Wyniki wyszukiwania - AGRO

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Investigations of the properties of probiotic Lactobacillus plantarum U-14 and Lactobacillus fermentum U-5 strains and their evaluation during the lyophilization

100%

Oberauskas V., Kantautaite J., Sutkeviciene R., Sederevicius A., Monkeviciene I., Zelvyte R., Ramanauskiene J., Laugalis J., Kabasinskiene A.

Medycyna Weterynaryjna

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2004

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tom 60

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nr 12

1278-1282

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Changes in microbial dehydrogenase activity and pH during bioremediation of fuel contaminated soil

86%

Wieczorek D., Marchut-Mikolajczyk O., Antczak T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 4

3

Reduction of biogenic amines in cheese using selected lactic acid bacterial strains and high hydrostatic pressure

86%

Pasztor-Huszar K., Simon P., Dalmadi I., Kisko G., Simon-Sarkadi L.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

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nr Suppl.1

4

Crystalline protein profiling and cry gene detection in Bacillus thuringiensis strains isolated during epizootics in Cydia pomonella L.

86%

Konecka E., Baranek J., Kaznowski A.

Biological Letters

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2014

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tom 51

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nr 2

5

Bio-transformation of selenium in Se-enriched bacterial strains of Lactobacillus casei

86%

Kurek E., Ruszczynska A., Wojciechowski M., Luciuk A., Michalska-Kacymirow M., Motyl I., Bulska E.

Roczniki Państwowego Zakładu Higieny

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2016

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tom 67

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nr 3

Background. Selenium is an element of very great importance for the proper functioning of the human body, mainly due to its antioxidant properties. Selenium exhibits a preventive effect in the case of cardiovascular disease, the immune system, male infertility and inhibits the toxic action of other agents. Selenium is important for Hashimoto’s disease. Intake of selenium in the diet slows the aging process. The biological and toxicological effects of selenium strongly depend on its chemical form. Some organisms for example: plant, yeast, are capable of metabolizing low bioavailable selenium compounds (inorganic selenium) into its high bioavailable forms (organic selenium). Objective. The aim of this study was to investigate the bio-transformation of selenium by Lactobacillus bacteria towards the characterisation of selenium metabolites. Material and Methods. The speciation of selenium was evaluated by high performance liquid chromatography with inductively coupled plasma mass spectrometry detector. The extraction of selenium species from lyophilized bacteria was executed with water, the mixture of lipase and protease, as well as lisozyme and sodium dodecyl sulphate. Results. All investigated bacteria strains cultivated in the presence of Na2SeO3 effectively uptake selenium. Surprisingly, none of the applied extraction media exhibited a strong power to release the majority of the uptaken selenium compounds. Thus a maximum of 10% of the selenium was extracted from bacteria exposed to the enzymes. However, it was found that Lactobacillus bacteria are able to metabolize inorganic ions of selenium (IV) into Se-methionine, Se-methyloselenocysteine and other unidentified forms. Conclusions. The study confirmed the ability of probiotic bacteria to biotransform inorganic selenium into its organic derivatives. Therefore, Se-enriched bacteria can be considered as an addition to the functional food.

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Cytotoxic activity of Serratia marcescens clinical isolates

86%

Krzyminska S., Raczkowska M., Kaznowski A.

Polish Journal of Microbiology

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2010

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tom 59

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nr 3

Twenty Serratia marcescens isolates from clinical specimens were examined for their cytotoxic activity on four cell lines (HEp-2, Vero, CHO, J774). Most of the isolates were found to be cytotoxic to CHO (70%), Vero (75%) and HEp-2 cells (90%). CHO cells were the most sensitive to cell-free supematants, followed by HEp-2 and Vero cells. Two strains produced cytotonic toxins which caused elongation of CHO cells. Moreover, twelve isolates (60%) revealed cytotoxic potential to macrophage cell line J774. The results indicate that these bacteria may destroy phagocytes and epithelial cells, which may lead to spread within the host.

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Comparative study of PCR-based approaches for the genetic characterization of three strains of Acidithiobacillus caldus isolated from different sites in China

86%

Wu X., Duan H., Fan H., Zhang Z., Liu L.

