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Changes in the ascorbate-glutathione system during storage of recalcitrant seeds of Acer saccharinum L.

100%

Ratajczak E., Pukacka S.

Acta Societatis Botanicorum Poloniae

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2006

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tom 75

|

nr 1

23-27

Two seed lots of Acer saccharinum (recalcitrant), with an initial moisture content of 50% and 55%, were stored at +3oC for 6 months. After this time, their viability (measured as germinability) reached 100% and 30%, respectively. In embryo axes and cotyledons extracted from seeds, two major low molecular antioxidants were assayed: ascorbate (ASA and DHA) and glutathione (GSH and GSSG); and activities of enzymes of the ascorbate-glutathione cycle were measured: ascorbate peroxidase (APO) (E.C. 1.11.1.11), monodehydroascorbate reductase (MR) (E.C. 1.6.5.4), dehydroascorbate reductase (DHAR) (E.C. 1.8.5.1), and glutathione reductase (GR) (E.C. 1.6.4.2.). GSH and GSSG contents of embryo axes of stored seeds decreased, as compared to the control (fresh, non-stored seeds), but a larger decrease was observed in seeds with 30% viability. In cotyledons, a particularly high increase in the GSH content in relation to the control was observed in seeds with 100% viability, while the GSSG content was significantly lower in both stored seed lots than in the control. The ASA level was twice as high in seeds with 30% viability as in the control, both in embryo axes and in cotyledons. The activity of enzymes of the ascorbate-glutathione cycle was higher in embryo axes than in cotyledons. In embryo axes of seeds with 100% viability, enzyme activities were slightly lower than in the control, while in those of seeds with 30% viability, their activities were higher than in the control. The observed changes in activities of enzymes of the ascorbate-glutathione cycle and in ascorbate and glutathione levels suggest that the stored seeds of A. saccharinum have an active antioxidant system, which plays an important role in maintaining their viability during storage.

2

Isolation and characterisation of a cDNA encoding a novel cytosolic ascorbate peroxidase from potato plants [Solanum tuberosum L.]

100%

Soares d. C. D., Pereira C. S., Teixeira J., Pereira S.

Acta Physiologiae Plantarum

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2006

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tom 28

|

nr 1

Ascorbate peroxidase (APX) is one of the key enzymes of the plant antioxidant system playing, along with catalase, a central role in hydrogen per oxidescavenging. An approach to further in crease the knowledge about cytosolic APX gene organization can be achieved by isolating and characterisating new cDNAs, thus providing new in sights about the physiological roles and regula tion of these enzymes. A partial cDNA clone (corresponding to the 3’ untranslated region), cytosolic ascorbate peroxidase-re lated, was isolated from potato sprouts by RT-PCR. Data base analysis re trieved several expressed sequence tags (ESTs) coding potato cytosolic ascorbate peroxidase, that were used to infer the complete cDNA sequence. The deduced amino acid sequence revealed high homologies with other plant cytosolic ascorbate peroxidases, con firming the reliability of the vir tual cDNA. Northern blot analysis revealed the existence of a single band related to the isolated cDNA and the southern blotting results al lowed the elaboration of a possible gene organization.

3

Bundle sheath-specific expression of the ascorbate peroxidase 2 affects photosynthesis in whole plant

84%

Gorecka M., Alvarez Fernandez R., Bialasek M., Mulleineaux, Karpinski S.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 2

4

Characteristics of the plant ascorbate peroxidase family

84%

Dabrowska G., Kata A., Goc A., Szechynska-Hebda M., Skrzypek E.

Acta Biologica Cracoviensia. Series Botanica

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2007

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tom 49

|

nr 1

7-17

This paper reviews plant ascorbate peroxidases (APX), an important part of the antioxidative system, maintaining the balance and uninterrupted functioning of the plant cell. The main role of APXs is to control the hydrogen peroxide concentration in cells. In reaction the enzymes use ascorbate as an electron donor. The active site is highly conserved by every member of the APX family. APXs belong to class I of the superfamily of bacterial, fungal and plant peroxidases. All the isoforms differ from each other in molecular weight, optimal pH, stability, substrate specificity, localization and level of response to specific stress conditions. It is suggested, however, that the responsible genes originated from one common gene by multiple duplication events followed by natural selection.

5

Effect of ethylene antagonist silver thiosulphate on the flower longevity of Clarkia pulchella Pursh.

84%

Dar R. A., Tahir I.

Journal of Horticultural Research

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2018

|

tom 26

|

nr 1

6

The properties of peroxisomal ascorbate peroxidase from sugar beet (Beta vulgaris ) and expressed in E. coli

84%

Kurnik K., Skorupa-Klaput M., Tyburski J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

7

Examining organic acid root exudate content and function for leafy vegetables under water-stressed conditions

84%

Kasukawa N., Miyazawa K.

Journal of Horticultural Research

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2020

|

tom 28

|

nr 2

8

Effect of nanosilver on physiological performance of pelargonium plants exposed to dark storage

84%

Hatami M., Ghorbanpour M.

