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Taxol (paclitaxel) is a chemotherapeutic diterpene with promising anticancer activity that blocks cell division by preventing microtubule depolymerization. Furthermore, recent studies have shown that taxol has other intracellular effects that may contribute to its effect, particularly in macrophages. The signal transduction mechanisms by which taxol stimulates macrophages to anticancer activity are not clear. The purpose of this study was to determine the effect of taxol on chemiluminescence (an indicator of the production of free radicals) of neutrophils, macrophages and murine macrophage J.774.2 cells. The chemiluminescence was measured in the presence of taxol andór phorbol myristate acetate (PMA) as a stimulant. Taxol stimulated chemiluminescence (without PMA) of neutrophils and macrophages but not of J.774.2 cells, and modulated chemiluminescence of the cells stimulated with PMA.
The paper presents the results of the research on Bryoria capillaris (Ach.) Brodo & D. Hawksw. in the Polish part of the Białowieża Forest. Two new sites of this species have been found in the managed Białowieża Forest, in Władysław Szafer Reserve. Bryoria capillaris grows on the bark and small branches of Quercus robur and Picea abies.
Larval galleries and exit holes of adult insects in wood and bark are a conspicuous indication of the occurrence of many species. Therefore it allows the qualitative and quantitative examination of insect populations. However, evaluation of adult body size based on the size and shape of galleries and exit holes was not practiced. We analyzed relationship between selected biometric traits (pronotal width, elytral width, and length of elytron) of adult Rosalia alpina (L.) − an endangered representative of the longhorn beetles (Cerambycidae) − and the size and shape of their exit holes. Based on the laboratory reared individuals, we evaluate the size and shape of exit hole of each adult. Adult biometric traits were strongly correlated with each other. No sexual differences were found in adult beetle size and exit hole size and shape. The size of exit holes was, however, a good indicator of adult body size. The correlation between exit hole size and the size of adult R. alpina was highly significant for all studied traits, whereas exit hole shape was weakly correlated with adult size. The results of this study show new possibilities for applying the size of adult exit holes to study and wider characterize populations of R. alpina, which includes e.g. inter-population, inter-habitat, or inter-host material comparisons and determination of adult body size changes over time-period.
The influence of an adsorbent bed height made of tree bark on ammonia emission reduction was investigated under two scenarios: for constant mass of adsorbent and for adsorbent mass as a function of bed height. In both cases, measurements were performed on adsorptive beds of comparable height; other experimental conditions are presented in the part of the paper dealing with research methodology. The choice of tree bark as adsorbent material has been dictated by the results of preliminary research that was conducted on other biodegradable adsorbents in order to fulfill the basic assumption, i.e. that such materials, saturated with ammonia, should serve as natural organic fertilizer in plant production. The amount of ammonia adsorbed on tree bark increased linearly with the increasing height of the adsorbent bed. These results were analyzed together with the data on reduced ammonia emissions, including the amount of adsorbed pollution and bed height. Moreover, the ratio of bed height to the adsorber inner diameter (h/d w) was used in data analysis. For the given optimal height of an adsorbent bed, the most appropriate time for replacing tree bark is when breakthrough first occurs.
In the late 18th and early 19th c., a drastic reduction in the area of forests due to their excessive exploitation occurred in Europe, which gave rise to the birth of afforestation efforts. The chemical composition of plants, including trees, depends on the many biotic and abiotic environmental factors. The aim of this study was to determine the content of some nutrients in needles and bark of Scots pine (Pinus sylvestris L.), leaves and bark of silver birch (Betula pendula) and Norway maple (Acer platanoides L.), depending on their location. The content of phosphorus, potassium, sodium, calcium and magnesium depended on a plant species, plant organ and the location of sample collection. The leaves of silver birch, compared to its bark, contained ten-fold more potassium and six-fold more phosphorus and magnesium. The content of phosphorus was the highest in silver birch leaves in the center of Olsztyn. The highest potassium content was observed in silver birch leaves and Norway maple bark in the center of Olsztyn. The calcium content was the highest in the leaves and bark of maple trees growing near State Road 51 and in the leaves of this species in the city center. The highest sodium content was detected in the leaves and bark of most tree species growing along State Road 51 and in the center of Olsztyn.
