Wyniki wyszukiwania - AGRO

1

Evaluation of the effects of antibiotics on cytotoxicity of EGFP and DsRed2 fluorescent proteins used for stable cell labeling

100%

Zakrzewska K. E., Samluk A., Pluta K. D., Pijanowska D. G.

Acta Biochimica Polonica

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2014

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tom 61

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nr 4

2

The mechanism of interleukin-1 cytotoxicity in HeLa cells

100%

Doszczak M., Pierzchalski A., Holtmann H., Bigda J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

3

DNA-binding properties and cytotoxicity of biscationic extended diphenylfurans derivatives: effects of the terminal basic side chains

88%

Bielawski K., Bielawska A., Wolczynski S.

Folia Histochemica et Cytobiologica. Supplement

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2001

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tom 39

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nr 1

4

Influence of culture medium pH on cytotoxicity of CD95L in vitro

88%

Grzela T., Lazarczyk M., Oldak M., Korczak-Kowalska G.

Folia Histochemica et Cytobiologica

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2001

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tom 39

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nr 1

5

Heparin enhances generation of natural killer activity in vitro

88%

Wasik M., Gorski A.

Archivum Immunologiae et Therapiae Experimentalis

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1994

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tom 42

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nr 1

6

Cytotoxicity of cyclopentenyl cytosine towards neuroblastoma cell line cells

88%

Bierau J., Van Gennip A. H., Van Kuilenburg A. B. P.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

7

Influence of G2 arrest on the cytotoxicity of DNA topoisomerase inhibitors toward human carcinoma cells with different p53 status

88%

Bozko P., Larsen A. K., Raymond E., Skladanowski A.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-incubation in drug-free medium. RKO cells expressing wild-type, functional p53 arrested the cell cycle progression in both the Gj and G2 phases of the cell cycle whereas the RKO/E6 cells, which lack functional p53, only arrested in the G2 phase. Growth-arrested cells did not resume proliferation even after prolonged incubation in drug-free medium (up to 96 h). To evaluate the importance of the cell cycle arrest on cellular survival, a non-toxic dose of UCN-01 (100 nM) was added to the growth-arrested cells. The addition of UCN-01 was accompanied by mitotic entry as revealed by the appearance of condensed chromatin and the MPM-2 phosphoepitope, which is characteristic for mi- totic cells. G2 exit and mitotic transit was accompanied by a rapid activation of caspase-3 and apoptotic cell death. The influence of UCN-01 on the long-term cytotoxic effects of the two drugs was also determined. Unexpectedly, abrogation of the G2 arrest had no influence on the overall cytotoxicity of either drug. In contrast, addition of UCN-01 to cisplatin-treated RKO and RKO/E6 cells greatly increased the cytotoxic effects of the alkylating agent. These results strongly suggest that even prolonged cell cycle arrest in the G2 phase of the cell cycle is not necessarily coupled to efficient DNA repair and enhanced cellular survival as generally believed.

8

Redox properties and prooxidant cytotoxicity of a neuroleptic agent 6,7-dinitrodihydroquinoxaline-2,3-dione (DNQX)

75%

Sarlauskas J., Nemeikaite-Ceniene A., Miseviciene L., Krikstopaitis K., Anusevicius Z., Cenas N.

Acta Biochimica Polonica

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2013

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tom 60

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nr 2

In order to characterize the possible mechanism(s) of cytotoxicity of a neuroleptic agent 6,7-dinitrodihydroquinoxaline-2,3-dione (DNQX) we examined the redox properties of DNQX, and its mononitro- (NQX) and denitro- (QX) derivatives. The irreversible electrochemical reduction of the nitro groups of DNQX was characterized by the reduction peak potentials (Ep,7) of -0.43 V and -0.72 V vs. Ag/AgCl at pH 7.0, whereas NQX was reduced at Ep,7 = -0.67 V. The reactivities of DNQX and NQX towards the single-electron transferring enzymes NADPH:cytochrome P-450 reductase and NADPH:adrenodoxin reductase/adrenodoxin complex were similar to those of model nitrobenzenes with the single-electron reduction potential (E17) values of -0.29 V - -0.42 V. DNQX and NQX also acted as substrates for two-electron transferring mammalian NAD(P)H:quinone oxidoreductase (DT-diaphorase). The cytotoxicity of DNQX in bovine leukemia virus-transformed lamb kidney fibroblasts (line FLK) was prevented by antioxidants and an inhibitor of NQO1, dicoumarol, and was enhanced by the prooxidant alkylating agent 1,3-bis(2-chloromethyl)-1-nitrosourea. A comparison with model nitrobenzene compounds shows that the cytotoxicity of DNQX and NQX reasonably agrees with the ease of their electrochemical reduction, and/or their reactivities towards the used enzymatic single-electron reducing systems. Thus, our data imply that the cytotoxicity of DNQX in FLK cells is exerted mainly through oxidative stress.

