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Expression of genes modulated by epigallocatechin-3-gallate in breast cancer cells

100%

Bogacz A., Wolek M., Juskowiak B., Karasiewicz M., Kaminski A., Uzar I., Polaszewska A., Kostrzewa Z., Czerny B.

Herba Polonica

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2018

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tom 64

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nr 3

Breast cancer is the most common malignant cancer among women. Both drug resistance and metastasis are major problems in the treatment of breast cancer. Therefore, adjuvant therapy may improve patients’ survival and affect their quality of life. It is suggested that epigallocatechin gallate (EGCG) which is well known for its chemopreventive activity and acts on numerous molecular targets may inhibit the growth and metastasis of some cancers. Hence, discovering the metastatic molecular mechanisms for breast cancer may be useful for therapy.The aim of the study was to determine the effect of EGGC on the mRNA expression level of genes such as ZEB1, ABCB1, MDM2, TWIST1 and PTEN in MCF-7 breast cancer cells. MCF7/DOX were cultured in the presence of 0.2 μM DOX and EGCG (20-50 μM). The mRNA expression level was determined by real-time quantitative PCR using RealTime ready Custom Panel 96 kit. Our results showed an important increase (about 2-fold for 20 μM EGCG + 0.2 μM DOX and 2.5-fold for 50 μM EGCG + 0.2 μM DOX, p<0.05) in ZEB1 expression levels. In case of ABCB1 gene lack of influence on the mRNA level was observed (p>0.05). We also observed significant decrease of ZEB1 expression in MCF7 cells with 20 μM and 50 μM EGCG (p<0.05). In addition, EGCG (20 μM) caused an increase of MDM2 and PTEN mRNA levels in almost 100% (p<0.05) and 40% (p>0.05), respectively. Lack of the influence of EGCG was noted for the TWIST1 gene expression. In case of MCF7/DOX we showed an increase of mRNA level of PTEN gene about 50% (p<0.05). These results suggest that EGCG may be potentially used in adjuvant therapy in the breast cancer treatment.

2

A correlation between the expression of nucleolar organizer regions [AgNORs] and some clinico-pathological features of sigmorectal adenocarcinomas

100%

Famulski W., Zalewski B., Sulkowski S., Miller-Famulska D., Sulkowska M.

Folia Histochemica et Cytobiologica

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1999

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tom 37

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nr 2

3

Liposomes loaded with 5-fluorocytosine and their possible application to suicide gene therapy of cancer cells modified with the bacterial cytosine deaminase gene

100%

Chaszczewska-Markowska M., Stebelska K., Grzybek M., Sikorski A. F., Ugorski M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

Detection of circulating breast cancer cells in peripheral blood by a two-marker reverse transcriptase-polymerase chain reaction assay

100%

Fabisiewicz A., Kulik J., Kober P., Brewczynska E., Pienkowski T., Siedlecki J. A.

Acta Biochimica Polonica

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2004

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tom 51

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nr 3

The aim of this study was to use a two-marker assay for the detection of breast cancer cells circulating in patients' blood. We have applied a PCR-based methodology to follow up the possibility of the development of metastatic disease in stage I and II patients who had undergone curative surgery. Since the number of circulating cancer cells in peripheral blood is very low, the technique for their detection needs to be not only highly sensitive, but also very specific. The reverse transcriptase-polymerase chain reaction (RT-PCR) technique may improve the sensitivity of breast cancer cell detection up to only a few cells per one million. The principle of the RT-PCR assay is to amplify a messenger RNA characteristic for breast epithelial cells in a blood sample. Since we do not expect such cells to be circulating in peripheral blood of healthy subjects, detection of the characteristic mRNA should indicate the presence of circulating breast cancer cells. We analyzed the usefulness of three mRNA markers: cytokeratin 19 (CK19), mammaglobin (hMAM) and S subunit of human chorionic gonadotropin (S-hCG) for this test. Blood samples (112) were obtained from 55 patients, in stages I and II, with or without metastasis to regional lymph nodes (N0 or N1). We found that a two-marker assay increases the sensitivity of detection of breast cancer cells in comparison with a single-marker one. Combination of two tumor-specific mRNA markers, hMAM/CK19 or S-hCG/CK19, allowed the detection of circulating breast cancer cells in 65% of N1 patients and 38% of N0 patients. By comparison, the combination hMAM/-hCG allowed the detection of circulating breast cancer cells in the blood of 68% of N1 patients and 46% of N0 patients. Addition of the third marker did not significantly increase the detection sensitivity.

