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Рассмотрено альтернативное постиндустриальное направление решения проблем сельскохозяйственного производства за счет интегрированного использования биотехнологических альтернатив. Предложена методология разработки сельскохозяйственных технологий по типу биоконверсного комплекса. Разработаны вариант системного подхода и алгоритм проектирования сельско-хозяйственных биоконверсных комплексов, позволившие устранить недостатки существующего эвристических методов.
The biotransformation of (–)–menthol by Mucor ramannianus was studied. It was carried out with sporulated surface cultures of Mucor ramannianus. The main bioconversion products obtained from (–)–menthol were trans-p-menthan-8-ol, trans-menth-2-en-1-ol, sabinane, pmenthane- 3,8-diol, isomenthol, and 1,8-cineole, also resulting in higher yields. Biotransformation with sporulated surface cultures was also monitored in Petri dishes and the same solid medium, Sabouraud Dextrose Agar (SD A), was used. In the solid agar medium inoculated with spores of Mucor ramannianus, first germination of the spores and then mycelial growth took place. After 1 week, the surfaces of Petri dishes were covered with spores and biotransformation reaction had started. However, there is no report on the biotransformation of (-)-menthol using Mucor ramannianus. Six isolates (93.6%) found Mucor ramannianus as a biocatalyst and biotransformation of (–)–menthol was investigated. The pathways involved in the biotransformation of (–)–menthol by two main products are also discussed.
 Thymol is present in the essential oils from herbs and spices, such as thyme. It is produced by these plant species as a chemical defense against phytopathogenic microorganisms. Therefore, this compound has attracted great attention in food industry, i.e., it has been used as a natural preservative in foods such as cheese to prevent fungal growth. Previous studies concerning the biotransformation of nerol by Penicillium sp. and microbial transformation of citral by sporulated surface cultures method (SSCM) of Penicillium digitatum have been reported. The objective of this research was to study the pathway involved during biotransformation of citral by Penicillium sp. using two methods. The culture preparation was done using different microbial methods and incubation periods to obtain Penicillium for citral biotransformation. The biotransformation products were identified by gas chromatography (GC) and gas chromatography/mass spectroscopy (GC/MS). A comparison of the two methods showed that SSCM was more effective, its major products were thymol (21.5 %), geranial (18.6 %) and nerol (13.7 %). LM produced only one compound - thymol - with a low efficiency.
A simple and efficient method of carrying out biotransformation reactions on terpenoid compounds was developed. For these experiments, a sporulated surface culture of Penicillium sp. was inoculated on solid media in conical flasks. After a short incubation the spores germinated and a mycelia culture was formed. After a week the cultures had completely sporulated and a bioconversion reaction started. For this purpose, a known volume of menthol was added onto the sporulated surface of culture. After 7 days, a period during which transformation took place, menthol was extracted with Et2O three times. After evaporation the recognition by GC and GC/MS was followed. The main bioconverted products obtained from menthol by surface Penicillium sp. with the use of sporulated surface culture were α-pinene (18.0%), sabinene (11.6%), trans-p-menthan-1-ol (10.6%), p-menth-1-ene (5.8%), 1,8-cineole (6.4%) and limonene (3.2%). The pathways of biotransformation of menthol by Penicillium sp. to main products are also discussed.
Microbial conversions are gaining importance in the synthesis of important drug metabolites and their intermediates as they are good alternative to chemical synthesis since they are enantio-selective and regio-selective and even can be carried out at ambient temperature and atmospheric pressure. Till date, biocatalytic reduction of acetophenone and its derivatives has been widely reported. In the present study, we have made an attempt to carry out the microbial bioreduction of o-hydroxyacetophenone by screening some of the selected microorganisms which were obtained from culture collection centre as well as those which are isolated in our Microbiology lab. The selected microorganisms include Aspergillus ochraceous, Aspergillus flavus, Aspergillus tubingenesis, Aspergillus niger, Rhizopus stolanifer MTCC 162, Rhizopus stolanifer MTCC 2591 and Baker’s yeast.Among the seven microorganisms screened for the bioreduction of o-hydroxyacetophenone, Baker’s yeast and Aspergillus tubingenesis showed significant bioconversion where as Aspergillus ochraceous exhibited the least bioconversion.In our earlier study it was found that Aspergillus flavus has the required bioreductase enzyme, which showed the maximum conversion of p-chloroacetophenone to p-chlorophenylethanol. Hence optimization of culture conditions to get maximum enzyme expression and hence maximum conversion was thought off. The parameters considered for the study include effect of various Carbon sources, Nitrogen source, Metal ions, incubation Temperature and media pH on enzyme expression. The optimized culture a condition at which maximum bioconversion was achieved was maltose among various carbon sources. Tryptone was found to have maximum effect among the nitrogen sources. Media pH 7.6 and incubation temperature of 35 °C was found to be favourable for maximum enzyme activity. Among various divalent metal salts, addition of magnesium sulphate to the media significantly increased the enzyme activity.
Zaolejona ziemia bieląca (Z) jest odpadem porafinacyjnym przemysłu tłuszczowego o naturalnym kwaśnym pH 3,8-4,2 i zawartości tłuszczu 18-40%. W pracy zbadano aktywność lipolityczną autochtonicznego szczepu Bacillus subtilis BH w biodegradacji substancji tłuszczowych Z w zależności od stymulacji wybranymi dodatkami mineralnymi (jonami wapnia i/lub żelaza). Zastosowanie tego szczepu w warunkach in situ z udziałem aktywatorów enzymów lipolitycznych prowadziło do powstawania różnych związków chemicznych, takich jak: kwasy nasycone, aldehydy i węglowodory, a ich rodzaj i ilość zależały od typu modyfikacji. W obecności szczepionki obserwowano wzrost zawartości węglowodorów w produktach rozkładu substancji tłuszczowej. Natomiast jony wapnia stymulowały jej aktywność w kierunku tworzenia aldehydów ogółem, a jony żelaza - nasyconych kwasów tłuszczowych.
Growth characteristics, gross composition and the nutritive value in terms of liver protein regeneration capacity, protein efficiency ratio and the protein digestibility of the biomass of Kluyveromyces marxiamis subsp. marxianus NRRL Y-2415 were determined after growth in concentrated acid whey (12.72% lactose) at 30°C. The biomass (11.2 g/L) had a crude protein content of 49.8% and true protein content of 42.4%. Groups of 5 male albino rats 21-28 days old when given diets with protein supplied by casein, protein biomass from Kluyveromyces marxianus subsp. marxianus NRRL Y-2415 or the protein biomass supplemented with 0.5% DL-methionine showed liver protein regeneration capacity (after a 2-day fast) of 90%, 34% and 93.3%, protein efficiency ratio 2.6, 1.76 and 2.83 and true protein digestibility of 93.30, 93.31 and 93.43%, respectively. Feeding of rats with single cell protein showed no ill effects on the rats.
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