Polish Journal of Microbiology

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2013

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tom 62

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nr 4

Comparative study of the genetic characteristics among three Acidithiobacillus caldus strains isolated from different typical environments in China was performed using a combination of molecular methods, namely sequencing analysis of PCR-amplified 16S rRNA genes and 16S-23S rRNA gene intergenic spacers (ITS), repetitive element PCR (rep-PCR), arbitrarily primed PCR (AP-PCR) fingerprinting and random amplified polymorphic DNA (RAPD). Both of the 16S rRNA gene and 16S-23S rRNA gene intergenic spacers sequences of the three strains exhibited small variations, with 99.9-100%, 99.7-100% identity respectively. In contrast, according to the analysis of bacterial diversity based on rep-PCR and AP-PCR fingerprinting, they produced highly discriminatory banding patterns, and the similarity values between them varied from 61.97% to 71.64%. RAPD analysis showed that banding profiles of their genomic DNA exhibited obvious differences from each other with 53.44-75% similarity. These results suggested that in contrast to 16S rRNA genes and 16S-23S rRNA gene intergenic spacers sequencing analysis, rep-PCR, AP-PCR fingerprinting and RAPD analysis possessed higher discriminatory power in identifying these closely related strains. And they could be used as rapid and highly discriminatory typing techniques in studying bacterial diversity, especially in differentiating bacteria within Acidithiobacillus caldus.

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Immobilized cells of recombinant Escherichia coli strain for continuous production of L-aspartic acid

86%

Szymanska G., Sobierajski B., Chmiel A.

Polish Journal of Microbiology

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2011

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tom 60

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nr 2

For L-aspartic acid biosynthesis, high production cells of Escherichia coli mutant B-715 and P1 were immobilized in chitosan gel using a technique developed in our laboratory. The immobilization process reduced initial activity of the intact cells, however, the biocatalyst produced was very stabile for long-term use in multi-repeated batch or continuous processes. Temperature influence on the conversion of ammonium fumarate to L-aspartic acid was investigated. In long-term experiments, over 603 hours, the temperature 40°C was found to be the best for both biocatalyst stability and high conversion rate. The optimum substrate concentration was 1.0 M. Continuous production of L-aspartic acid was investigated in three types of column bioreactors characterized by different volumes as well as different high to biocatalyst bed volume rations (Hz/Vz). The highest conversion rate, 99.8%, and the productivity 6 g/g/h (mass of L-aspartic acid per dry mass of cells in biocatalyst per time unit) was achieved in the bioreactor with the highest value Hz/Vz = 3.1, and liquid hour space velocity value of 5.2, defined as the volume of feeding substrate passed per volume of catalyst in bioreactor per one hour.

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Antibacterial activity of Ulva fasciata against multidrug resistant bacterial strains

86%

Chandrasekaran M., Venkatesalu V., Raj G. A., Krishnamoorthy S.

International Letters of Natural Sciences

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2014

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tom 14

The present study was conducted to evaluate the antibacterial activity of different organic solvent increasing polarity viz., hexane, chloroform, ethyl acetate, acetone and methanol extracts of Ulva fasciata (Chlorophyceae) were collected from Kanniyakummari, Gulf of Mannar biosphere Reserve, Tamilnadu, India. Marine green algae extracts of U. fasciata against multi-drug resistant standard and clinical bacterial strains viz., Bacillus subtilis, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium, Vibrio cholerae, Shigella flexneri, Proteus mirabilis and P. vulgaris. The ethyl acetate extracts of U. fasciata showed highest antibacterial activity against all the bacterial strains tested. The mean zone of inhibition produced by the extracts in disc diffusion assays were ranged from 7.1 mm to 15.0 mm. The Minimum Inhibitory Concentrations (MIC) were between 125 μg/ml and 500 μg/ml, while the Minimum Bactericidal Concentrations (MBC) were between 250 μg/ml and 1000 μg/ml. The highest mean of zone inhibition (15.0 mm) and lowest MIC (125 μg/ml) and MBC (250 μg/ml) values were observed in ethyl acetate extract of U. fasciata against B. subtilis. The ethyl acetate extract of the U. fasciata showed the presence of phytochemicals, terpenoids, tannins and phenolic compounds in U. fasciata than the other solvents extracts. The present results of the ethyl acetate extract of U. fasciata can be used as an antibacterial substance for the treatment of multi drug resistant bacterial infections.