5

Ascorbic acid inhibits the migration of Walker 256 carcinosarcoma cells

86%

Wybieralska E., Koza M., Sroka J., Czyz J., Madeja Z.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 1

103-111

The results of several experimental studies have shown that ascorbic acid inhibits tumor growth and metastasis. Ascorbic acid is an antioxidant that acts as a scavenger for a wide range of reactive oxygen species (ROS). Both tumour metastasis and cell migration have been correlated with the intracellular ROS level, so it was postulated that the inhibitory effect of ascorbic acid derivatives on cell motility may be caused by scavenging of ROS. Time-lapse analyses of Walker 256 carcinosarcoma cell migration showed that both the speed of movement and the cell displacement were inhibited by ascorbic acid applied in concentrations ranging from 10 to 250 μM. This effect correlated with a reduction in the intracellular ROS level in WC 256 cells, suggesting that ROS scavenging may be a mechanism responsible for the inhibition of WC 256 cell migration. However, another potent antioxidant, N-acetyl-L-cysteine, also efficiently decreased the intracellular ROS level in WC 256 cells, but did not affect the migration of the investigated cells. These results demonstrate that intact, unmodified ascorbic acid applied in physiologically relevant and nontoxicconcentrations exerts an inhibitory effect on the migration of WC 256 carcinosarcoma cells, and that this may be one of the factors responsible for the anti-metastatic activity of vitamin C. However, our data does not support the hypothesis that the scavenging of intracellular ROS is the main mechanism in the inhibition of cancer cell migration by ascorbic acid.

6

Impact of roscovitine, a selective CDK inhibitor, on cancer cells: bi-functionality increases its therapeutic potential

86%

Wesierska-Gadek J., Brzoza A., Komina O., Maurer M.

Acta Biochimica Polonica

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2009

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tom 56

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nr 3

495-501

Increased expression and activity of proteins driving cell cycle progression as well as inactivation of endogenous inhibitors of cyclin-dependent kinases (CDKs) enhance the proliferative potential of cells. Escape of cells during malignant transformation from the proper cell cycle control rendering them independent from growth factors provides rationale for therapeutic targeting of CDKs. Exposure of rapidly growing human MCF-7 breast cancer and HeLa cervix cancer cells to roscovitine (ROSC), a selective inhibitor of CDKs, inhibits their proliferation by induction of cell cycle arrest and/or apoptosis. The outcome strongly depends on the intrinsic traits of the tumor cells, on their cell cycle status prior to the onset of treatment and also on ROSC concentration. At lower dose ROSC primarily inhibits the cell cycle-related CDKs resulting in a strong cell cycle arrest. Interestingly, ROSC arrests asynchronously growing cells at the G2/M transition irrespective of the status of their restriction checkpoint. However, the exposure of cancer cells synchronized after serum starvation in the late G1 phase results in a transient G1 arrest only in cells displaying the intact G1/S checkpoint. At higher dosage ROSC triggers apoptosis. In HeLa cells inhibition of the activity of CDK7 and, in consequence, that of RNA polymerase II is a major event that facilitates the initiation of caspase-dependent apoptosis. In contrast, in the caspase-3-deficient MCF-7 breast cancer cells ROSC induces apoptosis by a p53-dependent pathway. HIPK2-mediated activation of the p53 transcription factor by phosphorylation at Ser46 results in upregulation of p53AIP1 protein. This protein after de novo synthesis and translocation into the mitochondria promotes depolarization of the mitochondrial membrane.

7

The expression of protein kinase B in gastric cancer cell apoptosis induced 12-O-tetradecanoylphorbol-1,3-acetate

86%

Zhang B., Xia C.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 3

466-480

Protein kinase B (PKB/Akt) is a serine-threonine kinase functioning downstream of phosphatidylinositol 3-kinase (PI-3 kinase) in response to mitogen or growth factor stimulation. In several cell types, it plays an important anti-apoptotic role. TPA is a potent regulator of the growth of many different cell types. Here, we detected that TPA could induce cell apoptosis in the gastric cancer cell line, BGC-823. We also found that TPA inhibited the expression of PKB/Akt in a TPA concentration- and time-dependent manner. Furthermore, TPA inhibited the phosphorylation of PKB at Ser473, but did not affect the phosphorylation of Thr308. It only attenuated the expression of PKB/Akt and the phosphorylation of Ser473 in the cell nucleus, whereas it did not change the PKB/Akt distribution in BGC-823 cells. These results suggest that PKB/Akt inhibition by TPA may be the important factor in the mechanism of effect of TPA on gastric cell lines.

8

Effect of extracts from Inonotus obliquus on the growth and enzyme activities of HeLa 83 cancer cells

86%

Rzymowska J., Malarczyk E., Jarosz A.

