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The time it takes for ingested seeds to pass through the gut of animals is an important aspect of endozoochorous seed dispersal because it influences seed dispersal distance. Variations in the physical characteristics of seeds, such as their weight, volume, and specific gravity, can affect their movement through the gastrointestinal system of a given animal. We conducted feeding experiments with captive Japanese martens, Martes melampus (n = 4), at Toyama Municipal Family Park Zoo, central Japan to examine the effects of the physical characteristics of seeds on their passage times. The mean (±SD) transit time, mean retention time, and time of last appearance of four different types of commercial seeds were 2.6 ± 0.3 h (range 0.6–5.4), 9.7 ± 1.1 h (3.8–17.3), and 23.8 ± 3.1 h (12.2–51.8), respectively. All of these values are greater than those found during previous experiments conducted with mustelids. Similar to previous studies, however, none of these passage time variables was correlated with the physical characteristics of seeds. Our results thus indicate that martens disperse seeds of different plant species, whose size, volume, and specific gravity all fall within the range of those used in the present study, from parent plants at similar distances.
A 60-day feeding experiment was conducted to study the effects of including 0, 20, 40, or 60 g · kg–1 leaf meal of tetraploid Robinia pseudoacacia (TRLM) in diets on performance, egg quality, and apparent nutrient and amino acid digestibility of hens. Egg mass and egg weight were both higher when feeding 40 g · kg–1 TRLM than the other three diets, the egg shape index linearly increased (P < 0.05). No significant effects of diet were seen on the other hen performance or egg quality parameters. Apparent nutrient and amino acid digestibilities increased at first and subsequently decreased with increasing inclusion of the foliage, but no significant differences were observed in dry matter or glycine. Digestibility of crude protein, ether extract, gross energy and acid detergent fibre increased up to 20 g · kg–1 TRLM and subsequently decreased, and similar trends were observed for 13 amino acids, but the digestibility of neutral detergent fibre decreased up to 40 g · kg–1 TRLM and subsequently increased. A significant linear decrease (P < 0.05) in the digestibility of proline was observed with increasing inclusion of TRLM into the diets. Tetraploid Robinia pseudoacacia leaf meal could be a potential supplementary protein source in laying hen diets at inclusion levels not exceeding 60 g · kg–1.
Feeding experiment on nine feral mink Mustela vison Schreber, 1777 was carried out to find differences in digestibility of particular prey types. The values of correction factors (CF), which show the ratio between consumed prey biomass and undigested remains, were calculated for seven food types. The experiment revealed that results obtained by commonly used percentage of occurrence methods differed from the real intake of food. Food types which were intensively digested were underestimated by percentage of occurrence methods and prey which had hardly digestive elements of their body were overestimated. The calculated values of CF are as follows: crayfish - 14.8, fish - 30.8, frog - 61.3, small passerine bird - 17.2, chicken - 41.3, rodent - 17.3, egg - 687.5. The variability in CF values did not depend on mink individuals but could be explained by the mean weight of prey items eaten by mink. The positive correlation between the prey size and its digestibility was recorded for crayfish, fish and chicken. The biomass of frog, passerine and rodent remains was not related to the body mass of eaten prey. The use of evaluated correction factors enables more accurate estimates of the food composition of wild living mustelids.
