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The aim of the study was to investigate the effect of acidification and heat on the survival of Clostridium spores in third category animal by-products. Poultry minced meat and minced fish waste, contaminated with Clostridium sporogenes IW 1306 spore suspension, were subjected to the combined action of formic acid and high temperature of different values. To assess the efficiency of examined hygienization method, the spore number in biomass samples after acidification and heat treatment was estimated. For this purpose a 10-fold dilution series in peptone water was prepared and heat-treated at 80°C for 10 min. After cooling-down, one milliliter of each dilution was pour-plated onto DRCM medium solidified with agar. Statistical analyses were performed using the Statistica software. The results of the microbiological analysis show that lowering the pH to about 4 results in increasing the sensitivity of Clostridium sporogenes spores to a high – but not exceeding 100°C – temperature. The fastest inactivation of spores occurred after heating the acidified biomass at a temperature of 90°C. In the case of meat the 48-hour exposure to formic acid allowed the thermal inactivation of the spores within 15 minutes. The investigated method of hygienization may guarantee not only the inhibition of spores germination, but also enables their total elimination, reducing the epidemiological risk related to animal by-products handling.
Adsorption of common bile acids namely lithocholic (LCA), dexycholic (DCA) and cholic (CA) acids and cholesterol by fiber of carrots, cabbage and apples raw and after heat treatment, respectively, was estimated. It was shown that degree of bile acids adsorption depends on kind of the raw material, type of heat treatment and type of bile acids. Fiber carrots had higher sorption than cabbage and apples. Fiber of baked vegetable and apples had significantly higher sorption capacity in compare with boiled and steamed plants material. Lithocholic acid was adsorbed in higher degree than DCA and CA. The cholesterol was bound in the highest degree by fiber cabbage on compared to fiber carrots and apples. Heat treatment of plants material, especially baking, increased the sorption of cholesterol by fiber cabbage as well as carrots and apples.
Hyperthermia can modulate the action of many anticancer drugs, and DNA repair processes are temperature-dependent, but the character of this dependence in cancer and normal cells is largely unknown. This subject seems to be worth studying, because hyperthermia can assist cancer therapy. A 1-h incubation at 37°C of normal human pe­ripheral blood lymphocytes and human myelogenous leukemia cell line K562 with 0.5 juM doxorubicin gave significant level of DNA damage as assessed by the alkaline comet assay. The cells were then incubated in doxorubicin-free repair medium at 37°C or 41°C. The lymphocytes incubated at 37°C needed about 60 min to remove com­pletely the damage to their DNA, whereas at 41°C the time required for complete re­pair was shortened to 30 min. There was also a difference between the repair kinetics at 37°C and 41°C in cancer cells. Moreover, the kinetics were different in doxorubicin-sensitive and resistant cells. Therefore, hyperthermia may significantly affect the kinetics of DNA repair in drug-treated cells, but the magnitude of the effect may be different in normal and cancer cells. These features may be exploited in cancer chemotherapy to increase the effectiveness of the treatment and reduce unwanted ef­fects of anticancer drugs in normal cells and fight DNA repair-based drug resistance of cancer cells.
The aim of the present study was to establish the influence of raw and cooked (fresh and frozen) green cauliflower on some biochemical parameters (levels of total cholesterol, HDL, LDL and VLDL cholesterol and triacylglycerols) in rats. The experiment did not show any effect of cauliflower addition to diet on body weight of rats. The data indicated that AIN-93G diet containing 1% cholesterol increased the level of triacylglycerols and LDL + VLDL fractions in comparison with AIN-93 diet alone. Levels of total cholesterol were lower in animals fed the diet with addition of fresh cooked cauliflower as compared to rats given hypercholesterolemic diet with addition of raw vegetable.
Probiotic strains of L. casei, L. acidophilus, L. helveticus, B. animalis, B. lactis and two strains of B. bifidum were examined for their survival at pH 3.0 and 3% bile salts. Additionally, the viability of L. casei cells was tested in presence of pepsin, trypsin, chymotrypsin, alpha-amylase and lipase, and after heating at 90ºC for 30 min. It was found that all pre-treatments caused significant decrease in bacteria survival. The most sensitive strain for stress factors was B. bifidum 1. Using Caco-2 monolayer culture as the in vitro adhesion model, the significant reduction of adherence after thermal treatment, digestion with pepsin, trypsin and chymotrypsin, and bile salts were observed. The inhibition of bacteria adhesion after thermal denaturation and proteolysis proved an hypothesis that adhesion factors are of proteinaceous origin.
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