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Various forms of nitrogen fertilizer were applied in the cultivation of red cabbage (in the years 2003–2005), ‘Langendijker’ c.v.: control (unfertilized with nitrogen), Ca(NO3)2 , (NH4)2SO4 , NH4NO3 , CO(NH2)2 applied as solid fertilizers. This experiment aimed at determining the influence of various nitrogen forms on the content of Al, B, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Sr, Ti, Zn, V in cabbage heads and the changes in the content of the available form of these elements for plants in soil after cabbage cultivation. The heads of plants fertilized with calcium nitrate and urea were characterized by the highest content of Al, Mn, Sr, Zn, Cd and Mo. Urea caused a significant increase in the content of Cu, Li and V, while ammonium nitrate resulted in an elevated level of Fe and Co accumulation in cabbage heads. Fertilization with (NH4)2SO4 led to a substantial decrease in the content of Al, Mo and V, and fertilization with NH4NO3 caused a decline in Sr concentrations in cabbage in comparison with other objects of the experiment. Each nitrogen fertilizer resulted in the decreased concentrations of Ti in cabbage heads. Applied nitrogen fertilizers significantly influenced the contents of readily soluble forms of B, Fe and Pb in soil after cabbage cultivation.
The influence of different doses of nitrogen applied in the sewage sludge and mineral fertilizers on the content and uptake of Mo, Fe, B, Mn, Se and Ti by willow (Salix viminalis) was investigated. In the field experiment the willow was cultivated on the objects fertilized with sewage sludge which contained the nitrogen in following doses in kg ha⁻¹: 100, 150 and 200. Also the objects with mineral nitrogen contained 150 kg N ha⁻¹, phosphorus and potassium in doses where the ratio N:P:K was equal 1:0.35:1 was used. The content of Mo and Fe in willow’s branches cultivated on the objects fertilized with sewage sludge was the highest after use of the smallest dose of nitrogen (100 kg N ha⁻¹). The content of Se, Ti and Mn in investigated biomass of willow branches was the highest after use of sewage sludge in the highest dose (200 kg N ha⁻¹). The content of boron marked in studied branches did not depend on applied fertilization. The total uptake of marked vestigial chemical elements by the biomass of willow in period 3 years of experiment was the most often highest on objects fertilized with the highest dose of sewage sludge (200 kg N ha⁻¹). The most of Fe, B and Ti taken willow cultivated in second year, however Mo, Mn and Se in third year of experiment.
Charophytes possess the capacity to store large amounts of various elements, but until today no studies about known methods of determining CaCO₃ and elements in charophytes have been conducted. Because of the large and varying amounts of CaCO₃, it is difficult to estimate the actual element content in dry mass. Simple comparisons of elements in charophyte plant tissue or between charophytes and higher plants are impossible. The main aim of this study was to investigate through laboratory experiments content of carbonates and elements in charophyte tissue using different methods of decomposition (mineralization). The proposed protocol: determination of calcium carbonate content in plants, determination of elements present in the plant dry mass, and calculation of the element content in the plant dry mass as described.
The effects of different types of arbuscular mycorrhizal (AM) fungi on the root inoculation and plant elemental content of lupin (Lupinus albus L.) were investigated in the present study. The growth and development of lupin were examined to determine the species of AM fungi that can help to grow lupin with a high protein content and economic value. In this study, which was carried out as a pot experiment under controlled greenhouse conditions, first the pots were inoculated with Glomus geosporum, Glomus mosseae, Glomus caledonium, Glomus etunicatium mycorrhizal spores and then lupin (Lupinus albus) seeds were sown. The plants were watered with pure water during the experiment. The trial was terminated after a 60-day plant gowing period. In the study, inoculation occurred at lupin roots at rates varying between 13.3 and 30.0%. However, there was no statistically significant difference among the types of arbuscular mycorrhizal fungi applied to the plant in the inoculation rate. The examination of the effect of the application of different AM (Glomus geosporum, Glomus mosseae, Glomus caledonium, Glomus etunicatium) spore on the plant development showed that AM inoculation did not have an effect on the lupin development. The effect of AM inoculation on the plant’s nutrient content revealed no significant difference in the content of crude protein, P and K, while demonstrating a significant increase in the sulphur and magnesium content versus the control. The plant content of crude protein varied between 185.6 and 226.5 (g kg-1), phosphorus – 0.61-0.74 (g kg-1) and potassium – 9.6-11.1 (g kg-1). The concentrations of Zn, Cu, B and Mo in lupin did not show statistically significant modifications caused by the inoculation of different types of AM. However, the plant Mn content showed a decrease due to AM inoculation, whereas a significant increase was observed in the Na content after AM inoculation. AM fungi were observed in plant roots after the inoculation with any of the four different types of mycorrhiza. But no positive effects of mycorrhizal inoculation were not observed on crude protein and the uptake of plant nutrients.