Effect of dried pumpkin (Cucurbita maxima D.) supplementation on growth performance, serum biochemistry and parameters antioxidant status of rats. The aim of the studies was to determine the effect of dried pumpkin, used in the diets for rats on parameters of growth, nutrient metabolism and antioxidant status of the animals. The experiment was carried out for 7 weeks with 30 growing male Wistar rats. The animals were classified into three groups, 10 individuals in each group, with the initial body weight of 108 g. The control group (G-0) was fed the semi-synthetic mixture without dried pumpkin additive whereas the experimental groups received the mixture with 5-% (G-5) and 10-% (G-10) additive of the dried pumpkin, Ambar variety. The dry substance was obtained from disintegrated fruits, deprived of seed nests, dried at temperature of 60°C. During the experiment weight gains and feed intake were controlled. After termination of the experiment, the rats were killed by anaesthesia; the blood samples were collected and biochemical indices and indicators of antioxidant status were determined. The dietary treatments had no effects on animal growth and feed utilization. In the animals receiving dried pumpkin in their diets (G-5, G-10) significantly lower level of glucose concentration in serum was found. In group G-0, the higher concentration of triacylglycerolsin relation to group G-10 was recorded. Also, the concentration of total cholesterol in group G-0 was higher in comparison to groups G-5 and G-10. In group G-0, VLDL concentration was also higher in relation to group G-10. In group G-10 vs. groups G-5 and G-0, the higher activity of glutathione peroxidise (GPx) was recorded. Total antioxidant status (TAS) was higher in group G-10 in comparison to groups G-0 and G-5. The effect of the administered diet on indicators of the degree of lipid oxidation was also found. In group G-10 vs. group G-0, thiobarbituric acid reactive substances (TBARS) concentration was lower.
Impact of food type on long term consumption kinetics in group-housed domestic cats (Felis catus). The aim of the current research was to assess the impact of the type of dry food on the long term acceptance in cats, expressed as consumption curves. A group of 14 adult neutered domestic cats were subsequently offered three types of products: economy, medium and premium. The consumption of food has been carefully monitored each day of the experiment, which enabled the drawing of the precise consumption curve. The average daily consumption showed differences between feeding periods (98.33%, 61.17% and 55.04% for premium, medium and economy diet type, respectively). In all groups the monotony effect has been observed, but the relative stability of the consumption has been observed only in cats fed with the premium type of food. The attractiveness of the economy type of food was distinctively low, resulting in a regularly waved consumption curve. It can be concluded, that the prolonged offering of a particular type of complete dry pet food within the limits of metabolizable energy requirements may potentially lead to adverse consequences for cats. The food consumption level that supplies the minimal daily energy amount can likely result in imbalanced macronutrient intake.
A 60-day feeding experiment was conducted in the laboratory to evaluate the interactive effects of dietary protein and carbohydrate levels on growth, feed utilization efficiency, and nitrogen metabolism in rohu, Labeo rohita fingerlings (mean weight 4.06 ±0.08 g). Nine purified diets prepared with 25, 35, and 40 % protein level each having 15, 25, and 35 % carbohydrate were fed to nine different feeding groups at the rate of 3% of body weight in triplicate treatments. Better performance of fish in terms of percent weight gain, SGR, FCR, and PER was observed with increasing percentage of carbohydrate at a given protein level. Protease and α-amylase activities increased with increase in dietary protein and carbohydrate levels, respectively. Glutamate oxaloacetate transaminase (GOT) activities varied significantly in some groups. No significant difference among different groups regarding glutamate pyruvate transaminase (GPT) activity was observed. Ammonia excretion was found to increase with increased consumption of dietary protein and carbohydrate and was highest in the groups of fish fed 40% protein diet suggesting active nitrogen metabolism in these.
The feeding experiment described was run within July 19 – October 18, 1999 on 536 g (ą20 g) mean individual weight carp individuals aged 1+. The fish were kept in cages stocked at a 50 ind. cage–1 density. Experimental treatments differed in the feed offered. The following feeds were used: Dan–ex 1848 (Dan), Carp Grow–ex (Grow), and AT 35 Starter (AT 35). The daily feed doses, amounting to 2.0% metabolic fish weight (W0.8) in each experimental treatment, were applied manually. During the first phase of the experiment (19 July – 13 September), the metabolic growth rate (MGR) values were 17.40; 15.73; and 13.19 g kg–0.8 in the Dan, Grow, and AT 35 treatments, respectively, the respective food conversion ration (FCR) being 1.15; 1.27; and 1.52. During the second phase of the experiment (14 September – 18 October), the respective MGR values amounted to 15.17; 15.77; and 13.24 g kg–0.8, while the corresponding FCR values were 1.32; 1.27; and 1.51.
