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Tomato is sensitive to cold during vegetative growth, fruit set, development, and ripening. We have characterized the effect of cold stress (6°C for up to 48 h) on the transcriptome of Micro-Tom tomato fruits during ripening by subtractive PCR. The cold stress caused modifications in gene expression of housekeeping genes. From a total of 38 genes up-regulated by cold, only one clone - a dehydrin homologue - was related to previously identified cold-stress genes. Phylogenetic analysis showed its clustering with other cold-induced dehydrins, and increased distances from dehydrins activated by abscisic acid. Quantitative expression analysis of tomato dehydrin showed it was activated by cold treatment in leaves and fruits. As dehydrin is a member of the Sl-CBF1 regulon from tomato, we analyzed the cold-responsive transcription factor Sl-CBF1 in mature leaves and ripening fruits stored at 6°C. Leaves of Micro-Tom showed high basal levels of the transcription factor Sl-CBF1, compared to fruits. Cold treatment caused increased levels of Sl-CBF1 expression in leaves but not in fruits of Micro-Tom and Demisem (a commercial cultivar). Tomato dehydrin can be used as a transcriptional marker of cold stress in leaves and ripening fruits. However, our results indicate that the cold response activation of dehydrin gene in tomato fruits is the consequence of an alternative pathway, different from the Sl-CBF1 regulon.
The aim of carried out research was comparing the effect of different pruning time on cropping and fruit quality of several early ripening plum cultivars. The experiment was conducted at Fruit Experimental Station – Samotwór near Wrocław in 2001–2004. Objects of studies consisted of 8 year-old plum trees of ‘Herman’, ‘Čačanska Rana’, ‘Sanctus Hubertus’ and ‘Čačanska Lepotica’ cultivars budded on ‘Myrobalan’ seedlings (Prunus divaricata). The experiment was established in a randomised block design in three replications with six trees per plot. In 2001–2003, time of tree pruning was differentiated. Half of trees was pruned only at the end of August (summer pruning after harvest of fruit) and the other one only at the end of March (dormant pruning before blooming time). Time of pruning had no influence on yielding and chemical composition of fruit. Soluble solids, vitamin C, magnesium, calcium, potassium and phosphorus contents in fruit were similar independently of pruning time. Mean fruit weight was significantly higher from trees with dormant pruning.
Cornelian cherry in Poland belongs to the alternative fruit species. The fruits are suitable for fresh consumption, however they can be used for processing: juice, jams, jellies, syrups, tinctures. Fruits are abundant in mineral elements, vitamin C, organic acids, iridoids, anthocyanins and due to that they have health-promoting significance. In this experiment, the fruit quality of several 16-year-old ecotypes in the Lublin region was compared. They were compared in terms of the beginning of fruit ripening and fruit size (length, diameter, mass), fruit shape index, share of stone in the fruit, SSC, TA, SSC/TA, reducing sugar, dry matter content and anthocyanins content. The color of fruit was evaluated in two dates, visually and using HanterLab spectrophotometer. Cornelian cherry fruits began to mature at the beginning of August and at the latest at the end of August. With the increase of the maturity degree, the SSC, reducing sugars, TA, dry matter and anthocyanins increased significantly. There was no difference in the taste of light red fruits and dark red fruits, which were characterized by similar ratio of SSC/TA. Ecotype No. 11, as the earliest maturing, with big round fruits, dark red colored with the highest content of anthocyanins, could be distinguished. As well as ecotypes No. 4 and 5, ripening in mid-August, and having a high ratio of SSC/TA, providing a good taste. Studied ecotypes could be used in breeding programs to incorporate a wide range of quality and agronomic characteristics into a final cornelian cherry cultivar.
