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Babesjoza - trudnosci diagnostyczne

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Human babesiosis is caused predominantly by B. microti and B. divergens, a protozooan parasites of red blood cells. Both are transmitted by Ixodes ricinus ticks, also the primary vector of Lyme disease. Clinical manifestation varied widety from asymptomatic infection to a serve rapidly fatal disease. The diagnosis of babesiosis include examination of stained blood smers, serological evaluation indirect antibody tests and PCR. With the evolution PCR - based techniques, the diagnosis and monitoring of babesial infections became more sensitive and reliable.
Nucleic acid-based methods offer a variety of tools for the detection of parasites. This field of veterinary and medical sciences is rapidly evolving. Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid method whereby DNA is amplified with high specificity, efficiency, and rapidity under isothermal conditions using a set of four specifically designed primers. In this paper we present the usefulness of LAMP method in the diagnosis of babesiosis in various animal species. Babesia species-specific LAMP assay may have potential clinical application in the detection and differentiation of Babesia species, particularly in countries in which babesiosis is endemic. It can be assumed that in the near future this technique will be a routinely used diagnostic test in veterinary practices.
During the last several years Babesia canis infections have become one of the most serious parasitic diseases of dogs in Poland, often leading to the death of the animals. Acute babesiosis manifests itself with fever, lethargy, lost of appetite, haemoglobinuria, and vomiting. However, some of the infections may be asymptomatic or insufficient treatment may lead to chronic, asymptomatic babesiosis. In the present paper, the case of a 2-year-old dog is described. The dog needed to undergo splenectomy after a spleen rupture during spontaneous play with other dogs. The likely cause for the spleen rupture was splenomegaly observed during USG and surgery. Several hours after surgery, babesiosis symptoms were manifested, with haemoglobinuria, fever and typical blood picture, morphology and biochemistry. The short time from the surgery to the manifestation of symptoms and observed splenomegaly suggested asymptomatic B. canis infection as an indirect cause of the spleen rupture.
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Babeszjoza psow - wciaz aktualny problem

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Tick-borne dog infectious diseases constitute an emerging problem in veterinary medicine in recent years. The diseases recognized as characteristic for tropical and semi-tropical regions are diagnosed in dogs in countries with temperate climate including Poland. The major reason for such phenomenon is that Polish tourists increasingly travel with their dogs into subtropical and tropical areas of the world. Coming back to Poland, dogs often bring in non-native species of ticks as well as pathogens transmitted by them. Tick-borne diseases can result from infection with bacteria, viruses and parasitic protozoa. In this review we present most important tick-transmitted diseases which may occur in dogs in Poland.
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