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Screening of cellulase and pectinase by using Pseudomonas fluorescens and Bacillus subtilis

100%

Reetha S., Selvakumar G., Bhuvaneswari G., Thamizhiniyan P., Ravimycin T.

International Letters of Natural Sciences

|

2014

|

tom 08

|

nr 2

A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10-5 and 10-6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillus subtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.

2

Enhancement of vegetative parameters of brinjal in proplates by the application of bacterial endophytes- Azospirillum brasilense and Pseudomonas fluorescens

100%

Sivagamasundari U., Gandhi A.

International Letters of Natural Sciences

|

2014

|

tom 22

An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing ability was estimated. Based upon their N-fixing ability and IAA production, two strains, one Azospirillum sp. and one Pseudomonas sp. isolate was selected and tested for its performance in brinjal. The seeds treated with 75% Chemical fertilizer + Azospirillum brasilense + Pseudomonas fluorescens (T6) showed maximum plant vegetative characters, followed by others compared with control.

3

Pyoverdine production in Pseudomonas fluorescens UTPF5 and its association with suppression of common bean damping off caused by Rhizoctonia solani [Kuhn]

86%

Sharifi R., Ahmadzadeh M., Sharifi-Tehrani A., Talebi-Jahromi K.

Journal of Plant Protection Research

|

2010

|

tom 50

|

nr 1

72-78

Siderophore production is an important mechanism of biological control by a number of strains of plant growth-promoting rhizobacteria. Pseudomonas fluorescens UTPF5 was originally obtained from onion field. Biochemical and physiological characteristics of this strain refer to biovar 3 of P. fluorescens. Strain UTPF5 is an effective bacterium against several phytopathogenic fungi. Pyoverdine type siderophore of this strain was isolated using XAD amberlite column. The plant growth promotion and antifungal properties of bacteria were demonstrated under greenhouse conditions in combination with Fe-EDTA, Fe-EDDHA and Zn as modulators of pyoverdine production. Amendment with zinc, Fe-EDTA and Fe-EDDHA suppressed the disease inhibition when partially used with UTPF5. 7NSK2 and its pyoverdine mutant, MPFM1, were used as reference strains the inhibition percent of which was not affect by soil amendment. Iron chelates, especially Fe-EDDHA, increased growth and chlorophyll production by plants. This effect was improved in the presence of bacterial strains. The siderophore mutant MPFM1 did not exhibit satisfactory disease inhibition and growth promotion activity. In vitro experiments showed that purified pyoverdine could decrease the fungal growth to the same extent as pyoverdine-producing strain.

4

A comparison between Pseudomonas aureofaciens (chlororaphis) and P. fluorescens in biological control of cotton seedling damping-off disease

86%

Samavat S., Heydari A., Zamanizadeh H. R., Rezaee S., Aliabadi A. A.

Journal of Plant Protection Research

|

2014

|

tom 54

|

nr 2

Due to the importance of the biological control of plant diseases, testing and introducing new biocontrol-active microorganisms is a major concern among plant pathologists. The causal agent of cotton seedling damping-off disease is Rhizoctonia solani. In this regard, we tried to investigate the antagonistic activities of Pseudomonas aureofaciens (chlororaphis) 30–84 (phenazine producing wild type and non-phenazine producing mutant) strains on R. solani, in comparison with some isolates of P. fluorescent under both in vitro (laboratory) and in vivo (greenhouse) conditions. In the laboratory experiment, the inhibitory effects of all the bacteria, on the growth of R. solani, were evaluated using the dual culture procedure. Results showed that five isolates of P. fluorescent along with both strains of P. aureofaciens significantly inhibited the growth of R. solani. Effective bacterial antagonists were then evaluated in a greenhouse experiment where cotton seeds were coated with their suspensions and were sown in pasteurised field-soil. The soil had been pre-inoculated with a virulent isolate of R. solani. The efficacy of the bacterial antagonists was evaluated by counting the number of surviving seedlings in different treatments, at 15 and 60 days after sowing, for determining pre- and post-emergence damping-off incidence. According to the results of the greenhouse experiment, at both intervals, two isolates of P. fluorescens along with both strains of P. aureofaciens caused significant increases in the number of healthy seedlings, in comparison with the untreated control, and a commonly used fungicide (carboxin-thiram). The efficacy of phenazine producing a wild type strain of P. aureofaciens was higher than its non-phenazine producing mutant, indicating that phenazine plays an important role in the antagonistic activity of P. aureofaciens. Effective bacterial antagonists were then studied for their antagonistic mechanisms. The results showed that all four bacteria employed different mechanisms. The bacteria produced siderophore, and volatile metabolites and non-volatile metabolites, in their antagonistic activities. The results of this study suggest that P. auerofaciens may be a new biocontrol agent for controlling cotton seedling mortality disease.