Applied Biology Communications. Lublin Scientific Center

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1991

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tom 01

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nr 3

9

The alcohol dehydrogenase isoenzyme (ADH IV) as a candidate tumour marker of esophageal cancer

86%

Jelski W., Laniewska-Dunaj M., Niklinski J., Kozlowski M., Laudanski J., Szmitkowski M.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

Objective: Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are present in esophageal cancer cells. Moreover the total activity of ADH as well as the activity of class IV ADH isoenzyme is significantly higher in cancer tissue than in healthy mucosa. The activity of these enzymes in cancer cells is reflected in the sera and could thus be helpful for diagnostics of esophageal cancer. The aim of this study was to investigate a potential significance of ADH isoenzymes and ALDH as tumour markers of esophageal cancer. We defined diagnostic sensitivity, specificity, predictive value for positive and negative results, and receiver-operating characteristics (ROC) curve for tested enzymes. Methods: Serum samples were taken for routine biochemical investigation from 180 patients with esophageal cancer before treatment. Total ADH activity was measured by a photometric method with p-nitrosodimethylaniline as a substrate and ALDH activity by a fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I and II isoenzymes we employed the fluorometric methods, with class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was measured by a photometric method with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. Results: There was a significant increase in the activity of class IV of ADH isoenzyme (7.65 mU/l vs 5.88 mU/l) and total ADH activity (1198 mU/l vs 848 mU/l) in the sera of esophageal cancer patients compared to the control. The diagnostic sensitivity for ADH IV was 72%, the specificity 76%, the positive and negative predictive values were 80% and 72% respectively. The area under the ROC curve for ADH IV was 0.65. Conclusion: The results suggest a potential significance of ADH IV as a marker of esophageal cancer.

10

The intrabody targeting of hTERT attenuates the immortality of cancer cells

86%

Zhu X., Yang N., Cai J., Yang G., Liang S., Ren D.

Cellular and Molecular Biology Letters

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2010

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tom 15

|

nr 1

32-45

hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

11

STING signaling in cancer cells: important or not?

86%

Sokolowska O., Nowis D.

Archivum Immunologiae et Therapiae Experimentalis

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2018

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tom 66

|

nr 2

12

Effect of lectin from Chelidonium majus L. on normal and cancer cells in culture

86%

Fik E., Wolun-Cholewa M., Kistowska M., Warchol J. B., Gozdzicka-Jozefiak A.

Folia Histochemica et Cytobiologica

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2001

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tom 39

|

nr 2

13

Effective tumor immunotherapy: start the engine, release the brakes, step on the gas pedal, ... and get ready to face autoimmunity

86%

Tirapu I., Mazzolini G., Rodriguez-Calvillo M., Arina A., Palencia B., Gabari I., Melero I.

Archivum Immunologiae et Therapiae Experimentalis

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2002

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tom 50

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nr 1

14

The nitroxides pirolin and pirolid protect the plasma membrane of rat cardiomiocytes against damage induced by anthracyclines

86%

Koceva-Chyla A., Sokal A., Kania K., Gwozdzinski K., Jozwiak Z.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

15

The genotoxicity of amsacrine in normal cells and cancer cells

86%

Blasiak J., Gloc E.

Cellular and Molecular Biology Letters

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2002

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tom 07

|

nr Suppl.

16

Proliferating activity, changes of DNAS ploidy of lung cancer cells before and after chemotherapy

86%

Mroz R. M., Chyczewska E., Holownia A., Izycki T., Chyczewski L., Braszko J. J.

Folia Histochemica et Cytobiologica. Supplement

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2001

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tom 39

|

nr 1

17

Multidrug resistance reversal in cancer cells and in pathogenic microorganisms

86%

Michalak K., Hendrich A. B., Wesolowska O., Pola A., Lania-Pietrzak B.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

18

Endocrine disruptor contaminants in water and their adverse effects in humans

72%

Wesierska-Gadek J.

International Journal of Ecohydrology and Hydrobiology

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2006

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tom 06

|

nr 1-4

233-242

19

The effect of tyrosine kinase inhibitors, tyrphostins: AG1024 and SU1498, on autocrine growth of prostate cancer cells [DU145]

72%

Kisielewska J., Ligeza J., Klein A.

Folia Histochemica et Cytobiologica

|

2008

|

tom 46

|

nr 2

185-191

20

Androgen receptors as a prognostic and predictive factor in breast cancer

72%

Agrawal A. K., Jelen M., Grzebieniak Z., Zukrowski P., Rudnicki J., Nienartowicz E.

Folia Histochemica et Cytobiologica

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2008

|

tom 46

|

nr 3

269-276

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