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The use of dietary supplements has been observed for many years. Unfortunately, the status of food supplements and main differences between these and drugs remain unknown to most consumers. The purpose of the study was to analyze the opinions of future healthcare professionals on dietary supplements, their behavior concerning food supplementation, as well as their knowledge on the safety of these products.The survey was conducted among 354 future healthcare professionals, using a questionnaire composed of 21 items exploring demographic characteristics, knowledge of supplements, reasons for the use of dietary supplement, and recall of the use.All respondents knew that the purpose of using these products is supplementation. A vast majority of respondents (83.9%) thought that a diet with no supplementation is possible or probably possible. 49.4% of respondents (n=175) declared taking food supplements. The most commonly used ones (68.6%) were products recommended during illness and used to improve the condition of skin, hair, and nails. Moreover, dietary supplements mentioned by students included those containing herbal ingredients, such as: Equisetum arvense, Allium sativum, Panax ginseng, and Ginkgo biloba. Most of respondents buy dietary supplements at pharmacies (89.14%).
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Malatonin in humans

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Melatonin, the hormone of the pineal gland, received a great deal of attention in the last decade because of its availability as over-the-counter drug or food supplement in some countries and suggested role in many vital physiological processes. Melatonin secretion is not restricted to mammals but is also produced in nonmammalian vertebrates, in some invertebrates, and in many plants, with the same molecular structure. The synthesis of melatonin is strictly controlled by lighting conditions and shows a clear circadian rhythm with low values during the daytime and significant increase at night. In this survey the basic data on melatonin significance in human physiology and in pathological processes as well as its possible thrapeutic significance are reviewed and discussed.
Na základě hodnocení výsledků rozsáhlé metaanalýzy zabývající se problematikou vztahu výživy a zdravotního stavu, Světová zdravotnická organizace zařadila výživu k šesti základním faktorům podílejících se na vzestupu prevalence neinfekčních nemocí hromadného výskytu. Nevhodná výživa a nedostatečný pohybová aktivita výrazným způsobem zvyšují riziko vzniku metabolických onemocnění. Správná výživa zahrnující potravinové doplňky zabezpečuje vhodný přívod základních živin, mikronutrientů, minerálů a vitaminů, pozitivně ovlivňuje zdraví na individuální i populační úrovni.
The aim of the study was to assess the effect of the diet supplements BeeTonic, Beeodine and Immunbee solution on the quality of honeybee colonies in the spring. The administration of dough containing Beeodine to bees may have had a positive effect on their vitality, thanks to which they occupied the largest space in the nest (4.1 inter-comb spacer, on average) on April 15, and in late April the occupied space in this group increased almost 2.5 times. The observed colonies had a similar number of cells with brood at the 1st and 2nd measurements. They also showed a similar growth of brood. The colonies given cake with Beeodine were characterized by the highest unit productivity (4.6 kg). Similarly, the production of brood, bees and wax was stimulated by the addition of Immunbee solution (4.3 kg), whereas BeeTonic did not produce such an effect.
Seven commercial food supplements present on the Polish market were evaluated for their in vitro antioxidant capacity. The selected products were in the form of hard gelatin capsules. They contained the extracts from chokeberry, cranberry, blueberry and green tea. The mixture of vitamins and minerals as well as the product containing vitamin C in substantial dose were included into comparison. The products were examined using three methods in order to evaluate their antioxidant capacity: electron paramagnetic resonance (EPR), Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbing antioxidant capacity (ORAC) assays. Total polyphenolic content was determined according to Folin-Ciocalteu method. The results were calculated per capsule. All studied preparations showed antioxidant properties and may provide substantial antioxidant protection. The in vitro antioxidant capacity varied considerably and was associated with the content of polyphenols in the capsule. The supplement with 250 mg of green tea extract was the most potent antioxidant in all assays. Nevertheless it must be remembered that the amounts of extracts were different in encapsulated products. As the quality of extracts and their properties are miscellaneous, there is a need to standardize dietary antioxidant supplements with respect to their antioxidant capacity if effective doses are to be recommended.
