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The effects of iv infusion of 7.2% NaCl (HSS, 4 ml/kg; n=6) or 7.2% NaCl in 6% dextran 70 (HSD, 4 ml/kg; n=6) followed by administration of 0.9% NaCl (ISS, 32 ml/kg) on sodium (Na), chloride (Cl), potassium (K) serum concentrations, osmolality (Osm), total protein content (TP), packed cell volume (PCV), haemoglobin concentration (Hb) and red blood cell count (RBC) were evaluated in twelve healthy sheep for 360 min. The changes in blood parametres after infusion of hypertonic and isotonic solutions were not significantly different between HSS and HSD groups. In these groups the increase in Na, Cl and Osm accompanied by the decrease in K, TP, PCV, Hb and RBC were observed at 5 min after administration of hypertonic solutions. Infusion of ISS 60 min after administration of HSS or HSD did not produce a further increase in Na, Cl and Osm, whereas K, TP, PCV, Hb and RBC decreased to the lowest values at 90 min. These results indicate that the initial bolus dose of HSS or HSD produces a rapid recruitment of extravascular fluids into the vascular compartment, whereas subsequent large volume infusion of ISS maintains intravascular blood volume expansion and does not create a risk of hypernatremia.
The aim of these investigations was to determine the influence of garlic powder additives on gains, feed intake and conversion, and some biochemical blood parameters of piglets. The investigations were carried out on 419 piglets from 36 sows, assigned randomly to 6 groups. The piglets of group I received the basal diets without antimicrobial growth promoters (AGP) and group II received the basal diets with AGP additive. The animals of experimental groups (III VI) received the same diets as group I, but supplemented with lyophilized garlic powder in doses of 1.0, 2.0, 5.0 or 10.0 g per kg diet in groups III VI, respectively. Piglets were weaned at 28 days of age. From the second week of age until 28th day after weaning, the piglets were fed a commercial Prestarter mixture ad libitum. Body weight was measured individually at birth, at the weaning period, and at the end of the trial (56th day of life). Feed intake by piglets was recorded weekly for litters in each pen. Blood samples were collected at weaning (28 th day) and on day 56 after birth, and contents of protein, glucose, total cholesterol, HDL-cholesterol and blood urea nitrogen were determined. The garlic bulbs and garlic powder were analyzed for alliin and allicin concentrations. Powder from lyophilized garlic clover additive, especially in 5 or 10 g/kg mixtures, may constitute a recommended dietary component for raising piglets up to 56 days of life. This procedure, an alternative for AGP use, improves body weight gains, decreases piglet losses as well as triglycerides and total cholesterol concentration in blood plasma.
In 230 mice from 12 inbred strains (A.CA/W, AKR/W, BALB/cW, BN/aW, CBA/W, CBA-T6/W,C3H/W, C57BL/6W, C57BL/10W, DBA2/W, 129S1/SvW, HLB219/J) the following parameters were measured: the total number of white (WBC) and red (RBC) blood cells, haemoglobin concentration (HGB), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and platelets (PLT) number. Highly significant interstrain differences were shown within RBC, HGB, HCT, MCV and PLT.
Background. Arsenic is widely distributed in the environment. The main routes of absorption of inorganic arsenic compounds are the lungs and the gastrointestinal tract. Arsenates both (III) and (V) are absorbed from the gastrointestinal tract in 55-95%, while the organic arsenic compounds in 75-85%. Arsenic poisoning leads to damage the activities and morphological changes in the stomach and intestines, causing the occurrence of nausea, vomiting and diarrhoea. Arsenic compounds may also be the cause of the development of certain cancers (lung, skin and liver). The first changes caused by arsenic poisoning usually remain unnoticed. Arsenic affects haematological and both lipid and carbohydrate metabolism. It also causes changes in the organs involved in metabolism, so biochemical parameters or enzymes activity are therefore a good indicator of poisoning changes. Objective. The aim of this study was to examine the influence of protein and fat content in diet on selected biochemical blood parameters in rats. Materials and Methods. Rats (11 groups n = 88) were fed with 5 types of diet: control, low-protein, high-protein, low- fat and high-fat. Animals received water without arsenic (control group) or water with 10 or 20 μg As/mL. Results. In animals fed a low protein diets, regardless of the dose of arsenic, it was a decreasing of total cholesterol, triglycerides, and glucose in serum observed, compared to the control group. In the groups fed with low-protein diet revealed a significantly less damage in the liver as compared to the control group. In animals fed high-protein diets and with varying addition of arsenic a significant higher concentration of various biochemical parameters were found, in comparison to the respective control groups. In animals fed the high protein diet and poisoned with 20 μg As/mL of the arsenic significantly higher liver damage were found, compared to control group. Conclusions. Symptoms of arsenic hepatotoxicity measured with enzyme activity were highest in the groups of animals fed with low-protein diet. The parameters of lipid and carbohydrate metabolism depended mostly on diet than the dose of arsenic.
