Wyniki wyszukiwania

1

Enhancement of pathogenicity of Bacillus thuringiensis by gamma rays

100%

Khan K. I., Jafri R. H., Ahmed M.

Polish Journal of Microbiology

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2004

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tom 53

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nr 3

Studies were conducted on the enhancement of pathogenicity of Bacillus thuringiensis by gamma rays on various species of termites. The results showed that the 72 hrs old and 60 or 70 kr irradiated culture of B. thuringiensis were more pathogenic than the non-irradiated ones. 168 hrs old and 150 kr (1.5 kGy) irradiated B. thuringiensis caused quicker mortality rate than the non-irradiated one as it shows maximum enhancement ratio (1.32).

2

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Mutational changes in Delphinium malabaricum (Huth.) Munz.: a potential ornamental plant

72%

Kolar F. R., Ghatge S. R., Nimbalkar M. S., Dixit G. B.

Journal of Horticultural Research

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2015

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tom 23

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nr 2

3

Mutagenic effect of gamma rays and EMS on seed germination, seedling height reduction and survivability of chick pea (Cicer arietinum L.) var. Co - 4

72%

Umavathi S., Mullainathan L.

International Letters of Natural Sciences

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2014

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tom 11

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nr 1

The effect of gamma irradiation and EMS treatment on seed germination and seedling height of Chick pea (Cicer arietinum. L.). In this regard Co-4 variety of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50, and 60 kR) and EMS (10, 20, 30, 40 and 50 mM) for inducing mutation. The effect of mutagen was observed on the basis of percentage of seed germination, seedling height reduction at 15th day and survivability. From the result it was observed that, the percentage of seed germination, seedling height reduction at 15th day and survivability were significantly decreased with increasing doses/concentrations of mutagen. The effective doses/ concentrations which caused 50 % growth reduction were 40kR in gamma rays and 30 mM in EMS.

4

Effect of gamma rays in induced morphological mutants on M2 generation of chilli (Capsicum annuum L.) var K1

72%

Mullainathan L., Aruldoss T.

International Letters of Natural Sciences

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2015

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tom 03

The present study was carried out to induce morphological mutation in chilli (Capsicum annuum L.) var k1. The seeds were subjected to different treatment levels of gamma rays are 20, 30, 40, 50, 60 and 70Kr along with control. The treated and untreated seeds were raised in a randomized block design to observe the different morphological characters in M2 generation. Several unique and interesting mutants were isolated in this investigation, these independent mutants have differ from control, the most distinct mutants included tall, dwarf, bushy , flower, sterile and pod mutant, was observed in M2 generation.

5

Morphology and Yield parameters and Biochemical analysis of Soybean (Glycine max (L.) Mrr.) Using Gamma rays, EMS and DES treatment

72%

Gopinath P., Pavadai P.

International Letters of Natural Sciences

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2015

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tom 08

Soybean (Glycine max (L.) Merr.) var. Co-1 seeds were treated with physical mutagen namely such as gamma rays and chemical mutagen namely such as Ethyl Methane Sulphonate (EMS) and Diethyl Sulphate (DES). Various concentrations of gamma rays 10, 20, 30, 40, 50 and 60 KR, EMS (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) and DES (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %). The morphological parameters such as days to first flower, plant height, number of cluster per plant, number of pod per plan, seed yield per plant, number of branches per plant, number of leaves per plant, protein content and oil content increasing with increasing level of some doses gamma rays, Ethyl methane sulphonate and Diethyl sulphate treatment in M2 and M3 generation were carried out. A number of mutants were recorded in M2 and M3 generations for one or more traits viz., plant height, maturity, branching, pigmentation, fruit size and yield were isolated in M2 and M3 generation. Mean for various characters increased at mutagenic treatment than control. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of gamma rays 0.5% of EMS and 0.4% of DES treated population with compared to control plants.

6

Induce physical and chemical mutagenesis for improvement of yield attributing traits and their correlation analysis in Chickpea

72%

Raina A., Laskar R. A., Khursheed S., Khan S., Parveen K., Amin R., Khan S.