Journal of Horticultural Research

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2013

|

tom 21

|

nr 1

9

The role of ascorbate during cadmium-induced oxidative stress in Arabidopsis thaliana

84%

Bielen A., Remans T., Vangronsveld J., Cuypers A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 2

10

Functional analysis of peroxiredoxin isoforms in Euglena gracilis

84%

Tamaki S., Maruta T., Sawa Y., Shigeoka S., Ishikawa T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 2

11

Antioxidant defence system in the leaves and roots of A. thaliana under long-term S deficiency

84%

Ostaszewska M., Juszczuk I. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 3

12

The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, Apis mellifera during two different seasons

84%

Korayem A. M., Khodairy M. M., Abdel-Aal A.-A. A., El-Sonbaty A. A. M.

Journal of Biology and Earth Sciences

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2012

|

tom 02

|

nr 2

13

Dual regulation of cytosolic ascorbate peroxide (APX) by tyrosine nitration and S-nitrosylation

84%

Begara-Morales J., Sanchez-Calvo B., Chaki M., Valderrama R., Mata-Perez C., Lopez-Jaramillo J., Carreras A., Padilla M., Corpas F., Barroso J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

|

tom 94

|

nr 2

14

Effect of temperature on oxidative stress induced by lead in the leaves of Plantago major L.

84%

Balakhnina T. I., Borkowska A., Nosalewicz M., Nosalewicz A., Wlodarczyk T. M., Kosobryukhov A. A., Fomina I. R.

International Agrophysics

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2016

|

tom 30

|

nr 3

15

Induction of enzymatic scavengers of active oxygen species in rice in response to infection by Rhizoctonia solani

84%

Paranidharan V., Palaniswami A., Vidhyasekaran P., Velazhahan R.

Acta Physiologiae Plantarum

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2003

|

tom 25

|

nr 1

16

Engineering stress tolerance in transgenic plants: the role of antioxidants and prooxidants

84%

Allen R. D.

Biological Bulletin of Poznań. Supplement

|

1997

|

tom 34

17

Changes in the pattern of antioxidant enzymes in wheat exposed to water deficit and rewatering

84%

Feng Z., Jin-Kui G., Ying-Li Y., Wen-Liang H., Li-Xin Z.

Acta Physiologiae Plantarum

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2004

|

tom 26

|

nr 3

18

Effect of two different ambient ozone concentrations on antioxidative enzymes in leaves of two tobacco cultivars with contrasting ozone sensitivity

67%

Bandurska H., Borowiak K., Miara M.

Acta Biologica Cracoviensia. Series Botanica

|

2009

|

tom 51

|

nr 2

37-44

Eight-week-old tobacco (Nicotiana tabacum L.) Bel W3 (ozone sensitive) and Bel B (ozone resistant) cultivars were exposed to ozone for two weeks at two sites with differing tropospheric ozone levels in five independent series from May 27 to July 25, 2004. After each exposition, the degree of ozone-caused visible leaf damage and the activity of APX, GuPX, and SOD were examined. Visible leaf damage was observed only in the sensitive cultivar; the resistant one did not exhibit any external symptoms. Three-way ANOVA revealed that the activity of all enzymes varied by exposure site, series and cultivar effects. Significant correlations between GuPX activity in the two cultivars and with the degree of leaf damage to the sensitive cultivar were found. This indicates that GuPX activity in the sensitive as well as in the resistant cultivars track changes in tropospheric ozone levels. The positive correlation between ozone level and APX activity in the resistant cv. Bel B, which did not reveal visible symptoms, indicates that this enzyme may contribute to detoxication of H2O2 and alleviation of oxidative damage caused by O3

19

The effect of multiple heavy metals on ascorbate, glutathione and related enzymes in two mangrove plant seedlings [Kandelia candel and Bruguiera gymnorrhiza]

67%

Huang G.-Y., Wang Y.-S., Sun C.-C., Dong J.-D., Sun Z.-X.

Oceanological and Hydrobiological Studies

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2010

|

tom 39

|

nr 1

11-25

20

The effect of sugars on in vitro growth of Leucojum aestivum L. plants

67%

Ptak A., Moranska E., Simlat M., Skrzypek E., Warchol M., Nowak M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

Ograniczanie wyników

Changes in the ascorbate-glutathione system during storage of recalcitrant seeds of Acer saccharinum L.

Isolation and characterisation of a cDNA encoding a novel cytosolic ascorbate peroxidase from potato plants [Solanum tuberosum L.]

Bundle sheath-specific expression of the ascorbate peroxidase 2 affects photosynthesis in whole plant

Characteristics of the plant ascorbate peroxidase family

Effect of ethylene antagonist silver thiosulphate on the flower longevity of Clarkia pulchella Pursh.

The properties of peroxisomal ascorbate peroxidase from sugar beet (Beta vulgaris ) and expressed in E. coli

Examining organic acid root exudate content and function for leafy vegetables under water-stressed conditions

Effect of nanosilver on physiological performance of pelargonium plants exposed to dark storage

The role of ascorbate during cadmium-induced oxidative stress in Arabidopsis thaliana

Functional analysis of peroxiredoxin isoforms in Euglena gracilis

Antioxidant defence system in the leaves and roots of A. thaliana under long-term S deficiency

The protective strategy of antioxidant enzymes against hydrogen peroxide in honey bee, Apis mellifera during two different seasons

Dual regulation of cytosolic ascorbate peroxide (APX) by tyrosine nitration and S-nitrosylation

Effect of temperature on oxidative stress induced by lead in the leaves of Plantago major L.

Induction of enzymatic scavengers of active oxygen species in rice in response to infection by Rhizoctonia solani