Wyniki wyszukiwania - AGRO

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Reactive oxygen species upregulate expression of PAI-1 in endothelial cells

100%

Swiatkowska M., Szemraj J., Al-Nedawi K. N. I., Pawlowska Z.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 4

Second messengers involved in the signal transduction pathway leading to induction of the plasminogen activator inhibitor (PAI-1) have not yet been well characterized. This study focuses on the mechanisms of regulation of PAI-1 expression by reactive oxygen species (ROS) in human endothelial cells. Inhibition of the tumor necrosis factor α (TNFα)-induced expression of PAI-1 by antioxidant N-acetyl-L-cysteine (NAC) indicated redox-sensitive mechanisms involved in the signalling pathway. Because TNFα induces PAI-1 production in endothelial cells, and NAC attenuated this response, we attempted to investigate the possible involvement of ROS in the activation of PAI-1 by TNFα. Upregulation of PAI-1 expression in endothelial cells by the stimulation with TNFα (50ng/ml) or H2O2 (10-200µM), observed by measurement of the antigen and mRNA levels, was reversed in the presence of NAC (20mM). The stimulatory effect of ROS was detected also at the level of the PAI-1 promoter in endothelial cells transfected with plasmid p800 LUC containing a PAI-1 promoter fragment (+71 to -800). The PAI-1 promoter activity was increased in the presence of ROS, and was suppressed by up to 75% in the presence of antioxidants [1], On the basis of this study we can conclude that reactive oxygen species play an important role in a cytokine-induced activation of PAI-1 expression, and may act as a signal transduction messenger.

2

Molecular cloning and characterization of a peroxiredoxin from Phanerochaete chrysosporium

100%

Jiang Q., Yan Y. H., Hu G. K., Zhang Y. Z.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 4

3

ADH expression in aerial-aquatic plants in response to different water environment

88%

Kordyum E., Kozeko L., Ovcharenko Y.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 1 Suppl.

4

A correlation between the expression of nucleolar organizer regions [AgNORs] and some clinico-pathological features of sigmorectal adenocarcinomas

88%

Famulski W., Zalewski B., Sulkowski S., Miller-Famulska D., Sulkowska M.

Folia Histochemica et Cytobiologica

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1999

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tom 37

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nr 2

5

Expression of selected adhesion molecules on peripheral blood leucocytes in patients with aggressive periodontitis

88%

Pietruska M., Zak J., Pietruski J., Wysocka J.

Archivum Immunologiae et Therapiae Experimentalis

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2005

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tom 53

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nr 3

6

Proliferating cell nuclear antigen [PCNA} expression in pituitary adenomas: relationship to the endocrine phenotype of adenoma

88%

Pawlikowski M., Gruszka A., Kurnatowska I., Winczyk K., Kunert-Radek J., Radek A.

Folia Histochemica et Cytobiologica

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2006

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tom 44

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nr 1

7

The expression of Fasciola hepatica antigens in plants

88%

Czaplinska M., Wedrychowicz H., Plucienniczak A., Legocki A. B.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

8

PCNA and p53 expression in relation to clinicopathological features of oral papilloma

88%

Sulkowski S., Famulski W., Kasacka J., Sulkowska M., Klimiuk A.

Folia Histochemica et Cytobiologica. Supplement

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2001

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tom 39

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nr 1

9

Expression of nucleolar organizer regions [NORs] in ovarian epithelial tumors

88%

Terlikowski S., Lenczewski A., Famulski W., Sulkowski S., Kulikowski M.

