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Comparison of phenylethanoids content in Rhodiola kirilowii and Rhodiola rosea roots applying a newly developed UPLC-MS/MS method

100%

Gryszczynska A., Lowicki Z., Opala B., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Mrozikiewicz P. M.

Herba Polonica

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2012

|

tom 58

|

nr 4

A concentration of two phenylethanoids in the roots of two species: Rhodiola kirilowii and rosea were compared, aqueous and hydroalcoholic extracts from those plants were also analyzed. In order to determine the content of p-tyrosol and salidroside, the ultra performance liquid chromatography connected with a tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The obtained results shown that content of measured phenylethanoids depends on Rhodiola species. Roots of R. kirilowii contain more p-tyrosol, while R. rosea roots are reacher in salidroside. Our results indicate that the application of UPLC MS/MS method allows to determine the phenylethanoids content in tested samples with satisfactory precision.

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Zielarz [nie]znany - rozeniec

100%

Papis P.

Ogrodnik Polski. Magazyn dla Gospodarstw Ogrodniczych

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2005

|

nr 02

77

3

Rhodiola rosea L. [rozeniec gorski] - stan badan biotechnologicznych

86%

Krajewska-Patan A., Dreger M., Gorska-Paukszta M., Lowicka A., Furmanowa M., Mrozikiewicz P. M.

Herba Polonica

|

2005

|

tom 51

|

nr 3-4

50-63

Rhodiola rosea L. (różeniec górski) to bylina należąca do rodziny Crassulaceae, występująca na terenach północnych Azji, górskich regionach Europy oraz w subarktycznych rejonach Ameryki Północnej. Różeniec górski od wieków jest stosowany w tradycyjnej medycynie azjatyckiej. Przypisuje mu się działanie stymulujące ośrodkowy system nerwowy, działanie zwiększające fizyczną i umysłową wydolność organizmu, zmniejszające zmęczenie, a także działanie adapto- genne, kardiochronne, przeciwnowotworowe i przeciwutleniające. Istnieje niewiele badań farmakologicznych czy klinicznych potwierdzających tak szeroki zakres działania wyciągów z Rhodiola rosea. Najbardziej interesującym aspektem działania tej rośliny są jej własności psychostymulujące, które wzbudzają duże nadzieje na wykorzystanie w medycynie lub w dodatkach funkcjonalnych diety. Korzenie różeńca górskiego zawierają bardzo wiele związków biologicznie czynnych, takich jak fenylopropanoidy (rozawiny), związki fenolowe (salidrozyd), monoterpeny, flawonoidy, p-tyrozol i kwasy organiczne. Wyciągi z różeńca mają rozległy zakres działania fizjologicznego, włącznie ze zmianą poziomu neurotransmiterów, aktywnośa centralnego układu nerwowego i układu krążenia. Psychostymulujące działanie kojarzone jest ze zmianami poziomu dopaminy i serotoniny w mózgu. Własności adaptogenne wiąże się ze zdolnością wpływania na poziom i aktywność niektórych monoamin i peptydów opioidowych, takich jak beta-endorfiny. Aktywność przeciwutleniająca i działanie ochronne na różne tkanki organizmu wyciągów z Rhodiola rosea potwierdzono w licznych pracach naukowych. Biotechnologiczne badania nad różeńcem górskim są prowadzone w wielu krajach (m.in. w Rosji, Finlandii, Niemczech i Polsce). Celem naukowców jest zwiększenie zawartości związków biologicznie czynnych w kulturach tkankowych za pomocą elicytacji czy biotrans- formacji, co jest spowodowane wzrastającym obecnie zainteresowaniem przemysłu farmaceutycznego, kosmetycznego i spożywczego nowymi, alternatywnymi sposobami otrzymywania cennego surowca roślinnego.

4

Phytochemical and pharmacological properties of Rhodiola rosea L.

86%

Furmanowa M., Kedzia B., Hartwich M., Kozlowski J., Krajewska-Patan A., Mscisz A., Jankowiak J.

