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On the basis of the faecal examination and necropsies, prevalence of infection, number of species and egg counts (EPG) were estimated. A comparison of EPG of ewes and their lambs showed, that of the group of lambs with the lowest EPG, 25% were from resistant mothers while in the group with the highest EPG - 31.25%
Rhinolophus mehelyi (Mehely's horseshoe bat) is a vulnerable species with an increasingly fragmented distribution. In Romania, populations of R. mehelyi have experienced a dramatic decline over the past 50 years, and the current population size is estimated at only 100 adult individuals inhabiting almost exclusively the Limanu cave. In the present study, we investigated the genetic consequences of population decline for the viability of the remaining population of R. mehelyi in Romania. We sequenced and analyzed a 359-bp fragment of the mitochondrial control region from the only known Romanian population and compared it with two geographically close colonies from Bulgaria. A single haplotype was found in the Romanian population compared to 10 in the Bulgarian population, suggesting genetic isolation.
Six natural populations of sweet clover (Melilotus officinalis) were examined for allelic frequencies at 7 electrophoretically detected polymorphic loci in five enzyme systems: LAP, AP, ME, EST and PX. According to Nei’s statistics, the extent of genetic variability in all populations shows that intra-population genetic variability is expressed by higher values of GST coefficient (mean value for all loci 0.135) which exceeds values of inter-population differences expressed by DST=0.059 coefficient. Gene flow between populations is rather low (Nm=1.60). Hierarchical clustering (UPGMA) of Hedrick’s genetic distances (when examined simultaneously in all the allozymes), demonstrated three groups of populations suggesting certain tendency to geographic connections.
The milk fatty acid (FA) profile is far from the optimal fat composition in regards to human health. The natural sources of variation, such as feeding or genetics, could be used to increase the concentrations of unsaturated fatty acids. The impact of feeding is well described. However, genetic effects on the milk FA composition begin to be extensively studied. This paper summarizes the available information about the genetic variability of FAs. The greatest breed differences in FA composition are observed between Holstein and Jersey milk. Milk fat of the latter breed contains higher concentrations of saturated FAs, especially short-chain FAs. The variation of the delta-9 desaturase activity estimated from specific FA ratios could explain partly these breed differences. The choice of a specific breed seems to be a possibility to improve the nutritional quality of milk fat. Generally, the proportions of FAs in milk are more heritable than the proportions of these same FAs in fat. Heritability estimates range from 0.00 to 0.54. The presence of some single nucleotide polymorphisms could explain partly the observed individual genetic variability. The polymorphisms detected on SCD1 and DGAT1 genes influence the milk FA composition. The SCD1 V allele increases the unsaturation of C16 and C18. The DGATl A allele is related to the unsaturation of C18. So, a combination of the molecular and quantitative approaches should be used to develop tools helping farmers in the selection of their animals to improve the nutritional quality of the produced milk fat.
Species of Gleditsia show considerable morphological variability that makes them difficult to distinguish using either vegetative or floral characters. Honeylocusts, especially the thornless cultivars, are popular ornamental, shade, street, attractive landscape trees. In this study the ISSR technique was used to evaluate the range of genetic variability between seven genotypes of Gleditsia cultivated in Polish dendrological collections [Gleditsia caspica Desf., Gleditsia japonica Miq., Gleditsia japonica Miq. var. korainensis (= G. korainensis Nakai), Gleditsia triacanthos L., Gleditsia triacanthos L. (bulk), Gleditsia triacanthos f. inermis (L.) Zabel (bulk). Forty ISSR primers were tested and 18 were selected for their ability to produce clear and reproducible patterns of multiple bands.A total of 177 loci of 260-2600 bp were amplified, of which 89 (50%) were polymorphic, 14 (8%) monomorphic and 74 (42%) were accession-specific. Accession-specific ISSR loci were obtained for all of the seven accessions tested. A dendrogram generated using the UPGMA, based on a similarity measure of total character difference, showed that the Gleditsia accessions were clustered into two main groups (‘a’ and ‘b’). The first grup –‘a’– included: Gleditsia triacanthos L., Gleditsia triacanthos L. (bulk) and Gleditsia triacanthos f. inermis (L.) Zabel (similarity 0.61–0.75), the second –‘b’– included 2 species: Gleditsia japonica and Gleditsia japonica var. korainensis (similarity 0.43). Analysis of the phylogenetic similarity dendrogram has shown wide range of diversity between studied accessions. The clustering pattern obtained in our experiment was in agreement with the data based on morphological, allozyme and ITS analysis.
