Wyniki wyszukiwania - AGRO

1

An efficient method of genomic DNA isolation from plant tissues

100%

Strozycki P. M., Legocki A. B.

Acta Biochimica Polonica

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1995

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tom 42

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nr 3

The manuscript describes an easy method of isolation of plant genomic DNA. This method allowed us to isolate substantial amounts of good quality DNA from lupin (Lupinus luteus) tissues. The described method also appeared to be useful for genomic DNA isolation from tissues of other plants.

2

Comparative mapping of Brassica and Arabidopsis

88%

Sadowski J., Quiros C. F.

Journal of Applied Genetics

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1996

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tom 37A

3

The Kinetica Project: mapping and tracking of protein kinases by 2D gel analysis

88%

Pelech S.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

4

Bioinformatic and physical analysis Arabidopsis and Brassica genomes

88%

Ziolkowski P., Sadowski J.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

5

The model plant Arabidopsis thaliana: Implications from analysis of gene redundance to genome functional plasticity

88%

Sadowski J., Babula D., Kaczmarek M., Ziolkowski P., Stanko A.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

6

Scanning of Geotrichum candidum genome by RFLP-PCR of rDNA and RAPD

75%

Piegza M., Barszczewski W., Witkowska D., Stempniewicz R., Robak M.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2006

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tom 09

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nr 3

7

Introduction to molecular biology of influenza A viruses

75%

Szewczyk B., Bienkowska-Szewczyk K., Krol E.

Acta Biochimica Polonica

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2014

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tom 61

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nr 3

8

FISH mapping in cattle (Bos taurus L.) is not yet out of fashion

75%

De Lorenzi L., Molteni L., Parma P.

Journal of Applied Genetics

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2010

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tom 51

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nr 4

Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.

9

Organizacja genomow wazniejszych wirusow porazajacych ziemniak [Solanum tuberosum L.]

75%

Muchalski T.

Biuletyn Instytutu Ziemniaka

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1994

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nr 44

33-45

10

A preliminary analysis of the region with limited variability in C-repeats of rabbit DNA

75%

Plaza G., Szemraj J., Bednarek A., Anderson P., Szala S.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1988

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tom 36

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nr 4-6

11

Replication of equine herpesvirus type 1 in equine dermal cells transfected with Bam HI[G] restriction fragment of EHV-2 genome

75%

Dzieciatkowski T., Chmielewska A., Turowska A., Tucholska A., Banbura M. W.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 1

In previous experiments, we have demonstrated that the presence of equine herpesvirus 2 (EHV-2) enhanced plaque formation in cell cultures infected with equine herpesvirus type 1. To determine whether a specific region of the EHV-2 genome is responsible for this effect, we have constructed a library of Bam HI fragments of the EHV-2 genome ligated into pcDNA plasmid. Equine dermal (ED) cell cultures were subsequently transfected with the constructs, passaged 5 times, tested for the presence of the plasmids and infected with EHV-1 at MOI=0.01. Only in cultures transfected with the pcDNA/Bam HI[G]construct, designated Δ2/4, the mean number of plaques at 24 hrs p.i. was approximately 10 times higher than in non-transfected controls. Virus titers in culture supernatants as well as in freeze-thawed cells were 4- and 5-fold higher, respectively, than in non-transfected cultures. These differences were observed only at 24 hrs p.i. At 48 hrs p.i. cultures were completely destroyed and, surprisingly, the virus titer was slightly lower in the supernatant of transfected cells. However, the titer of EHV-1 in freeze-thawed culture was exactly the same as in the control. These results suggest that the presence of Bam HI[G] fragment of the EHV-2 genome stimulates (accelerates?) plaque formation only at earlier stages of infection but does not influence the total yield of EHV-1 at 48 hrs p.i. The exact mechanism of this stimulation remains unclear and further experiments are necessary to determine the role of putative EHV-2 proteins encoded by Bam HI [G] fragment of the EHV-genome.

12

Studies on changes in specific rye genome regions due to seed aging and regeneration

75%

Chwedorzewska K. J., Bednarek P. T., Puchalski J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

The aim of this study was to identify the genetic changes in rye seeds induced by natural aging during long-term storage and successive regeneration cycles under gene bank conditions. Genomic DNA from four rye samples (cv. Dańkowskie Złote), varying in their initial viability and having gone through one or three reproduction cycles, were analysed using specific PCR targeting of a secalin locus, and various repetitive fragments defined by the R173 sequence. A statistical analysis of the band frequencies for both secalin and R173.3 primer pairs revealed no changes in their frequencies. Similar data on R173.1 demonstrated significant changes between samples of different initial viability showing a lack of a band of the expected length (987 bp) in progeny originating from low viability seeds lots. These changes were inherited even after three regeneration cycles. Our results may indicate that long-term storage that leads to loss of viability also generates heritable changes in the preserved germplasm. However, it remains to be discovered where these changes occur and whether they are connected with coding or with non-coding DNA regions.

