Wyniki wyszukiwania

1

Influence of Fusarium culmorum infection and its metabolite deoxynivalenol on membranes stability in barley seedlings

100%

Wojciechowski S., Wisniewska H., Chelkowski J.

Acta Physiologiae Plantarum

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1996

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tom 18

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nr 1

2

Effect of deoxynivalenol and degradation enzyme of growth performance and immune responses in mule ducks

88%

Cheng Y. H., Chang M. H., Lin Y. A., Wu J. F., Chen B. J.

Journal of Animal and Feed Sciences

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2004

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tom 13

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nr 2

275-287

3

Influence of low doses of deoxynivalenol applied per os on chosen indexes of immune response in swine

75%

Zielonka L., Gajecki M., Obremski K., Zwierzchowski W.

Polish Journal of Veterinary Sciences

|

2003

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tom 06

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nr 3 Suppl.

4

The rapid acoustic screening of deoxynivalenol [DON] in grain

75%

Juodeikiene G., Vidmantiene D., Basinskiene L., Bartkiene E., De Koe W. J.

Ekologia i Technika

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2005

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tom 13

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nr 5

5

Influence of deoxynivalenol on elektrolyte leakage in cereal seedling leaves

75%

Wojciechowski S., Chelkowski J., Kostecki M.

Acta Physiologiae Plantarum

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1995

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tom 17

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nr 4

6

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Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays

63%

Chehri K., Godini R.

Journal of Plant Protection Research

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2017

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tom 57

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nr 3

In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members of FGSC was confirmed molecularly using Fg16F/Fg16R primers. Fusarium asiaticum isolates (4) were distinguished from other FGSC using Fg6CTPSf177/Fg16R primers. Polymerase chain reaction-based (PCRbased) detection of mycotoxin-synthesis-pathway gene was also used to determine the potential of the analysed strains to produce deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-AcDON), 3-acetyldeoxynivalenol (3-AcDON), and nivalenol (NIV). Of 14 tested isolates, 10 and 4 isolates belonged to DON and NIV chemotype, respectively. Also, the results of DON chemotype survey using specific primers MinusTri7F/R and Tri315F/R showed 1 and 9 isolates produced 3-AcDON and 15-AcDON, respectively. These results show that DON was the most common chemotype in western Iran. To our knowledge, this is the first report on 15-AcDON, 3-AcDON, and NIV isolated from barley in Iran.

7

The biotransformation of chosen mycotoxins

63%

Gajecka M., Jakimiuk E., Zielonka L., Obremski K., Gajecki M.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 2

293-303

Despite the unfavourable influence of mycotoxins on human and animal health and few toxi- cological aspects that have been documented, about these biologically active substances has not been explored. Aiming at more knowledge and a better understanding of the effects and mechanism of mycotoxin action in mammals would provide the basics for developing strategies to restrain different mycotoxicoses. One of the processes not fully understood is biotransformation, to which mycotoxins are subjected the animal organism. Biotransformation is the conversion of mycotoxins to non-toxic metabolites and occurs mostly in the intestinal mucosal membrane and liver, although other tissues and systems also take part in this process. Mycotoxin biotransformation reactions can be considered bioinactivation or detoxication, but mycotoxin biotransformation processes could also result in products more toxic than the mycotoxin. It can be concluded from research studies that our knowledge of mycotoxin biotransformation is scarce.

8

A survey of deoxynivalenol and zearalenone content in commercial dry foods for growing dogs

63%

Holda K., Glogowski R.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2014

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tom 53

A survey of Deoxynivalenol and Zearalenone content in commercial dry foods for growing dogs. The Polish market of commercial dry dog food for growing dogs of small breeds was surveyed for the presence of DON and ZEN with its metabolites α- and β-zearalenol (α-, β-ZOL). LC/ /MS method was applied for 6 randomly selected foods. The low levels of toxins found, gives overall picture of the safe segment of the market. The content of masked α-zearalenol, however, from the perspective of long term consumption of small amounts may pose a health risk for the reproductive system of bitches.

9

Fusarium mycotoxins in Lithuanian cereals from the 2004-2005 harvests

63%

Mankeviciene A., Butkute B., Dabkevicius Z., Suproniene S.

