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1

Microbial purity assessment of herbal raw materials by bioluminometric method

100%

Mietkiewicz-Capala A., Malm A., Bartnik M., Glowniak K., Michalski A., Bielawska-Drozd A., Bartoszcze M.

Herba Polonica

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2004

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tom 50

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nr 3-4

The paper presents the application of the bioluminometric method to assess microbial purity of some herbal raw materials, such as: Lavendae Flos, Hippocastani Flos, Crategi Inflorescentia, Altheae Folium, Betulae Folium, Salviae Folium, Solidaginis Herbae, Chelidonii Herbae and Taraxaci Herbae. The method is based on determination of ATP level originating from bacterial cells. All the raw-materials were examined with FP VI method in order to compare these methods.

2

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Effect of provenance and water stress on biomass and polyphyllin content in the medicinal plant Paris polyphylla Smith var. yunnanensis

75%

Wu X., Zuo, Zhang Q., Wang Y.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 2

Water stress and provenance could affect the secondary metabolites synthesis and accumulation in herbs. Thus, this study explored the effect of soil water moisture and provenance on the growth of Paris polyphylla Smith var. yunnanensis (PPY). Three provenances (Jinping, Luquan and Weixi in Yunnan, China) of PPY samples were grown in different soil water moisture conditions [0.80, 0.70 and 0.50 field capacity (FC)] during Dec. 2015 to Sep. 2017. Results showed that the highest biomass weight was presented in 0.70 FC for Luquan and Weixi samples. Biomass weight for Jinping provenance presented a decreasing tendency with the decreased soil water moisture and the highest biomass were shown in 0.80 FC. However, quantitative analysis revealed that the total content of polyphyllin increased with decreasing the soil water moisture for Jinping and Weixi samples. The highest total content of polyphyllin in rhizome was inclined to show in Jinping samples, while the stem and leaf tissues were shown in Weixi samples. Additionally, results of ANOVA combined with PCA indicated that the difference among these three provenances were significant. Correlation analysis results revealed that 0.50 FC induced the competitive relationship occurrence for polyphyllin distribution. Thus, 0.70 FC was the most suitable soil-water condition for PPY growth. Besides, provenance collected from Jinping could consider as a good quality germplasm. Consequently, this study might provide a preliminary foundation for irrigation project formulated and provenance screened for PPY cultivation.

3

Phenolic acids in rhizomes and herb of tormentil (Potentilla erecta L.)

75%

Geszprych A., Roslon W., Weglarz Z.

Herba Polonica

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2003

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tom 49

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nr 3-4

4

Content of hypericin in herb, seeds and preparations from St.John`s wort [Hypericum perforatum L.]

75%

Ludwiczuk A., Wolski T., Zwolan W.

Herba Polonica

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2002

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tom 48

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nr 4

Moisture, ash and sum of hypericin compounds in herb and seeds of St. John’s wort were determined. Moreover were estimated and compared the content of sum of hypericin compound in gallenic and pharmaceutical preparations with producer’s requirements. The content of sum of hypericin compounds in Hyperici herba and Hyperici semen amounted to 68.67 mg% and 9.19 mg%, respectively. Hypericin content in liquid alcoholic preparations ranged from 0.3 mg% for Succus Hyperici up to 2.17 mg% for Psychotonisol. For solid preparations produced in capsules form, content of sum of hypericin compounds amounted to 0.18 mg/caps for Hyperplant and 1.19 mg/caps for Perhip.

5

Non-destructive method for internal quality determination of Belgian endive [Cichorium intybus L.]

75%

Quenon V., De Baerdemaeker J.

International Agrophysics

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2000

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tom 14

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nr 2

6

Attempt to develop an assortment of herbaceous honey-productioning plants to be used for the improvement of bee pastures on idle lands

75%

Koltowski Z., Jablonski B.

Journal of Apicultural Science

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2001

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tom 45

7

Phenolic constituents of centaury [Centaurium erythraea Rafn.] herb

75%

Sharaf M., El-Ansari M. A., Weglarz Z., Geszprych A.

