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An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing ability was estimated. Based upon their N-fixing ability and IAA production, two strains, one Azospirillum sp. and one Pseudomonas sp. isolate was selected and tested for its performance in brinjal. The seeds treated with 75% Chemical fertilizer + Azospirillum brasilense + Pseudomonas fluorescens (T6) showed maximum plant vegetative characters, followed by others compared with control.
Eggplant fruits are abundant in potassium, the amount of which ranges from 200 to 600 mg K⋅100 g-1 FM, depending on a variety. They are also a rich source of phosphorus, magnesium, calcium, and iron. As there are no fertilization recommendations for eggplant cultivation under cover, this study been undertaken to evaluate the vegetable’s requirements. The aim was to test how the type and dose of potassium fertilizer influences nitrogen, phosphorus, calcium, and magnesium levels in eggplant fruits. The experiment on cv. Epic F1 eggplant was carried out in unheated polyethylene tunnel in 2004-2005. The eggplant was cultivated on peat subsoil in 10 dm3 capacity cylinders made of rigid plastic. The experiment was set up in a two-factor, completely randomized design. The influence of two factors was examined: I – type of potassium fertilizer (KCl, K2SO4, KNO3), and II – potassium rate (8, 16, 24 g K⋅plant-1). Fruit samples for laboratory determinations were collected in mid-August, in the middle of fruiting stage. Fruits were harvested at the stage of technological maturity and the following were determined: Ntot, P, K, Ca, Mg. The results were processed by variance analysis. Significantly higher total nitrogen and potassium concentrations in fruits of plants fertilized with potassium nitrate as compared to the other two fertilizer types were recorded. Increasing potassium doses, regardless the fertilizer type, considerably increased the element content in eggplant fruits and widened the K:Ca ratio value. The diversification of potassium fertilization did not have significant influence on phosphorus and magnesium concentrations in eggplant fruits. No significant changes in calcium content in fruits were observed when applying potassium sulfate or nitrate, while higher potassium chloride rates significantly decreased the concentration of this element in fruits.
The present investigation was carried out to evaluate the potential of chitosan alone and in combination with various agricultural wastes for the management of rootknot nematode, Meloidogyne incognita on eggplant cv. ‘BR-112’ under greenhouse conditions. The results showed that chitosan as a single or joint treatment with agricultural wastes significantly (p ≤ 0.05) reduced root-knot indices, and the nematode population in soil. As a result, of this, the growth and growth yielding attributes of eggplant were remarkably augmented. Chitosan as an elicitor induced plant mediated systemic resistance against M. incognita in eggplant. The results of the study demonstrated that maximum reduction in eggmass/root, eggs/eggmasses, nematode population and root-knot indices, was acquired by the treatments: chitosan + onion and chitosan + mentha. It was followed by chitosan + Brassica, chitosan + urad and chitosan + coconut whereas, chitosan combined with corn cob waste was found to be the least effective when compared to the control. The application of chitosan alone was effective but not very satisfactory. Compared to the control applications of all the treatments significantly increased plant growth in terms of length, fresh and dry weights, pollen fertility, yield and biochemical parameters such as chlorophyll, carotenoid content and antioxidant enzymes. This may have been due to the eliciting activity of chitosan, causing systemic resistance in the plant and the release of various toxic chemical compounds during decomposition which have lethal effects against the second stage juveniles of M. incognita and nematode multiplication.
Brinjal (Solanum melongena L.) suffers from the attack of various pests throughout its different stages of the crop growth. Different insecticides are used to control these pest infestations. The application of these insecticides on brinjal plants may have an effect on their quality and nutritional parameters of fruit samples. In the present study effect of three different insecticides viz. Triazophos 40% E.C. Carbaryl 50% W.D.P, Cypermethrin 10% E.C. and Azadiractin 10,000 ppm formulation belonging to different groups namely organophosphate, carbamate, synthetic pyrethroids and botanical insecticide on brinjal fruit quality, nutritional parameters and total antioxidant capacity (TAC) were assessed in field conditions. The results showed that different insecticides at field application dose showed minute insignificant variation in fruit nutritional and total antioxidant activity parameters in comparison to the control samples. The fruit quality parameters also showed minute variation in comparison to the control, except in the case of Triazophos treated samples which showed significant variation (p<0.05) in total soluble sugar content and crude protein.