Annals of Agricultural and Environmental Medicine

|

2007

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tom 14

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nr 1

Fusarium mycotoxins deoxynivalenol (DON), T-2 toxin, and zearalenone (ZEN) contamination in 5 kinds of cereal grain harvested in 2004 and 2005 in different regions of Lithuania was examined for their occurrence frequency and level. In all cereal species DON was the most frequently detected mycotoxin with an incidence rate of 98.0 - 100% and range in positive samples from traces to 691 μg kg-1 in 2004 and 62.5-94.0%, range from traces to 1,121 μg kg-1 in 2005, respectively. All the tested oat samples collected in 2004-2005 were found to be contaminated with the T-2 toxin. In one sample from the year 2004 the level of T-2 toxin (121.5 μg kg-1) exceeded the allowable level. In 2004, ZEN contamination was more frequent in spring wheat, barley and oats grain, whereas in 2005 this toxin was identifi ed at higher levels only in barley grain (68.0%). In one barley grain sample from 2004, ZEN content (193.4 μg kg-1) exceeded the allowable level. Variation in the relative air-humidity exerted some effect on the incidence of Fusarium spp. fungi and mycotoxin content in wheat grain. The weather conditions at harvesting contributed to an increase in the contents of Fusarium fungi and DON and ZEN mycotoxins produced by them in winter wheat grain. This risk factor increases the threat to human and animal health.

10

Mycological analysis of cereal samples and screening of Fusarium strains' ability to form deoxynivalenole [DON] and zearalenone [ZEA] mycotoxins - a pilot study

63%

Klyszejko A., Kubus Z., Zakowska Z.

Polish Journal of Microbiology

|

2005

|

tom 54

|

nr Suppl.

Filamentous fungi are cosmopolitan microorganisms found in almost all environments. It should be pointed out that occurance of moulds on food or feed may cause health disorders in humans and animals. Mycoflora appears as a source of toxic methabolites, mycotoxins, which hepatotoxic, genotoxic, nefrotoxic and carcinogenic abilities were already proven in several studies. Hense mycological analysis of cereal grains raises as an important manner in evaluation of food and feed health features. Among the most frequent cereal contaminants Alternaria, Aspergillus, Fusarium and Penicillium strains are mentioned. Due to their ability to grow on cereals during both its field growth and storage, Fusarium moulds occure to be an important contamination factors in food and feed industry. In this study Fusarium strains isolates from wheat and maize were examined in order to recognize their abilities to produce two toxins: zearalenon (ZEA) and deoxynivalenole (DON). Mycological analysis shown differentiation within fungal microflora occuring in samples of different storage conditions, where Fusarium strains represented aproximately 20-70% of all mould species present. In purpose of Fusarium strains species evaluation, isolates were mycologically analysed. In the second step of the project, toxicological screening of isolates was performed using Thin Liquid Chromatography (TLC) evaluating toxigenic potential of single strains' production of ZEA and DON. This data gives the possibility of pointing the most toxigenic strains and also shows differentiations in their occurance in cereals. This paper presents introductory research data, which can be useful in recognition of cereal contamination with moulds and their toxic methabolites.

11

Influence of deoxynivalenol on mitosis of root tip cells of wheat seedlings

63%

Wisniewska H., Chelkowski J.

Acta Physiologiae Plantarum

|

1994

|

tom 16

|

nr 3

12

Relevance of the Fusarium toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

63%

Seeling K., Danicke S.

Journal of Animal and Feed Sciences

|

2005

|

tom 14

|

nr 1

13

Yield reduction and mycotoxin accumulation in barley doubled haploids inoculated with Fusarium culmorum [W.G.Sm.] Sacc.

63%

Adamski T., Chelkowski J., Golinski P., Kaczmarek Z., Kostecki M., Perkowski J., Surma M., Wisniewska H.

Journal of Applied Genetics

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1999

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tom 40

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nr 2

14

Effect of different doses of deoxynivalenol on metabolism in broiler chickens

63%

Faixova Z., Faix S., Borutova R., Leng L.

Bulletin of the Veterinary Institute in Puławy

|

2007

|

tom 51

|

nr 3

The experiment was conducted to investigate the effect of different doses of deoxynivalenol on plasma indices of broiler chickens. Forty-two one-day-old male broiler chicks were fed 1 of 3 diets containing deoxynivalenol (DON) for 42 d. The diets included: (1) control (0.2 ppm of deoxynivalenol), (2) low level of deoxynivalenol (1 ppm of DON), and (3) high level of deoxynivalenol (3 ppm of DON). Then, all the birds were sacrificed and blood samples for biochemical analyses were collected. The mycotoxin doses in diets were verified using gas chromatography-mass spectrometry. The administration of 1 ppm of DON altered total protein, triglycerides, free glycerol, and potassium levels. Dietary addition of 3 ppm of DON resulted in altered calcium, potassium, total protein, triglycerides, along with free glycerol levels, and aspartate aminotransferase activity. No biochemical parameter, however, responded to increased DON concentration in the diet. The feeding of DON-containing diets did not significantly alter plasma chloride, cholesterol, and albumin levels or aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase activities. It was concluded that both levels of deoxynivalenol in the diets tested significantly affected protein and lipid metabolism in broiler chicks.

