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The postpartal period in high-yielding dairy cows can be characterized as a time when animal has to undergo several important changes affecting all the organism. After pregnancy and parturition, organism has to adapt itself to the lactation and this stressing experience contributes to the high incidence of health disorders. These include ketosis, fatty liver, milk fever, retained placenta, metritis, mastitis, laminitis, and other metabolic and reproductive disorders. They may become clinically manifested in later stages of the reproduction cycle but have their primary cause in early postpartal period. To overcome these problems, every farmer has to practice good herd health management, especially when considering the transition period. To discover the weak points of the herd management we can use some indices that can be determined from the blood samples of dairy cows after partuition. We collected blood samples from 170 dairy cows of Slovak spotted breed 1 to 8 weeks after parturition. None of them manifested clinical signs of any illness. We divided them into the 6 groups depending on the number of weeks post partum.We observed and evaluated the main blood-chemistry parameters of energy and liver metabolism (glukose, triglycerides, total lipids, total cholesterol, non-esterified fatty acids, beta-hydroxybutyrate, total bilirubin), protein metabolism (total immunoglobulins, total protein, albumine, Creatinin, urea) and levels of some serum enzymes (AST, GGT, ALP, CPK, LDH). Even though there were no alterations in the health status present in that time, we found out that some indices, mostly of energy and liver metabolism, such as serum levels of non-esterified fatty acids, triglycerids, etc. were altered. This fact can help us to trace the potentially threatened individuals and to iniciate the steps that would minimize further damage.
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The material consisted of 60 hybrid boars, from line 990 (female) and Pietrain breeds (male), divided into two groups depending on the litter size in which they were born and reared - group: 7-10 piglets (average 8.8) per litter; group: 11-17 piglets (average 12.5) per litter. The mean body weight of 21 day of live piglets born and reared in larger litters was significantly (P < 0.05) lower than among boars reared in smaller litters. This difference gradually diminished and at day 63 of life both groups had the same average body weight. In the period from 63 to 180 day of life, the boars from smaller litters had a slightly lower rate of growth (statistically insignificant). Boars reared in larger litters at 180 day of life were on average 3 kg heavier. There were no significant differences in fat thickness, lean meat and testicular volume between the assessed groups of young boars. From boars reared in smaller litters ejaculates had a significantly higher (P < 0.05) sperm concentration and total sperm volume.
The aim of the study was to present carpal flexural deformity cases in dogs, which are seldom reported in literature, from the clinical and biochemical point of view. Carpal flexural deformity was determined in a total of 31 puppies, of which 28 were brought to our clinic and 3 reported by a practicing veterinary surgeon. The puppy's ages ranged between 6-24 weeks, and they belonged to 10 different breeds. Following clinical and radiological examination of the puppies, blood samples were taken and calcium, phosphorus and magnesium values were recorded. Slight increases in these mineral values were determined in some of the patients. A splint with a caudal aluminum support, padded with a large amount of cotton, was applied to all puppies with deformity. This splint was kept on for 10 days and repeated in some cases. The study indicated that lesions can be prevented by applying a light splint in the early stages of deformity and that when dietary planning with balanced commercial foods is enforced deformity does not recur in later stages.
The observation was carried out in a group of 70 female cattle in the course of two years. The animals were kept in suckler herds. They were provided with pasture grass and maize silage indoors during the grazing season and with forage ration (silage and hay) in winter. The aim of the study was to assess the effect of year (2001 and 2002), season (spring and autumn), breed (Aberdeen Angus AA, Beef Simmental BS, Blonde d'Aquitaine BA, Charolais CH, Hereford HE and Limousin LI) and reproduction cycle (pregnant heifers PH, non-pregnant heifers NPH, pregnant cows PC, non-pregnant cows NC and lactating cows with calves LCC) on some blood plasma parameters. Mean values of these parameters were following: Total protein 72.37 gl⁻¹ , glucose (Glu) 3.23 mmol∙l⁻¹, urea (Urea) 4.39 mmol∙l⁻¹, bilirubin (Bilir) 4.63 mmol∙l⁻¹, alkaline phosphatase (ALP) 1.02 µkat∙l⁻¹, asparate aminotransferase (AST) 1.29 µkat∙l⁻¹, gamma glutamyltransferase (GMT) 0.30 µkat∙l⁻¹, sodium (Na) 114.33 mmol∙l⁻¹, calcium (Ca) 2.30 mmol∙l⁻¹ and phosphorus (P) 1.99 mmolT1. Year affected TP, Glu, Bilir, ALP, AST (all p<0.01) and urea (p<0.05). Season affected TP, Glu, urea, Bilir, Ca, P (all p<0.01) . There were significant differences in urea, GMT, Ca and P between breeds. Reproduction cycle affected TP and Glu (p<0.05). It can be concluded that year and season affected the observed blood plasma parameters considerably more than breed or reproduction cycle.
