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Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

100%

Amini J., Agapoor Z., Ashengroph M.

Journal of Plant Protection Research

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2016

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tom 56

|

nr 3

In this study, about 112 isolates of Streptomyces were isolated from chickpea rhizospheric soils. Among the isolated strains, five showed strong inhibitory effects against chickpea Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris in vitro using plate assay and selected for further studies. The selected strains were identified as Streptomyces spp. based on morphological and biochemical characterization as well as 16S rDNA sequences analysis. Our results assigned them to strains related to genus of Streptomyces. In vitro, antagonistic effects of Streptomyces strains against the disease were evaluated through the dual-culture method, volatile and non-volatile metabolites, siderophore, protease and chitinase production. All bacterial strains inhibited mycelial growth of the pathogen ranging from 26 to 44.2% in dual culture assay. The non-volatile extract of five of the Streptomyces strains inhibited more than 50% growth of the pathogen, whereas volatile compounds were less effective on mycelial growth inhibition (20.2 to 33.4%). The ability of the biocontrol agents to produce siderophore and protease were varied, whereas, production of chitinase was detected for all strains. Results of the greenhouse assay indicated that all biocontrol agents reduced disease severity (ranging from 38.7 to 54.8%). Accordingly, strain KS62 showed higher control efficacy (54.8%). In addition, the biomass of chickpea plants (plant height and dry weight) significantly increased in plants treated with Streptomyces strains compared to non-bacterized control. The results of this study showed that it may be possible to manage chickpea Fusarium wilt disease effectively by using Streptomyces species, as biocontrol agents. Therefore, evaluating their efficiency under field conditions is needed.

2

Induced mutagenesis in Cicer arietinum

100%

Mullainathan L., Umavathi S.

International Letters of Natural Sciences

|

2014

|

tom 07

The traditional varieties of chick pea have low potentiality and restricted variability with respect to economic characters. Broadening the genetic base for crop improvement can be quickly achieved through induced mutagenesis. The present study was undertaken in order to comparing the effectiveness and efficiency of mutagens on Cicer arietinum. In this regard, Co – 4 variety of chick pea was subjected to different dose/concentration of Gamma rays (20, 30, 40, 50 and 60 kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM) for inducing mutation. Mutagenic effectiveness and efficiency was calculated based on biological damage in M1 and chlorophyll mutations in M2. The results indicated that, mutagenic effectiveness increased with the increase in dose/concentration of mutagen. Intermediate treatments in general were found more efficient in causing less biological damage and inducing maximum amount of mutations. It shows that the chemical mutagens are more effective and efficient than physical mutagen for inducing mutation in Chick pea.

3

Expansion in chickpea (Cicer arietinum L.) seed during soaking and cooking

100%

Sayar S., Turhan M., Koksel H.

International Agrophysics

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2016

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tom 30

|

nr 1

4

Appearance of different phosphatase forms and phosphorus partitioning in nodules of chickpea [Cicer arietinum L.] during development

100%

Gupta A. K., Kaur V., Kaur N.

Acta Physiologiae Plantarum

|

1998

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tom 20

|

nr 4

Acid and alkaline phosphatase and phytase activities were determined in the bacteroid free fractions of chickpea (Cicer arietinum L.) nodules at 15 days intervals, from 40 days after sowing (DAS) to 85 DAS. In general, the activities and specific activity of both the acid and alkaline phosphatases declined at 55 DAS. Out of the various substrates studied, ATP was the best substrate for both phosphatases. Activities of phosphatases with glucose-6-phosphate and fructose-6-phosphate were low in comparison to these with fructose 1,6 bisphosphate. The efficiency of acid phosphatase for utilizing fructose 1,6 bis phosphate as a substrate increased with nodule development. A fructose 1,6 bis phosphate specific acid phosphatase with elution volume to void volume (Ve/Vo) ratio of around 2.0 was observed in mature nodules (80 DAS). Acid phosphatase at 40 DAS was resolved into two peaks which were eluted at Ve/Vo of about 1.5 and 1.8. However, at 60 DAS the peak with Ve/Vo of 1.5 could not be detected. With ATP as substrate, a high (Ve/Vo of 1.2) and low MM form (Ve/Vo of 2.1) alkaline phosphatases were observed at 40 DAS however at 60 DAS stage only one peak with Ve/Vo of 1.7 was detected. Although, a low activity of acid phytase was observed in nodules at all stages of development but neither alkaline phytase nor phytic acid could be detected. It appears that the nodules acquire inorganic phosphate from the roots. The higher content of water soluble organic phosphorus in mature nodules could be due to the low activities of phosphatases at maturity.