Herba Polonica

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2003

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tom 49

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nr 3-4

In the centaury herb collected from natural sites in eastern Poland the total content of flavonoids (0.28%), phenolic acids (2.50%) and tannins (1.25%) were determined. From the methanol extract of the raw material four flavonoid compounds were isolated and identified as quercetin 3,3'-dimethylether, kaempferol 3-0-neohesperidoside, isorhamnetin 3-0-glucoside and kaempferol 3-0-rhamnoside-7-0-rutinoside. The last-mentioned compound seems to be a unique one. On the basis of GC of isolated fractions of free phenolic acids and those revealed after alkaline and acid hydrolysis, thirteen phenolic acids were identified. In the fraction of free phenolic acids the main compounds were: protocatechuic, vanillic, p-hydroxybenzoic, m-hydroxybenzoic and syringic acids. In the fraction obtained after alkaline hydrolysis the dominant acids were p-hydroxybenzoic and p-coumaric one. The first one was also the principal acid among those revealed after acid hydrolysis.

8

Effect methanolic extract of Achyranthus aspera on biochemical status of Albino mice

63%

Reddy C. V., Kamble A.

International Letters of Natural Sciences

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2014

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tom 09

The effect of administration of Methanolic extract of Achyranthsu asper on biochemical parameter of mice were investigated. This drug induced significant reduction in the cholesterol level and amount of RNA. But significant increase in the amount of glycogen at 25 mg/kg and no significant reduction in the amount of glycogen at 50 mg/kg. This drug also induced increase in the level of alkaline phospatese and decrease in the level of acid phosphatase, protein and DNA, but these changes are statistically not significant. Protein 25 mg shows 4.22 ±0.112 and 50 mg shows 4.05 ±0.085. Cholesterol 25mg shows 0.5 ±0.04 and 50 mg shows 0.39 ±0.038. Glycogen 25mg shows 0.288 ±0.011 and 50mg shows 0.248 ±0.177. DNA 25mg shows 0.109 ±0.039 and 50 mg shows 0.085 ±0.035, RNA 25mg shows 0.38 ±0.036 and 50 mg shows 0.25 ±0.031

9

Does fish flour and calcium improve mentha development, enzyme activities and phenolic compounds under high salinity

63%

Chitilova M., Yucel N. C.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 2

The use of natural and biodegradable material (such as fish flour) counteracts stress as cheaper and safer alternative for toxic chemicals (such as pesticides). The effect of calcium and fish flour (Ca and FF) (single or in combination) to improve plant tolerance against salt stress was studied. Sterilized mentha seeds were imbibed in each treatment: FF (10 g mL–1), Ca (1, 3%) applied alone and in Ca+FF-combination shaking for 24 h at 150 rpm. Changes in the antioxidants (carotenoids, phenolic, and flavonoid), enzymatic system (superoxidase – SOD, guaiacol-dependent peroxidase – GPX and phenylalanine ammonia-lyase – PAL) and lipid peroxidation levels of mentha seedlings were investigated under salt stress. It was suggested that Ca and/or FF had positive regulation effects on the key enzyme activities related to phenolic compounds biosynthesis and individual phenolic contents under salt stress. Additionally, the mentha plants developed from Ca+FF-combined pre-treatments showed better response to salinity than either Ca or FF single pretreatment. Suppression of salt injury by Ca+FF pre-treatment reduced the LPO levels, increased enzyme activities and promoted total flavonoid and phenolic contents. Ca+FF-combined pre-treatment of mentha seeds seem to be a reliable, not-expensive and easy procedure to enhance plant salt tolerance and to gain more biomass.

10

Medicinal based plants: a call to nature

63%

Awotedu O. L., Ogunbamowo P. O., Chukwudebe E. P., Ariwoola O. S.

World News of Natural Sciences

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2020

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tom 31

11

Ethnobotanical study of traditional herbal plants used by local people of Seshachalam Biosphere Reserve in Eastern Ghats, India

63%

Reddy A. M., Babu M. V. S., Rao R. R.