9

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Cytotoxic flavonoids from Diplotaxis harra (Forsk.) Boiss. growing in Sinai

75%

Mohammed M. M. D., El-Sharkawy E. R., Matloub A. A.

Herba Polonica

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2011

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tom 57

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nr 3

As a part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forsk.) Boiss. was in vitro investigated for its cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50=4.65, 12.60 and 17.90 μg/ml, respectively. Doxorubicin (+ve control) showed in vitro cytotoxic activity with IC50=3.64, 4.57 and 2.97 μg/ml, respectively. The phenolic-rich fraction of the EtOH extract was subjected to further fractionation, which led to the isolation of five flavonoids identified as quercetin, quercetin 3-O-β-glucoside, isorhamnetin 7-O-β-glucoside, apigenin 3-O-β-rhamnoside and kaempferol 3-O-β-glucoside, according to its’ spectral data and comparison with the literature. Furthermore, the isolated flavonoids showed in vitro cytotoxicity against HCT116 cell line with IC50=20.1, 24.3, 22.8, 23.4 and 41.9 μg/ml as determined by MTT method.

10

The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

75%

Galanty A., Michalik M., Sedek L., Podolak I.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 4

585-598

We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.

11

Cytotoxicity of anticancer aziridinyl-substituted benzoquinones in primary mice splenocytes

75%

Miliukiene V., Nivinskas H., Cenas N.

Acta Biochimica Polonica

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2014

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tom 61

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nr 4

12

Oxidative stress and cytotoxicity elicited lipid peroxidation in hemocytes of Bombyx mori larva infested with dipteran parasitoid, Exorista bombycis

75%

Pooja M., Pradeep A. N. R., Hungund S. P., Sagar C., Ponnuvel K. M., Awasthi A. K., Trivedy K.

Acta Parasitologica

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2017

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tom 62

|

nr 4

13

Antiviral activity of novel oseltamivir derivatives against some influenza virus strains

75%

Kocik J., Kolodziej M., Joniec J., Kwiatek M., Bartoszcze M.

Acta Biochimica Polonica

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2014

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tom 61

|

nr 3

14

Phytochemical analysis and cytotoxicity evaluation of flowering buds of Bauhinia variegata L.

75%

Abbasi A. S., Najam-Us-Saqib Q., Atta-Ur-Rehman, Nisar-Ur-Rahman

Herba Polonica

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2021

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tom 67

|

nr 3

15

Cytotoxic activity of Serratia marcescens clinical isolates

75%

Krzyminska S., Raczkowska M., Kaznowski A.

Polish Journal of Microbiology

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2010

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tom 59

|

nr 3

Twenty Serratia marcescens isolates from clinical specimens were examined for their cytotoxic activity on four cell lines (HEp-2, Vero, CHO, J774). Most of the isolates were found to be cytotoxic to CHO (70%), Vero (75%) and HEp-2 cells (90%). CHO cells were the most sensitive to cell-free supematants, followed by HEp-2 and Vero cells. Two strains produced cytotonic toxins which caused elongation of CHO cells. Moreover, twelve isolates (60%) revealed cytotoxic potential to macrophage cell line J774. The results indicate that these bacteria may destroy phagocytes and epithelial cells, which may lead to spread within the host.

16

Determination of melamine cytotoxicity

75%

Radko L., Minta M., Stypula-Trebas S., Zmudzki J.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