5

Fungi isolated from chickpea [Cicer arietinum L.] seeds

100%

Mazur S., Nawrocki J., Kucmierz J.

Vegetable Crops Research Bulletin

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2001

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tom 54

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nr 2

6

Effect of the biological and simulated moisture contents of chickpea seeds [Cicer arietinum L.] on their physical properties

100%

Rataj V., Jech J., Fuska M., Hlavacova Z., Sosnowski S.

International Agrophysics

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1999

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tom 13

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nr 3

7

The role of 24-epibrassinolide induced antioxidant defence during germination under salinity in chickpea

84%

Yalcinkaya T., Turkan I., Sekmen A., Ozgur R.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

8

Serological and molecular detection of bean leaf roll and chickpea chlorotic stunt luteoviruses in chickpea from Iran

84%

Hajiyusef T., Shahraeen N., Maleki M.

Journal of Plant Protection Research

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2017

|

tom 57

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nr 2

Chickpea (Cicer arietinum L.) is an important legume crop and widely cultivated in northwestern provinces of Iran. During a survey in the 2015 growing season a total of 170 selected chickpea plants with general yellowing symptoms including stunting and leaf bronzing were collected. Serological Elisa and tissue blot immunoassay (TIBA) tests revealed the presence of Bean leaf roll virus (BLRV) and Chickpea chlorotic stunt virus (CpCSV) as the predominant viruses in the region. Some serologically positive samples of BLRV and CpCSV were selected and rechecked by RT-PCR. Th e results of amplifi ed PCR products using a specifi c pair of primers towards the Cp gene region of the viruses were approximately 413 bp for CpCSV and 391 bp for BLRV. Results obtained from sequence comparison of BLRV (IR-F-Lor-5) isolate form two subgroups with eight other BLRV isolates from GeneBank indicating a high homology of 96% with isolates from Argentina, Germany, Tunisia, USA, Spain, and Colombia. An isolate from Norabad (Iran) (IR-Nor) had 98% homology with HQ840727 Libyan isolate. CpCSV sequence comparison with six other GeneBank isolates indicated 98% homology with isolates from Tunisia and Azerbaijan. Th e overall results of this research revealed the CpCSV and BLRV (luteoviruses) associated with the yellowing disease syndrome of chickpea crops in the surveyed region.

9

Growth promotion and charcoal rot management in chickpea by Trichoderma harzianum

84%

Jyotsna, Srivastava A., Singh R. P., Srivastava A. K., Saxena A. K., Arora D. K.

Journal of Plant Protection Research

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2008

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tom 48

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nr 1

81-92

The strains of Trichoderma harzianum were assessed for their effect on chickpea growth and control of charcoal rot caused by Macrophomina phaseolina in greenhouse assay. T. harzianum strain 25-92 significantly increased the fresh and dry weights by 50-63% and 24-42%, respectively, whereas strain 29-92 increased the fresh weight of chickpea cv. Radhey and Vishwas by 12-30% but not the dry weight in the absence of M. phaseolina. A marked increase in root length was caused by both the strains. In M. phaseolina infested pots, number of lateral roots and branching decreased with nonsignificant change in weight. Significant (P = 0.05) reduction in charcoal rot disease was observed in the pots amended with T. harzianum at all the concentrations. Moreover, 60-40% reduction in disease was recorded after 14 and 28 days in chickpea varieties Radhey and Vishwas. The resistant variety Vijay does not show significant disease. The reduction in disease was more pronounced at higher inoculum concentrations of T. harzianum (107-108 cfu/g). Overall, Trichoderma strain 25-92 improved plant growth and reduced damage in presence of the pathogen. Besides disease control the growth promoting properties of the strain improve the efficacy for commercial application.