The present study focuses on two aspects of meat quality: the concentration of trace elements and nutritional value. In 2016, total pigmeat slaugtherings in the EU increased by 0.2% despite a significant reduction in the EU sow herd (-228 000 heads) over the last two years. Nevertheless, pigmeat is one of the most consumed meats worldwide, its consumption in the EU countries in 2016 was 32.25 kg/per capita annualy. As one of the most important factors affecting meat quality prior to slaughter is breed, therefore, it is important to evaluate the content of trace elements significant for human health, and meat nutritional value, of various pig breeds. The aim of this study was to describe the nutritional value (dry matter, proteins, intramuscular fat and total minerals) and contents of elements (sodium, magnesium, calcium, zinc, selenium, iron, copper, nickel and barium) in the longissimus dorsi muscle (MLD), obtained from pigs of eight pure breeds and from crossbred pigs, reared under the same conditions. The correlation between tissue composition and element contents was estimated. Element concentrations were assayed by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion. Composition of MLD collected from pigs of various breeds did not considerably differ except for the amount of fat. Meat of pigs of different breeds and their crosses differed in terms of element contents. The highest amounts of Na (P<0.05), Mg (P<0.001) and Ba were recorded in meat of Yorkshire and White Large crossbreeds; those of Ca (P<0.001), Fe (P<0.05) and Ni (P<0.05) were highest in Landrace and White Large crosses; while levels of Zn and Se (P<0.05) were highest in Pietrain pigs, and that of Cu (P<0.01) – in Yorkshire and Pietrain crosses. The correlation of element contents with meat quality indexes in the MLD of the purebred pigs and crosses of these breeds was low or moderate. However, pork nutritional value and element contents were affected by pig breed.
Analyses of three macroelements and six microelements in reed stems, accompanied by investigation of periphyton density were carried out in two reservoirs: Żarnowieckie Lake and Puck Bay. To reveal the influence of chemical composition of biotic substrate on periphyton qualitative and quantitative characteristics, ordination methods were applied (PCA, CCA, DCA, RDA). The results indicated that Copoepoda (Harpacticoida) and Chlorophyta preferred reed substrate with relatively high zinc, sulfur and chromium but low manganese and carbon content. In turn, Nematoda reached the highest density on a substrate rich in manganese. Mercury in reed limited density of Arachnidae-Hydrachnella and Chironomidae larvae, an effect which was not observed for Ciliata libera. Copepoda (Harpacticoida) preferred low chromium and lead content and high carbon/nitrogen ratio, which meant low nitrogen concentration in the reed substrate. Moreover, preferences of Nematoda and Bacillariophyta for freshwaters and Copepoda for brackish waters were indicated. The results presented in this paper should be treated as a contribution to more detailed research on interactions between reed chemical composition and periphyton density.
Concentration of heavy metals and macronutrients were measured in water, bottom sediments and in the leaves of Nymphaea alba and Nuphar lutea sampled from fourteen eutrophic lakes in West Poland. The concentrations of macro- and microelements in examined plants differ significantly and depend on chemical properties of the water and bottom sediments. These dependences are confirmed by calculated significant correlations. Strong positive correlations were found between concentrations of Ba, Co, Mn, and Cu in Nymphaea alba as well as Sr in Nuphar lutea and concentration of this element in environment, indicating the potential of examined plants for monitoring for these metals.