10

Response of bacteria to heavy metals measured as changes in FAME profiles

86%

Markowicz A., Plociniczak T., Piotrowska-Seget Z.

Polish Journal of Environmental Studies

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2010

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tom 19

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nr 5

The effects of Cd, Ni, Cu, or Zn on the whole cell-derived fatty acid profiles of four bacterial strains isolated from heavy metal-polluted soils located in Upper Silesia was determined. Based on the fatty acid methyl ester (FAME) profiles, the strains were identified and named as Enterobacter intermedius AM15, Enterobacter intermedius MH8b, Pseudomonas putida MH1d, and Klebsiella pneumoniae AM12. The obtained results showed changes that were dependent both on tested strains and metal used. The most significant changes were observed for strains cultured in the Ni presence. In the FAME profiles of MH8b, AM15, and AM12 strains, a significant increase of cyclopropane fatty acids was observed. Moreover, exposure for Ni resulted in the appearance of a new fatty acid in the FAME profiles of AM15 and MH8b strains. In turn, Cd and Zn caused a decrease of the content of cyclopropane fatty acids as compared to control. For AM15 and AM12 strains cultured on media with heavy metals, the ratio of saturated to unsaturated fatty acids were higher than that in control. The same phenomenon was also observed for MH8b strain exposed only to the highest concentration of Ni and Cd.

11

Activity of catabolic enzymes of film-forming strains of Staphylococcus aureus

86%

Voronkova O. S., Shevchenko T. M., Vinnikov A. I.

International Letters of Natural Sciences

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2017

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tom 61

The activity of glucose catabolism pathways of film-forming strains of staphylococci isolated from vagina of women with dysbiosis of reproductive tract and from women without disorders of microflora was studied. It was established that the investigated film-forming strains utilized the carbohydrates by pentose phosphate pathway mainly, as indicated by 23-33% higher enzyme activity compare to strains isolated from healthy women. Instead strains, isolated from women without dysbiosis of reproductive tract, have higher activity of glycolytic enzymes on 13- 28%. The prevalence of glycolytic transformation of glucose by strains isolated from healthy women also indicates by the depression of glucose oxidation during action of monoiodinacetate – classical inhibitor of glycolysis. It inhibits glycolysis of strains isolated from healthy women more significant. It was established that oxidase activity of film-forming strains isolated from women with dysbiosis, increased over 40% during the use of basic substrates of citric acid cycle. These data indicate a general increase of catabolic activity of oxidative type of staphylococci isolated during vaginal dysbiosis and able to form biofilm.

12

Technical aspects of random amplified polymorphic DNA [RAPD] technique in genotyping of bacterial strains

86%

Gzyl A., Augustynowicz E.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 3

13

Effect of DNA methylation on transient expression of PR-s genes in tobacco protoplasts

86%

Brodzik R., Hennig J.

Journal of Applied Genetics

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1996

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tom 37A

14

Instability of hybrid plasmids in Bacillus subtilis

86%

Wojcik K., Wieckiewicz J., Kuczma M., Porwit-Bobr Z.

Acta Microbiologica Polonica

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1993

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tom 42

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nr 2

15

Fluorescent microscopy and gas chromatography to assess the viability and metabolic activity of Propionibacterium sp. strains

72%

Warminsko-Radyko I., Sielawa H., Miks-Krajnik M.

