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1

Detection of extra virgin olive oil adulteration using fluorescence spectroscopy

100%
Wojcicki K., Grzechowiak S., Sikorska E.
Towaroznawcze Problemy Jakości. Polskie Towarzystwo Towaroznawcze
|
2013
|
nr 1
2

Fluorescence spectroscopy for monitoring thermal and photo-oxidation of cold-pressed vegetable oils

86%
Wojcicki K., Insinska-Rak M., Sikorski M., Khmelinskii I., Sikorska E.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
3

The dependence of fluorescein-PE fluorescence intensity on lipid bilayer state. Evaluating the interaction between the probe and lipid molecules

86%
Kubica K., Langner M., Gabrielska J.
Cellular and Molecular Biology Letters
|
2003
|
tom 08
|
nr 4
The degree of dependence of a lipid bilayer’s surface properties on its conformational state is still an unresolved question. Surface properties are functions of molecular organization in the complex interfacial region. In the past, they were frequently measured using fluorescence spectroscopy. Since a fluorescent probe provides information on its local environment, there is a need to estimate the effect caused by the probe itself. In this paper, we address this question by calculating how lipid head-group orientation effects the fluorescence intensity of Fluorescein-PE (a probe that is sensitive to surface potential). In the theoretical model assumed the lipid bilayer state and the interactions between the charged fluorescent probe and the surrounding lipid molecules was evaluated. The results of this theoretical analysis were compared with experimentally obtained data. A lipid bilayer formed from DPPC was chosen as the experimental system, since it exhibits all the major conformational states within a narrow temperature range of 30°C - 45°C. Fluorescein-PE fluorescence intensity depends on local pH, which in turn is sensitive to local electrostatic potential in the probe’s vicinity. This local electrostatic potential is generated by lipid head-group dipole orientation. We have shown that the effect of the probe on lipid bilayer properties is limited when the lipid bilayer is in the gel phase, whereas it is more pronounced when the membrane is liquid-crystalline. This implies that Fluorescein-PE is a good reporter of local electrostatic fields when the lipid bilayer is in the gel phase, and is a poor reporter when the membrane is in the liquid-crystalline state.
4
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Fluorescence spectroscopy for analysis of beer

86%
Insinska-Rak M., Sikorska E., Czerwinska I., Kruzinska A., Nowacka G., Sikorski M.
Polish Journal of Food and Nutrition Sciences
|
2007
|
tom 57
|
nr 4[B]
5

The study of the interactions between ketoprofen and N-acetyl-L-tyrosine ethyl ester

86%
Zgolak K., Jeszka A., Kabzinski T.
Zeszyty Naukowe. Politechnika Łódzka. Chemia Spożywcza i Biotechnologia
|
2007
|
tom 71
6

Dansylated analogues of the opioid peptide [Dmt1]DALDA: in vitro activity profiles and fluorescence parameters

86%
Berezowska I., Lemieux C., Chung N. N., Zelent B., Schiller P. W.
Acta Biochimica Polonica
|
2004
|
tom 51
|
nr 1
Dansylated analogues of the potent and selective u opioid peptide agonist [Dmt1]DALDA (H-Dmt-D-Arg-Phe-Lys-NH2; Dmt = 2',6'-dimethyltyrosine) were pre­pared either by substitution of Nε-dansyl-a,β-diaminopropionic acid or Nε-dansyl- lysine for Lys4 , or by attachment of a dansyl group to the C-terminal carboxamide function via a linker. All three analogues displayed high u agonist potency in vitro and the C-terminally dansylated one retained significant u receptor selectivity. The three analogues showed interesting differences in their fluorescence emission maxima and quantum yields, indicating that the dansyl group in two of them was engaged in intramolecular hydrophobic interactions. These dansylated [Dmt1]DALDA analogues represent valuable tools for binding studies, cellular uptake and intracellular distribution studies, and tissue distribution studies.
7