10

The effect of locality and sowing term on chosen morphological features of two chickpea (Cicer arietinum L.) cultivars

84%

Poniedzialek M., Jedrszczyk E., Sekara A., Skowera B., Dziamba S.

Folia Horticulturae

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2005

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tom 17

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nr 1

37-46

11

Nutritional variation and drought tolerance in chickpeas (Cicer arietinum L.)

84%

Kahraman A., Ceyhan E., Harmankaya M.

Journal of Elementology

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2015

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tom 20

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nr 2

Pulses are an important dietary constituent in human and animal diets. As well as being a source of income and livestock feed, pulses satisfy 33% of the dietary protein nitrogen (N) needs of humans. Pulses are often exposed to environmental stresses (biotic and abiotic) that decrease their productivity throughout the world. Abiotic stresses (drought, salt, temperature, UV, nutrient deficiency) alone are responsible for more than 50% yield reductions of some major crops. The following examination of drought stress types (no irrigation, early period stress, late period stress, control) of 10 chickpea genotypes from Turkey was carried out for two years (2010 and 2011) in a field experiment set up accoding to a randomized complete block design with three replications and conducted under the ecological conditions prevalent in Konya, Turkey. The investigated nutrition-related parameters were the seed concentrations of protein, fat, ash, fiber, moisture, non-nitrogenous pith substances and minerals. Means achieved under the particular stress types showed that the protein percentage ranged from 19.71% to 19.80%, fat – 4.640 to 4.690%, ash – 2.810 to 2.860%, fiber – 7.360 to 7.400%, moisture – 9.150 to 9.180%, non-nitrogenous pith substances – 56.16 to 56.25%, while the mineral content (mg kg-1) corresponded to the following ranges: 9.700 to 9.980 B, 68.32 to 79.44 for Fe, 8866.3 to 8912.4 for K, 1383.1 to 1410.3 for Mg, 21.99 to 23.85 Mn, 3148.0 to 3192.6 P, 1815.6 to 1835.4 for S and from 25.81 to 28.43 for Zn. In general, the content of protein, magnesium and sulfur showed the lowest values under no irrigation conditions, while the content of ash, non-nitrogenous pith substances, boron, potassium, phosphorus and zinc showed the highest values under no irrigation conditions. Additionally, the nutritional value of chickpea seeds showed significant differences for all of the investigated characteristics viewed in terms of triple interactions (year x stress factor x genotype). The present research results can be useful for farmers, plant breeders, food companies etc., interested in chickpea. Finally, responses of the genotypes to different levels of drought stress were modified by the investigated quality characteristics.

12

Mutagenic effect of gamma rays and EMS on seed germination, seedling height reduction and survivability of chick pea (Cicer arietinum L.) var. Co - 4

84%

Umavathi S., Mullainathan L.

International Letters of Natural Sciences

|

2014

|

tom 11

|

nr 1

The effect of gamma irradiation and EMS treatment on seed germination and seedling height of Chick pea (Cicer arietinum. L.). In this regard Co-4 variety of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50, and 60 kR) and EMS (10, 20, 30, 40 and 50 mM) for inducing mutation. The effect of mutagen was observed on the basis of percentage of seed germination, seedling height reduction at 15th day and survivability. From the result it was observed that, the percentage of seed germination, seedling height reduction at 15th day and survivability were significantly decreased with increasing doses/concentrations of mutagen. The effective doses/ concentrations which caused 50 % growth reduction were 40kR in gamma rays and 30 mM in EMS.

13

Changes in bioactive carbohydrates during dehydration processing on chickpeas

84%

Aguilera Y., Benitez V., Molla E., Lopez-Andreu F. J., Esteban R. M., Martin-Cabrejas M. A.