Polish Journal of Food and Nutrition Sciences

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2010

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tom 60

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nr 4

The aim of the study was to assess usefulness of the LIVE/DEAD fluorescent staining method and gas chromatography to monitor the viability and metabolic activity of Propionibacterium strains in long-term cultures in milk. The effect of 4% NaCl addition and a temperature of 10°C on the growth of Propionibacterium freudenreichii ssp. freudenreichii 111, 109C, 108 strains was studied for 28 days. Bacterial cells were assessed in cultures by microscopic and plate counting methods in regular intervals. The cultures were additionally determined for the content of volatile fatty acids: C2 to C7. The total cell counts of all strains in cultures assessed by the microscopic method were noticed to be 1 to 5 logarithmic cycles higher in comparison to those determined with the plate counting method. In following days and weeks of culture, increasing discrepancies were observed between the results obtained using microscopic and plate methods. Both methods revealed similar trends in the viability of strains under control conditions and a little impact of NaCl addition on cell growth and decrease. The cultures run at a temperature of 10°C exhibited different course of growth and decline of the number of monitored populations depending on strain and method applied. Individual strains possessed different acid formation activity. From the beginning of incubation, the highest concentrations were reported for propionic and acetic acids, whereas the other acids in number from 4 to 6 appeared subsequently. The temperature of 10°C inhibited acids formation by all strains, whereas 4% addition of NaCl stimulated the acid-forming activity and during incubation under those conditions the contents of volatile acids were recorded to be the highest.

16

Susceptibility of Polish Yersinia enterocolitica strains isolated from pigs to 12 beta-lactam antibiotics

72%

Perkowska K., Platt-Samoraj A., Bancerz-Kisiel A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 3

The aim of the study was to assess the susceptibility of 103 Yersinia enterocolitica strains isolated from pigs in Poland in the years 2000-2007 to 12 ß-lactam antibiotics. The in vitro susceptibility of the bacteria to seven selected cephalosporins of all generations and to five penicillins was tested by means of a disk diffusion method following the criteria and recommendations provided by the National Committee for Clinical Laboratory Standards. The strains varied greatly in regard to their in vitro susceptibility- to ß-lactam antibiotics. The strains were found to be relatively highly susceptible to the third and fourth generation cephalosporins, while being generally resistant to the first generation cephalosporins and most penicillins. Taking into account the wide spread of Y. enterocolitica, in particular in the pig population, but also among other animal species, which creates an increasing risk to the public health, it is deemed necessary to systematically monitor the susceptibility of Y. enterocolitica strains to antibiotics.

17

The effect of plant growth promoting bacteria on transplants growth and lettuce yield in organic production

72%

Szczech M., Szafirowska A., Kowalczyk W., Szwejda-Grzybowska J., Wlodarek A., Maciorowski R.

Journal of Horticultural Research

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2016

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tom 24

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nr 2

18

Isolation, biotyping, and serotyping of Yersinia enterocolitica strains from fattening pigs

72%

Bancerz-Kisiel A., Szczerba-Turek A., Platt-Samoraj A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 1

The study has been taken up to collect more data on Yersinia enterocolitica isolated from pigs as the main reservoir and source of infection with strains pathogenic for humans. Bacteriological examinations, bio- and serotyping were conducted on 616 rectal swabs, taken from 308 fattening pigs. Two samples were taken from each animal to determine the ability of Y. enterocolitica to grow under different temperature conditions (warm and cold culture). It has been proven that low temperature constitutes a suitable culture condition. 138 Yersinia enterocolitica strains were isolated (22.40%), most of which (65.22%) were obtained in cold culture and 99.28% included in biotype 3 (one strain belonged to biotype 2). Serotyping yielded a positive result in 107 strains with the diagnostic serum for antigen O:3, in 18 - with the serum for antigen O:9, and 13 strains were determined to be non-typable. The results indicated that asymptomatic infections with Y. enterocolitica strains of the biotypes and serotypes pathogenic for humans are common in pig population.

19

Adherence of Pseudomonas aeruginosa strains to solid surfaces

72%

Wolska K., Sowka A., Bukowski K., Jakubczak A.

Acta Microbiologica Polonica

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2001

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tom 50

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nr 3-4

311-314

20

Echinacea purpurea stimulates cellular immunity and anti-bacterial defence independently of the strain of mice

72%

Bany J., Siwicki A. K., Zdanowska D., Sokolnicka I., Skopinska-Rozewska E., Kowalczyk M.

Polish Journal of Veterinary Sciences

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2003

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tom 06

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nr 3 Suppl.

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