Herba Polonica

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2019

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tom 65

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nr 1

Introduction: Ethnobotany is the study of medicinal plants used by local people, with particular importance of old-styled tribal beliefs and information. Ethnobotanical studies focus on ethnic knowledge of Adivasi people and development of data bases on ethnic knowledge but also focuses on preservation and regeneration of traditional beliefs and maintenance of traditional knowledge. Objective: The aim of present study is to highlight the traditional actions of herbal plants used by inborn Yanadi community of Seshachalam Biosphere Reserve, Eastern Ghats of Andhra Pradesh, India. Methods: The ethnobotanical field survey was conducted according to the methods adopted by some authors. In-depth interviews, interactions were conducted with tribal physicians of Yanadi, Nakkala and Irula as well as other tribes practicing and experiencing the use of plant-based medicine. A normal inquiry form was used to gather the appropriate data on herbal plants and their usage of inborn people’s lifestyle. Extensive consultations among local people and detailed documentation of the usage of plants were carried out in 2014–2017. The aged outmoded opinions and imposts of indigenous people conceded on by word of opening were documented. Results: A total of 266 medicinally used plant species belonging to 216 genera and 88 families were recognized with help of inborn herbal healers. The study also chronicled the mode of herbal arrangements, mode of the use of herbal plants in various disorders. The study exposed that native people of Seshachalam Biosphere Reserve have good medicinal information and also have preserved plant-based medicinal system of their ascendants used all their diseases. Most of medicinal plants are used in the treatment of indigestion, snake bite and skin diseases. The authors feel that this type of study certainly helps identify ethnic leads for drug development in future. Conclusions: The ethnobotanical investigation of Seshalam Biosphere area has revealed that the tribes possess good knowledge on plant-based medicine but as they are towards in advanced exposure to transformation, their information on traditional uses of plants is slowly getting eroded. The authors plead for intensive crosscultural studies involving all ethnic tribes in the country for prioritizing or short listing of ethnic leads for various disorders for ultimately developing global level drugs for human welfare and economy development.

12

Documentation of traditional knowledge of herbal plant in Kalvarayan Hills, Vallupuram District, Tamil Nadu

63%

Nithyadevi J., Sivakumar R.

International Letters of Natural Sciences

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2014

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tom 07

The study was carried out to assess the traditional knowledge of herbal plants in a Kalvarayan Hills, Villupuram District, Tamil Nadu. It was carried out through face to face interview with respondent, collection and identification of herbal plants in the hills. A total number of 60 species of herbal plants were recorded during a series of folk botanical surveys at a hill at Villupuram District, Tamil Nadu. A majority of these species are herbaceous angiosperms, followed by trees and shrubs. The most common part of the plant used in preparing for herbal medicine is the bark, leaves, roots, stem, flowers, and fruits. Mostly the plants are used for general health, to treat dermatological complaints, reproductive system, abdominal problems and fever compared to other ailments. The most common method of herbal preparation is poultice, followed by decoction and infusion. Thus, more medicinal plants are used topically than orally.

13

Impact of some fungicides on mycelium growth of Colletotrichum gloeosporioides [Penz.] Penz. and Sacc.

63%

Filoda G.

Pestycydy

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2008

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nr 3-4

109-116

The object of the research work were an isolate of Colletotrichum gloeosporioides causing anthracnose on Hypericum perforatum L. (St. John’s Wort) as well as three fungicides belonging to different chemical groups: Sarfun 500 SC, Amistar 250 SC and Gwarant 500 SC. The studies on the inhibiting mycelium growth effect were carried out in vitro on the media amended with the fungicides at concentrations corresponding to field recommended doses for control of anthracnose of many vegetables and ornamental plants and additionally in values double increased and decreased them. The results showed that Sarfun 500 SC even at the twice reduced dose (0.05%) concentration was most effective against this pathogen. Amistar 250 SC at the lower concentration (0.05%) demonstrated low antifungal activity but the effect of 0.1% concentration was significantly higher. In the case of the fungicide Gwarant 500 SC the effective concentration was 0.4% whereas recommended dose is 0.2%. The Sarfun 500 SC and Amistar 250 SC can be therefore regarded as a prospective means of limiting growth and development of Colletotrichum gloeosporioides and protecting St. John’s Wort from this pathogen.

14

Nutritional value and mineral concentrations of sweet basil under organic compared to chemical fertilization

63%

Naiji M., Souri M. K.