Herba Polonica

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1999

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tom 45

|

nr 2

108-113

Badania obejmowały wyciągi etanolowe z korzenia, hodowli tkankowych i tkanki kalusowej Rhodiola rosea L. Stwierdzono, że największą zawartość o-dihydroksyfenoli, a także najsilniejsze działanie przeciwutleniające i adaptogenne wykazywał wyciąg z korzenia Rhodiola rosea. Jego działanie adaptogenne porównywalne było z analogicznymi wyciągami z korzenia Panax ginseng i ziela Echinacea purpurea. Wyciągi z hodowli zawiesinowych i tkanki kalusowej odznaczały się wielokrotnie mniejszą zawartością o-dihydroksyfenoli oraz zdecydowanie słabszym działaniem przeciwutleniającym i przeciwstresowym. Natomiast ich działanie immunostymulujące i zwiększające siłę mięśniową zwierząt doświadczalnych kształtowało się na poziomie wyciągu z korzenia Rhodiola rosea.

5

Rozeniec gorski Rhodiola rosea L.

86%

Hartwich M.

Wiadomości Zielarskie

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1997

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tom 39

|

nr 10

9-10

6

Transgenic callus culture establishment, a tool for metabolic engineering of Rhodiola rosea L.

86%

Mirmazloum I., Forgacs I., Zok A., Pedryc A., Gyorgy Z.

Acta Scientiarum Polonorum. Hortorum Cultus

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2014

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tom 13

|

nr 4

Agrobacterium tumefaciens EHA101 (pTd33) strain carrying uidA (GUS) reporter gene was used in model experiments on roseroot callus transformation. The T-DNA of pTd33 binary vector plasmid harbors nptII gene conferring resistance to kanamycin, and a uidA reporter gene, encodes the ß-glucuronidase enzyme. Roseroot seeds were sterilized and germinated on half strength MS media of which 70% germinated without any pretreatment. Calli were obtained from leaf segments of the in vitro grown seedlings. Calli was grown on solid MS medium supplemented with 1 mg l⁻¹ NAA and 0.5 mg l⁻¹ BAP. Different types of calli were obtained of which the green and compact type was chosen for transformation experiments. After co-cultivation with agrobacteria, calli were transferred to the same medium supplemented with 20 mg l⁻¹ kanamycin, 200 mg l⁻¹ carbenicillin and 300 mg l⁻¹ claforan with antioxidants (Polyclar and DTE) for selection. GUS test using a titron buffer was applied for monitoring the transformation of the calli. DNAs of 20 individual samples was extracted and subjected for PCR analysis proved the stable transformation in all of the taken samples by amplifying the nptII gene fragment. The method introduced here can be a tool for inserting and over-expressing the genes encoding for hypothesized enzymes to be involved in the biosynthesis of pharmaceutically important bioactive molecules of roseroot and therefore facilitating the applications for callus culture of roseroot in different bioreactor systems for pharmaceutical productions.

7

Influence of salidroside, a neuroactive compound of Rhodiola rosea L., on alcohol tolerance development in rats

86%

Szulc M., Mularczyk P., Kujawski R., Gryszczynska A., Kaminska E., Geppert B., Baraniak J., Kania- Dobrowolska M., Ozarowski M., Krajewska-Patan A., Mikolajczak P. L.

Herba Polonica

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2018

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tom 64

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nr 1

In recent years, the search for potential neuroprotective properties of salidroside and its ability to influence the activity of nervous system become the subject of intense studies of many research groups. None of these studies, however, include an attempt to determine the effect of salidroside on the course of alcohol tolerance in vivo. The aim of this study was to investigate the ability of salidroside to inhibit the development of alcohol tolerance in rats, determining whether the effect of its action may occur in a dose-dependent manner, reducing both metabolic and central tolerance without affecting body temperature in control rats. Male Wistar rats were injected daily with ethanol at a dose of 3 g/kg for 9 consecutive days to produce ethanol tolerance. Salidroside in two doses (4.5 mg/kg and 45 mg/kg b.w.) or vehiculum was administered orally. On the 1st, 3rd, 5th and 8th day a hypothermic effect of ethanol was measured, while the loss of righting reflex procedure was performed on the 2nd, 4th, 6th and 7th day. On the 9th day rats were treated with salidroside, sacrificed 1 h after ethanol injections and blood was collected for blood-ethanol concentration measurement. Salidroside at a dose of 45 mg/kg inhibited the development of tolerance to hypothermic and sedative effects of ethanol, whereas insignificant elevation of blood-ethanol concentration was observed. The dose of 4.5 mg/kg b.w. had minimal effect, only small inhibition of tolerance to hypothermic action was observed. Salidroside affected neither body mass growth nor body temperature in non-alcoholic (control) rats.Results of the study indicate that salidroside at a dose of 45 mg/kg inhibited the development of tolerance to the hypothermic effect of ethanol. Observed inhibition of tolerance to the sedative effect of ethanol seems to be associated with salidroside influence on the central nervous system. A comprehensive explanation of the abovementioned observations requires further pharmacological and pharmacodynamic studies.