International Letters of Natural Sciences

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2017

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tom 61

In the current scenario of sky high population and widespread food insecurity, there is an urgent need for productivity improvement of major crops like pulses, one of the most climate friendly and accessible foods for the large poor population of the world. Chickpea is an important pulse crop, having high percentage of nutritional content and is widely used as food across the world. Induced mutagenesis has proved over time as a coherent tool for modern plant breeders to improve the productivity for combating the food insecurity and malnutrition across the globe. Dry and healthy seeds of Cicer arietinum L. var. C-235 were treated with individual and combination doses of gamma rays, sodium azide and hydrazine hydrate to raise the M₁ generation. M₂ seeds harvested from M₁ plants were sown in the nest season. Bio-physiological study in M1 generation showed growth inhibition in the order viz. combination treatment>hydrazine hydrate>gamma rays>sodium azide. In M₂ generation, comparative observations were recorded for morphological variation and quantitative traits to assess the genetic response of the chickpea variety C-235 toward the different concentrations of mutagens. Observations on isolated mutants in M₂ generation revealed that 0.3% HZ + 0.02% SA was most mutagenic while 200 Gy, 0.03% SA and 0.2% HZ were highest in their individual treatment groups. In context to the frequency of morphological mutants studied in M₂ generation, dwarf mutants were found to be highest followed by tall and branching pattern mutants. Highly significant correlation coefficient between yield and other traits showed that the selection for high yielding mutants can be done on the basis of these traits undoubtedly.

7

Mutagenic effectiveness and efficiency of gamma rays and HZ with phenotyping of induced mutations in lentil cultivars

72%

Laskar R. M., Khan S.

International Letters of Natural Sciences

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2017

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tom 64

In mutation breeding, mutagenic effectiveness and efficiency are the base parameters to predict the mutagenic potency of any mutagen. Studies on mutagenic effectiveness and mutagenic efficiency of physical mutagen (gamma rays) and chemical mutagen (hydrazine hydrates; HZ) on two cultivars of lentil (Lens culinaris Medik.), viz. DPL 62 (macrosperma) and Pant L 406 (microsperma) have been reported. Dry and healthy seeds were treated with four doses of each gamma rays (100-400 Gy), HZ (0.1-0.4 %) and their combinations. Frequencies of the induced agro-morphological variations into different phenotypic categories were estimated in M2 population that resulted into identification and isolation of wide range of mutants with altered phenotypes. Data on effectiveness and efficiency of various mutagenic treatments calculated on the basis of the frequency of chlorophyll mutations, which showed effectiveness and efficiency were higher at the moderate doses of gamma rays and HZ, while in case of combination treatments; lower doses were most effective and efficient with few inter-varietal exceptions. Phenotyping of the mutants revealed that growth habits was the most sensitive category to which most of the mutant belongs, followed by leaf and flower/pod/seed in both the cultivars studied. Overall, the screened and isolated mutants with economically important agronomic traits can be further propagated in the subsequent generation for development of elite lentil mutant cultivars.

8

Studies on effect of physical and chemical mutagens in sorghum (Sorghum bicolor (L.) Moench) in M2 generation

72%

Suthakar V., Mullainathan L.

International Letters of Natural Sciences

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2015

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tom 37

Seeds of Sorghum (sorghum bicolor (L.) Moench) Variety CSV – 23. Were mutagenesis with gamma rays and EMS to determine their mutagenic sensitivity in morphological and yield characters. The spectrum of Mutation and induced variability for various quantitative and yield traits were observed in M2 generation. Plant height (cm),total number of leaves, leaf length (cm), leaf width(cm), stem girth (cm),Days taken to first flowering (days),ear head length(cm),ear head width(cm), Grain weight(gm),100 seed weight(gm), Biomass weight(gm).

9

Apoptosis and clonogenic survival in three tumour cell lines exposed to gamma rays or chemical genotoxic agents

72%

Kumala S., Niemiec P., Widel M., Hancock R., Rzeszowska-Wolny J.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

We compared the extent to which apoptosis is induced and clonogenicity reduced in three tumour cell lines - the human melanoma Me45 and promyelocytic leukaemia HL-60, and the rat rhabdomyosarcoma R1 - after exposure to the anticancer drugs etoposide and cis-platinum or to gamma radiation; each induces different types of DNA damage. Cells which readily underwent apoptosis did not necessarily show a correlated loss of clonogenicity; for example, Me45 cells showed the highest sensitivity to all three agents in clonogenic assays but much lower levels of apoptotic cells than R1 or HL-60 cells. These results show that the efficiency of the eradication of clonogenic cells by genotoxic agents does not solely depend on the induction of apoptotic processes, and suggest that the induction of apoptosis and suppression of clonogenicity are independent processes.

