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From 2008 to 2010 the levels of sugar beet seedlings infection caused by Rhizoctonia solani were compared in laboratory tests. Seven sugar beet lines were tested: H56, H66, S2, S3, S4, S5 and S6 as well as three control cultivars: Carlos, Esperanza and Janosik. Sugar beet lines with tolerance to rhizoctoniosis and cultivars without tolerance were infected artificially by R. solani isolates: R1, R28a and R28b. These isolates belong to the second anastomosis group (AG), which is usually highly pathogenic to beet roots. The aim of the experiment was to test whether the tolerance of sugar beet genotypes to R. solani AG 2 prevents both root rot, and damping-off of seedlings, induced by the pathogen. Sugar beet lines tolerant to brown root rot in laboratory tests were significantly less sensitive to infection of the seedlings by R. solani AG 2 isolates in comparison to control cultivars. Rhizoctonia solani AG 2 isolates demonstrated considerable differences in pathogenicity against seedlings of sugar beet lines and cultivars. The strongest infection of sugar beet seedlings occurred with the isolate R28b. The greatest tolerance to infection by AG 2 isolates was found for the S5 and S3 breeding lines.
Siderophore production is an important mechanism of biological control by a number of strains of plant growth-promoting rhizobacteria. Pseudomonas fluorescens UTPF5 was originally obtained from onion field. Biochemical and physiological characteristics of this strain refer to biovar 3 of P. fluorescens. Strain UTPF5 is an effective bacterium against several phytopathogenic fungi. Pyoverdine type siderophore of this strain was isolated using XAD amberlite column. The plant growth promotion and antifungal properties of bacteria were demonstrated under greenhouse conditions in combination with Fe-EDTA, Fe-EDDHA and Zn as modulators of pyoverdine production. Amendment with zinc, Fe-EDTA and Fe-EDDHA suppressed the disease inhibition when partially used with UTPF5. 7NSK2 and its pyoverdine mutant, MPFM1, were used as reference strains the inhibition percent of which was not affect by soil amendment. Iron chelates, especially Fe-EDDHA, increased growth and chlorophyll production by plants. This effect was improved in the presence of bacterial strains. The siderophore mutant MPFM1 did not exhibit satisfactory disease inhibition and growth promotion activity. In vitro experiments showed that purified pyoverdine could decrease the fungal growth to the same extent as pyoverdine-producing strain.
In this study, the biocontrol abilities of water-soluble and volatile metabolites of three different isolates of Trichoderma (T. asperellum,T. harzianum and Trichoderma spp.) against soil borne plant pathogen Rhizoctonia solani were investigated both in vitro and in vivo. The results showed for the first time that mycelial growth inhibition of the pathogen was 74.4–67.8% with water-soluble metabolites as compared to 15.3–10.6% with volatile metabolites in vitro. In vivo antagonistic activity of Trichoderma isolates against R. solani was evaluated on bean plants under laboratory and greenhouse conditions. We observed that T. asperellum was more effective and consistent, lowering disease incidence up to 19.3% in laboratory and 30.5% in green house conditions. These results showed that three isolates of Trichoderma could be used as effective biocontrol agents against R. solani.
A shift towards organic farming suggests amalgamation of organic resources against soil borne plant pathogens. The influence of metabolites of most ubiquitous Aspergillus spp., organic amendment extracts and their combined effect with Trichoderma virens were evaluated in vitro against Rhizoctonia solani. The minimum (36.1 mm) growth was attained by R. solani in co-culture with A. niger. The maximum (42.3 mm) inhibition of mycelial growth of the test organism was observed with culture filtrate of A. ochraceous followed by A. niger, A. fumigatus, A. flavus and A. terreus. Among organic amendment extractants, castor cake exhibited an additive effect on the growth of T. virens, however, the maximum (41.8 mm) suppressive effect on R. solani was observed with vermicompost. With the advance in time, the effect of organic amendment extracts increased markedly. Inhibition potential of culture filtrate mixturte of A. niger + T. virens and A. ochraceous + T. virens against R. solani was significantly higher in comparison to the other combinations.
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