Capacity of blood albumins to bind ochratoxin A and roridin A

72%
Grajewski J., Moraczewska J., Fogt K., Twaruzek M.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 05
The study concerned the ability of human (HSA), pig (PSA) and sheep (SSA) blood serum albumins to bind ochratoxin A (OTA) and roridin A (RoA). Fluorescence spectorscopy and cytotoxicity test MTT with swine kidney cells (SK), susceptible to a wide range of mycotoxins, were used for the evaluation of the mycotoxin-albumin complexes. OTA was found to bind strongly to all the studied albumins, but the nature of the binding was found to be different in all three cases. Binding abilities corresponded with the results of the cytotoxicity test MTT. It was not possible to analyze roridin A with the fluorescence method, since this toxin is nonfluorescent. The MTT test results showed that RoA did not bind to the albumins of any of the examined species and was strongly toxic to the cells. The new complexes OTA created with HSA and PSA are so strong that they may be used as biomarkers for diagnostic purposes.
8

Spectroscopic studies on UVC-induced photodegradation of humic acids

72%
Slawinska D., Polewski K., Rolewski P., Plucinski P., Slawinski J.
Electronic Journal of Polish Agricultural Universities. Series Environmental Development
|
2002
|
tom 05
|
nr 2
The effect of UV radiation on absorption, fluorescence spectra and dynamics of phototransformation of five different humic acids (HA) of natural and synthetic origin were investigated. Two types of synthetic HA were prepared: one containing only C, H and O atoms, and another with content of C, H, O and N atoms. Natural HA were also diversified with respect to their origin. We used UV-C radiation (predominantly the wavelength of 254 nm) to provide a wide spectrum of photochemical mechanisms.
9

Modulation of physiological and pathological activities of lysozyme by biological membranes

58%
Trusova V.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
|
nr 3
The molecular details of interactions between lipid membranes and lysozyme (Lz), a small polycationic protein with a wide range of biological activities, have long been the focus of numerous studies. The biological consequences of this process are considered to embrace at least two aspects: i) correlation between antimicrobial and membranotropic properties of this protein, and ii) lipid-mediated Lz amyloidogenesis. The mechanisms underlying the lipid-assisted protein fibrillogenesis and membrane disruption exerted by Lz in bacterial cells are believed to be similar. The present investigation was undertaken to gain further insight into Lz-lipid interactions and explore the routes by which Lz exerts its antimicrobial and amyloidogenic actions. Binding and Förster resonance energy transfer studies revealed that upon increasing the content of anionic lipids in lipid vesicles, Lz forms aggregates in a membrane environment. Total internal reflection fluorescence microscopy and pyrene excimerization reaction were employed to study the effect of Lz on the structural and dynamic properties of lipid bilayers. It was found that Lz induces lipid demixing and reduction of bilayer free volume, the magnitude of this effect being much more pronounced for oligomeric protein.
10

Spectral methods in water analysis

58%
Fijalkowski J.
Oceanologia
|
1981
|
tom 13
11

Ibuprofen-tyrosine (Val-Tyr, Val-Tyr-Val) interactions. Theoretical and experimental studies

58%
Kowalska-Baron A., Brychtova M., Petrovic I., Passos Sene I., Quinones P., Sogorkova J.
Biotechnology and Food Science
|
2011
|
tom 75
|
nr 2
12

Effect of drought stress on changes in chlorophyll fluorescence and spectrofluorescence emission and excitation of leaves of triticale and maize genotypes differing in drought tolerance

58%
Grzesiak M. T., Hura T., Rzepka A., Filek W., Hura K., Skoczowski A.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
13

Impact of the drought stress on changes of spectrofluorescence parameters in maize hybrids differing in drought tolerance

58%
Grzesiak M. T., Grzesiak S., Skoczowski A.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 1 Suppl.
14

UV effects on photosynthetic oxygen production and chromoprotein composition in a freshwater flagellate Cryptomonas

58%
Haberlein A., Hader D. P.
Acta Protozoologica
|
1992
|
tom 31
|
nr 2
15

Cyclic voltammetry and fluorescence spectroscopy studies on the effect of polysialic acid on phospholipid model membranes

58%
Janas T., Nowotarski K., Janas T.
Cellular and Molecular Biology Letters
|
1999
|
tom 04
|
nr 2
16

Effect of artificial UV-B and simulated solar radiation on the flagellate Euglena gracilis: physiological, spectroscopical and biochemical investigations

58%
Gerber S., Hader D. P.
Acta Protozoologica
|
1995
|
tom 34
|
nr 1
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