Geographically marginal populations are expected to have low genetic variability, which potentially can affect their viability. In Poland Melica transsilvanica Schur reaches the northern limit of its continuous geographical range. Genetic diversity and population genetic structure were analyzed in 15 of its marginal and more central populations using AFLPs. Overall, genetic diversity parameters did not differ significantly, and comparable patterns of genetic variation were found in central and marginal populations. All AFLP phenotypes were unique to particular populations. Unique alleles were fixed in some central and some marginal populations. The percentage of polymorphic loci varied from 1.30 to 5.19 (3.24 average) in central populations and from 0.43 to 5.63 (2.36 average) in marginal ones. Hierarchical analyses of molecular variance (AMOVA) for each species/region combination revealed highly significant differentiation between populations and showed similar partitioning of molecular variance in marginal and central populations of M. transsilvanica (diversity between populations: 93.24% and 93.18%, p < 0.001, respectively). The scattered distribution of suitable species habitats and the predominant selfing breeding system of the species strengthen the effect of selection pressure on fixation of unique loci in individual populations. Marginal populations of M. transsilvanica with unique alleles considerably expand the genetic variation of the species and are therefore valuable for conservation of genetic diversity.
Provenance trials were designed to analyse the quantitative responses of tree species to environmental variables found in different experiment location. However, we have still limited knowledge how natural and artificial selection affects genetic variation of the species populations gather in such experimental sites. We have used bulked DNA-based RAPD and ISJ analysis to investigate genetic diversity and differentiation of Scots pine populations from two Polish locations of IUFRO 1982 provenance trial placed in Kórnik and in Supraśl. Applied categories of DNA markers differed in terms of revealing genetic diversity of the species. Ten RAPD primers applied in the study yielded a total of 75 bands, of which 21 (28%) and 15 (20%) were polymorphic in Kórnik and in Supraśl, respectively. Six ISJ primers revealed 42 bands of which 4 (9.52%) and 14 (33.3%) were polymorphic in Kórnik and in Supraśl, respectively. The genetic diversity and differentiation was low, as expressed by He=0.071 and He=0.085, and by genetic distance values which ranged from 0.0 to 0.240 (on average 0.081) and from 0.017 to 0.188 (on average 0.094) for Kórnik and Supraśl, respectively. Location of provenance trial appeared to have a significant influence on revealed level of genetic polymorphism and pattern of interpopulation differentiation. However, genetic structure found for analysed Scots pine provenances from IUFRO 1982 in Kórnik was also confirmed for Supraśl experimental site. In the light of available data we also discussed the influence of historical migration routes and gene flow on observed genetic variation of the species.
The electrophoretic polymorphism of blood proteins, and karyotypes, were studied in up to 33 captive-bred Persian goitred gazelles Gazella s. subgutturosa (Giildenstaedt, 1780). Allozymes, haemoglobins and serum proteins representing 33 putative genetic loci displayed four biallelic polymorphisms (carbonic anhydrase, malate dehydrogenase, mannose phosphate isomerase, transferrin), resulting in a percentage polymorphism of p = 0.121, and an expected heterozygosity of He = 0.047. Six males had 2n = 31, and seven females 2n = 30 chromosomes. This sex-specific difference was due to an X-autosomal translocation, coupled with a XYiYz sex determining system in males. Neither karyotypes nor protein polymorphism provided evidence to explain the high mortality of newborn goitred gazelles.
Genetic variability of a selected population of Dreissena polymorpha, an invasive species playing a significant role in aquatic ecosystems, was studied. Starch gel electrophoresis was used to analyse 8 enzymatic loci in 200 individuals collected from 20 sites in a lake. The population was found to have 75.0% of polymorphic loci, 2.8 alleles per locus, 3.5 alleles per polymorphic locus, 0.393 coefficient of expected heterozygosity, and 149 genotypes. Zebra mussel clumps were strongly polymorphic; almost every individual had a different genotype. The high polymorphism observed in the D. polymorpha clumps had most likely resulted from external cross-fertilisation and the presence of free-swimming veliger larvae as well as from a considerable heterozygosity of individual bivalves. Genetic variability of the population studied was found to be similar to that of populations inhabiting other Western Pomeranian lakes, including both highly polluted ones and those formed as recently as about 40 years ago. This provides evidence for a mass colonisation of freshwater reservoirs effected by very polymorphic parent populations of D. polymorpha. The literature data on North American zebra mussel populations which invaded that continent about 10 years ago show them to be polymorphic, too, but not as much as European ones.
A South African rehabilitation centre for illegally kept vervet monkeys Chlorocebus aethiops Linnaeus, 1758 required an evaluation of the genetic status of vervet monkeys, to determine whether animals from different geographical areas may be mixed. We studied animals originating from three geographical regions and used biochemical genetic and morphological approaches to address this question. The most prominent trend from allozyme data was derived from the locus Prt-2 (an unspecified serum protein), where each of the three populations could be characterised by the absence or presence of unique alleles. Nevertheless, statistical coefficients indicated little genetic divergence, with genetic distance values of 0.001-0.003 and an overall fixation index value of 0.046. Average heterozygosity did not differ appreciably among populations (2.5-3.3%). The morphological study identified suitable traits, free from the influence of growth allometry, that can be used for inter-population comparisons. No significant morphological differences between con-specific populations were however found. It is concluded that vervet monkeys from the species' wider distribution range is relatively monotypic, but that monkeys from different geographical areas should not be unduly mixed, pending the results of finer grained molecular studies.
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