The paper reports results of investigations concerning the influence of saponin extract of quillaja on the food choice and development of Colorado potato beetle. Choice tests with the use of potato leaf discs treated and untreated with tested saponins’ extract were made. The influence of the presence of saponins on the food choice and intensity of insect feeding were observed. It was stated that saponins significantly restrain feeding and disturb development of this pest
The present work was conducted to investigate antibacterial activity of methanol and acetone in leaf (LE) and stem-bark (SBE) of Ficus sycomorus L. crude extracts against sensitive and resistant species of Staphylococcus aureus and Acinetobacter baumannii pathogens. Antimicrobial activity expressed by disc-diffusion method (zone of inhibitions – ZIs), minimum inhibitory concentrations (MICs) and minimum bactericidal concentration (MBC) were measured as reported for many investigations. Similar study with 6 commercial antibiotics as a reference drug was undertaken. Based upon the estimated ZIs, MIC and MBC values, acetone LE exhibited higher antimicrobial activity than that of methanol one. Otherwise, standard antibiotics have lower effectiveness (ZIs, MICs and MBC) on all tested bacteria as compared to the SBE and LE. The highest antibacterial activity was recorded in sensitive A. baumannii isolate with MICs 2.5, 4.9 mg/ml and MBC 3.8, 9.7 mg/ml for acetone LE and SBE, respectively. Our data indicated that the lowest antibiotics antibacterial activity was recorded for resistant A. baumannii pathogen. It was lower than those of the both plant fractions extracts.
Randia dumetorum (family Rubiaceae) is highly reputed ayurvedic medicinal tree commonly known as the Mainphal. A large deciduous thorny shrub grows up to 5 m of height. It occurs almost throughout India up to 1200 m of altitude. It is found in Himalaya from Jammu East ward ascending to 400 m and from Kashmir to East ward up to 1200 m. 11-methylixoside (compound 1), an iridoid glucoside, was isolated from the bark of this plant. The structure was characterized by using spectroscopic methods including 1D-1HNMR,13C-NMR and 2D-NMR (HSQC,HMBC, DQF-COSY) experiments and confirmed by comparison of their NMR data with those from the literature. This compound has been reported for the first time in Randia dumetorum bark. The 11-methylixoside was subjected to cytotoxic activity against MDA-MB-231 (breast cancer cell line) and SK-MEL-2 (human skin melanoma cell line), BE(2)C (neuroblastoma cell line derived from human bone marrow) and U87MG (human neuronale glioblastoma (astrozytom) cell line showed appreciable cytotoxic effect with IC50 value 63.10 µg/ml concentration for SK-MEL-2 (human skin melanoma cell line).
The experiment was carried out at the Department of Ornamental Plants in the Agricultural University in Lublin in the years 2000–2002. The plant material was corms of Acidanthera bicolor var. murielae Perry with a circumference of 8–9 cm, which in number of thirty was planted on a plot 1.2 m², at 8 cm of depth in the second week of May. According to the scheme of experiment: linuron 1.000 kg·ha⁻¹, pendimethalin 0.990 kg·ha⁻¹ and napropamide 1.500 kg·ha⁻¹ were applied one week after planting the corms of the research plant. Next day some plots were mulched with 5 cm thick layer of pine bark or peat. The experiment included also a control set without herbicides and mulches, which it was hand weeded and a control set with delay hand weed performed three week late compared with the rest plots. At the end of vegetative stage the measurement was carried out on 10 plants of each plot including: length of flower stem, number of flowers and length of spike. It was noted that linuron, pendimethalin and napropamide did not injury to plants of Acidanthera bicolor. Application of linuron and pendimethalin beneficially influenced on number of flowers (respectively by 9.65 and 9.72 pieces·plant⁻¹), length of flower stem (respectively by 102.54 and 101.52 cm) and length of spike (respectively by 40.14 and 40.28 cm). Besides application of napropamide had a positive effect on received of greater number of flowers (9.40 pieces·plant⁻¹) and longer spike (39.60 cm). Delay of hand weed about three weeks had a negative effect on yield and quality of flowers of the research plant which formed the smaller number of flowers and the shortest flower stems and spikes (respectively by 8.4 pieces·plant⁻¹, 96.2 cm and 35.4 cm). It was received the longer flower stems in combinations where applied of pine bark or peat as a mulch (respectively by 100.88 and 100.23 cm) in comparison with combination without mulch (97.96 cm). There was no noted considerably influence of organic mulches on number of flowers in spike and length of inflorescence.
Pinus sylvestris L. seedlings grown for 12-17 months in pure culture after inoculation with S and P strains of Heterobasidion annosum (Fr.) Bref. were studied with the help of light and transmission electron microscopy. Standardized inoculation procedure and uniform in vitro conditions allowed evaluation of the host/pathogen interactions. Necrotic reaction of the bark cells associated with the accumulation of dark-stained material in vacuoles, and lignification of their walls in root regions in proximity to mycelium were the common host reaction. Hypertrophy of the bark cells in areas invaded by hyphae caused a tension that was responsible for strong deformation of the bark cells. Injury to the vascular cambium resulted in formation of barrier zone parenchyma and abnormal xylem elements. High amount of polyphenolic material accumulated in new formed parenchyma cells was an effective chemical “barrier” to the centripetal spread of hyphae. Resin-plugged tracheids and row parenchyma cells rich in polyphenolic material were common in wood areas colonized by the fungus.
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