In the years 2002–2003, in a foil tunnel, vegetation experiment in the growing of two eggplant cultivars ‘Epic F1’ and ‘Solara F1’ were carried out. Plants were grown in cylinders of 6 dm3 capacity filled with substrate which consisted of: 1) raised peat (from Lithuania), 2) pine bark + low-moor peat (v : v = 1 : 1). In the vegetation period, top-dressing with nitrogen, phosphorus and potassium was applied. Fruit harvest was carried out many times. The total fruit yield was determined. Index parts of plants were sampled for analyses in which the following values were identified: in ‘Epic’ cultivars: 1.12–3.40% N; 0.42–1.14% P; 1.80–4.81% K. In ‘Solara’ cultivars, the following values were found: 1.17–3.50% N; 0.53–1.27% P; 1.96–4.00% K, depending on the substrate and the fertilization level. Differences were found in the total yield and in the nutritional status of plants, depending on the substrate, fertilization level, cultivar and term of sampling.
The paper presents literature data on the flowering biology of eggplant and the influence of flower heterostyly on yielding. Flowers with long–styled pistol are the majority among all flowers on a plant (60%). Number of flowers with medium and short style is lower (10–15% and 22–30%, respectively). Eggplants set fruits from flowers with long style in 49–100% and with medium style in 46–85%. These flower’s stigmata have well – developed nodules and well – permissible tissues rich in polysaccharides, proteins styled flowers have small stigmata with underdeveloped nodules and, due to worse absorption, they are not pollinated and do not set fruits. Taking into account the complexity of many factors that make difficult or impossible to self-pollinate the eggplant’s flowers, the efficiency of three procedures intensifying the fruit budding has been presented: bunch vibrating, flower harmonization, and natural pollinating by bumblebees. Many authors’ studies confirm that flower bunch vibrating has not caused sufficient pollen setting on stomata and has not been sufficient for good eggplant flower pollination. Applying growth regulators appears to be more efficient and significantly affects the improvement of eggplant’s yielding. The highest efficiency has been achieved when applying insects as natural pollinators at cultivating the eggplant under covers.
Due to strong eggplant growth and the formation of large vegetative weight quantities in growing under glass and foil it was deemed useful to conduct studies on the effect of pruning intensity on yielding of this vegetable in rooms. The aim of the foregoing studies was to demonstrate the effect of plant pruning and topping manner on yield quantity and earliness. The experiment was conducted in the years 2001–2003. The study objects were the plants of ‘Epic F1’ cultivar. Eggplant was grown in rigid foil cylinders of the capacity of 10 dm3 in the peat substrates. The plants were trimmed, managing for one, two, three, four, five, six guiding shoots and in the natural form, without trimming. Topping cut was performed in each combination after the first fruit harvest. The experiment was established and conducted as a two-factor one, in accordance with a complete randomization system: A – pruning method (a = 7), B – topped and non-topped plants (b = 2). Each combination of the examined factors was represented by 20 plants (experimental units). A significant effect of pruning intensity on the quantity and earliness yielding was demonstrated. The highest marketable fruit yield was obtained from plants managed for two (3.82 kg.m-2), three (3.98 kg.m-2), and four (3.87 kg.m-2) guiding shoots. Managing for one guiding shoot significantly decreased the total and marketable fruit number. The highest early yield was collected from plants managed for one and two guiding shoots. A single topping cut performed after the first fruit harvest did not affect marketable yield and marketable fruit number.
