated that lower activities of selected enzymes with anatomical and histopathological changes were obtained in the animals with worse body condition.
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