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The aim of this paper was to analyse the biochemical phenotypes of Candida albicans colonising the upper respiratory tract in 100 patients with chronic hepatitis C from group I (without antiviral therapy) and from group II (treated with peginterferon and ribavirin). The ability of the assimilation of carbon from various substrates (assimilation phenotypes) or activity of hydrolytic enzymes (biotypes) of 61 C. albicans isolates were estimated using API 20 C AUX and API ZYM microtests, respectively. Among 30 isolates of C. albicans from the group I, seven assimilation phenotypes and six biotypes were determined, while among 31 isolates from the group II - eleven assimilation phenotypes and five biotypes. The most frequently isolated assimilation phenotype in both groups of patients with API numerical profile of 2576174 comprised about 50%-70% of all phenotypes. The predominant biotype E belonging to the classification of Williamson comprised about 39%-50% of all biotypes. Our results and those from the literature suggest that C. albicans biotypes but not assimilation phenotypes may be related with some diseases. However, this requires further detailed studies.
Nutritional status of patients infected with hepatitis was studied. Twenty four male patients suffering from chronic hepatitis C virus infection were selected from those attending the outpatients clinic in El-Harm Hospital. Also 21 male healthy volunteers were taken as controls. Clinical and dietary status of all individuals was examined. There were 4 cases with cirrhosis associated with ascites, 8 cases with splenomegaly, 2 cases had liver cirrhosis and one case had hematuria. Significantly higher values for the activity of AST, ALT and bilirubin were reported for patients suffering from chronic hepatitis. Patients consumed significantly less calories, proteins and fats compared to controls. Patients also consumed less fiber but the differences were not significant. The intake of phosphorus, zinc and magnesium of hepatitis patients was significantly lower than that of controls. No significant change in the intake of iron, potassium or calcium was reported. Significant drop in the intake of vitamins B1, B2, niacin, B6, B12, folate and vitamin E was reported for patients suffering from hepatitis. Intake of essential amino acids of hepatitis patients was significantly lower as compared with controls. The present study indicates that those patients need special dietary management. Improvement of patients appetites to increase their food intake from carbohydrates and protein but not fat must be considered. Also, the diets must be supplemented with calcium, phosphorus and B-vitamins but not iron. Reducing the intake of iron is essential to prevent iron overdose with its clinical complications. Those patients need special programme of nutrition education to help them to select the most beneficial food they need to escape the complication of the disease.
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