Folia Histochemica et Cytobiologica

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2001

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tom 39

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nr 2

10

Expression of cyclooxygenase-2 in the inflammatory changed porcine uterus

75%

Jana B., Kozlowska A., Koszykowska M., Majewski M.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 1

In the present study, the pattern of cyclooxygenase-2 (COX-2) expression in health and inflamed porcine uteri was analyzed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry. On day 3 of the estrous cycle, 50 ml of saline or 50 ml of Escherichia coli (E. coli) suspension containing 10⁹ colony-forming units/ml, were injected into each uterine horn of the control (n=6) and experimental gilts (n=7), respectively. This latter procedure lead to a moderately (n=3) or severely intense (n=4) acute endometritis after eight days. Expression of both the COX-2 mRNA and protein was increased in the endometrium (ENDO) of animals suffering from the moderate (P<0.05, P<0.01, respectively) and severe (P<0.01) acute endometritis, as compared to the control tissues. Moreover, COX-2 mRNA level and protein content were higher (P<0.05) in the ENDO of animals with severe than with a moderately acute endometritis. An elevation in the COX-2 gene (P<0.05) and protein (P<0.001) expression was also observed in the myometrium (MYO) of animals suffering from severe endometritis, when compared with the levels observed in MYO of both the health and moderate intensely inflamed uteri. However, both the COX-2 mRNA and protein levels were similar in MYO of the control and moderately inflamed organs. The luminal epithelium, some of uterine glands and circular layer of the MYO were more intensely stained for COX-2 in animals with severe endometritis, than in animals with healthy or moderately inflamed uteri. Nonetheless, stronger COX-2 reaction was found in some of the uterine glands in latter group, when compared to that observed in uteri of the control animals. While positive COX-2-labeling was observed in the muscular layer of all arteries supplying the health and inflamed uteri, such staining was exclusively present in the endothelium of some arteries in inflamed organs. Likewise, some arteries in uteri of the animals with severe endometritis displayed immunoreaction stronger than that found in uteri of the animals with moderate inflammation. The present study revealed an up-regulation of COX-2 mRNA and protein in the inflamed porcine uterus, which was directly related to the intensity of the organ inflammation. An increase in the COX-2 expression in the uterus challenged by E. coli-induced inflammation indicates that this enzyme is crucial for elevated prostaglandins production in the inflamed organ.

11

Bioinformatics analysis and expression of a novel protein ROP48 in Toxoplasma gondii

75%

Zhou J., Wang L., Zho A., Lu G., Li Q., Wang Z., Zhu M., Zhou H., Cong H., He S.

Acta Parasitologica

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2016

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tom 61

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nr 2

12

Edwardsiella ictaluri LuxS: activity, expression, and involvement in pathogenicity

75%

Zhang M., Sun L.

Polish Journal of Microbiology

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2012

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tom 61

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nr 4

Edwardsiella ictaluri is a Gram-negative bacterium and the causative agent of enteric septicemia of catfish. In this study, we examined the expression and function of the LuxS from a pathogenic E. ictaluri strain, J901. J901 was found to produce autoinducer 2 (AI-2) activity that maximized at mid-logarithmic phase and was enhanced by glucose and repressed by high temperature. Consistently, a luxS gene (luxSEi) was identified in J901, whose expression was regulated by cell density, glucose, and temperature in a manner similar to that observed with AI-2 activity. Further analysis showed that LuxSEi is a biologically active AI-2 synthase that was able to complement the luxS-defective phenotype of Escherichia coli DH5. To examine the functional importance of LuxSEi, a genetically modified variant of J901, J901Ri, was constructed, in which luxSEi expression was blocked by RNA interference. Compared to the wild type, J901Ri was (i) reduced in AI-2 activity to a level of 59% of that of the wild type; (ii) impaired in both planktonic and biofilm growth; (iii) significantly attenuated in the ability to infect cultured fish cells and to cause mortality in infected fish; (iv) unable to induce the expression of certain virulence-associated genes. Addition of exogenous AI-2 failed to rescue the growth defect of J901Ri as free-living cells but restored biofilm production and the expression of virulence genes to levels comparable to those of the wild type. Taken together, these results indicate that LuxSEi is a functional AI-2 synthase that is required for optimal cellular growth and host infection.