Acta Scientiarum Polonorum. Hortorum Cultus

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2018

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tom 17

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nr 2

Safe production of leafy vegetables is more important than other horticultural crops due to their significant contribution in health promotion of consumers. In present study, the growth and nutritional quality of sweet basil plants (Ocimum basilicum L.) were evaluated under some organic compared to chemical NPK fertilizing treatments. A non cultivated soil was used in the experiment, and treatments were arranged in completely randomized design with four replications. Manure in 20 and 40% of pot volume, vermicompost in 20 and 40% of pot volume, biophosphate, NPK fertilizer and a no fertilizing control were applied to plants. The results showed that plant growth and biochemical characteristics were differently affected by organic and chemical fertilization treatments. Vermicompost particularly in 20% of pot volume resulted in highest growth and quality parameters, while the lowest values recorded in 40% manure treatment. The leaf content of ascorbic acid, essential oil, protein and minerals (P, K, Mn, Cu) were highest in 20% vermicompost, whereas leaf content of Mg, Fe and Zn was highest in 40% vermicompost treatment. Leaf nitrate content was significantly reduced in all organic treatments than NPK fertilization. Biophosphate also improved the yield and quality traits compared to unfertilized plants, while manure in both quantities reduced most of the traits (leaf vitamin C, oil yield and protein content) except the content of phenols. This indicates that processing as vermicompost can be a better way of manure application for basil production.

15

Volatile oil constituents of alecost [Tanacetum balsamita L. ssp. balsamitoides [Schultz-Bip.]] growing wild in North-West of Iran

63%

Hassanpouraghdam M. B., Tabatabaie S. J., Nazemiyeh H., Vojodi L., Aazami M. A.

Herba Polonica

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2009

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tom 55

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nr 1

53-59

The hydrodistilled essential oil of aerial parts of Tanacetum balsamita L. ssp. balsamitoides (Schultz-Bip.) Asteraceae growing spontaneously in Tabriz (North-West of Iran) was analyzed by GC-MS. Twenty-three components comprising 96.83% of the total essential oil were identified. Oxygenated monoterpens (87.93%) were the major class of identified components. Sesquiterpene hydrocarbons (6.66%) were the second class. Main monoterpene constituents of the aerial parts essential oil were carvone (49.11%), α-thujone (24.6%), β- thujone (2.68%) and 1,8-cineole (2.59%). β-bisabolene (4.44%) a sesquiterpene hydrocarbone was also present in significant amounts. According to the volatile oil profile, T. balsamita L. ssp. balsamitoides (Schultz-Bip.) plant studied in the present experiment was belong to the carvone/α-thujone chemotype that produces appreciable amounts of β-bisabolene.

16

Improvement of silymarin content in cell cultures of Silybum marianum by copper sulphate elicitor

63%

Elsharnouby M. E., Hassan F. A. S.

Acta Scientiarum Polonorum. Hortorum Cultus

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2018

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tom 17

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nr 2

Silybum marianum L. (Milk thistle) extracts are the main source of silymarin that is a mixture of various flavonolignan (silybin (silibinin), silydianin and silychristin). Silymarin of milk thistle has a hepatoprotective activity for liver cirrhosis and chronic inflammatory. Silybum marianum regeneration from hypocotyl explants and evaluation of their callogenesis, growth and total flavolignan (silymarin) upon copper sulphate (as abiotic elicitor) elicitation was targeted. Copper sulphate (CuSO4) was applied in concentrations of 0, 3, 5, 7 and 9 µM to elicit the silymarin production in cultures. The elicitation periods used in this study were 2, 4, 7, 14 and 28 days. Half-strength MS medium recorded better results relative to full-strength MS one and seed incubation in the darkness at room temperature resulted in rapid germination and reached to the gar lid after 10 days. Callus fresh and dry weights as well as growth index were gradually increased with increasing the copper sulphate concentration till 5 µM while decreased thereafter at any elicitation period. With the increase of the elicitation period, the increase of the previous parameters was observed. Flavonolignan (silymarin) was positively correlated with CuSO4 levels since all levels of copper sulphate significantly enhanced its content in relative to the control. Additionally, more silymarin was accumulated after 4 or 7 days and the accumulation significantly decreased when the elicitation period reached 14 days more. The highest silymarin (flavolignan) content (11.79 and 11.67 mg g–1 DW) was obtained when 5 or 7 µM copper sulphate levels were combined with 4 days elicitation period, being about five-fold of the control.

17

The use of pulsated acoustic field for extraction of bioactive compounds from roots of valerian (Valeriana officinalis L.)

63%

Kobus Z., Guz T., Kusinska E.