13

CRISPR elements in the Thermococcales: evidence for associated horizontal gene transfer in Pyrococcus furiosus

75%

Portillo M. C., Gonzalez J. M.

Journal of Applied Genetics

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2009

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tom 50

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nr 4

421-430

The presence and distribution of CRISPR (clustered regularly interspaced short palindrome repeat) elements in the archaeal order Thermococcales were analyzed. Four complete genome sequences from the species Pyrococcas abyssi, P. furiosus, P. horikoshii, and Thermococcus kodakaraensis were studied. A fragment of the genome of P. furiosus was flanked by CRISPR elements upstream and by a single element downstream. The composition of the gene sequences contained in this genome fragment (positions 699013 to 855319) showed significant differences from the other genes in the P. furiosus genome. Differences were observed in the GC content at the third codon positions and the frequency of codon usage between the genes located in the analyzed fragment and the other genes in the P. furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea.

14

Evidence for RNA recombination between distinct isolates of Pepino mosaic virus

75%

Hasiow-Jaroszewska B., Kuzniar A., Peters S. A., Leunissen J. A. M., Pospieszny H.

Acta Biochimica Polonica

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2010

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tom 57

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nr 3

Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly; available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools. The genome-wide analyses not only confirm the presence of previously found recombination events in PepMV but also provide the first evidence for double recombinant origin of the US2 isolate.

15

Role of the DNA polymerase III in mutagenesis in the yeast Saccharomyces cerevisiae

75%

Zaborowska D., Baranowska H., Zuk J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1994

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tom 42

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nr 1

16

The nuclear protein p30 specifically interacts with a nuclear matrix attachment region from the rat genome

75%

Fedorov A., Lukyanov D., Rogolinski J., Widlak P., Podgornaya O., Rzeszowska-Wolny J.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 1

In our previous study, a 454 bp DNA fragment was isolated from rat genomic DNA as an element which interacts with nuclear matrix proteins, i.e. a Matrix Associated Region (MAR). Computer analyses revealed that the right half of this fragment, named RME (Rat MAR Element), possesses a high matrix association potential and is likely to be responsible for the matrix association of the whole sequence. RME was used as a probe in an electrophoretic mobility shift assay (EMSA), and with the use of Southwestern blotting, a rat liver nuclear protein which binds specifically to it was identified. Its molecular mass was estimated by SDS-PAGE as 30 kDa (p30). Polyclonal antibodies raised against protein-RME complexes caused a super-shift of specific complexes in EMSA, and bound to p30 in nuclear extracts of rat liver in Western blotting. The immunofluorescence labelling of a rat embryonic fibroblast cell monolayer with anti-p30 antibody revealed a mainly intranuclear pattern of staining.

17

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Is there any mystery of ORPHANs?

75%

Cebrat S., Dudek M., Mackiewicz P.

Journal of Applied Genetics

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1997

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tom 38

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nr 4

We have analysed the coding capacity of ORFs longer than 100 codons found in the yeast genome. Comparing the parameters describing the DNA asymmetry in the set of known genes and the set of all ORFs>100 codons we have found that there are about 4700 coding ORFs in the yeast genome. Since for more than 2300 ORFs recognisable functions have been already found and for about 2000 ORFs homology to known genes has been identified - only about 400 ORFs can be considered as orphans - ORFs without any known function or homology. This finding means that there is no mystery of orphans - a paradox showing that the fraction of orphans has been growing with the growing number of genes with known functions in the yeast genome.

18

Detection of production trait loci in the chicken genome

75%

Hillel J.

Animal Science Papers and Reports

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2004

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tom 22

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nr 1

19

The genetic characteristics Saccharomyces cerevisiae aciplus mutants

75%

Grochowalska R., Machnicka B., Wysocki R., Lachowicz T. M.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2

A series of 30 Saccharomyces cerevisiae aci+ mutants (characterized as acidifying Ogur's glucose medium containing bromocresol purple) were isolated after EMS mutagenesis. All the mutants excreted acid metabolites to the medium after 24 or 48 hours of incubation. The character of the aci+ mutations was defined using classical genetic techniques. Three of the aci+ mutants were studied by molecular genetics techniques.

20

The nuclear matrix and chromosomal DNA loops: is their any correlation between partitioning of the genome into loops and functional domains?

75%

Razin S. V.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 1

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