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The conducted study was aimed at determining the level of contamination of broiler chicken carcasses by Campylobacter sp. at selected stages of post-slaughter processing: after defeathering, evisceration, washing, and chilling. Swabs were collected from a 20 cm² skin surface from the neck and cloacal areas and from the wall of the body cavity. The swabs were fixed in flat-bottom flasks with 20 ml of sterile diluent. The resultant suspension was inoculated into two parallel Petri dishes with selective culture media: Karmali agar and CCDA agar. The analyses demonstrated that during the processing, Campylobacter sp. was disseminated on the surface of broiler chicken carcasses. The highest contamination of the carcasses, reaching on average log 1.71 cfu/cm² was observed after their evisceration, especially on skin in the neck area - log 1.96 cfu/cm². In the washed carcasses, the cell count of Campylobacter decreased to a value of log 0.45 cfu/cm². A similar degree of contamination, i.e. log 0.38 cfu/cm², was recorded after chilling.
Infections with Campylobacter spp. occur as a result of consumption of live cells with food. In developing countries those infections are immensely common, particularly during early childhood and 5 to 10 cases can appear during the initial two years of life. The symptoms appear usually after 1-7 days from infection depending on the number of ingested cells and individual sensitivity. Characteristic symptoms of infections caused by Campylobacter spp. infrequently occurring jointly in the clinical form of the disease include: diarrhea, abdominal pain and increased temperature. In the majority of cases the disease is mild and lasts from 2 to 7 days. Usually Campylobacter are excreted with feces during a period of 7-21 days, sometimes even longer. Occasionally in the increased risk group dangerous complications may occur. They include: bacteremia, meningo-myelitis, neurological disturbances and reactive arthritis.
The occurrence of Campylobacter in poultry meat and subsequent antibiotic resistance profiles of the isolates were investigated. The prevalence of Campylobacter in 101 samples was 87.1%. Of these samples, 54.5% contained Campylobacter coli and 45.5% were contaminated with Campylobacter jejuni. Among the strains tested, resistance to ciprofloxacin and nalidixid acid was the most common, followed by tetracycline and streptomycin. On the other hand, all analysed isolates were susceptible to erythromycin and gentamycin. Moreover, the prevalence of several virulence marker genes among Campylobacter isolates was estimated. All strains showed the presence of the flaA and cadF factors, whereas the iam was identified only in C. jejuni, while the cdtA, cdtB, and cdtC genes were amplified almost in all C. coli isolates.
This study analysed the pattern of antibiotic resistance in 251 Campylobacter strains isolated from symptomatic children hospitalized in 4 large paediatric hospitals in Poland from 2000 through 2007. The highest resistance was found for ciprofloxacin (49.5% for C. jejuni and 51.3% for C. coli), followed by tetracycline (17.5% and 18.0%, respectively), and ampicillin (13.2% and 10.2%, respectively). Almost all isolates were susceptible to macrolides. As much as 22.6% of C. jejuni and 25.6% of C. coli were resistant to more than one class of antimicrobial agents. Multidrug resistance (defined as resistance to at least two classes of antimicrobials) rose significantly from 5.1% in 2000-2003 to 34.6% in 2004-2007.
The main aim of this study was to evaluate the prevalence of campylobacteriosis in population of Polish heifers. An additional objective was to compare two independent methods used in diagnosis of the disease: culture and PCR. The 1,600 vaginal swabs from heifer origin from different farms of Poland were investigated using both methods. The results showed that the percentage of positive samples was 0.75% when the culture method was used, while the application of PCR method has shown 1.81% positive results.
Forty-three C.f.s.f. strains and 19 C. jejuni strains from miscarried sheep foetuses and rectal mucosa smears were isolated. The C.f.s.f. strains were used to prepare 4 vaccines. The dynamics of the occurrence of antibodies against anti-Campylobacter in the sheep immunized with these vaccines was similar. The highest mean titres were found from experimental weeks 8 to 10. The vaccine applied in disease focus revealed satisfactory protective properties. The results obtained made it possible to select the strains which can be used for immunization of sheep.
Although vaccination, after having been more than 200 years in medical practice, has proven to be the most effective and the cheapest way to prevent infectious diseases, they remain still the main cause of human premature deaths. As many pathogens enter the human body through the mucosal surfaces, the mucosal way of immunization is considered to be the most promising strategy to decrease the number of human infections. Moreover, the oral delivery system eliminates the necessity of injection what is extremely important for pediatric immunization programs. However, most of recently constructed subunit vaccines based on purified bacterial/viral antigens are rather poorly immunogenic. This review presents some novel ways to enhance and modulate host immune responses by combining antigens with specific adjuvants or by employing specific delivery systems. We also discuss some recent technologies, based on mining the genomic sequences of bacterial pathogens, which accelarate and improve identification of new candidates for vaccine construction. As an example, we focus on the progress in the development of vaccine against Campylobacter spp. Campylobacter jejuni is now recognized as a leading cause of bacterial enteritis in human.
Przedstawiono pierwsze w kraju badania nad występowaniem Campylobacter spp. w przewodzie pokarmowym lisów polarnych. Spośród 108 przebadanych lisów Campylobacter spp. wyizolowano w kale 9 lisów (8,3%,), Stwierdzono występowanie Campylobacter spp. u 8,2% lisów w fermie państwowej arm u 8,5% w fermach prywatnych. Z badanych ferm prywatnych 2 byty wolne od Campylobacter spp. Lisy polarne mogą być bezobjawowymi nosicielami Campylobacter species i stanowić rezerwuar i źródło zakażenia kampylobakteriozą. Izolowane szczepy byly wrażliwe na kwas nalidyksynowy, cloxacyklinę (75% szczepów), nitrofurantoins (50%), streptomycynę, doxycyklinę, gentamycynę i sulfonamidy (25%); oporne wobec penicyliny, oxytetracykliny, chloramfenikolu, i kolistyny.
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