Influence of fruit development and ripening on the changes in physico-chemical properties, antiradical activity and the accumulation of polyphenolic compounds were investigated in Maoluang fruits. Total phenolics content (TP) was assayed according to the Folin-Ciocalteu method, and accounted for 19.60-8.66 mg GAE/gf.w. The TP gradually decreased from the immature to the over ripe stages. However, the total anthocyanin content (TA) showed the highest content at the over ripe stage, with an average value of 141.94 mg/100 g f.w. The antiradical activity (AA) of methanolic extracts from Maoluang fruits during development and ripening were determined with DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging. The highest AA was ob­served at the immature stage accompanied by the highest content of gallic acid and TP. Polyphenols were quantified by HPLC. The level of procyanidin B2, procyanidin B1, (+)-catechin, (-)-epicatechin, rutin and tran-resveratrol as the main polyphenol compounds, increased during fruit development and ripening. Other phenolic acids such as gallic, caffeic, and ellagic acids significantly decreased (p < 0.05) during fruit development and ripening. At over ripe stage, Maoluang possess the highest antioxi- dants. Thus, the over ripe stage would be the appropriate time to harvest when taking nutrition into consideration. This existing published information provides a helpful daily diet guide and useful guidance for industrial utilization of Maoluang fruits.
We examined the response of maturing tomato fruit exposed for 7 days to fluridone (1-methyl-3-phenyl-5-[3-trifluoromethyl(phenyl)]-4(1H)-pyridinone). Fluridone was applied in lanolin paste in the form of a 2–3 mm wide strip from the top to the base of the fruit. As a control, a similar stripe of lanolin was applied in the same way on the opposite side of the same fruit. The content of major carotenoids, as well as flavonoids, and free and bound phenolic acids were determined using a HPLC and HPLC-MS-MS methods. Fluridone almost completely blocked the biosynthesis of lycopene and substantial declined content of ß-carotene and lutein in the tomato fruit. The fluridone caused a decreased content of quercetin, rutin and naringenin, and increased level of epicatechin. The herbicide did not affect the content of p-coumaric acid, but reduced the level of caffeic acid, both free and ester form, and declined the content of free ferulic and chlorogenic acids. Changes in phenolics composition observed for the first time indicate that fluridone interferes with the biosynthesis of further products of the metabolism of p-coumaric acid, both flavonoids and phenolic acids.
Tomato ripening in normal red-fruited cultivar (Fiorin) was delayed by treatment with methyl jasmonate (JA-Me) vapour. A visual scoring system for describing tomato ripening was used. Surface of fruits exposed to JA-Me vapour, increased in yellow and decreased in red as deter mined by HunterLab colour meter. JA-Me significantly altered the firmness of fruits after 21 days storage. Vapour of JA-Me enhanced the level of ß-carotene in outer part (peel with 3 mm pericarp tissue) of fruit, while it had no effect in peeled fruit pericarp. JA-Me treatment decreased the level of lycopene in outer part and pericarp tissue, however, in outer part lycopene content decreased at a higher rate than in pericarp. Amount of tomatine in fruits treated with JA-Me had enhanced four-fold in outer part and by 62% in peeled fruit pericarp as compared with the control.
Badania prowadzono w latach 1998-2004 w Dąbrowicach, w kolekcji gruszy założonej w Sadzie Doświadczalnym Instytutu Sadownictwa i Kwiaciarstwa w Skierniewicach. Ich celem była klasyfikacja zgromadzonych tu odmian pod względem pory dojrzewania owoców. Oceniano drzewa ponad 200 genotypów gruszy, posadzone wiosną 1995 roku w rozstawie 4 x 2,5 m. Wszystkie genotypy były rozmnożone na siewkach gruszy kaukaskiej (Pyrus caucasica). Każdego roku dla badanych genotypów określano początek dojrzałości konsumpcyjnej owoców. Na podstawie uzyskanych wyników oceniane genotypy uszeregowano od najwcześniejszych do najpóźniejszych, wyodrębniając 5 grup różniących się porą dojrzewania owoców. Owoce odmian bardzo wczesnych dojrzewały od 15 lipca do 25 lipca, wczesnych od 26 lipca do 25 sierpnia, średnio wczesnych od 26 sierpnia do 20 września, późnych od 21 września do 10 października, a bardzo późnych po 10 października. Wśród ocenianych odmian najliczniejszą grupę stanowiły grusze późne, których owoce - tak jak owoce odmian bardzo późnych - w kolejnych latach badań zazwyczaj dojrzewały w zbliżonych terminach. Najmniej liczna była grupa odmian bardzo wczesnych, charakteryzujących się - podobnie jak odmiany wczesne i średnio wczesne - dużym zróżnicowaniem pory dojrzewania owoców.