On dairy farms with high milk production there are much health problems. One of the most important periods, the critical time in dairy cows production is calving. In controlling and resolving of these problems the great role have the farmer and the veterinarian. There are many varied methods of dairy farms supervising. Diagnostic monitoring in herds of dairy cattle has an important role in controls an acceptable level of health and productivity in the cows and their progeny. Being familiar with the internal environment of the animal enables early detection of any dangers to the health of the animal and enables one to react before any clinical signs appear. Thus management in periparturient dairy cows is very important for prevention of many disturbances. The aim of the study was to compare the values of selected blood parameters in dairy cows shortly pre and post partum. It had to answer on the question The materials consisted of 30 clinical healthy cows tested 3-7 days before calving and 3-5 days after calving in this same good environmental and nutritional conditions (borning room). Selected twenty one various biochemical and morphological parameters of the blood similar to standard metabolic profile were included in the study. Significant differences (α=0.05) were noted in total bilirubin, SGOT, inorganic phosphorus, Calcium and Chloride. Parturition is an important period in cows from the point of view of the physiological changes taking place which in turn produce measurable, significant changes in the diagnostic parameters of the blood. These differences are presented in the paper.
Influence of lactation stage on selected blood parameters and biological value of cow milk during pasture season in organic system of production. The disproportion between the cow's genetically determined ability to produce milk and the limits to improving the energy value of feed may be the cause of the metabolic stress of animals kept at organic farms. Negative energy balance has a major impact on the body's hormonal balance and the function of the organs; it also affects the content of basic components (protein, fat and lactose) in cows' milk. The aim of the study was to investigate changes in the biological value of organic milk obtained from high producing dairy cows at different stages of lactation during pasture season. The study was carried out on 68 Polish Holstein-Friesian primiparous. 272 milk samples were collected from cows which were in one of the following phases: the beginning of lactation (BL): 5-30 days postpartum); peak of lactation (PL): 60-90 days postpartum; full lactation (FL): 120-150 days postpartum); end/final phase of lactation (EL): 250 days postpartum. Stage of lactation had an overriding effect on the majority of analyzed milk constituents including: whey proteins, vitamins soluble in fat, phospholipids and fatty acids. The highest levels of lactoferin were found at the beginning of lactation (BL). At the same time, the β-hydroxybutyrate (BHBA) concentration increased, which confirmed that the fast growing milk yield at the peak of lactation is linked to an increase in energy demand. The lowest alanine aminotransferase and non esterified fatty acids activity was measured during BL. The study has shown a significant influence of lactation stage on the biological value of organic milk obtained from high producing dairy cows during pasture season. In addition, there is evidence that the organic production system has no influence on the metabolic profile of cows as the plasma parameters were within their standard norms. This might be the result of the positive effect of grass antioxidants.
The aim of study was to investigate the effect of yeast cell walls (mannanoligosaccharides - MOS) supplemented to a sow's diet on some blood parameters of sows and their piglets. The investigations were carried out in two experiments with two groups of 30 sows each. The sows of groups 1 and 3 (control) received the basal diet, groups 2 and 4 (MOS) - the same diets, supplemented with yeast cell walls preparation. The sow basal diet in experiment I contained 40% of wheat and in experiment II - 46% of triticale. The level of MOS supplementation (8 g per sow daily) was based on the manufacturer's recommendations. A total of 782 piglets were received. The piglets were weighed at birth and on days 14 and 28 of age. The health status of the piglets, their mortality rate from birth until weaning, and diarrhoea incidences were recorded daily. Blood plasma contents of total protein, uric acid, urea, selected mineral elements (Fe, Cu, Zn), total cholesterol, triacyloglycerol, HDL cholesterol, and LDL cholesterol, blood plasma activities of catalase (CAT), superoxide dismutase (SOD), aminotransferase alanine, aminotransferase aspartate, dehydrogenase lactate, phosphatase alkaline, and the level of ferric reducing ability of plasma (FRAP) were determined. The addition of MOS to sows' diet increased the values of FRAP and contents of Fe and Cu in sows, and increased the activity of SOD and CAT, level of FRAP, and contents of urea and Fe and decreased the concentration of cholesterol and LDL-cholesterol in piglets. The receiving MOS supplement. The obtained results showed that the used indicators of the antioxidant system especially SOD, CAT, and FRAP, are very sensitive exponents of antioxidant status of swine. Species of grain (wheat or triticale) did not have any significant influence on the analysed sows' and piglets' blood parameters.