10

Induced physical mutagenesis and its effect in cytological behavior of Ashwagandha (Withania somnifera (L.) Dunal)

58%

Bharathi T., Gnanamurthy S., Dhanavel D., Ariraman M.

International Letters of Natural Sciences

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2014

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tom 12

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nr 2

The mitotic effect of physical mutagen gamma rays was observed in the root tip cells of Ashwagandha. The Chromosome analysis has been showed as an important tool for establish variability of the plant seed by use of physical mutagen gamma rays. The gamma rays have of low wavelength and high penetrable power. The plant has tremendous medicinal values and it is known from ancient times. The dry and well matured seeds of ashwagandha were irradiated with different doses of gamma rays viz., 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 KR respectively. The chromosome number of control plant is 2n = 48. The gamma rays affect the normal cytological behavior of ashwagandha species. The chromosomal aberrations increase with increase in the doses of gamma rays to optimum level of 30KR, because it causes changes in the chromosome structure, cellular structure and metabolism of plants. The chromosome aberration like, Sticky metaphase, Precocious moment chromosome, Fragments, Anaphasic bridge, Anaphasic laggard, Telophasic laggard. The present investigation was carried out to study the cytogenetic analysis of the species Withania somnifera. The chromosomal aberration increases with increase in the doses to optimum level (50 KR) of physical mutagen gamma rays.

11

Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes

58%

Kaczmarska M., Kopyscianska Z., Fornal M., Grodzicki T., Matlak K., Korecki J., Burda K.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

We studied the influence of low doses of γ radiation (from 0.04 to 1.8 mGy) on the stability of human red blood cells (RBC) from healthy donors and diabetic patients using absorption spectroscopy. Because of the alteration of many enzymatic pathways in diabetic RBCs resulting in strong modification of the lipid and protein membrane components one could expect that the ionizing γ-radiation should influence the stability of the healthy and diabetic cells in a different way. Indeed, distinct discontinuities and monotonic changes of hemolysis detected in the healthy and diabetic RBCs suggest that various enzymatic and chemical processes are activated in these membranes by γ radiation. Mössbauer measurements showed that only the highest applied dose of γ radiation caused modification of hemoglobin in both types of RBCs.

12

Influence of magnetic field with chemomutagen and gamma rays on the variability of yielding parameters in barley [Hordeum vulgare L.]

58%

Rybinski W., Pietruszewski S., Kornarzynski K.

International Agrophysics

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2003

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tom 17

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nr 2

13

Phosphatidylserine externalization during bone marrow cell death after treatment of mice with WR-2721 and gamma-rays

58%

Mazur L., Bochenek M., Augustynek A.

Folia Biologica

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2002

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tom 50

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nr 1-2

Ograniczanie wyników

Enhancement of pathogenicity of Bacillus thuringiensis by gamma rays

Mutational changes in Delphinium malabaricum (Huth.) Munz.: a potential ornamental plant

Mutagenic effect of gamma rays and EMS on seed germination, seedling height reduction and survivability of chick pea (Cicer arietinum L.) var. Co - 4

Effect of gamma rays in induced morphological mutants on M2 generation of chilli (Capsicum annuum L.) var K1

Morphology and Yield parameters and Biochemical analysis of Soybean (Glycine max (L.) Mrr.) Using Gamma rays, EMS and DES treatment

Induce physical and chemical mutagenesis for improvement of yield attributing traits and their correlation analysis in Chickpea

Mutagenic effectiveness and efficiency of gamma rays and HZ with phenotyping of induced mutations in lentil cultivars

Studies on effect of physical and chemical mutagens in sorghum (Sorghum bicolor (L.) Moench) in M2 generation

Apoptosis and clonogenic survival in three tumour cell lines exposed to gamma rays or chemical genotoxic agents

Induced physical mutagenesis and its effect in cytological behavior of Ashwagandha (Withania somnifera (L.) Dunal)

Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes

Influence of magnetic field with chemomutagen and gamma rays on the variability of yielding parameters in barley [Hordeum vulgare L.]

Phosphatidylserine externalization during bone marrow cell death after treatment of mice with WR-2721 and gamma-rays