Polish Journal of Food and Nutrition Sciences

|

2011

|

tom 61

|

nr Suppl.1

14

Assessment of molecular diversity in chickpea (Cicer arietinum L.) rhizobia and structural analysis of 16S rDNA sequences from Mesorhizobium ciceri

84%

Yadav A., Singh A. L., Rai G. K., Singh M.

Polish Journal of Microbiology

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2013

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tom 62

|

nr 3

Molecular diversity studies of 19 rhizobia isolates from chickpea were conducted using simple sequence repeats (SSR) and 16S rDNA-RFLP markers. Phenotypic characterization with special reference to salinity and pH tolerance was performed. These isolates were identified as different strains of Mesorhizobium, Rhizobium, Bradyrhizobium, and Agrobacterium. Twenty SSR loci of Mesorhizobium ciceri, distributed across the other rhizobial genome, clearly differentiated 19 rhizobial isolates. Analogous clustering supported the results of 16S rDNA sequence-based phylogeny. Analysis of the 16S rDNA sequences from M. ciceri strains revealed that nucleotide variables (signature sites) were located at 20 different positions; most of them were present in the first 820 bp region from 5’ terminal. Interestingly, 14 signature sites were located in two main regions, the variable region V1 (nt 527–584), and variable region V2 (nt 754–813). The secondary structure and minimal free energy were determined in these two regions. These results will be useful in characterizing the micro-evolutionary mechanisms of species formation and increase understanding of the symbiotic relationship.

15

Drought stress effects on photosynthesis, chlorophyll fluorescence and water relations in tolerant and susceptible chickpea (Cicer arietinum L.) genotypes

84%

Rahbarian R., Khavari-Nejad R., Ganjeali A., Bagheri A., Najafi F.

Acta Biologica Cracoviensia. Series Botanica

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2011

|

tom 53

|

nr 1

In order to evaluate morphological and physiological traits related to drought tolerance and to determine the best criteria for screening and identification of drought-tolerant genotypes, we grew two tolerant genotypes (MCC392, MCC877) and two sensitive genotypes (MCC68, MCC448) of chickpea under drought stress (25% field capacity) and control (100% field capacity) conditions and assessed the effect of drought stress on growth, water relations, photosynthesis, chlorophyll fluorescence and chlorophyll content in the seedling, early flowering and podding stages. Drought stress significantly decreased shoot dry weight, CO2 assimilation rate (A), transpiration rate (E), and PSII photochemical efficiency (Fv/Fm) in all genotypes. In the seedling and podding stages, PSII photochemical efficiency was higher in tolerant genotypes than in sensitive genotypes under drought stress. Water use efficiency (WUE) and CO2 assimilation rate were also higher in tolerant than in sensitive genotypes in all investigated stages under drought stress. Our results indicated that water use efficiency, A and Fv/Fm can be useful markers in studies of tolerance to drought stress and in screening adapted cultivars of chickpea under drought stress.

16

Physical and chemical induced mutagenesis study for identifying lethality dose in chick pea (Cicer arietinum L.) Var. Co - 4

84%

Umavathi S., Mullainathan L.

International Letters of Natural Sciences

|

2015

|

tom 08

The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD50). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M1 generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD50 values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.

17

Induce physical and chemical mutagenesis for improvement of yield attributing traits and their correlation analysis in Chickpea

84%

Raina A., Laskar R. A., Khursheed S., Khan S., Parveen K., Amin R., Khan S.

