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Juniperus communis L. (Cupressaceae) is a plant widely cultivated in the Northern hemisphere. Juniper berries, the fruit of Juniperus communis L. are a highly valued, essential oil-rich plant material used traditionally in folk medicine as antiseptic, diuretic, antirheumatic, anti-inflammatory, antibacterial and antifungicidal agent. This paper reviews information on extraction methods of the essential oil from the juniper berries, its chemical composition and antimicrobial as well as antioxidant properties.
In this study, the antimicrobial properties of essential oils obtained from Coriandrum sativum, Foeniculum vulgare Miller, Salvia triloba, Laurus nobilis L., Citrus limon and Origanum smyrnaeum L. were investigated. A total of eight microbial organisms belonging to six species of bacteria, namely Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter aerogenes, as well as two fungi, Candida albicans and Aspergillus niger, were studied using a disc-diffusion and agar dilution (minimal inhibition concentration) method. The antimicrobial activity of essential oils obtained from the six plants turned out to be more effective in the case of bacteria than against fungi. The antimicrobial activity against Gram-positive bacteria was more pronounced than against Gram-negative ones. All the investigated plants are known as having healing properties and are used to treat various diseases. The essential oils obtained from L. nobilis and O. smyrnaeum showed the highest antifungal activity against C. albicans and A. niger, while the essential oils obtained from F. vulgare showed the highest antimicrobial activity against P. aeruginosa and E. coli. On the other hand, the essential oils obtained from O. smyrnaeum showed stronger antibacterial activity in the case of E. aerogenes and S. aureus, but were not equally effective against E. coli. The other crude essential oils showed varied levels of antibacterial and antifungal activity. The minimal inhibition concentrations (MIC ) of the essential oils obtained from O. smyrnaeum and of those obtained from L. nobilis ranged from 1.17 to 4.71 mg/ml, and 2.4 to 19.2 mg/ml, respectively.
The gas chromatography–mass spectrometry (GC–MS) analysis of essential oil obtained by hydrodistillation from the flower of Taraxacum officinale L. revealed the presence of 25 compounds with 1,3-dimethylbenzene, 1,2-dimethylbenzene, 1-ethyl-3-methylbenzene, heneicosane and tricosane as the main components.
In the framework of the performed studies, the antibacterial activity of the following fungicides was evaluated: Miedzian 50 WG (active substance - a.s. 50% copper oxychloride), Ridomil MZ Gold 68 WG (a.s. 3.8% metalaxyl-M and 64%, mancozeb), Euparen Multi 50 WG (a.s. 50% tolylfluanid), Captan 80 WG [a.s. 80% N-(captan)], Dithane Neotec 75 WG (a.s. 75% mancozeb). The evaluation also concerned the essential oils: lavender, sage, lemon balm, clove, and a preparation based on thyme oil (BioZell). Each preparation and compound was tested against the following bacterial pathogens: Erwinia amylovora, Xanthomonas arboricola pv. corylina, X. arboricola pv. juglandis, Pseudomonas syringae pv. syringae, Agrobacterium tumefaciens (presently Rhizobium radiobacter). Each preparation and compound was tested at a concentration of 1,000 ppm of active substance. Copper oxychloride was also tested at a concentration of 1,500 ppm. Among the tested fungicides, metalaxyl-M with mancozeb, mancozeb alone, and copper oxychloride inhibited all of the tested strains of pathogenic bacteria. Tolylfluanid did not inhibit any of the bacteria used. Out of the investigated essential oils, the strongest inhibitors of bacteria were: sage, cloves, and BioZell. The protective activity of the above mentioned fungicides was also evaluated in vivo. They were assessed against fire blight on apple blossoms and pear fruitlets, against bacterial canker on sweet cherry fruitlets, and against crown gall on sunflower seedlings (the test plant). All fungicides were applied at the same concentrations as those in the in vitro tests. Only copper oxychloride was found to show protective activity against the studied diseases. This result indicates that the antibacterial properties of the other fungicides did not correspond with their activity on the plant organs used in the in vivo experiment.
