Wyniki wyszukiwania

1

A rapid method of agarose gel electrophoresis for separation of different conformational forms of DNA

100%

Ciesielska E., Szmigiero L.

Cellular and Molecular Biology Letters

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1996

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tom 01

|

nr 4

A fast two step method of agarose gel electrophoresis for separation of different conformational forms of DNA is described. In the first step the gel is run in the buffer without ethidium bromide and then the gel is stained with that dye. After staining the second step of electrophoresis is performed in the buffer without ethidium bromide. This two step procedure allows one to receive very good resolution between bands corresponding to relaxed, supercoiled, open circular, and linear forms of DNA.

2

Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS

100%

Borowicz B., Domaniewski J.

Archivum Veterinarium Polonicum

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1994

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tom 34

|

nr 3-4

3

Separation of megabased-sized DNA molecules of Aspergillus niger using pulsed field gel electrophoresis

100%

Coral G., Colak O., Unaldi M. N.

Folia Biologica

|

2002

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tom 50

|

nr 1-2

4

Effect of antisense peptide binding on the dimerization of human cystatin C - gel electrophoresis and molecular modeling studies

86%

Stachowiak K., Rodziewicz-Motowidlo S., Sosnowska R., Kasprzykowski F., Lankiewicz L., Grubb A., Grzonka Z.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 1

Human cystatin C (HCC) shows a tendency to dimerize. This process is particularly easy in the case of the L68Q HCC mutant and might lead to formation of amyloid deposits in brain arteries of young adults. Our purpose was to find ligands of monomeric HCC that can prevent its dimerization. Eleven antisense peptide ligands of monomeric HCC were designed and synthesized. The influence of these ligands on HCC dimerization was studied using gel electrophoresis and molecular modeling methods. The results suggest that all the designed peptides interact with monomeric HCC facilitating its dimerization rather than preventing it.

5

Effect of proflavine and its 2,7-dialkyl substituted derivatives upon initiation of transcription

86%

Piestrzeniewicz M. K., Szemraj J., Gniazdowski M.

Acta Biochimica Polonica

|

1993

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tom 40

|

nr 1

6

Fractionation of DNA isolated from lymphocytes of patients under therapy with the antiblastic drug cis-diamminedichloroplatinum [II] using pulsed field gel electrophoresis

86%

Jaruga P., Zastawny T. H., Tujakowski J., Olinski R.

Acta Biochimica Polonica

|

1993

|

tom 40

|

nr 1

7

Diversity of agronomic traits and total seed protein in black gram Vigna mungo [L.] Hepper

86%

Ghafoor A., Ahmad Z.

Acta Biologica Cracoviensia. Series Botanica

|

2005

|

tom 47

|

nr 2

This study evaluated agronomic traits of 111 genotypes of black gram Vigna mungo (L.) Hepper, mainly from Pakistan, to determine the extent of genetic diversity. Seed proteins were analyzed by SDS-PAGE. High genetic variance was observed for days to flowering, days to maturity, number of branches/plant, number of pods/plant, biomass/plant, grain yield/plant and harvest index, whereas low genetic variance was observed for pod length, seeds/pod and 100-seed weight in both years studied. SDS-PAGE of seed proteins showed low inter-accession diversity and no clear differentiation on the basis of origin or source. The genotypes in one cluster with similar agronomic characters did not necessarily belong to the same origin or source. The lack of a geographic pattern of germplasm collected from Pakistan was probably due to exchange of germplasm between neighbouring regions, or because germplasm represented progenies of the same ancestors. Clustering of advanced breeding lines along with an approved variety in one group revealed that only a portion of the genetic diversity has been exploited for improvement of black gram. It is suggested that the genetic base of cultivated black gram should be broadened by involving diverse parents in the breeding programme. Expansion of the genetic base for black gram breeding might be accomplished by systematic use of germplasm that differs in protein profiles and has better quantitative traits.

8

Advanced methods in photobiology of protists

72%

Hader D.-P.

Acta Protozoologica

|

1991

|

tom 30

|

nr 3-4

9

Native nucleic acid electrophoresis as an efficient alternative for genotyping method of influenza virus

72%

Pajak B., Lepek K.

Acta Biochimica Polonica

|

2014

|

tom 61

|

nr 3

10

Axenic cultures of cyanobacteria - obtaining methods and associated problems

72%

Palinska K. A., Krumbein W. E.

Oceanological Studies

|

1998

|

tom 27

|

nr 1

11

Content and composition of proteins obtained from short-time-predegenerated distal stumps of rat sciatic nerves

72%

Gorka D., Larysz-Brysz M., Lewin-Kowalik J., Krause M., Barski J. J.

Applied Biology Communications. Lublin Scientific Center

|

1994

|

tom 04

|

nr 3-4

12

PCD-related DNA degradation in the nuclei of anther tapetum identified by the comet assay

72%

Lesniewska J., Gradzka I., Szumiel I., Charzynska M.

Acta Biologica Cracoviensia. Series Botanica et Zoologia. Supplement

|

1997

|

tom 39

|

nr 1

13

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Partial characterization of Sunn-hemp mosaic tobamovirus [SHMV] isolated from bean plants [Phaseolus vulgaris L.]

72%

Pospieszny H., Palczewska M., Borodynko N.

Journal of Plant Protection Research

|

2001

|

tom 41

|

nr 2

This work presents some properties of Sunn-hemp mosaic tobamovirus (SHMV) orginally isolated from bean plants. Virus infected host range and induced symptoms that were typical for SHMV. Following plant species distinquished SHMV from tobacco mosaic tobamovirus (TMV): Phaseolus vulgaris, Pisum sativum, Lupinus albus and Lycopersicon esculentum. In immunoblotting the serum against SHMV did not react with TMV and Tomato mosaic tobamovirus (ToMV). The electrophoretical patterns of whole virions and capsid proteins were characteristic for SHMV and different from that of TMV and ToMV.

