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Cashew is a tropical tree with immense commercial value and the use of its various parts in the confectionary, food, herbal and tanning industry has gained tremendous importance. Thus, the development of quality control method for routine analysis of various products of this widely used plant would be a great help to analysts. The phytochemistry of this plant has not been explored in detail. Anacardium occidentale Linn. (cashew) is reported to contain many antioxidant and polyphenolic compounds. However, there have been no reports published for the HPTLC analysis of the extracts of cashew leaves. Hence, an attempt has been made to develop a HPTLC method for estimation of catechin from leaf extracts. The present paper reveals a rapid high performance thin layer chromatographic method developed for quantitation of catechin from extracts of cashew leaves. The chromatographic parameters such as solvent system, development time, saturation time, detection wavelength were optimized. The mobile phase toluene: ethyl acetate: methanol: formic acid (6:6:1:0.1v/v/v/v) gave the best resolution for various components. The separation of various components and quantitation of amount of catechin was successfully carried out from extracts of cashew leaves. The aqueous extract of leaves contained a higher amount of catechin as compared to ethanol extract. The presence of tannins and phenolics was visualised as bluish-black bands with 5% alcoholic FeCl3 as visualising agent. The method can prove to be a rapid, sensitive and economic alternative as compared to other chromatographic methods especially HPLC for detection of catechin in various food products and herbal formulations.
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The development of regional herbal market

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The process of EU integration faces a lot of possibilities for pre-border companies to develop new business relations. There is an opportunity to develop a cluster of herbal product companies which operate across Bielowieża Savage forest. The development of regional herbal market could positively influence the sale strategy in Belarus and Poland by marketing activities coordination.
The understanding of the pharmacokinetic properties of synthetic drugs as well as herbal preparations used in treatment of many diseases offers a real opportunity to eliminate or minimize the interaction between the active substances. This is of particular clinical importance because it can decrease or increase the action of drugs what is the major cause of failure of pharmacotherapy. Understanding the molecular basis of interaction of substances with enzymes of drug metabolism is extremely helpful in treatment planning and its practical application. The experimental and clinical studies aimed to complete the characterization of the mechanisms of many factors that modulate the activity of cytochrome P450 enzymes, can provide a lot of evidence guaranteeing the safety of herbal products.
The aim of the research was to evaluate the mercury concentrations in common herbal products. There were 55 products examined. All of them, with one exception, contained less than 0.02 mg Hg/kg, which is not dangerous for human's health, according to con­temporary medical knowledge. Achieved results indicate that herbal products, in majority originated in Polish industry, do not give any alarming premises that would indirectly indicate the mercury pollution of natural or agricultural area.
The objective of this study was to determine the effect of dietary supplementation with a blend of organic acids, zinc oxide and the B-Safe® herbal product on nutrient digestibility, nitrogen balance and growth performance in weaned piglets. Nutrient digestibility was determined by a simple balance method, on 24 weaners (PIC) with average body weight of 28 kg, divided into four groups of six animals each. A five-day experimental period was preceded by a seven-day adjustment period. A production trial was carried out on 1279 weaned piglets that were fed four experimental diets: a control diet without feed additives (A), a diet supplemented with a blend of organic acids at 5 kg t⁻¹ (B), a diet supplemented with zinc oxide at 3 kg t⁻¹ (C), and a diet supplemented with the B-Safe® herbal product at 3 kg t⁻¹ (D). The experiment lasted 19 days. A mashed starter diet was offered ad libitum. The body weights of piglets and feed intake were determined at the beginning and at the end (day 19) of the experiment. The inclusion of an organic acid blend, zinc oxide and the B-Safe® herbal product in weaner diets highly significantly improved the digestibility of crude protein, crude fat (P ≤ 0.01) and organic matter (P ≤ 0.05). Nitrogen retention was higher in weaners fed a diet supplemented with zinc oxide at 3 kg t⁻¹ (group C) than in control group animals (20.15 vs. 17.59 g, P ≤ 0.01). The feed conversion ratio (FCR) was highly significantly lower in weaned piglets fed zinc oxide or B- Safe® at 3 kg t⁻¹, compared with the control group (1.50 and 1.47 vs. 1.70 kg kg⁻¹).
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