Eupatorium triplinerve Vahl belongs to the Asteraceae family, popularly known as Ayapana. It is a perennial shrub native to Amazon rainforests of South America. Its leaves are used through infusions, decoctions, baths, and tea. It is largely used in Brazilian folk medicine as sedative, febrifuge, stimulant, tonic and anti-inflammatory. The essential oil from aerial parts of Eupatorium triplinerve was analysis by gas chromatography mass spectroscopy (GC-MS). Thirty compounds representing 98.24 % of the oil were identified. The major compounds were 2-tert-butyl-1,4-methoxybenzene (74.3 %) and b-Selinene (8.6 %). The antibacterial activity of the essential oil was evaluated against ten bacteria (including Gram positive and Gram negative) and six phytopathogenic fungi. The oil exhibited moderated antibacterial and strong antifungal activity against all the test pathogens. The highest zone inhibition was recorded against Salamella typhae (21 mm) followed by Shigella sonnei (18 mm) at a concentration of 20μl/ disc on the other standard Ampicillin 20mg/ disc showed on average of 14-34 mm diameter of zone inhibition against the test organism. The lowest antifungal activities were recorded against Macrophomina phaseoline, and Botryodiplodia theobromae. The highest Alternaria alternate (12000 ppm). Further pharmacological and toxicology studies will be required to establish the therapeutic uses of this oil.
In 2008–2010, in the field experiment, the yield and quality of lavender flowers in organic cultivation were tested. The experiment was established in four different locations in Poland. The following features were evaluated: fresh and dried flower yields, seed yield, weight of 1 000 kernels, essential oil content and its composition and microbiological contamination. Lavender flower yield from organic experiments was higher compared with the yield from conventional cultivation. The content of essential oil in organic lavender flowers was lower then that from conventional ones. The content of essential oil and its composition of lavender flowers did not depend on localization. The satisfied yield of lavender seeds was obtained in organic experiments. The investigated lavender raw materials were below the level of standard contamination for raw materials treated with hot water (A) according to European Pharmacopoeia 7.0.
Background. The copaiba oil is a common natural product used in cosmetic industry and as a nutraceutical product. However, lack of quality control and scarce knowledge about its antimicrobial activity is a point of concern. The proposal of this study was to investigate the physicochemical properties and the antimicrobial activity of five commercial brands of copaiba oil. Material and methods. Acidity and ester index, refractory index, solubility in alcohol, and thin layer chromatography were performed to verify the physicochemical properties of five commercial copaiba oils sold in local pharmacies. Ultra performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-DAD/ESI-Q-TOF-MS) was used to investigate diterpene acids while the volatile compounds were analysed by gas chromatography-mass spectrometry (GC-MS). Antibacterial and antifungal activities were also evaluated by agar diffusion technique; and minimal inhibitory concentration and maximal bactericidal concentration were defined for each sample and bacteria. Results. The physical-chemical analysis revealed heterogeneity between all samples analysed. The A1 sample showed characteristics of copaiba oil and was mainly composed by hydrocarbon sesquiterpenes (29.95% β-bisabolene, 25.65% Z-a-bergamotene and 10.27% β-cariophyllene). Among diterpene acids, the UPLC- DAD/ESI-Q-TOF-MS data are compatible with presence of copalic and/or kolavenic acid (m/z 305 [M + H]+). Candida albicans was sensitive to almost all samples at high concentration and Saccaromyces. cere- visiae showed sensitivity to A1 sample at 100 mg/mL. Although variable, all samples showed antibacterial activity. Significant activity was seen for A3 (19.0 ±0 and 15.6 ±0.5 mm), A4 (16.6 ±0.5 and 15.6 ±0 mm), and A5 (17.1 ±0 and 17.1 ±0 mm) on Staphylococcus saprophyticus and S. aureus, respectively. All samples were active against Klebsiella pneumoniae showing >15 mm diameter halo inhibition; and only A2 was active against Eschirichia coli. Phytopatogens tested revealed resistance of Ralstonia solanacearum CGH12 to all samples and susceptibility of Xcv 112 strain of Xanthomonas campestris pv campestris to almost all samples. MIC and MMC showed bacteriostatic effect against clinical interest bacteria and bactericidal effect against phytopatogens. Conclusion. The results from physicochemical analysis reinforce the fact that it is imperative to include simple conventional methods in the analysis of oil products. The analysis of copaiba oil gives safe products and purity which ensure products with quality. Also, since copaiba oil is an over-the-counter product the results indicate that pharmacosurveillance must be improved by the governmental regulation agency to avoid microorganism resistance selection and to achieve better international quality products.
