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A comparative study of the oil yield and fatty acid composition of three Salvia species seeds collected in different locations has been conducted. Seed oil extraction was made using a Soxhlet-extractor and fatty acid analysis was undertaken using a GC-FID. The effect of the collecting site on oil yield, as well as the content of individual fatty acid and total fatty acid and fatty acid content was significant. Seed oil yield varied from 14.94 to 22.83% and the total fatty acids ranged from 67.36 to 82.49 mg/g DW. α-Linolenic (24.02-49.19%), linoleic (20.13-42.88%), oleic (12.97-17.81%) and palmitic (8.37-16.63%) acids were the most abundant fatty acids in all analyzed samples. α-Linolenic acid was found to be the major fatty acid in S. verbenaca and S. officinalis species, however, S. aegyptiaca was characterized by the prevalence of linoleic acid. Among the unsaturated fatty acids, which were represented in all samples in high amounts (78.16-89.34%), the polyunsaturated fatty acids (α-linolenic and linoleic acids) showed important levels ranging from 63.09 to 74.71%. Seeds of S. verbenaca were the richest in polyunsaturated fatty acids.
Salinity stress is a serious problem in urban landscape of arid and semi arid regions. To overcome the adverse impact of salinity, the application of organic matter and plant nutrients in the growth media for improving the plant growth is essential. An experiment was conducted in order to determine the response of Salvia splendens to salinity levels and also the role of humic acid in the salt stress alleviation. In the current experiment, five salinity levels (0, 20, 40, 60, and 80 mM NaCl) and three humic acid (0, 100, 500 and 1000 mg/l) treatments were prepared. The effects of these treatments were investigated on some growth parameters, physiological characteristics and also biochemical compounds. The results indicated that the growth parameters decreased in saline-treated than control plants. Different salinity levels significantly affected relative water content, evaporation rate and also electrolyte leakage. Salinity caused the increase in proline, malondialdehyde, sugar content, DPPH, total phenol content and decrease in chlorophyll, compare to the control plants. Application of humic acid on Salvia splendens decreased the leaf area and plant height compared to the control plants. Thus, regarding the growth parameters, it is probable that the effect of humic acid on the biochemical compounds is similar to salinity effect. The findings suggest that sage is an ornamental plant sensitive to salinity and humic acid (in the studied levels) could not alleviate the negative effects of salt stress on this plant.
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