The aim of study was to investigate pre-partum and postpartum changes in the total anti-oxidant capacity (TAC), total peroxide (TPX) and oxidative stress index (OSI) of cows with dystocia compared with healthy animals. The possible relationships between these markers and relevant blood parameters were also investigated. Examinations were performed in a herd of 200 Holstein cows, aged 3-6 years. Blood samples were taken both during the 7th month of pregnancy and within the first 15 minutes postpartum. After calving, the cows were divided into two groups according to the type of birth: Group 1 dystocia (n=16) and (Group 2) normal partum (n=21). Additionally, cows in the dystocia group were divided into three subgroups, according to the reason for the dystocia: absolute birth weight (the high birth weight of calf), twin pregnancy and presentation. The results indicated that there were no changes in oxidative stress either in dystocia or during normal parturition eutoci. Oxidative stress may be decreased using an antioxidant supplement during the pre-partum period. The study also showed that oxidative stress does not affect dystocia. It may also be concluded that additional antioxidant supplementation may be required in absolute birth weight and twin pregnancy to reduce TPX levels. Analyses of urea, uric acid, and creatinine, as well as the enzyme activities of lactate dehydrogenase, alanine and aspartate transferase pre-partum do not appear to be useful for predicting dystocia. However, decreasing serum total protein may be a parameter to be used in diagnosing pathological conditions in pregnancy - particularly during late pregnancy.
The current methods of preventing calcium deficiency during the periparturient period are based primarily on a diet low in calcium and acidifying the rumen content before parturition, as well as on oral administration of liquid preparations or boluses. The available literature does not provide information on whether these forms of prevention are effective only immediately after the parturition, or whether they also affect an organism throughout the initial lactation period. The aim of the study was to examine the effects of three methods for the prevention of postpartum hypocalcaemia on selected indicators of mineral and energy metabolism, as well as on functional parameters of the parenchymal organs during the two week period following parturition in dairy cows diagnosed with low calcium concentrations before parturition, induced by a lower dietary mineral content. The study was conducted in 60 HF cows, aged 3-6 years old, fed TMR. The animals were divided into three groups: the first group received only a mineral preparation contained in the feed, adjusted to the physiological stage and milk production level; the second group received three additional doses (directly before the parturition, then 24 hours and 48 hours after the delivery) of an oral fluid preparation containing 62.5 g of Ca; while the third group received an intraruminal bolus containing 43 g of pure calcium (immediately after parturition). This research showed that physiological hypocalcaemia occurred at the beginning of lactation in the groups which had received additional calcium preparations (groups II and III), whereas in group I blood collection one week and two weeks after the parturition demonstrated subclinical hypocalcaemia. A statistically significant increase in magnesium and a decrease in phosphorus concentrations were also observed in the latter group, as well as an excessive GGTP activity and high bilirubin concentrations. Using only one complex mineral supplement in high yielding milk cows after parturition does not ensure the expected prophylactic effects, especially in the long term.
The comparative study was performed on 200 European brown hares. Group I consisted of 35 clinically healthy hares kept in cages. Group II comprised 165 clinically healthy hares caught alive in their natural environment. Following the premedication, arterial and venous blood was drawn from the hares of the two groups studied. In the arterial blood, the acid-base balance (ABB) parameters were determined - blood pH, pCO₂, t CO₂, HCO₃⁻, BB, and BE. In the venous blood, the activity of AST, ALT, and FA, concentration of urea, creatinine, total protein, albumins, globulins, cholesterol, triglycerides, glucose, WBC, RBC, Hb, and Ht were determined. In addition, concentrations of Ca²⁺, P, Mg²⁺, Na⁺, K⁺, and Cl⁻ were measured in the blood serum. It was demonstrated that only the contents of globulins and triglycerides were similar in groups I and II. Concentration of electrolytes and ABB parameters were close to each other in the two studied groups, except for concentration of Mg²⁺ and inorganic P, and CO₂ molecular pressure. In the light of the obtained results a question remains opened: whether successful breeding, understood as an increase in the number of offspring of the reintroduced individuals, is more likely to occur in the case of animals caught alive and, adapted to living in their natural environment, or in the case of caged animals.
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