Wyniki wyszukiwania

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miR-375 induces human decidua basalis-derived stromal cells to become insulin-producing cells

100%

Shaer A., Azarpira N., Vahdati A., Karimi M. H., Shariati M.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 3

This paper focuses on the development of renewable sources of isletreplacement tissue for the treatment of type I diabetes mellitus. Placental tissue-derived mesenchymal stem cells (MSCs) are a promising source for regenerative medicine due to their plasticity and easy availability. They have the potential to differentiate into insulin-producing cells. miR-375 is a micro RNA that is expressed in the pancreas and involved in islet development. Human placental decidua basalis MSCs (PDB-MSCs) were cultured from full-term human placenta. The immunophenotype of the isolated cells was checked for CD90, CD105, CD44, CD133 and CD34 markers. The MSCs (P3) were chemically transfected with hsa-miR-375. Total RNA was extracted 4 and 6 days after transfection. The expressions of insulin, NGN3, GLUT2, PAX4, PAX6, KIR6.2, NKX6.1, PDX1, and glucagon genes were evaluated using real-time qPCR. On day 6, we tested the potency of the clusters in response to the high glucose challenge and assessed the presence of insulin and NGN3 proteins via immunocytochemistry. Flow cytometry analysis confirmed that more than 90% of the cells were positive for CD90, CD105 and CD44 and negative for CD133 and CD34. Morphological changes were followed from day 2. Cell clusters formed during day 6. Insulin-producing clusters showed a deep red color with DTZ. The expression of pancreatic-specific transcription factors increased remarkably during the four days after transfection and significantly increased on day 7. The clusters were positive for insulin and NGN3 proteins, and C-peptide and insulin secretion increased in response to changes in the glucose concentration (2.8 mM and 16.7 mM). In conclusion, the MSCs could be programmed into functional insulin-producing cells by transfection of miR-375.

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MAVS is not a likely susceptibility locus for Addison’s disease and type 1 diabetes

86%

Zurawek M., Fichna M., Kazimierska M., Fichna P., Dzikiewicz-Krawczyk A., Przybylski G., Ruchala M., Nowak J.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 3

3

Placental vascular endothelial growth factor expression in pregnancies complicated by type 1 diabetes

86%

Iciek R., Wender-Ozegowska E., Mikolajczak P., Seremak-Mrozikiewicz A., Zawiejska A., Mrozikiewicz P. M., Drews K., Brazert J.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 4

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Relationship with peers of young school-aged children with type 1 diabetes

86%

Ledwon E., Szlenk-Czyczerska E., Gawlik M.

Medical Science Pulse

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2018

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tom 12

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nr 2

Background: Functioning in a peer group plays an important role in child development. Building self-esteem of children depends on many factors such as physical health, self-evaluation of their physical performance, academic achievement, social support, family relationships, as well as relationships with peers and teachers. Importance of contacts with peers increases during school-age. At this time children are not able to reject the opinion of other people and subject their behaviour completely to the expectations of their peers. When the need for emotional contact is not satisfied, the complex of being different appears. Isolation within group causes a sense of inferiority, the child becomes passive and resigned. Children, who are chronically ill, rejected or isolated often follow negative emotions in their actions, they become aggressive towards other children or do not want to continue learning. Often the decision about an individual teaching plan results in further isolation of the child. The child’s position in the peer group affects the developing personality, self-confidence and self-esteem. Aim of the study: The aim of the study was to determine the interpersonal relationships of young school-aged children with type 1 diabetes in their peer groups. Material and methods: The study was conducted on children with type 1 diabetes and their parents, who are supervised by Clinic of Diabetes, Regional Medical Center in Opole. The research tool used in both groups was a questionnaire consisting of open and closed questions elaborated by the authors of this study. Results: 73% (41) of evaluated children declared that diabetes does not hinder their performance at school. 93% (55) children claimed that they do not hide their condition from their peers. In addition, 61% (34) talk about their illness with their peers and 69% (38) of them can count on their peers’ help in self-control of diabetes. 71.9% (46) of parents of children with type 1 diabetes allow their children to participate in school trips and 68.8% (44) to participate in school competitions. Conclusions: In most children, diabetes does not affect children’s performance at school. They participate in classes, are accepted and liked in their peer group. Chronically ill children should be able to participate in all types of activities as much as their healthy peers. It seems necessary to continue studies on performance of young school-aged children with type 1 diabetes in their peer group.

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Polymorphisms of the toll-like receptor-3 gene in autoimmune adrenal failure and type 1 diabetes in Polish patients

86%

Fichna M., Zurawek M., Fichna P., Januszkiewicz-Lewandowska D., Ruchala M., Nowak J.

Archivum Immunologiae et Therapiae Experimentalis

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2016

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tom 64

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nr 1

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Low placental visfatin expression is related to impaired glycaemic control and fetal macrosomia in pregnancies complicated by type 1 diabetes

72%

Iciek R., Brazert M., Wender-Ozegowska E., Pietryga M., Brazert J.

Journal of Physiology and Pharmacology

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2018

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tom 69

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nr 1

7

In vitro generation of pancreatic beta-cells for diabetes treatment. I. Beta-like cells derived from human pluripotent stem cells

72%

Cierpka-Kmiec K., Wronska A., Kmiec Z.

Folia Histochemica et Cytobiologica

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2019

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tom 57

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nr 1

Ograniczanie wyników

miR-375 induces human decidua basalis-derived stromal cells to become insulin-producing cells

MAVS is not a likely susceptibility locus for Addison’s disease and type 1 diabetes

Placental vascular endothelial growth factor expression in pregnancies complicated by type 1 diabetes

Relationship with peers of young school-aged children with type 1 diabetes

Polymorphisms of the toll-like receptor-3 gene in autoimmune adrenal failure and type 1 diabetes in Polish patients

Low placental visfatin expression is related to impaired glycaemic control and fetal macrosomia in pregnancies complicated by type 1 diabetes

In vitro generation of pancreatic beta-cells for diabetes treatment. I. Beta-like cells derived from human pluripotent stem cells