13

Alcohol dehydrogenase expression in aerial-aquatic plants in response to different water environment

75%

Kozeko L., Ovcharenko Y., Kordyum E.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2008

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tom 524

Alisma plantago-aquatica L. (Alismataceae) and Sium latifolium L. (Apiaceae) can grow both under flooding and moderate water deficit. Activity of alcohol dehydrogenase (ADH) plays a critical role in the metabolism of plants under hypoxia participating in fermentation of sugars to ethanol - the primary mechanism of energy production in hypoxic roots. This study was aimed to characterize localization of ADH activity and its isozyme spectrum in roots of the aerial-aquatic and terrestrial plants. Cytochemical localization revealed that the ADH activity was associated with the root tip in aerial-aquatic plants. The ADH spectrum consisted of two isozymes in the A. plantago-aquatica roots and three isozymes in the S. latifolium ones. At the same time ADH activity was not observed in the roots of terrestrial plants. However, the weak ADH activity appeared in the roots of terrestrial plants after temporary flooding in time-dependent manner. The established changes in ADH gene expression in different water environment demonstrate its significant role in plant phenotypic plasticity.

14

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Effect of short-term fasting on the expression of ACTH (cMC2) receptor in the adrenal glands of chickens (Gallus gallus domesticus)

75%

Szpregiel I., Wronska D.

Roczniki Naukowe Polskiego Towarzystwa Zootechnicznego

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2021

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tom 17

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nr 3

15

Cloning and expression of apyrase gene from Ancylostoma caninum in Escherichia coli

75%

Qiao Y., Pengsakul T.

Acta Parasitologica

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2015

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tom 60

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nr 1

16

Expression of HLA-G in patients with B-cell chronic lymphocytic leukemia [B-CLL]

75%

Giannopoulos K., Dmoszynska A., Bojarska-Junak A., Schmitt M., Rolinski J.

Folia Histochemica et Cytobiologica

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2008

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tom 46

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nr 4

457-460

17

Review of the expression of antimicrobial peptide defensin in honey bees Apis mellifera L.

75%

Ilyasov R. A., Gaifullina L. R., Saltykova E. S., Poskryakov A. V., Nikolenko A. G.

Journal of Apicultural Science

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2012

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tom 56

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nr 1

18

mRNA expression of the cyclin in B-cell

75%

Antosz H., Kowal M., Kwiatkowska-Drabik B., Marzec B., Jargiello M., Mizerski G., Kocki J., Wysocka K.

Bulletin of the Veterinary Institute in Puławy. Supplement

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2002

19

Expression profile analysis of human peripheral blood mononuclear cells in response to aspirin

75%

Choi S., Park H. S., Cheon M. S., Lee K.

Archivum Immunologiae et Therapiae Experimentalis

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2005

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tom 53

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nr 2

20

The delayed early gene G23 of temperate mycobacteriophage L1 regulates the expression of deoxyribonuclease, the product of another delayed early gene of the phage

75%

Mandal P., Datta H. J., Sau S., Mandal N. C.

Polish Journal of Microbiology

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2008

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tom 57

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nr 2

To get clues about the genes as well as the gene regulatory circuit controlling the lytic development of temperate mycobacteriophage L1, previously we screened several conditional lethal mutants of L1 and characterized some of them to an extent. One of the mutants, L1 G23ts23, was found defective in both growth and late gene transcription at 42°C but not at 32°C. Here we show that the above phage mutant is also defective in the expression of phage-coded deoxyribonuclease (DNase) at 42°C but not at 32°C. The G23 gene however does not code for the above enzyme. Further analyses using the L1 G23ts23 mutant suggest that synthesis of DNase is also not regulated by G23 at transcriptional level. Expression of functional DNase in fact requires de novo protein synthesis. Among the 25 revertants isolated from the L1 G23ts23 mutant, which are capable of growing at 42°C (by overcoming the ts defect in late transcription), two, R4 and R22, have been shown to retain the ts defect in the expression of the above enzyme and R4, to retain also the G23ts23 mutation. This suggests that R4 (R22 was not tested for the presence of G23ts23 mutation) carries an extragenic suppressor of G23ts23 mutation in a different gene (we call this putative gene as Gx), which now helps bypass the requirement of G23 for late gene transcription. Possible role of G23 on the regulation of L1-coded Gx and deoxyribonuclease has been discussed at length.

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