Siberian sturgeon weighing 50 ±5 g, cultured in cages stocked at 45 specimens/m3 density and placed in cooling water, were fed Dan-ex 2545 trout feed for 112 days. Experimental treatments differed in the amount of ascorbic acid + glucose mixture (1 : 9) added to the feed. The mixture was applied, in individual treatments, in doses of 0; 1; 5; and 10 g/kg feed, corresponding to ascorbic acid doses of 0; 0.1; 0.5; and 1 g/kg feed. The highest mixture doses resulted in a significant increase in the fish body fat level; however, no significant effect on fish growth was observed.
Strong radical-scavenging activity of Geranium macrorrhizum extracts isolated by using various solvent systems has been reported previously. This study aimed at expanding the knowledge on the bioactivities of antioxidatively active G. macrorrhizum butanol fraction, which was isolated from ethanolic extract (EB), and water fraction, which was isolated from water extract (WW) by measuring their singlet oxygen scavenging properties, as well as preliminary assessment of cytotoxicity and genotoxicity toward mammalian cells. The cytotoxicity (necrosis induction) of the extracts in bovine leukemia virus-transformed lamb kidney fibroblasts (line FLK) was partly prevented by antioxidants and stimulated by the prooxidant BCNU (N,N'-bis(2-chloroethyl)-N-nitrosourea). This indicates that the cytotoxicity of G. macrorrhizum extracts is at least partly attributed to their prooxidant action, presumably due to the formation of quinoidal products of their (auto)oxidation. The latter was evidenced by the nature of the peroxidase-catalyzed oxidation products, which supported DT-diaphorase-catalyzed oxidation of NADPH and participated in conjugation reactions with reduced glutathione. The genotoxic properties were studied using chromosome aberration (CA) and sister chromatid exchange (SCE) tests in human lymphocytes in vitro and Drosophila melanogaster somatic mutation and recombination test (SMART) in vivo. In the CA test, only the highest doses of both fractions significantly increased chromosome aberration frequency. In the SCE test, both fractions induced SCEs in a clear dose-dependent manner. G. macrorrhizum extracts were not genotoxic in the SMART test in vivo. Our data indicate that in spite of the possible beneficial (antioxidant) effects of Geranium extracts, the possibilities of their use as ingredients of functional foods and/or food supplements should be further examined due to their cyto- and genotoxic effects resulting mainly from the action of quercetin-derived components abundant in the extracts.
The aim of this research was to determine the effects of enzyme preparations, lysozyme and lysosubtilin in particular, and those supplemented with vitamins A, C and E, added to cows’ feed, on the somatic cell count and technological properties of milk which are considered primary important in cheesemaking. Three groups of Lithuanian Black and White cows (10 cows each) were fed on a ration supplemented with Neosomatas 1 preparation containing lysosubtilin, 0.02 g and lysozyme, 0.2 g, and Neosomatas 4 preparation (lysozyme, 0.2 g and vitamins A, C and E). The duration of the trial was 10 days. Measurements were conducted on the 4th, 7th and 10th day of the trial and 7 days following the trial. The reduction of somatic cell count in milk was observed during and after the feeding period. The highest effect of the feed supplement was observed in the third group which was fed on Neosomatas 4. The enzyme- and vitamin-based feed supplements were also effective with regard to the technological properties of milk. Changes in the acidity of milk during storage were rather slow. Likewise, the process of milk fermentation was slow, the properties of structures that were formed differed compared with control group of cows. However, the technological properties of milk from cows fed on Neosomatas 4 supplement were better as compared with cows fed on Neosomatas 1 feed supplement. Studies on the enzyme structure formation and the process of syneresis showed that feed supplements enriched with enzymes and vitamins had no effect on the above indices. It was also found that milk under study was characterised by good technological characteristics and therefore was suitable for cheesemaking.
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