Nowadays, safety of chemical preservatives has been questioned by a big number of consumers. Traditionally used preservatives often cause skin irritation and lead to allergenic reactions. Growing demands for more natural and preservative-free cosmetics promoted an idea of the replacement of synthetic preservatives with essential oils (EOs) of antimicrobial properties. The antimicrobial effect of essential oil depends on content, concentration and interactions between the main active compounds. Effective preservatives should be characterized by a broad spectrum of antimicrobial activity at a minimum concentration. Formulations containing both types of preservatives: essential oil and a synthetic one have been tested and proposed as a compromise that allows for reducing concentration of both components due to their synergistic activity. Although most essential oils are regarded as safe, some of them may cause risk of contact allergy or phototoxic reaction. A well balanced risk-benefit assessment of essential oils is one of the great challenges for scientists or health policy authorities. This paper presents current state of knowledge on essential oils focused on their antimicrobial properties, the assessment of their efficacy and safety as cosmetic preservatives.
Ziziphora species are traditionally used for treatment of different infectious and non-infectious diseases as antiseptic agents. The aim of this study was to evaluate the chemical composition of Ziziphora clinopodioides and Z. tenuir essential oils and their antifungal effects againt five strains of dermatophytes. GC and GC-MS methods were used for essentional oils analize. The anti-elastase activities were determined by porcine pancreatic elastase assays. 48 different compounds were identified in these two essential oils, which thymol, p-cymene, 1,8-cineole and γ-terpinene were their major components. The anti-dermatophyte activities of essential oils against dermatophytes showed that the essential oils (150 ppm) inhibited the mycelium growth, about 5–100%, which Z. clinopodioides essential oil had higher mycelium growth inhibition (28–100%) than that of Z. tenuir oil. The MIC and MFC values of essential oils were 0.01–1 μl/ml. 0.5 μl/ml essential oils inhibited porcine pancreatic elastase, dose-dependently. Due to the anti-dermatophyte and anti-elastase effects of Ziziphora sp., it can be considered as natural antifungal agent for more clinical and pre-clinical trials.
Drying is the most common and also the simplest method of preserving fresh plant materials. This process greatly extends the life of the product by the removal of water, which decreases the rate of chemical and enzymatic reactions, or even inhibits them. The drying step can cause a change in appearance, taste, color and consistency, as well as reduce the quantity of essential oils, polyphenols, pigments and vitamins. These changes can be significantly reduced by using suitable drying techniques, depending on the material. The choice of the technique should be based on knowledge of the biological, physical and chemical characteristics of the raw material. The paper presents the effects of drying on the quantitative loss of essential oils and compounds with antioxidant properties, as well as changes in the antioxidant properties of selected herbs and spices dried by different methods. Both, the food industry and the cosmetics or pharmaceuticals industry generates a demand for high-quality raw materials, and hence research is being conducted both on preservation methods and on their impact on the quality of the obtained material. It seems that the new technology of drying material in a fluidized bed, greatly reduces or even eliminates the disadvantages of current procedures.
The essential oil of aerial parts of Agastache foeniculum (Lamiaceae) was isolated by hydrodistillation method and constituents of oil were analyzed by gas chromatography mass spectrometry (GC-MS) method. Methyl chavicol, 1,8-cineole, 1-octen-3-ol, 3-octanone and germacrene D the predominant components of oil. Methyl chavicol (94.003%) was identified as a major component in the oil. Essential oil was tested for toxicity against adults of Oryzaephilus surinamensis L. and Lasioderma serricorne F. The influence of different concentrations of the essential oil vapours on adult mortality was significant. Data of probit analysis showed that a lethal concentration of the essential oil to kill 50% of the population (LC50) for adults of O. surinamensis and L. serricorne were 18.781 and 21.565 μl/l respectively. O. surinamensis was more susceptible than L. serricorne at the exposure time 24 h. The results demonstrated that mortality increased with the increase in concentration and exposure time. These results showed that the essential oil from A. foeniculum could be applicable the management of population of stored-product beetle pest.
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