Modern forestry management should be based on the principle of sustainability.In order to preserve habitat productivity, the amount of nutrients removed from the environment along with forestry products must be taken into consideration. This study shows the exact concentrations of chemical elements in different tree parts of Scots pine, growing on poor soils in north-western Poland. The observedvalues were compared to the values found in literature. In addition, the relationship between the concentrations of elements and stem diameter or stand density was researched. The highest concentration of chemical elements was observed in the needles (C, N, P, K, Mg, S, Mn, Na, Fe) and the lowest (C, N, P, S, Cu, Na, Ni, Pb, Zn, Fe) in the stem wood. Most of the macronutrients (P, K, Ca, Mg and S) reached optimal values, with the exception of N showing a deficiency, especially in the needles. The relationship between the content of elements and DBH or stand density was rather weak, and in both cases, negative.
W celu oceny wpływu masowych grobów na zawartość pierwiastków przeprowadzono badania na terasie plejstoceńskiej Wisły w Puszczy Niepołomickiej w okolicach Niepołomic niedaleko Krakowa. Badania te prowadzono w środowisku okresowo podmokłego piasku z domieszkąminerałów ilastych w górnej części pionowego profi lu. W środowisku tym stwierdzono istotny wpływ masowego grobu na: podłoże, mech płonnik, żywicę sosny, hubę brzozy oraz powietrze. Wielkości zmian oceniono na podstawie różnic w koncentracji pierwiastków w środowisku grobu względem miejsc porównawczych. W otoczeniu badanego grobu wykazano stosunkowo duże zawartości pierwiastków pochodzących ze zwłok ludzkich: P, S, N, C, Cl, Na oraz Ca. Szczególnie charakterystyczna jest wyższa zawartość P w podłożu oraz w powietrzu. Natomiast w hubie brzozy potwierdzono relatywnie wyższą koncentrację pierwiastków ciężkich.
Foliar application of growth regulators or fertilizers containing biostimulators can influence the uptake and accumulation of mineral elements by plants. The aim of the research was to determine the influence of foliar application of ‘Pentakeep V’ and diverse nitrogen fertilization on the content of: Ag, Al, Ba, Cd, Co, Cr, Ga, Li, Ni, Pb, Sb, Sr, Ti and V in spinach leaves. In 2006–2007 pot experiment with spinach Spinacia oleracea L. ‘Spinaker F1’ cultivation on silty medium loam was carried out. The experiment design included two sub-blocks: with and without foliar nutrition. Plants with foliar nutrition were sprayed twice with 0.02% w/v ‘Pentakeep V’ fertilizer (3000 dm3 water per 1 ha). In each sub-block soil nitrogen fertilization (in the form of ammonium nitrate) was applied in following combinations: 1 – control (without N), 2 – 50% N dose prior to seed sowing (25 mg N∙dm-3 of soil), 3 – 100% N dose prior to seed sowing (50 mg N∙dm-3 of soil). Foliar application of ‘Pentakeep V’ resulted in (compared to not treated plants): a) significant reduction of the Ag, Al, Ba, Ga, Sr and Ti concentration in plants not fertilized with nitrogen, b) increase in Sr content in spinach fertilized with 100% of N dose, c) relatively weak tendency to lower V accumulation in control plants. The lowest concentration of cobalt was found in plants fertilized with 50% of N dose and not treated with ‘Pentakeep V’. No interaction between foliar nutrition and nitrogen fertilization was found in reference to Cd, Cr, Li, Ni, Pb and Sb content in spinach plants.
