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The lupine (Lupinus luteus cv. Ventus) cDNA clones encoding homologues of cyclin (CycBlfi, CycBl;3, CycBl;4) have been Isolated from cDNA library pre­pared from roots inoculated with Bradyrhizobium lupint. Comparison of the deduced amino-acid sequences of CycBlg, CycBl;3, CycBl;4 and previously described CycBl;l (Deckert et al 1996, Biochimie 78, 90-94) showed that they share 46-65% of identical amino acids. The presence of conserved residues (Renaudin et al., in The Plant Cell Cycle, in the press; Renaudin et al.. Plant Mol. Biol., in the press) along with phylogenetic analysis of known plant cyclins revealed that the four lupine sequences belong to subgroup I of B-like mitotic cyclins.
This study examined the effects of different exogenous auxins and cytokinins at 0.1-5.0 mg·l-1 concentration on shoot cuttings of two H. perforatum clones transformed with a wild agropine strain of A. rhizogenes and one untransformed clone. Their sensitivity to the auxins varied and showed concentration-dependent behavior, and the response to auxins differed between the transgenic clones. The number of cuttings of transgenic clones capable of root formation, and the onset of rooting on most of the media with auxins lagged behind the control. The number of differentiated shoots of the transgenic clones on hormone-free medium was two to three times higher than that of the untransformed control. Regenerated shoots of the transgenic clones on basal medium branched much less than the nontransgenic clone. The transgenic and control clones differed in their ability to form shoots on media supplemented with cytokinins. Increased cytokinins led to differentiation of shorter shoots with fewer leaf pairs. Because gene expression studies have shown integration of rolABC genes, their possible impact on the type of morphogenetic response is discussed.
The flowering and graft mortality of Norway spruce clones of Istebna origin was only slightly different from clones of other origin in the same seed orchard. The negative effect ofmoving Istebna clones from the south to the north of their origin was probably compensated by moving them from a higher altitude to lowland. There is a danger that the complete loss of several clones, observed in the seed orchard with time,may really decrease the expected level of genetic variation in the progeny. It is recommended that the mortality of grafts and the reduction in the number of clones in the seed orchard should be under permanent observation.
Rootability of Dalbergia sissoo Roxb. cuttings of mature tree originated clonal hedge bed were tested and compared to the same from seedling originated hedge bed. Mature tree originated cuttings were found potential for rooting in low-cost non-mist propagator without any rooting hormone. Steckling growth in nursery and in the field was found promising which is a complete indication for its successful application by tree planters. It will be a new research avenue to get disease free germplasm for the frustrated plantation managers and scientists due to die back of this tree through out the South Asian region.
The aim of the study was to compare the effect of poplar clones Hybrid 275 and Robusta on soil cover, based on their litter fall. In 2000-2002 studies on plant litter fall and leaf decomposition were conducted at two 17-year-old poplar plantations in the Wierzbiczany forest district (Northern Poland). Both clones produced similar amounts of litter fall per tree of about 11 kg/year. Leaves were the main component of the litter fall (about 80% of total mass). Plant litter fall at the poplar plantations was rich in nutrients, lacking only phosphorus. Litter fall of the analysed poplar clones decomposed rapidly. Leaves of Hybrid 275 decomposed slightly faster than those of Robusta. Leaves of Robusta decomposed more slowly because of their lower content of nitrogen, higher content of lignin and their morphology – harder and waxier. Over the 20 month period more than 70% of the mass of Hybrid 275 leaves and 50% of the Robusta leaves decomposed. The absolute accumulation of nitrogen was observed in the decomposing leaves, along with a narrowing of the C/N ratio and a more rapid decrease in potassium content than the loss of mass. Based on the properties of litter fall, we can conclude that both poplar clones would have a favourable effect on soil cover.
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