International Letters of Natural Sciences

|

2017

|

tom 61

In the current scenario of sky high population and widespread food insecurity, there is an urgent need for productivity improvement of major crops like pulses, one of the most climate friendly and accessible foods for the large poor population of the world. Chickpea is an important pulse crop, having high percentage of nutritional content and is widely used as food across the world. Induced mutagenesis has proved over time as a coherent tool for modern plant breeders to improve the productivity for combating the food insecurity and malnutrition across the globe. Dry and healthy seeds of Cicer arietinum L. var. C-235 were treated with individual and combination doses of gamma rays, sodium azide and hydrazine hydrate to raise the M₁ generation. M₂ seeds harvested from M₁ plants were sown in the nest season. Bio-physiological study in M1 generation showed growth inhibition in the order viz. combination treatment>hydrazine hydrate>gamma rays>sodium azide. In M₂ generation, comparative observations were recorded for morphological variation and quantitative traits to assess the genetic response of the chickpea variety C-235 toward the different concentrations of mutagens. Observations on isolated mutants in M₂ generation revealed that 0.3% HZ + 0.02% SA was most mutagenic while 200 Gy, 0.03% SA and 0.2% HZ were highest in their individual treatment groups. In context to the frequency of morphological mutants studied in M₂ generation, dwarf mutants were found to be highest followed by tall and branching pattern mutants. Highly significant correlation coefficient between yield and other traits showed that the selection for high yielding mutants can be done on the basis of these traits undoubtedly.

18

Microsatellite markers discriminating accessions within collections of plant genetic resources

84%

Kraic J., Gregova E., Jomova K., Hudcovicova M.

Cellular and Molecular Biology Letters

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2002

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tom 07

|

nr 2B

The reliability of microsatellite analyses for discriminating between plant accessions maintained in collections of genetic resources was tested for 53 accessions of barley, 65 of soybean, 49 of chickpea, and 19 of alfalfa. The specific primer pairs used in this study were based on microsatellite DNA sequences surrounded by perfect dinucleotide and imperfect trinucleotide tandem repeat units. The evaluated polymorphic information content, diversity index, and probabilities of identity indicate that there is value in the application of SSR analyses in barley, soybean, and chickpea genetic resource management. Variation between alfalfa genotypes was not revealed at the five analyzed microsatellite loci.

19

Involvement of the photoreceptor phytochrome in light regulation of nitrate reductase in cotyledons of chick-pea [Cicer arietinum L.]

84%

Bueno M. S., Guerra H., Pintos B., Martin L., Villalobos N.

Acta Physiologiae Plantarum

|

1996

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tom 18

|

nr 4

20

Somatic embryogenesis from chickpea [Cicer arietinum L.] inmature cotyledons: the effect of zeatin, gibberellic acid and indole-3-butyric acid

84%

Hita O., Lafarga C., Guerra H.

Acta Physiologiae Plantarum

|

1997

|

tom 19

|

nr 3

Studies were conduced to test the effects of various cytokinins on somatic embryogenesis from chickpea (Cicer arietinum L.) immature cotyledons. Zeatin (13.7 µmol) added, to B5 basal medium, supplemented with 1.5 % sucrose and 0.2 µmol indole-3-acetic acid, was the most effective cytokinin. Lobular structures obtained from cotyledons cultures were transferred to B5 basal medium supplemented with gibberellic acid and indole-3-butyric acid at different concentrations. The most effective treatment was B5 medium containing 14.4 µmol gibberellic acid plus 1.0 µmol indole-3-butyric acid in which 42.8 % of lobular structures cultured formed normal somatic embryos. High conversion of embryos into plantlets (61.0–65.2 % embryos regenerated plants) was observed when germinated embryos were placed on plant development medium.

Ograniczanie wyników

Evaluation of Streptomyces spp. against Fusarium oxysporum f. sp. ciceris for the management of chickpea wilt

Induced mutagenesis in Cicer arietinum

Expansion in chickpea (Cicer arietinum L.) seed during soaking and cooking

Appearance of different phosphatase forms and phosphorus partitioning in nodules of chickpea [Cicer arietinum L.] during development

Fungi isolated from chickpea [Cicer arietinum L.] seeds

Effect of the biological and simulated moisture contents of chickpea seeds [Cicer arietinum L.] on their physical properties

The role of 24-epibrassinolide induced antioxidant defence during germination under salinity in chickpea

Serological and molecular detection of bean leaf roll and chickpea chlorotic stunt luteoviruses in chickpea from Iran

Growth promotion and charcoal rot management in chickpea by Trichoderma harzianum

The effect of locality and sowing term on chosen morphological features of two chickpea (Cicer arietinum L.) cultivars

Nutritional variation and drought tolerance in chickpeas (Cicer arietinum L.)