8

Determination of lotaustralin in Rhodiola species

86%

Gryszczynska A., Lowicki Z., Opala B., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Mrozikiewicz P. M.

Herba Polonica

|

2013

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tom 59

|

nr 2

In our research, the concentration of lotaustralin in the roots of two species Rhodiola kirilowii and Rhodiola rosea were compared. Aqueous and hydroalcoholic extracts from those plants were analyzed too. To determine the content of this compound the ultra performance liquid chromatography – tandem mass spectrometry (UPLC-MS/MS, Waters) was used. The obtained results showed that the content of measured lotaustralin depends on the species of Rhodiola. R. rosea roots are the richer source of lotaustralin then R. kirilowii. The same situation was observed in the extracts. A hydroalcoholic extract from R. rosea contains up to 135.276 mg of lotaustralin in 100 g of dry powdered material. In the case of R. kirilowii extracts, an aqueous extract contained more lotaustralin (74.791 mg/100 g of dry powdered material) then a hydroalcoholic extract.

9

The influence of elicitation on the tissue cultures of roseroot [Rhodiola rosea L.]

86%

Krajewska-Patan A., Mscisz A., Kedzia B., Lutomski J.

Herba Polonica

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2002

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tom 48

|

nr 2

In this study the influence of the elicitation with yeast extract on the content of salidroside in the callus tissue of Rhodiola rosea was investigated. The colorimetric method for salidroside determination was used for quantitative analysis of this compound. Two series of experiments showed that the elicitor increased the salidroside content from 0.8% to 1.4% and from 0.8% to 1.5% respectively. The salidroside content in the elicited callus was twice as high as in five-year old roots of intact plants. Two types of pharmacological studies were used for the estimation of the adaptogenic activity of R. rosea extracts: determination of immunostimulating activity and determination of antistress effect.

10

Glucosylation of p-tyrosol to salidroside by Rhodiola rosea L. cell cultures

86%

Furmanowa M., Hartwich M., Alfermann A. W.

Herba Polonica

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2002

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tom 48

|

nr 2

Na drodze biotransformacji (glukozylacji) w hodowlach komórkowych Rhodiola rosea z p-tyrozolu wytwarzano salidrozyd. Substrat był przetwarzany w 57% po 72 godz. inkubacji. Obecność salidrozydu i p-tyrozolu stwierdzano przy użyciu TLC i RP-HPLC w przestrzeniach międzykomórkowych wszystkich komórek R. rosea. Zawartość salidrozydu kształtowała się w granicach 1,21-2,31% suchej masy komórek.

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Cytological changes in HL-60 cells after treatment with an extract from Rhodiola rosea rhizomes

86%

Majewska A., Hoser G., Furmanowa M., Urbanska N., Kuras M.

Cellular and Molecular Biology Letters

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2002

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tom 07

|

nr Suppl.

12

Rozmieszczenie i biologia rozenca gorskiego Rhodiola rosea L. w polskich Karkonoszach

72%

Krukowski M., Krakowski K., Malicki M., Szczesniak E.

Przyroda Sudetów

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2009

|

nr 12

3-8

13

Effect of fertilization on roseroot (Rhodiola rosea L.) yield and content of active compounds

72%

Buchwald W., Mordalski R., Kucharski W. A., Gryszczynska A., Adamczak A.