Influence of application of (1,3)-(1,6)-β-D-glucans and mannans on production results of sows and piglets. The aim of the study was the assessment the production results and health status of sows and their offspring modulated by supplementation of (1,3)-(1,6)-β-D-glucans and mannans. The experiment was carried out in farrowing and nursing sector.It was performed using crossbreed (Polish Large White × Polish Landrace) pigs. The additive of (1,3)-(1,6)-β-D-glucans (G) or (1,3)-(1,6)-β-D-glucans and mannans (GM) was introduced to the feed for sows from the 85th day of gestation and for their offspring from the 22nd (after weaning) to the 90th (moving to the fattening sector) day of life. Production results were estimated basis on: changes of body weight of sows during lactation and their offspring during the experimental time, average feed intake, feed conversion ratio, mortality. To estimate health status blood samples from sows and their offspring were taken to determine the total protein level and its fraction in serum. The obtained results indicate that the additive of G or GM to lactation sows and weaned piglets can usefully influence on catabolism process in sows, growth performance and health status in piglets.
Effect of dietary arginine supplementation on body weight changes and productivity of sows.The objective of this study was to determine whether provision of additional arginine to pregnant and lactating sows could influence body weight changes in females during reproductive cycle as well as reproductive and rearing performance of the piglets. The study included 36 F1 crossbred sows (Polish Landrace ´Polish Large White), including 12 primiparous and 24 multiparous sows, which after insemination were randomly allocated to experimental group E (6 primiparous – P and 12 multiparous – M) and control group C (6 P and 12 M). Sows from both groups received complete diets, which were fed to meet requirements. Sows from group E were supplemented with amino acid (L-arginine 98%) at 0.3 kg/ton for pregnant sows (from 4 weeks after insemination) and at 0.5 kg/ton for lactating sows. C sows were not supplemented. Feeding group (C, E) had a significant effect on sow body weight at weaning (P ≤0.01), while lactation (P, M) had a significant effect on sow body weight at mating and weaning, and also on body weight change during the weaning-to-mating period (P ≤0.01). The number of piglets born to primiparous sows from group E vs C was higher by 1.3 piglets (11.11%) (P >0.05). 28-day-old weaned piglets, reared by primiparous sows, were significantly heavier than the progeny of multiparous sows (P ≤0.01), by 0.76 kg/animal (10.08%) in group C and by 0.97 kg/animal (12.63%) in group E. Piglets from group E vs C were heavier at weaning by 0.35 kg (4.22%) when born to primiparous sows, and by 0.14 kg (1.86%) when born to multiparous sows. No group ´lactation interaction has been shown for any of the parameters studied. It seems appropriate to supplement pregnant and nursing primiparous sows with dietary arginine.
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Use of hop cones in growing beef cattle nutrition

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Use of hop cones in growing beef cattle nutrition. The objective of this study was to determine the effect of the supplementation of bull diets with hop cones on growing bull performance, the concentrations of blood analytes, including liver enzymes. Twenty-four growing bulls of Slovene autochthonous Cika breed (BW 373 kg; age 329 days) were randomly allocated to 6 pens (4 animals/pen). Before the start of experiment all animals received the same basic TMR which was afterwards either not supplemented (control; 2 pens) or supplemented with 50 g/animal (H50; 6 g of hop dry matter (DM)/kg diet DM; 2 pens) or 100 g of hop cones/animal (H100; 11 g DM/kg diet DM; 2 pens) daily. Bulls were weighed at the start of the experiment and then again after 30 and 60 days of experiment and average daily gain (ADG), dry matter intake (DMI) and feed-to-gain ratio (F:G) were calculated. At each weighing day, the blood samples were taken from each bull and plasma glucose and serum non-esterified fatty acids (NEFA), beta-hydroxy butyrate (BHBA), urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyl transferase (GGT) were determined. The inclusion of hop cones in the diet did not have any effects on DMI, ADG or F:G ratio and did not change BHBA, urea and GGT concentrations. ALT level was lower (P = 0.025) after 60 days of the experiment compared to control group. In treatments H50 and H100 blood glucose concentrations increased (P < 0.05) after 30 and 60 days of the experiment, while in treatment H100 NEFA concentration decreased (P = 0.022) after 60 days of the experiment. These findings suggest that hop cones and their constituents provoke changes in energy metabolism in ruminants.
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