15

Metoda oznaczania mikotoksyn w ziarnie kukurydzy

51%

Sadowski J., Golebiowska H., Wysocki A.

Progress in Plant Protection

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2010

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tom 50

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nr 4

The aim of investigation was to work out the analytical method of determination of mycotoxins such as: deoxynivalenol, nivalenol and zearlenon in maize grain samples. The analytical procedure consisted of three elementary processes: extraction of analyzed substances from matrix, cleaning of extract using SPE (Solid Phase Extraction) column with C18 active solid and final determination using high performance liquid chromatography with UV detection. The detection limit (LOD) of all tested mycotoxins was 0.002 mg/kg. The range of fortified recoveries of the method was 92–95%.

16

Influence of low doses of deoxynivalenol on histopathology of selected organs of pigs

51%

Zielonka L., Wisniewska M., Gajecka M., Obremski K., Gajecki M.

Polish Journal of Veterinary Sciences

|

2009

|

tom 12

|

nr 1

Deoxynivalenol is one of mycotoxins that are most frequently determined in animal feed manufactured in Poland. The examination of histopathological lesions concomitant with deoxynivalenol intoxication is difficult because of the common, often synergistic, reaction of this mycotoxin with other toxins, such as zearalenone or ochratoxin A, which has a strong nephrotoxic activity. The possibility of estimating histopathological lesions in the course of intoxication with pure toxin at various doses is therefore of interest. Dosages used in this experiment relate to clinical cases observed in feeding the animals with whole ration feed obtained by processing feedingstuffs contaminated with Fusarium moulds. However, concerning the fact of one-shot administration of clinically pure toxin, the main question was if it was a sufficient dose to cause changes in the histopathological picture of gastrointestinal tract organs. The experiment was carried out on 12 nursery pigs of mixed breed (Polish White Large x Polish White Ear-pendent) with an average body weigh of 35 kg. The experimental nursery pigs were divided into 3 groups: group I (n=4) – control; group II (n=4) – DON administered at a dose of 0.2 mg/kg b.w.; group III (n=4) – DON administered at a dose of 0.4 mg/kg b.w. After slaughter of the animals, macroscopic examination was performed and segments of duodenum, jejunum, ileum, liver and mesenteric lymph nodes were sampled and assigned for histopathological examination. The results obtained equate to the clinically observed signs in swine production involving some nutrient metabolism disturbances in the gastrointestinal tract in the course of deoxynivalenol mycotoxicosis. Histopathological examination of segments of the duodenum, the jejunum, the ileum, the liver and the lymph nodes indicate that the regressive lesions are more expressed in the experimental group treated with the highest concentration of deoxynivalenol.

17

Histological estimation of the small intestine wall after administration of feed containing deoxynivalenol, T-2 toxin and zearalenone in the pig

51%

Obremski K., Zielonka L., Gajecka M., Jakimiuk E., Bakula T., Baranowski M., Gajecki M.

Polish Journal of Veterinary Sciences

|

2008

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tom 11

|

nr 4

339-345

Fusarium spp. moulds are common in moderate climate regions of North America, Asia and Europe. They produce hepatotoxic and nephrotoxic mycotoxins, acting like estrogens, impairing heniopoesis and immunosuppressing. Actively dividing skin cells, lymphatic tissue, haemopoetic tissue and gastrointestinal tissue are the most sensitive for these trichothecenes action. The mucosal membrane of the gastrointestinal tract is the first barrier of the organism contacting with foreign antigens like feed proteins, natural toxins, saprophytic and pathogenic microflora and mycotoxins. The aim of this study was to perform histological estimation of the porcine small intestine after short term intoxication with low doses of deoxynivalenol (DON), T-2 toxin (T-2) and zearalenone (ZEA) obtained from wheat naturally contaminated with Fusarium moulds. Experimental pigs (n=5) were fed for 14 days feed containing DON, T-2 and ZEA (28.9, 11.5 and 33.2 μg kg-1 of feed). On the last day of the experiment, the animals were euthanised and samples of the jejunum were collected for histological examination. In the experimental pigs, normally developed intestinal villi and crypts were found. However, number of acidophilic granulocytes in the mucous membrane and decreased numbers of goblet cells, increased numbers of endothelial lymphocytes and numerous plasma cells in intestinal epithelium was observed. On the surface of the intestinal epithelium the glycocalyx was poorly developed. The results obtained suggest that short term intoxication with low doses of DON, T-2 and ZEA does not cause significant changes in the histological structure of the small intestine in the pig. However, low concentrations of DON. T-2 and ZEA probably influence enterocytes metabolism and evoke inflammation of the mucous membrane of the small intestine.