Acta Scientiarum Polonorum. Hortorum Cultus

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2015

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tom 14

|

nr 2

Roseroot (Rhodiola rosea L.) is an important medicinal plant with welldocumented adaptogenic activity. The decrease in its natural resources induces to undertake research on the introduction of this species into cultivation. The aim of the present work was to determine the effect of organic and mineral fertilization on increasing the biomass of underground parts of Rh. rosea and the level of biologically active compounds. Throughout the study period, the highest raw material yield was obtained after the application of the following doses of mineral fertilization: N – 60.0 kg·ha-1, P – 35.2 kg·ha-1, K – 83.0 kg·ha-1, without manure. The use of manure caused a decrease in the average rhizome and root weight by more than 20%. Mineral fertilization application increased the average raw material yield by about 30–40% and allowed obtaining the highest weight of underground organs of the species in question already in the fourth year of cultivation. Furthermore, the present study demonstrates that mineral fertilization does not affect substantially the level of the individual groups of compounds in the raw material, but the use of manure may significantly reduce the content of phenylpropanoids. The level of active compounds is also influenced by crop age. Two-year-old plants were characterized by the highest content of phenylpropanoids as well as by the lowest content of phenylethanoids and phenolic acids. In the following years of cultivation, no statistically significant changes were observed in the level of the analysed groups of compounds.

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Dose-dependent in vivo effect of Rhodiola and Echinacea on the mitogen-induced lymphocyte proliferation in mice

72%

Skopińska-Rozewska E., Sokolnicka I., Siwicki A. K., Stankiewicz W., Dabrowski M. P., Buchwald W., Krajewska-Patan A., Mielcarek S., Mscisz A., Furmanowa M.

Polish Journal of Veterinary Sciences

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2011

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tom 14

|

nr 2

Echinacea purpurea (EP) and Echinacea angustifolia (EA) are ones of the most important world’s herbs with immunotropic activity. They were traditional medicinal plants used by North American Indians for the treatment of various illnesses. Now they are cultivated in many countries and are used mainly to treat respiratory tract infections. Rhodiola rosea (RR) and Rhodiola quadrifida (RQ) are medicinal plants originated from Asia and used traditionally as adaptogens, antidepressants, and anti-inflammatory remedies. We previously reported, that extracts of underground parts of RR and RQ exhibited immunotropic activity. We have demonstrated in pigs that in vitro RR or RQ supplementation of blood lymphocyte cultures stimulated T cell proliferative response to Con A in lower, and inhibited it in higher Rhodiola extract concentrations. The aim of this work was to evaluate the in vivo effect of these herbal remedies on the in vitro proliferative response of mouse splenic lymphocytes to another T-cell mitogen- Phaseolus vulgaris haemagglutinin (PHA). We have found significant stimulation of proliferative response, in comparison to the controls, in mice fed lower doses of tested remedies, and inhibition, no effect or lower stimulation, in mice fed higher doses of these drugs.

15

Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

72%

Gryszczynska A., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Rudzinska K., Mroziewicz P. M.

Herba Polonica

|

2012

|

tom 58

|

nr 3

The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.

16

Rozaniec gorski

72%

Ciecinski J.

Aura

|

1995

|

nr 01

24-25

A Polish representative of the Rhodiola genus (Rhodiola rosea) is an arctic-alpine species occurring in Eurasia and North America, in polar tundra and high mountains, from the Pyrenees to the Alps, Carpathian Mountains, Siberia and Kamchatka to Alaska. In Poland this species occurs in the Sudety, Tatra and Bieszczady Mountains, in national parks only. Among plants with ginseng-like effects, this species is the strongest. Its preparations increase the ability to do physical and mental work, regulate blood pressure and improve reflexes, visual and aural acuity. They also have atimulative and strengthening effects.

17

Biologia kielkowania nasion roslin leczniczych. Cz.22. Gatunki z rodziny gruboszowatych [Crassulaceae]. Rhodiola rosea L.

72%

Kozlowski J., Szczyglewska D.