Badano zmiany ilościowe w składzie aminokwasowym białka i w mineralnych składnikach pokarmowych owoców papryki ostrej traktowanej etefonem w początkowej fazie dojrzewania owoców.
Przedstawiono wyniki badań porównania wartości biologicznej papryki pod względem koncentracji karotenoidów w zależności od fazy rozwoju i dojrzałości owoców. Określono poziom β-karotenu i ksantofili w owocach niedojrzałych 20 i 30 dni po kwitnieniu (dpk) oraz w początkowej i końcowej fazie dojrzewania owoców (45 i 60 dpk). Stwierdzono, że czerwone owoce papryki charakteryzują się najwyższym poziomem karotenoidów. Zawierają ponad 19-krotnie wyższy poziom ksantofili w 100 g s.m. i ponad 2-krotnie β-karotenu niż owoce niedojrzałe (30 dpk) oraz 2-krotnie wyższą zawartością tych składników niż w fazie początkowego dojrzewania (45 dpk). Analiza procentowego udziału poszczególnych karotenoidów w czerwonych owocach wykazała, że dominującymi były karotenoidy prowitaminy A (β-karoten i β-kryptoksantyna) - 26,8% oraz czerwony barwnik papryki kapsantyna - 37%. Zastosowanie etefonu stymulowało proces tworzenia karotenogenezy. W fazie pełnej dojrzałości zanotowano wzrost poziomu β-karotenu o ponad 30% w stosunku do zawartości w 100 g suchej masy owoców roślin kontrolnych.
The study was conducted in the 2007-2010 season, in the Pomological Orchard of the Research Institute of Pomology and Floriculture in Skierniewice, Poland. Under assessment was the productive value of 10 foreign strawberry cultivars: 'Camarosa', 'Gloria', 'Ventana', 'Albion' (USA), 'Susy', 'Vima Rina', 'Vima Xima', 'Elianny', 'Nancy' (the Netherlands) and 'Malling Pearl' (England). The standard cultivars were: 'Honeoye' and 'Elsanta'. The cultivars were evaluated in terms of fruit ripening time, marketable yield, fruit size and firmness, soluble solids and vitamin C content, the level of fruit infection by grey mould (Botrytis cinerea) and plant infection by leaf spot (Mycosphaerella fragariae), leaf scorch (Diplocarpon earliana) and powdery mildew (Sphaerotheca macularis). The studies revealed that the earliest to ripen were the fruits of the cultivar 'Ven­tana' and the last those of 'Vima Xima'. The most productive were: 'Elsanta', 'Vima Xima' and 'Nancy'. The largest fruits were produced by: 'Elianny', 'Camarosa', 'Vima Xima', 'Gloria' and 'Albion'. The fruits of the cultivars 'Albion', 'Gloria' and 'Vima Rina' were notable for the highest firmness. The highest amounts of soluble solids were found in the fruits of 'Elianny' and 'Malling Pearl'. The highest amounts of vitamin C were in 'Albion', 'Ventana' and 'Nancy'. Most of the cultivars were characterized by low infection of leaf spot, leaf scorch and powdery mildew. Moder­ately infected by leaf scorch were: 'Ventana' and 'Vima Xima', and by powdery mildew - 'Nancy'. Of all the evaluated cultivars the most useful for cultivation in central Poland is 'Vima Xima' because of its high productivity, high fruit quality and a ripening time that is later than in the other cultivars.
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