14

Determination of genome size of Haemophilus influenzae Sb: Analysis of DNA restriction fragments

72%

Kauc L.

Acta Microbiologica Polonica

|

1992

|

tom 41

|

nr 1-2

15

Linkage of two mutant allozymes for Amp2 and Aat2 with marker loci on barley [Hordeum vulgare L.] chromosomes 1 and 6

58%

Kucharska M., Kaczorowska K., Ali E. S.

Journal of Applied Genetics

|

1998

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tom 39

|

nr 2

To saturate barley (Hordeum vulgare L.) genetic maps the linkage relationships of two isoenzyme loci Amp2 (aminopeptydase) and Aat2 (aspartate aminotransferase) with known genetic markers were investigated. Results of the genetic analysis support previous information on the localization of these loci on chromosome 1 and 6, respectively. The following recombination values were estimated: between locus Amp2 and T1-3b translocation break point 13.8 ± 2.1%, between locus Aat2 and translocation T6-7i, 17 ± 3.0% and between locus Aat2 and marker о 24.1 ± 3.0%.

16

Association of lupin mitochondrial plasmid DNA with mitochondrial proteins

58%

Lesniewicz K., Bartkowiak S., Augustyniak H.

Biological Bulletin of Poznań

|

1996

|

tom 33

17

UV effects on photosynthetic oxygen production and chromoprotein composition in a freshwater flagellate Cryptomonas

58%

Haberlein A., Hader D. P.

Acta Protozoologica

|

1992

|

tom 31

|

nr 2

18

Pulsed field gel electrophoresis of three microsporidian parasites of fish

58%

Amigo J. M., Gracia M. P., Salvado H., Vivares C. P.

Acta Protozoologica

|

2002

|

tom 41

|

nr 1

19

Screening of Pisum sativum L. germplasm against Erysiphe pisi Syd.

58%

Nisar M., Ghafoor A., Khan M. R., Qureshi A. S.

Acta Biologica Cracoviensia. Series Botanica

|

2006

|

tom 48

|

nr 2

Powdery mildew (Erysiphe pisi Syd.) significantly reduces the yield and quality of pea all over the world. Screening of a broad range of germplasm revealed three highly resistant genotypes (Fallon, PS99102238 and PS0010128); eleven (Shawnee, Lifter, Franklin, PS610152, PS810240, PS710048, PS610324, PS810191, CGN3273, CGN3272, and PS9910188) showed symptoms after inoculation but the infection was not severe and recovery was rapid. Powdery mildew caused 86% loss to the germplasm, and the severity of the disease was associated with various phases. The pathogen inhibits seed development in the pod. Severe natural infection is expected to eliminate susceptible germplasm, some of which may have valuable, unique characteristics. The screening data were used to explore the relationship between susceptible and resistant genotypes, and between genetic diversity and geographic patterns. Seed protein assays did not sort genotypes by geographic pattern or disease resistance. It is suggested to transfer genes conferring disease resistance and economic yield to one genotype.

20

Differentiation and identification of Stagonospora nodorum and S.avenae using restriction fragment length polymorphisms

58%

Ueng P. P., Cunfer B. M., Bergstrom G. C., Chen W.

Hodowla Roślin, Aklimatyzacja i Nasiennictwo

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1994

|

tom 38

|

nr 3-4

Ograniczanie wyników

A rapid method of agarose gel electrophoresis for separation of different conformational forms of DNA

Analysis of the low molecular weight nuclear polypeptide by isoelectrofocusing of nuclear proteins first separated by gel electrophoresis in SDS

Separation of megabased-sized DNA molecules of Aspergillus niger using pulsed field gel electrophoresis

Effect of antisense peptide binding on the dimerization of human cystatin C - gel electrophoresis and molecular modeling studies

Effect of proflavine and its 2,7-dialkyl substituted derivatives upon initiation of transcription

Fractionation of DNA isolated from lymphocytes of patients under therapy with the antiblastic drug cis-diamminedichloroplatinum [II] using pulsed field gel electrophoresis

Diversity of agronomic traits and total seed protein in black gram Vigna mungo [L.] Hepper

Advanced methods in photobiology of protists

Native nucleic acid electrophoresis as an efficient alternative for genotyping method of influenza virus

Axenic cultures of cyanobacteria - obtaining methods and associated problems

Content and composition of proteins obtained from short-time-predegenerated distal stumps of rat sciatic nerves

PCD-related DNA degradation in the nuclei of anther tapetum identified by the comet assay

Partial characterization of Sunn-hemp mosaic tobamovirus [SHMV] isolated from bean plants [Phaseolus vulgaris L.]

Determination of genome size of Haemophilus influenzae Sb: Analysis of DNA restriction fragments

Linkage of two mutant allozymes for Amp2 and Aat2 with marker loci on barley [Hordeum vulgare L.] chromosomes 1 and 6

Association of lupin mitochondrial plasmid DNA with mitochondrial proteins

UV effects on photosynthetic oxygen production and chromoprotein composition in a freshwater flagellate Cryptomonas

Pulsed field gel electrophoresis of three microsporidian parasites of fish

Screening of Pisum sativum L. germplasm against Erysiphe pisi Syd.

Differentiation and identification of Stagonospora nodorum and S.avenae using restriction fragment length polymorphisms