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This paper describes laparoscopy-guided prostate biopsy experiments in 13 dogs. Biopsy material was evaluated histopathologically to diagnose causes of prostatic gland enlargement. Laparoscopic biopsy was performed in male dogs diagnosed with prostatic gland enlargement, based on clinical symptoms and results of rectal examinations.
In chronic lymphocytic leukaemia (CLL) bone marrow trephine biopsy (BMT) is not required for diagnosis but can have a significant prognostic value and can be used for the detection of the minimal residual disease (MRD) and for assessment of the effectiveness of the treatment applied. The aim of the study was to evaluate the morphological changes in bone marrow after treatment with purine nucleoside analogues cladribine and fludarabine. Bone marrow trephine biopsy was taken routinely from 15 patients with CLL. Bone marrow trephine biopsy was performed on every patient before as well as after chemotherapy. The number of cell elements of the marrow (the degree of atrophy), the patterns of bone marrow infiltration, the presence of reticulin and collagen fibres and the disturbances in bone marrow stroma were assessed. The infiltration of bone marrow by neoplastic cells was observed in all the patients before administration of chemotherapy. The infiltration was followed by an increase in the number of reticulin fibres. After the treatment a regression of the reticulin fibres was observed with the lessening of the infiltration. After the treatment the levels of marrow infiltrate were decreased. Increased hypoplasia of the bone marrow was observed after the chemotherapy.
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Cytopathology of canine lymphomas (100 cases)

84%
Malignant lymphoma is one of the most common malignant tumours occurring in dogs. Fine needle aspiration biopsy (FNAB) is an excellent, specific diagnostic procedure used to assess pathological processes in lymph nodes. The aim of the present study was to conduct a cytopathological analysis of lymphoma in dogs and to analyse some epidemic aspects of occurrence of lymphoma in 100 dogs using Giemsa stained slides. Samples were obtained by fine needle aspiration biopsy, fine needle non-aspiration biopsy, lymph node impression smears and by examination of body cavity effusions. The determination of the type and subtype of tumour was made on the basis of the updated Kiel classification adopted for dogs. Based on cytopathological analysis, the lymphoma was diagnosed in 100 dogs: 44 were female and 56 male. The animals’ age ranged from 1.5 year to 15 years (median: 7.5 years), the animals were of different breeds (72% of all dogs belonged to 28 different breeds) and crossbreeds (28%). In 29% of dogs the regional or general lymphadenomegaly was the only clinical sign observed, in remaining cases (71%) at least one abnormality connected to lymphoma was found. Among all diagnosed lymphomas, high-grade lymphomas were more prevalent (86% of all cases) than low-grade lymphomas (14% of all cases). The possibility of boxers having a predisposition to T cell lymphoma development could be also suspected.
The aim of this study was to determine the influence of two different media on the viability of in vitro produced biopsied bovine morulae. Bovine morulae were produced in vitro, then biopsied and cultured in the Ham's F10 and IVM media. Cultured and control morulae were stained with Hoechst 33342 and propidium iodide. Morulae were classified morphologically for excellent, good and degenerated quality. 42.86% of biopsied morulae cultured in the Ham's F10 medium and only 11.11% (only one) of these embryos cultured in the IVM were of excellent quality. Embryos of good quality were about 2 times less numerous in Ham's F10 medium (28.57%) than in IVM medium (55.5%) (P < 0.05). 28.57% of biopsied morulae cultured in Ham's F10 medium and 33.33% of these embryos cultured in the IVM degenerated (P > 0.05). The media had no significant influence on the number of total and viable blastomeres of morulae cultured in vitro after biopsy (P > 0.05). But the quantity of restored (excellent and good quality) embryos was higher when they were cultured after biopsy in Ham's F10 medium than in IVM. These statistically significant results (P < 0.05) show that the Ham's F10 medium is better for the restoring of biopsied bovine embryos produced in vitro than IVM.
Helicobacter pylori in gastric mucosa biopsy specimens may be detected by means of several methods like histo-pathological assessment, culture on bacteriological media and urease tests. Comparative evaluation of the two most popular in Poland urease tests („Test Ureazowy" and CLOtest) and bacterial culture on solid medium has been carried out. The sensitivity, specificity and utilitarian properties of these diagnostic methods were analyzed on the ground of the results obtained after examination of biopsies taken from 100 patients referred to upper gastroscopy. The rate of color reaction in urease tests was also estimated in relation to the number of Hp cells and other urease producers introduced to reaction well. The superiority of the "Test Ureazowy" over CLOtest and culture is postulated in conclusion.
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67%
The aim of this study was to evaluate the utility of immunocytochemistry in a standard veterinary practice and to determine the immunophenotype of tumor cells in cases of multicentric lymphoma in dogs by immunocytochemical analysis of fine-needle biopsy specimens. The study was performed on cytological samples collected from 54 dogs, in which multicentric lymphoma was recognised based on clinical data, cytology or cytology and histology, and follow-up information. Diagnosis of lymphoma was established according to the updated Kiel classification. Immunocytochemical assays were conducted using commercially available antibodies to the pan T-lymphocyte marker CD3 and B cell antigen receptor complex CD79 alpha. Among all animals examined B cell lymphoma was recognized in 42/54 (77.8%) of cases, while in the remaining 12/54 (22.2%) of dogs T cell lymphoma was recognized. In 11 animals with lymphoma recognized cytologically, in which an entire lymph node was obtained for histology, the results of routine cytology and immunocytochemistry fully corresponded with findings revealed by histology and immunohistochemistry. Immunocytochemistry can be successfully conducted in smears stored at room temperature for 24 hours without changes of staining results. It can be stated that application of standard cytopathological assessment in connection with immunocytochemistry of lymph nodes samples collected from dogs with lymphoma is a method of choice for establishing final diagnosis, and avoids the need for reexamination or collection of tissue samples for histopathology and immunohistochemistry during surgical procedures in ambiguous cases.
The objective of this study was to evaluate the usefulness of the laparoscopic technique for performing a biopsy of the gastric and duodenal wall in dogs. Endoscopic examinations were performed in five dogs, which were not conclusively diagnosed based on symptoms and the results of clinical and laboratory tests. A histopathological analysis of laparoscopically collected tissue samples supported the diagnosis of a chronic inflammation of the gastric and duodenal wall. The results of the experiment indicate that laparoscopy permits accurate mapping of the biopsy site and supports the collection of tissue samples for histopathological analyses.
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