The aim of the study was to evaluate the variability of morphological characteristics of native middleEuropean bees (Apis m. mellifera) of the ‘Northern M’ line. The research covered characteristics of breed (the length of proboscis, the cubital index), body size (the width of tergite 4 and the sum of widths of tergites 3 and 4) and wing size (length and width). The study compared bees harvested from a leading apiary and from collaborating apiaries participating in a program for the protection of genetic resources of bees of this line. The material for the study was harvested in 10 consecutive years. The samples were collected by the “cluster drawing” method (the multi-stage method of clustering described by Zee et al. in 2013). Each sample consisted of 25 to 30 bees. The frames were loaded in an instrument for the morphological measurement of bees (Apimeter). Seven measurements were taken on prepared body parts of each bee. The length and width of the wing and the length of the cubital vein were measured on the right front wing (hereinafter referred to as the “wing”). In addition, the width of abdominal tergites 3 and 4 and the length of proboscis were measured in each instance. In total, 4291 bees were harvested and 30 037 measurements were taken. The conclusion is that the program for the protection of genetic resources of bees of the ‘Northern M’ line can be implemented in Poland based on the leading apiary and on the collaborating apiaries, and bees of this line display characteristics of middleEuropean bees. Moreover, the study demonstrated a consistency of values of the studied characteristics of the ‘Northern M’ line with the applicable references of morphological characteristics for Apis m. mellifera. In addition, based on a review of results of the author’s research and based on collected literature originating from the 1960s, the study proves that a dwarfing trend has emerged among middle-European bees.
The principal objective of this study was to evaluate the processing value of pork obtained from different breeds reared in Poland. The research material consisted of a part of the m. longissimus thoracis obtained from pigs of different breeds: Duroc, Pietrain, Polish Large White, Polish Landrace and Line 990. It was found that the breed is responsible for significant differences in the quality features of pork. Among the samples analysed, meat from Duroc porkers proved to be of the highest, while that from Pietrain porkers of the lowest quality. Pork from the Duroc breed had the highest content of intramuscular fat, which affected the shear and compression force, two traits having a significant effect on sensory quality. Pork from Pietrain porkers was estimated to have a low processing as well as culinary quality. The significantly lower pH, observed in pork from this breed, resulted in a higher colour lightness and lower water holding capacity. Additionally, such pork demonstrated a hard texture (high shear and compression force). On the basis of the study results one may state that programmes aiming at the improvement of pork quality in Poland should include a wider use of the Duroc and limit the use of the Pietrain breed.
The aim of the study was comparison of the milk performance of primiparous cows from 6 European countries with Polish Holstein-Friesians, as well as to demonstrate the differences within the different genetic groups. Material consisted of data contained in the dairy reports gathered from 26 farm “Healthy Cow” project. The analysis involved data including milk yield and its chemical composition, i.e.: fat, protein, lactose, solids, and urea somatic cells count in milk. On the basis of these results, it was found that the country of origin had significant impact on daily milk yield, % fat, % protein, % of lactose and also affected dry matter content, urea and somatic cells in the milk of lactating cows. Research reviled that there were not differences in milk performance between imported and native cows. However, the breed of cows had an strong impact on daily milk production, % protein, % lactose,% dry weight, as well as urea content and somatic cells count.