|

nr 2

223-228

Cytotoxic potential of melamine was evaluated with the use of two in vitro models i.e. cell cultures of rat hepatoma (line FaO) and rat skeletal muscle (line L6). The cultures were exposed for 24, 48, and 72 h to melamine at eight concentrations, ranged from 0.01 to 10 mM. Four different assays were applied in which various biochemical endpoints were assessed: mitochondrial activity - MTT reduction assay, proliferation - Commassie Brilliant Blue (CBB) dye binding assay, lysosomal activity - neutral red uptake (NRU) assay, and membrane integrity - LDH release assay. Effective concentrations (EC₂₀, EC₅₀, EC₈₀) were calculated from concentration-response curves, and then they were averaged over three independently conducted experiments. It was found that MTT assay was the most sensitive to this compound. After 48 h exposure EC₅₀ values (mM, mean ± SD) for FaO and L6 cells were 6.4 ± 0.62, and 8.2 ± 1.51, respectively. The inhibition of lysosomal activity measured by NRU assay, and damage of plasma membrane measured by LDH assay were detected in L6 (but not in FaO) cells; however, the effects took place after longer (72 h) exposure. At that time EC₅₀ values were 5.2 mM and 9.2 mM for NRU and LDH, respectively. In spite of the low cytotoxicity of melamine, more studies are needed for hazard identification and characterization of the compound.

17

Influence of cultivation conditions of white cabbage on the content of bioactive compounds

75%

Kusznierewicz B., Piekarska A., Poleska-Muchlado Z., Milewczyk A., Kujawska N., Namiesnik J., Bartoszek A.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

|

nr Suppl.1

18

The effect of magnetic field on in vitro development of fungus-like organisms Saprolegnia parasitica on selected microbiological media

75%

Mazurkiewicz-Zapalowicz K., Twaruzek M., Formicki K., Korzelecka-Orkisz B., Wolska M., Tanski A., Szulc J.

Electronic Journal of Polish Agricultural Universities. Series Fisheries

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2015

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tom 18

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nr 2

19

Cytotoxicity and inhibitory properties against topoisomerase II of doxorubicin and its formamidine derivatives

75%

Kik K., Studzian K., Wasowska-Lukawska M., Oszczapowicz I., Szmigiero L.

Acta Biochimica Polonica

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2009

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tom 56

|

nr 1

135-142

This work was undertaken to compare cytotoxicity, DNA damaging properties and effect on DNA cleavage by topoisomerase II of the anthracycline drug doxorubicin (DOX) and its two derivatives with a formamidino group containing a cyclic amine moiety such as morpholine (DOXM) or hexamethyleneimine (DOXH). The tetrazolium dye colorimetric assay was used to determine the cytotoxic activity of anthracyclines toward L1210 leukemia cells. DNA damage was measured by alkaline elution technique. The effect of anthracyclines on DNA cleavage was studied in a cell-free system containing supercoiled pBR322 DNA and purified human topoisomerase II. The cytotoxicity data and the results of studies on the mechanism of DNA break formation by anthracyclines at the cellular level and in the cell-free system showed that the presence of the formamidino group in the doxorubicin molecule reduced its ability to stimulate DNA cleavage by DNA topoisomerase II. Conclusion: DNA topoisomerase II is not a primary cellular target for DOXM or DOXH. An advantageous feature of formamidinoanthracyclines is their mechanism of cytotoxic action which is not related to the inhibition of DNA topoisomerase II. Therefore this class of anthracyclines seems to be a good source for selection of an anticancer drug directed toward cancer cells with the developed multidrug resistance attributed to the presence of altered DNA topoisomerase II.

20

Oxidative stress-mediated cytotoxicity of cadmium in chicken splenic lymphocytes

75%

Li J.-L., Li H.-X., Li S., Tang Z.-X., Xu S.-W., Wang X.-L.

Polish Journal of Environmental Studies

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2010

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tom 19

|

nr 5

Cadmium (Cd), a potent immunotoxicant, has been reported to affect immunocytes both in humans and in rodents. However, whether such an effect exists in birds is still unclear. To delineate the cytotoxicity of Cd on the bird immunocytes in vitro, chicken splenic lymphocytes were exposed to Cd. A significant increase in the occurrence of cell damage was observed in chicken splenic lymphocytes following Cd administration. The enhanced level of LPO and the overproduction of ROS suggested that Cd caused oxidative stress in chicken splenic lymphocytes. A pronounced inhibition of SOD and GPx was seen after being indicated with 10 μM Cd for 36h or indicated with 15 μM Cd for 24 h. In addition, DNA damage and apoptosis was observed in chicken splenic lymphocytes treated with Cd. The degree of DNA damage and the number of apoptotic cells rose in a time- and dose-dependent manner. These results clearly demonstrate that chicken splenic lymphocytes appear to be more susceptible than mammalian immunocytes to the adverse effects of Cd. The oxidative stress and subsequent DNA damage and apoptosis induced by Cd are important mechanisms of Cd cytotoxicity to bird immunocytes.

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