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An anesthetic combination of medetomidine-midazolam-atropine and propofol was investigated in twenty New Zealand White rabbits. Each rabbit received combined medetomidine at a dosage of 0.2 mg/kg (b.w.), midazolam (0.5 mg/kg b.w.) and atropine (0.5 mg/kg b.w.) intramuscularly for induction of anesthesia. Propofol was administered intravenously - given to effect, and after that by an infusion pump to maintain anesthesia. The influence of the anesthetic combination on the cardiopulmonary system was evaluated by monitoring respiratory and heart rates, blood pressure, and arterial blood gas tensions. The results obtained showed that propofol infusion at a rate of 0.5 mg/kg b.w./min maintained general anesthesia effectively with few side effects on the cardiopulmonary system during 30 minutes. However, slight hypotension, hypercapnia, and respiratory acidosis were associated with infusion of this anesthetic. The recovery of the rabbits from the anesthesia was smooth. Two rabbits died 20 to 24 hours after anesthesia. In conclusion, an anesthetic combination with medetomidine-midazolam-atropine and propofol at the investigated doses was shown to be a safe method to induce and maintain general anesthesia enabling short-term surgical procedures in healthy animals.
Background. Assessments of the efficacy of anaesthetics are usually based on observations of fish behaviour or changes in blood parameters. In this study we attempted to assess the process of anaesthesia, caused by 2-phenoxyethanol and Propiscin (0.2% etomidate), based on recording the heartbeat and the respiratory frequency. Materials and Methods. The effect of two concentrations of 2-phenoxyethanol (0.4 mL·L–1 and 0.8 mL·L–1) and two concentrations of Propiscin (1.0 mL·L–1 and 2.0 mL·L–1) on heartbeat and respiratory frequency of common carp, Cyprinus carpio L., were studied using ECG. Concurrently, during general anaesthesia, the fish behaviour was also observed. Results. The anaesthetic potential of 2-phenoxyethanol and Propiscin at the applied concentrations was comparable. The behavioural reactions caused by the agents were not identical. At the beginning of the exposure, 2-phenoxyethanol produced locomotor agitation and an irritation-like reaction of the respiratory system. Propiscin had no such effect. Both agents induced a decrease in the ventilatory and cardiac frequencies. The reductions caused by Propiscin were simultaneous. The inhibitory effect of 2-phenoxyethanol at the concentration of 0.8 mL·L–1 was much stronger on respiration than on heart rate. Conclusion. The ECG method can be an important and objective tool supplementing data acquired during visual observation of responses to anaesthesia. Its major advantage is the comparativeness of data.
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