18

Evaluation of selected indicators of immune response (IL-1B, IL-4, IL-6, SAA and HP) in pigs fed diets containing deoxynivalenol, T-2 toxin, and zearalenone

51%

Zielonka L., Zwierzchowski W., Obremski K., Gajecka M., Jakimiuk E., Gajecki M.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

|

nr 4

The objective of this study was to examine the clinical significance of selected indicators of immune response (IL-lß, 1L-4, IL-6. SAA, and Hp) in short-term combined intoxication exposure of pigs to low doses of deoxynivalenol, T-2 toxin, and zearalenone present in naturally contaminated feedstuffs during grain growth. Statistically significant differences were not noted after 14 d of feeding diets contaminated with the above mycotoxins. The mycotoxins did not induce inflammatory processes. The subclinical form of combined mycotoxicosis could be due to incorrect immunological response. The identification of the underlying cause would support the development of new methods for the prevention of combined mycotoxicosis.

19

The effect of selected agroecological factors on health of maize cultivars

51%

Golebiowska H., Weber R., Plaskowska E.

Electronic Journal of Polish Agricultural Universities. Series Agronomy

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2014

|

tom 17

|

nr 4

20

Maize plants infestation by Fusarium spp. and deoxynivalenol in genetically modified corn hybrid and traditional maize cultivars

51%

Selwet M.

Polish Journal of Microbiology

|

2011

|

tom 60

|

nr 4

The objective of the performed investigations was to isolate pathogenic fungi from contaminated maize cobs, to assess the appearance of maize cob fusariosis and to determine grain contamination with deoxynivalenol in the cultivation of genetically modified maize containing a gene resistance against European corn borer (Ostrinia nubilalis Hbn) as well as selected non-modified cultivars. The plant material comprised the following genetically modified maize cultivar: DKC 3421 YG (MON 810) and non-modified cultivars obtained from Smolice Plant Breeding Ltd., IHAR Group: Junak (FAO 210–220), Prosna (FAO 220), SMH (FAO 230), Baca (FAO 220). Prior to harvesting, the occurrence of maize cob fusariosis was determined in the 89 (BBCH) developmental ripening stage. Microbiological assessment was carried out on grains selected from cobs characterized by various pathological symptoms. In 2008, a total of 133 isolates was obtained from the examined samples of infected maize plants, of which 51 isolates were species-identified, while in 2009, the total of 123 isolates were determined, of which 63 were species-identified. In both experimental years, the majority of isolates contained fungi from the Fusarium genus. The performed analysis of mean levels of cob contamination by fusarioses revealed that DKC 3421 YG (MON 810) and SMH (FAO 230) cultivars showed the smallest levels of contamination as well as the lowest percent of cob contamination per plant, while Junak (FAO 210–220) and Baca (FAO 220) cultivars were characterized by the highest degree of contamination. The lowest deoxynivalenol concentrations were determined in years 2008 and 2009 in the case of the DKC 3421 YG (MON 810) cultivar, whereas Prosna (FAO 220) cultivar was characterized by the highest deoxynivalenol concentration.

Ograniczanie wyników

Influence of Fusarium culmorum infection and its metabolite deoxynivalenol on membranes stability in barley seedlings

Effect of deoxynivalenol and degradation enzyme of growth performance and immune responses in mule ducks

Influence of low doses of deoxynivalenol applied per os on chosen indexes of immune response in swine

The rapid acoustic screening of deoxynivalenol [DON] in grain

Influence of deoxynivalenol on elektrolyte leakage in cereal seedling leaves

Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays

The biotransformation of chosen mycotoxins

A survey of deoxynivalenol and zearalenone content in commercial dry foods for growing dogs

Fusarium mycotoxins in Lithuanian cereals from the 2004-2005 harvests

Mycological analysis of cereal samples and screening of Fusarium strains' ability to form deoxynivalenole [DON] and zearalenone [ZEA] mycotoxins - a pilot study

Influence of deoxynivalenol on mitosis of root tip cells of wheat seedlings

Relevance of the Fusarium toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

Yield reduction and mycotoxin accumulation in barley doubled haploids inoculated with Fusarium culmorum [W.G.Sm.] Sacc.

Effect of different doses of deoxynivalenol on metabolism in broiler chickens

Metoda oznaczania mikotoksyn w ziarnie kukurydzy

Influence of low doses of deoxynivalenol on histopathology of selected organs of pigs

Histological estimation of the small intestine wall after administration of feed containing deoxynivalenol, T-2 toxin and zearalenone in the pig

Evaluation of selected indicators of immune response (IL-1B, IL-4, IL-6, SAA and HP) in pigs fed diets containing deoxynivalenol, T-2 toxin, and zearalenone

The effect of selected agroecological factors on health of maize cultivars

Maize plants infestation by Fusarium spp. and deoxynivalenol in genetically modified corn hybrid and traditional maize cultivars