Wyniki wyszukiwania

1

Expression of estrogen, estrogen related and androgen receptors in adrenal cortex of intact adult male and female rats

100%

Trejter M., Jopek K., Celichowski P., Tyczewska M., Malendowicz L. K., Rucinski M.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 2

2

How the RNA isolation method can affect microRNA microarray results

100%

Podolska A., Kaczkowski B., Litman T., Fredholm M., Cirera S.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

The quality of RNA is crucial in gene expression experiments. RNA degradation interferes in the measurement of gene expression, and in this context, microRNA quantification can lead to an incorrect estimation. In the present study, two different RNA isolation methods were used to perform microRNA microarray analysis on porcine brain tissue. One method is a phenol-guanidine isothiocyanate-based procedure that permits isolation of total RNA. The second method, miRVana™ microRNA isolation, is column based and recovers the small RNA fraction alone. We found that microarray analyses give different results that depend on the RNA fraction used, in particular because some microRNAs appear very sensitive to the RNA isolation method. We conclude that precautions need to be taken when comparing microarray studies based on RNA isolated with different methods.

3

Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

84%

Ferry A. E., Pace B. S.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 4

Obtaining adequate samples for nucleic acid or protein analysis from a limited number of cells can be a difficult task. The steps for isolation of DNA, cytoplasmic RNA and nuclear proteins from mononuclear cells collected from a single peripheral blood sample are outlined below. A previously described technique for rapid isolation of nuclear proteins was modified to acquire RNA and DNA of sufficient quantity and quality to perform analyses at the molecular level without altering the quality of protein extracted. This approach is applicable for use with peripheral blood mononuclear cells, primary cultures and immortalized cell lines.

4

Detection of Strawberry mottle virus [SMoV] using RT-PCR - comparison of two RNA extraction methods

84%

Cieslinska M.

Journal of Fruit and Ornamental Plant Research

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2004

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tom 12

5

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Optimization of activated sludge storage before RNA isolation

67%

Cydzik-Kwiatkowska A., Wnuk M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

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nr 1

6

Macrophage phenotype in the ocular surface of experimental murine dry eye disease

67%

You I.-C., Coursey T. G., Bian F., Barbosa F. L., de Paiva C. S., Pflugfelder S. C.

Archivum Immunologiae et Therapiae Experimentalis

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2015

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tom 63

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nr 4

7

Molecular studies in osteogenesis imperfecta [OI] III. cDNA of COL1A1 and COL1A2 analysis using the BESS-T-Scan technique

67%

Sucharski P., Sanak M., Kostyk E., Pietrzyk J. J., Kruczek A.

Journal of Applied Genetics

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1998

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tom 39

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nr 4

A BESS-T-Scan analysis of cDNA COL1A1 and COL1A2 obtained by RT-PCR derived from five patients with sporadic forms of ostegenesis imperfecta was performed. The study was done in four patients with type I and one patient with type III OI. The analysis revealed the presence of structural changes in two regions of cDNA COL1A1 in two patients. No quantitative changes referring to COL1A2 gene were noted in any patient. The above analysis was the first application of the BESS-T-Scan technique in a molecular diagnosis of OI. The applied method seems to be useful and fulfil the basic criteria of the screening method to detect and locate mutations.

8

An improved method for RNA isolation from plants using commercial extraction kits

59%

Kalinowska E., Chodorska M., Paduch-Cichal E., Mroczkowska K.

Acta Biochimica Polonica

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2012

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tom 59

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nr 3

Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.

Ograniczanie wyników

Expression of estrogen, estrogen related and androgen receptors in adrenal cortex of intact adult male and female rats

How the RNA isolation method can affect microRNA microarray results

Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

Detection of Strawberry mottle virus [SMoV] using RT-PCR - comparison of two RNA extraction methods

Optimization of activated sludge storage before RNA isolation

Macrophage phenotype in the ocular surface of experimental murine dry eye disease

Molecular studies in osteogenesis imperfecta [OI] III. cDNA of COL1A1 and COL1A2 analysis using the BESS-T-Scan technique

An improved method for RNA isolation from plants using commercial extraction kits