LHX4 is a LIM homeodomain transcription factor involved in pituitary ontogenesis. Some mutations of LHX4 are associated with combined pituitary hormone deficiency that affects growth and development in animal. The objective of this study is revealing to these polymorphisms of in LHX4 gene exons 1, 2, 3 in 820 Chinese cattle and to assess possible association of polymorphisms in LHX4 gene with growth traits in NY breed. The PCR-SSCP and DNA sequencing of LHX4 gene showed five novel synonymous mutations: (GenBank: NW_001493442.2: g.35143G >A, g.35152C>T, g.35212C>T, g.35230G>A, g.35233T>C). Genotype MM (GG-CC-CC-GG-TT) and haplotype M (G-C-C-G-T) were dominant in the four breeds, and genotype frequencies of LHX4 in the four cattle populations agreed with Hardy-Weinberg equilibrium (P>0.05), although their frequencies significantly differed among the four analyzed populations (***P<0.001). The association analysis showed that individuals with genotype MM had greater body weight than those with genotype MN (*P<0.05) at eighteen months of age in Nanyang cattle.
A simple cryopreservation method described in 1995 by Tselutin et al. was used for freezing the semen of four fancy fowl breeds: White Crested Black Polish (WCBP), Greenleg Partridge (GP),Italian Partridge (IP) and Black Minorca (BP). The differences in quality (ejaculate volume,osmotic pressure, sperm concentration and morphology) of fresh semen between evaluated breeds were observed, as well as the differences in semen freezability. The freezing-thawing process caused significant (P≤0.01) decrease in percentage of live, normal spermatozoa, with coincident increase in percentage of dead spermatozoa and spermatozoa with acrosome defect. In relation to the fresh semen, the number of live, normal spermatozoa that survived cryopreservation procedure constituted 18.1% in WCBP, 25.1% in GP, 26.2% in IP and 33.6% in BM semen.
Analysis of the inbreeding level in the active population of pigs of different breeds in Poland. Pig breeders in Poland have made extensive use of the BLUP-AM method since 1999. The application of this method for evaluating the breeding value of pigs improves their breeding value in nucleus herds and in the general population. On the other hand, the use of many sources of information (including pedigree information) in the calculations considerably increases the risk of inbreeding. The objective of the study* was to estimate the level of inbreeding in sows and boars from the domestic pig population, which have been selected for more than ten years based on the BLUP-AM estimated breeding value. The study involved Polish Large White, Polish Landrace, Hampshire, Duroc, Pietrain and Puławska sows and boars born between 2007 and 2011. Data on a total of 16 842 sows and 977 boars representing the 6 breeds raised in Poland were collected for the pedigree analyses. During the analyzed period, the coefficient of inbreeding in sows ranged from F = 0 to F = 0.25 (14 animals). Most of the animals were non-inbred (70% in the PLW and Puławska, over 80% in the PL, Duroc and Pietrain and 100% in the Hampshire). For the boars, the coefficient of inbreeding varied from 0 to 0.1875 (1 animal). The proportion of non-inbred boars was 77% in the PLW, 91% in the PL, 63% in the Puławska, and about 80% in the Duroc and Pietrain breeds. The results point to the appropriate structure of the pig population in terms of inbreeding, which shows that the selection of animals for mating has been correct and there have been no negative consequences of using the BLUP method.
The study was conducted to find out the most reliable parameter of the hoof size in relation to the horse body size, exemplified in mares. The mares of four breeds belonging to different origin types were examined: Purebred Arabian, halfbred Anglo-Arabian, primitive Polish Konik and Polish Cold-Blood, 77 mares in total. The mares were four to 13 years old, classified into three age groups. Three body measurements were taken: height at withers, chest circumference and canzon circumference. The boniness index (cannon circumference to height at withers ratio) was also defined. After trimming, three left fore hoof measurements were taken: toe length, solar length and hoof width. Total length and width were calculated as a hoof solar size measure. On the basis of the parameters obtained, nine fore hoof to body dimension ratios were defined. To evaluate the results, least squares means analysis was used and correlation coefficients between body parameters (1), between hoof parameters (2), as well as between body and hoof parameters (3) were identified. The results show the hoof to body dimension ratios grow according to the increasing canzon circumference to height at withers ratio. The hoof width to chest circumference ratio was found to be a useful parameter of the hoof size. The means (%) obtained (5.93±0.10, 6.41±0.08, 6.56±0.11 and 7.26±0.09 in Purebred Arabian, Anglo-Arabian, Polish Konik and Polish Cold-Blood horses,respectively) are suggested as standards to which individual ratios in mares of similar breeds May be compared judging the horse’s conformation. The age hardly affected the hoof solar size to height at withers ratio in mares four to nine years old.
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