Herba Polonica

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2001

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tom 47

|

nr 2

137-141

Opisano cechy morfologiczne nasion i siewek Rhodiola rosea L. Ustalono optymalne warunki kiełkowania nasion w laboratorium. Prześledzono także zmiany zdolności kiełkowania nasion w pierwszym roku po zbiorze dokonując comiesięcznych analiz. Stwierdzono, że nasiona Rhodiola rosea L. do kiełkowania wymagają dostępu światła. Zdolność kiełkowania należy ustalać po 21 dniach od rozpoczęcia analizy. Nasiona Rhodiola rosea L. w ciągu pierwszego roku po zbiorze kiełkują w liczbie od 40 do 60 %. Przechowywane w nie ogrzewanym magazynie zachowują żywotność nawet do 10 lat.

18

Proanthocyanidins in Rhodiola kirilowii and Rhodiola rosea callus tissues and transformed roots - determination with UPLC-MS/MS method

72%

Gryszczynska A., Krajewska-Patan A., Dreger M., Buchwald W., Pietrosiuk A., Zych M., Karasiewicz M., Bogacz A., Kujawski R., Furmanowa M., Czerny B., Mielcarek S., Gazda P., Mrozikiewicz P. M.

Herba Polonica

|

2012

|

tom 58

|

nr 4

Several species of Rhodiola genus (Crassulaceae family) like Rhodiola kirilowii and Rhodiola rosea are used in official or traditional medicine. The aim of this study was to determine qualitative and quantitative content of proanthocyanidins using ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) in the callus tissues and in the transformed roots (infected by Agrobacterium rhizogenes LBA 9402 strain) of R. kirilowii and R. rosea. This validated assay allows to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG). Our results concerning the material from in vitro cultivation of R. kirilowii and R. rosea indicate that R. rosea callus can be a better source of catechin when compared with other tested materials, especially when the content of (-)-gallate epigallocatechin is taken under consideration (3.429 mg/100 g of dry powdered material). The application of UPLC MS/MS method allowed to determine the content of proanthocyanidins in tested samples with satisfactory precision and can be used in the phytochemical investigations of Rhodiola sp. in vitro cultivated tissues.

19

Determination of chlorogenic and gallic acids by UPLC-MS/MS

72%

Gryszczynska A., Opala B., Lowicki Z., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Boron D., Bogacz A., Mrozikiewicz P. M.

Herba Polonica

|

2013

|

tom 59

|

nr 1

The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.

20

Rhodiola rosea L. [rozeniec gorski] w kulturach in vitro

72%

Dreger M., Krajewska-Patan A., Furmanowa M., Mscisz A., Lowicka A., Gorska-Paukszta M., Mrozikiewicz P. M.

Herba Polonica

|

2007

|

tom 53

|

nr 2

35-36

Ograniczanie wyników

Comparison of phenylethanoids content in Rhodiola kirilowii and Rhodiola rosea roots applying a newly developed UPLC-MS/MS method

Zielarz [nie]znany - rozeniec

Rhodiola rosea L. [rozeniec gorski] - stan badan biotechnologicznych

Phytochemical and pharmacological properties of Rhodiola rosea L.

Rozeniec gorski Rhodiola rosea L.

Transgenic callus culture establishment, a tool for metabolic engineering of Rhodiola rosea L.

Influence of salidroside, a neuroactive compound of Rhodiola rosea L., on alcohol tolerance development in rats

Determination of lotaustralin in Rhodiola species

The influence of elicitation on the tissue cultures of roseroot [Rhodiola rosea L.]

Glucosylation of p-tyrosol to salidroside by Rhodiola rosea L. cell cultures

Cytological changes in HL-60 cells after treatment with an extract from Rhodiola rosea rhizomes

Rozmieszczenie i biologia rozenca gorskiego Rhodiola rosea L. w polskich Karkonoszach

Effect of fertilization on roseroot (Rhodiola rosea L.) yield and content of active compounds

Dose-dependent in vivo effect of Rhodiola and Echinacea on the mitogen-induced lymphocyte proliferation in mice

Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

Rozaniec gorski

Biologia kielkowania nasion roslin leczniczych. Cz.22. Gatunki z rodziny gruboszowatych [Crassulaceae]. Rhodiola rosea L.

Proanthocyanidins in Rhodiola kirilowii and Rhodiola rosea callus tissues and transformed roots - determination with UPLC-MS/MS method