The study was conducted on 300 turkey cocks type BUT-9, grown under optimal conditions for this kind of poultry. The feed was a typical commercial full-dose mix for turkeys. The birds were divided into 5 experimental groups. From the 3rd week of their life, they were given preparations with their drinking water. Group I was the control group, while drinking water for the test groups was enriched as follows: lactic acid (0.4%) for group II, CuSO4 in the amount of 30 mg Cu·dm-3 H2O for group III, CuSO4 (30 mg Cu-dm-3) and 0.4% of lactic acid for group IV and CuSO4 in the amount of 50 mg Cu·dm-3 for group V. Application of the preparations was terminated after 3 days, when symptoms of poisoning were observed in turkey cocks of groups II and IV, alongside increased mortality rate. Samples of drinking water were taken for analyses, and from each group 10 birds were selected for slaughter to take tissue samples (breast and leg muscles). The objective of the experiments was to determine the concentration of Ca, Mg, Zn, Cu and Fe in the consumable tissues of turkey cocks. The study showed that the highest rate of mortality of turkey cocks was observed in the group that was given lactic acid with their drinking water, and these turkeys had increased levels of Ca, Cu, Zn and Fe. In none of the groups, the content of Zn in the breast muscle exceeded 20 mg kg-1, while the concentration of copper in all experimental groups (except the control) was above the level of 10 mg kg-1. Increased doses of copper caused an increase in the concentration of magnesium in the leg muscles, while a combination of copper supplementation with lactic acid resulted in increased accumulation of Cu in both the breast and leg muscles.
Samples of soil and meadow sward were collected in the first half of June 1992 at 47 spots situated in various directions and distances from the Ironworks (fig. 1). The following directions were taken into consideration: north-west (N-W), north (N), north-east (N-E), east (E), south-east (S-E), south (S) and south-west (S-W). Depending on the direction, the longest distance from the source of emission was from 6 to 19 km. One sample of meadow sward and three samples of soil, one from each of the following horizons: 0-10 cm, 10-20 cm and 30-50 cm were collected. Contents of Cd, Cu, Ni, Pb and Zn were assessed in soil and plant samples. Apart from heavy metals also basic soil properties were assayed in soil samples. Estimation of heavy metals content points to processes of heavy metals cumulation in soil which are taking place in the area around the Ironworks. It concerns particularly zinc and, to a lesser extent, lead and cadmium (tables I and 3). The heaviest pollution of soil with zinc was observed in the areas adjoining the Ironworks from N-E direction, with cadmium from E direction and with lead from E and N directions (Fig 2-6). As regards the vertical distribution, two groups may be distinguished within the investigated heavy metals: one comprises copper and nickel which do not show a vertical variation, the second one includes cadmium, lead and zinc, and their content generally decreases markedly along with depth (tab. 1 and fig. 2-6). Only in case of nickel and zinc their content in soil is positively correlated with die content of heavy metals assessed in sward (tab. 5) Despite of a high content of some metals in the investigated soils — their content assayed in sward is within norm and does not disqualify the hay as fodder (tab. 4).
Industrial landfills resulting from operations of various industries, particularly power generation, create specific habitat conditions for flora. Among the waste which may be used for recultivation of dumping heaps there is sewage sludge, which contains a considerable amount of nutrients crucial for plants. This investigation aimed at identification of the effect of sewage sludge and furnace ashes on the content of selected elements in a mixture of grass and white clover recommended for furnace ash heap recultivation. The experimental design comprised 6 treatments (each in four replications), which differed in a dose of the supplied sewage sludge and furnace ashes: 1) control (without waste admixture), 2) 200 t d.m. of sewage sludge, 3) 200 t d.m. of ash, 4) 150 t d.m. of sludge + 50 t d.m. of ash, 5) 50 t d.m. of ash + 150 t d.m. of ash, 6) 100 t d.m. of ash + 100 t d.m. of ash. The content of macroelements in plants depended on the treatment and ranged from 2.58-31.2 g Mg, 3.16-5.85 g Ca, 16.95-18.46 g K, 0.26-1.25 g Na and 2.27-3.37 g P kg–1d.m. Plants grown exclusively on furnace ashes had the highest content of Mg, Ca and K, whereas the highest P and Na concentrations were noted in plants cultivated exclusively on sewage sludge. While assessing the content of macroelements in the plant mixture in view of its fodder value, it was found that the content of Mg and K met the standards set for good quality feeds, the amounts of Ca and Na were below the optimum, whereas the P concentration was close to the optimum value.
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