Teka Komisji Motoryzacji i Energetyki Rolnictwa

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2008

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tom 08

18

Mineral composition and nutritional properties of Trachystemon orientalis (L.) G. Don populations in the Central Black Sea Region of Turkey

63%

Ozer M. O., Aksoy M.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 4

This study was conducted to determine the mineral contents and some nutritional properties of Trachystemon orientalis L. growing in the Central Black Sea Region, Turkey, and that is consumed as vegetable. Surveys and field studies were started during the spring semester of 2011 (April–May) and they were collected from Samsun and Ordu, where they are intense. In the study, it is was observed that pH, dry matter, content of ash, N, protein, vitamin C, macro- and microelements examined in Trachystemon orientalis varied considerably. The pH, dry matter, ash, N, protein and C content of the plants ranged from 6.61 to 6.88, 13.0 to 22.1%, 9.2 to 17.0%, 2.3 to 3.3%, 14.1 to 20.3 % and 0.12 to 39.03 mg/100g, respectively. Mineral analysis showed that Trachystemon orientalis contained considerably high amounts of potassium (3883.8 to 5791.4 mg/100g), phosphorus (339.7 to 540.9 mg/100 g), calcium (159.4 to 432.4 mg/100g), magnesium (108.0 to 176.4 mg/100 g), iron (10.7 to 63.1 mg/100 g), sodium (22.1 to 66.3 mg/100g), copper (0.6 to 1.5 mg/100 g), manganese (1.5 to 3.6 mg/100g) and zinc (2.3 to 7.6 mg/100g). Mineral compositions of the plants varied significantly depending on the genotypes. T. orientalis was determined as abundant in contents of vitamin C, minerals and protein.

19

HPLC determination of ursolic and oleanolic acids in Lamii albi flos

63%

Wojciak-Kosior M., Sowa I.

Herba Polonica

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2009

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tom 55

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nr 4

25-31

Lamium album (Lamiaceae) is a herb used in therapy of various diseases. Oleanolic and ursolic acid, bioactive triterpenes which often occur together in Lamiaceae family can influence the biological activity of this plant. A HPLC method with DAD detection was developed for the quantification of these compounds in L. albiflos. The best separation was achieved on RP18 column using mixture of acetonitrile, water and \% phosphoric acid (90:10:0.5 mNN) as a mobile phase, at 0.6 mlVmin flow rate and at a temperature of 10°C. Established calibration curve (r>0.999), precision (RSD values ranged from 0.2 % to 1.2 %), recovery (98.4-101.1 %), detection limit (0.12 /Jg/mL for oleanolic acid, 0.13/Jg/mL for ursolic acid) and quantification limit (0.42 /Jg/mL and 0.43 /Jg/mL, respectively) were found to be satisfactory for the proposed method. The determined contents of oleanolic and ursolic acid in L. albiflos were 33.9 /L/g/g and 112.3 ¿ig/g of dry herb, respectively.

20

Environmental and ecological aspects of cultivation of selected energy and herbal plants propagated by in vitro culture

63%

Tomaszewska-Sowa M., Figas A.

Infrastruktura i Ekologia Terenów Wiejskich

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2014

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nr IV/3

There has been a growing interest over the last years in techniques using alternative and pharmaceutical plants due to their wide potential applications. These species are characterised by valuable and multidirectional usable features, due to which their practical application is superior to their physiognomic features. These plants may provide a raw material for production of medicines, cosmetics or may be used for animal feeds. Moreover they may be used as ornamental, melliferous and energy plants. Owing to their generally low soil requirements they are used in the reclamation of degraded land. Among the species characterized by multidirectional use are Silphium perfoliatum, Helichrysum arenarium and Stevia rebaudiana. These plants are characterized by a high content of active compounds and a number of pro-health characteristics. Because raw materials for production of medicines and cosmetics should be standardized, cultures maintained under controlled conditions are a good source of seedlings.

Ograniczanie wyników

Microbial purity assessment of herbal raw materials by bioluminometric method

Effect of provenance and water stress on biomass and polyphyllin content in the medicinal plant Paris polyphylla Smith var. yunnanensis

Phenolic acids in rhizomes and herb of tormentil (Potentilla erecta L.)

Content of hypericin in herb, seeds and preparations from St.John`s wort [Hypericum perforatum L.]

Non-destructive method for internal quality determination of Belgian endive [Cichorium intybus L.]