Eggplant (Solanum melongena L.) is one of the most popular common major vegetable crops worldwide. This study evaluated the nutritional content of seven commercial eggplant fruits in terms of fatty acid, mineral, sugar, organic acid, amino acid and polyamine contents. The most abundant fatty acid was linoleic acid (range, 39.14-53.81%, ave. 45%), and the most abundant mineral was K (range, 1556.2-3171.6 mg/kg fw, ave. 2331.9). The major organic acid was malic acid (range, 129.87-387.01 mg/g fw, ave. 157.49), and the major sugar was fructose (range, 1242.811379.77 mg/100 g fw, ave. 1350.88). The major polyamine was putrescine (11.54 and 25.70 nmol/g fw, ave. 17.86), and the major amino acid was glutamine (148.4 and 298.75 mg/100 g fw, ave. 219.74). Overall, taking into account the export potential of eggplants, these results may contribute to further studies aiming to improve other nutrient-rich varieties of eggplant in breeding programs.
A membrane-bound UDP-glucose:sterol glucosyltransferase from Solanum melongena (eggplant) leaves was partially purified and its specificity as well as molecular and kinetic properties were defined. Among a wide spectrum of 3-OH steroids (i.e. typical plant sterols, androstane, pregnane and cholestane derivatives, steroidal alkaloids and sapogenins) and triterpenic alcohols, the highest activity was found with 22-oxycholesterol. UDP-glucose appeared to be the best sugar donor. The enzyme preparation was also able to utilize UDP-galactose, TDP-glucose and CDP-glucose as a sugar source for sterol glucosylation, however, at distinctly lower rates. The investigated glucosyltrasferase was stimulated by 2-mercaptoethanol, Triton X-100 and negatively charged phospholipids, and inhibited in the presence of UDP, mono-, di- and triacylglycerols, divalent cations such as Zn 2+, Co 2+, high ionic strength, cholesteryl glucoside, galactoside and xyloside and some amino acid-modifying reagents (SITS, DIDS, PLP, DEPC, pCMBS, NEM, WRK and HNB). Our results suggest that unmodified residues of lysine, tryptophan, cysteine, histidine and dicarboxylic amino acids are essential for full enzymatic activity and indicate that a glutamic (or aspartic) acid residue is necessary for the binding of sugar donor, i.e. UDP-glucose in the active site of the GT-ase while histidine and cysteine residues are both important for the binding of the nucleotide-sugar as well as of the steroidal aglycone.
The experiment was carried out in a three unheated plastic tunnels in 19982000. The aim of this study was to estimate the effects of flower' s heterostyly and two methods of flower pollination (self-pollination and using bumble-bee) as well as flower hormonization on the flowering and fruiting of three varieties of aubergine 'Black Beauty', 'Solara F1 and 'Epic F1" The analysis of results showed that the "eggplants formed more flowers in object with self-pollination and flower hormonization than those pollinated by bumble-bee. Regardless of the pollination way and flower hormonization, eggplants formed the highest number of flowers with long pistil and much less - with medium and short pistil. It was shown that the tendency to formation the flowers with particular type of pistil is the variety trait of eggplants. The highest number of flowers with long pistil was observed in varieties 'Solara F1' and Epic F1, and those with medium pistil - in 'Black Beauty' variety. Heterostyly phenomenon occurring in eggplant' s flowers affected the plant's fruiting. The most fruits were set from flowers with long pistils than from those with 'um and short ones. Fruits formed from long pistil flowers were characterized with significantly greater mean weight and size, than those formed from medium and short pistil ones. No significant influence of pollination method on eggplant fruit quality was ,found in three years of study. Fruits achieved due to three pollination methods were characterized with similar mean weight and diameter. Fruits with significantly larger length were achieved from flowers pollinated by bumble-bees than from self pollinated ones.
The aim of the investigations was to determine the regularity of pollination and fertilization as dependent on the phenotype of fl ower in eggplant. The experiment was conducted in 2005 and 2006 at the Agricultural University in Kraków, Poland. The object of the investigations was eggplant (Solanum melongena L.) ‘Epic F1’, grown in the open field. Flower phenotype (long-, medium- or short-styled), fruit setting and the number of seeds per fruit were recorded on experimental plants during the flowering period. The number of pollen tubes in the middle of the style and the number on fertilized ovules in ovaries were evaluated for all types of flowers. The course of pollination was different in particular types of eggplant flowers. Differences concerned only the number of pollen tubes and fertilized ovules of short, medium- and long-styled flowers and the number of seeds in the fruit. There was not observed any incompatibility in the growth on pollen tubes in the styles of all types of eggplant flowers. Stereomicroscopy observations of cross sections of the ovaries do not show differences in the formation and position of the ovules on the placenta. Short-styled flowers were characterized by signifi cantly lower pollination and fruit set effi ciency and they produced fruits with a signifi cantly lower number of seeds. The low number of pollen tubes, fertilized ovules and seeds can be a straight consequence of the morphology of short-styled fl owers: small-sized stigmas and spatial separation of anther pores and stigmas, which makes pollination diffi cult.
