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Growth rate and concentrations of sulfates, sulfides, proteins and glucosamine were analyzed during long-term (over 60 days) incubation of Desulfotomaculum acetoxidans DSM 771. To imitate natural conditions, incubation was done in obligate anaerobic conditions in three series, without stirring or shaking. In the first 2-3 days of incubation (lag phase), only a decrease of the sulfate level occurred. Between days 2 and 7 of incubation (logarithmic increase phase, log phase) the growth rate and levels of proteins and glucosamine increased significantly. Simultaneously the amount of dissimilated hydrogen sulfide began to increase. Hydrogen sulfide content in parallel samples treated with lysozyme was much higher. Between days 7 and 18 a plateau ascribed to the stationary phase was observed. After 2 weeks of incubation a certain reduction of the measured substances was observed, but from days 20 to 24 the growth rate again increased (‘post-stationary’ phase). The high coefficients of correlation (for individual series 0.6735; 0.7245; 0.8217) between proteins and sulfide levels and control tests done with standards (albumin and Na2S) suggest that H2S and probably sulfides react with proteins and presumably with peptidoglycan. This could explain cumulation of sulfide and its decrease in the post-stationary phase.
Low molecular mass, heparin-binding proteins from seminal plasma play an important role in gametes interaction whereas plasmatic Zn2+-binding proteins stabilize chromatin and plasmalemma structures and protect spermatozoa in the female reproductive tract. By means of affinity chromatography the heparin- and Zn2+-binding proteins were isolated from boar seminal plasma and both preparations were analyzed by reverse HPLC. Most of the proteins bound to heparine and Zn2+-ions were classified as spermadhesins. Three fractions binding exclusively Zn2+ were isolated. They differ in amino-acid composition, content of glucosamine and content of protein components revealed by SDS/PAGE.
A glucose-nonfermenting Gram-negative bacterial strain isolated from bronchofiberoscope used for examination of the patients suffering from pulmonary diseases was subjected to phenol-water extraction. Lipopolysaccharides (LPS) isolated from the water and the phenol phase differed in fatty acid composition. Both contained xylose, glucose, glucosamine and components typical for LPS, namely heptose, 3-deoxyoctulosonic acid (Kdo) and 3-hydroxymyristic acid. The presence of sphingosine in all LPS preparations classifies the strain to the genus Sphingomonas.
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