A membrane-bound phospholipid:steryl glucoside acyltransferase from Solanum melongena leaves was partially purified and its specificity and molecular as well as kinetic properties were defined. Among the steryl glycosides tested (e.g. typical plant steryl glucosides, steryl galactosides and cholesteryl xyloside) the highest activity was found with cholesteryl glucoside, but some structurally related compounds such as sito- and stigmasteryl glucoside or galactoside as well as cholesteryl galactoside were also acylated, albeit at lower rates. The investigated enzyme was able to use all classes of phosphoglycerolipids (phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol) as an acyl source for biosynthesis of acylated steryl glucoside. Among them 1,2-dimirystoylphosphatidylic acid appeared to be the best acyl donor. Apart from phosphoglycerolipids, 1,2-diacylglycerols were also used as acyl donor for steryl glucoside acylation, although at a distinctly lower rate. The acyl moiety was transferred from the C-1 position of phospholipid molecule. The investigated acyltrasferase activity was stimulated by 2-mercaptoethanol, Triton X-100, 1-monoacylglycerols and inhibited in the presence of divalent cations such as Ca 2+, Mn 2+, Zn 2+ or Co 2+, some lipids (MDGD, ceramide), detergents (Tween 20, 40, 60 and 80, Tyloxapol, sodium deoxycholate) and high ionic strength.
Eggplant fruits are known for being low in calories but rich in minerals, which is good for human health. They are rich in potassium, whose content ranges from 200 to 600 mg K⋅100 g-1 of fresh mass, depending on a cultivar. Eggplant fruits are also a source of magnesium, calcium and iron. Research on the agro-techniques of eggplant culture in a plastic tunnel has implicated that, on account of a very intensive growth of the plant, both plant pruning and training have a decisive influence on the final amount of fresh mass. Since we lack information concerning the fertilization recommendation for growing eggplants under a plastic tunnel, a study has been undertaken to specify such nutritional needs of this vegetable. The aim of this work has been to determine the influence of nitrogen forms and plant training methods on the content of nitrogen, phosphorus, potassium, calcium and magnesium in eggplant fruits. The experiment on cv. Epic F1 eggplant was carried out in years 2004-2005, with eggplants growing in an unheated plastic tunnel. The eggplants were cultivated in cylinder plastic wraps of 10 dm3 volume, in peat. The experiment was carried out in two stages, in a completely random design, with each stage examining different factors. The following factors were examined: I – nitrogen forms: NH4 + (ammonium sulphate – (NH4)2SO4 (20,5% N); NO3 - (calcium nitrate – Ca(NO3)2 (15,5% N); NH2 (urea – CO(NH2)2 (46% N), II – plant training method: natural form of the plant, 3 shoots. Nitrogen was used in the amount of 10 g N⋅plant-1. Samples of fruits used for further laboratory tests were collected in the 2nd decade of August, in the middle of fructification. The fruits were harvested at the marketable stage. N-total, P, K, Ca, Mg were determined in the fruits. The results were elaborated statistically using analysis of variance. Generally, considerably higher content of nitrogen was determined in eggplant fruits fertilised with the N ammonium form; also the content of potassium and magnesium was much higher in comparison to the other nitrogen forms examined. Moreover, significant influence of the plant pruning method on the content of the elements was found, independently of the applied nitrogen fertilization.
The state of knowledge about eggplant and the methods of its storage is the subject of the paper. In the experimental part, the effect of freezing treatment on eggplant fruits was investigated and the range of its cryoscopic temperature was determined.
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