5

Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils

86%

Abbas-Zadech P., Saleh-Rastin N., Asadi-Rahmani H., Khavazi K., Soltani A., Shoary-Nejati A. R., Miransari M.

Acta Physiologiae Plantarum

|

2010

|

tom 32

|

nr 2

Fluorescent pseudomonads are among the most influencing plant growth-promoting rhizobacteria in plants rhizosphere. In this research work the plant growth-promoting activities of 40 different strains of Pseudomonas fluorescens and Pseudomonas putida, previously isolated from the rhizosphere of wheat (Triticum aestivum L.) and canola (Brassica napus L.) and maintained in the microbial collection of Soil and Water Research Institute, Tehran, Iran, were evaluated. The ability of bacteria to produce auxin and siderophores and utilizing P sources with little solubility was determined. Four strains of Wp1 (P. putida), Cfp10 (Pseudomonas sp.), Wp150 (P. putida), and Wp159 (P. putida) were able to grow in the DF medium with ACC. Thirty percent of bacterial isolates from canola rhizosphere and 33% of bacterial isolates from wheat rhizosphere were able to produce HCN. The results indicate that most of the bacteria, tested in the experiment, have plant growth-promoting activities. This is the first time that such PGPR species are isolated from the Iranian soils. With respect to their great biological capacities they can be used for wheat and canola inoculation in different parts of the world, which is of very important agricultural implications.

6

Susceptibility of antagonistic microorganisms to some fungicides

86%

Machowicz-Stefaniak Z., Zalewska E., Krol E.

Annals of Agricultural Sciences. Series E: Plant Protection

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1998

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tom 27

|

nr 1-2

91-97

7

Influence of Fusarium oxysporum f. sp. cubense [E. F.Smith] Snyder and Hansen on 2,4-diacetylphloroglucinol production by Pseudomonas fluorescens migula in banana rhizosphere

86%

Saravanan T., Muthusamy M.

Journal of Plant Protection Research

|

2006

|

tom 46

|

nr 3

241-254

Influence of Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyder and Hansen on 2,4-diacetylphloroglucinol (DAPG) production in the rhizosphere of banana cultivar Rasthali by Pseudomonas fluorescens was investigated. The purified extracts of Pfm strain of P. fluorescens isolated from banana rhizosphere inhibited the growth and spore germination of F. oxysporum f. sp. cubense under laboratory conditions. DAPG extracted from the cultures of the strain was observed as distinct spots in thin layer chromatographic plates at Rf value of 0.88. The extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense eluted at retention time ranges from 20.00 min to 21.30 min. The quantity of DPAG production was less in the extracts of soil inoculated with P. fluorescens and challenge inoculated with F. oxysporum f. sp. cubense as compared to P. fluorescens alone inoculated soil. The talc formulation of Pfm strain also reduced vascular discolouration due to the pathogen in banana plants when inoculated at 15 g/plant.

8

Wpływ kwasu salicylowego syntetyzowanego przez bakterie Pseudomonas fluorescens i P. chlororaphis na fitopatogeniczne grzyby rodzaju Fusarium

86%

Jankiewicz U., Golab D., Frak M.

Polish Journal of Agronomy

|

2013

|

nr 15

Występujące powszechnie w ryzosferze bakterie z rodzaju Pseudomonas są zdolne do stymulacji wzrostu roślin. Mechanizm korzystnego oddziaływania tych bakterii na rośliny jest złożony, jednym z jego elementów jest ograniczanie wzrostu patogenów grzybowych roślin. Za antagonistyczne oddziaływanie bakterii Pseudomonas względem grzybów patogenicznych odpowiadają pozakomórkowe enzymy lityczne oraz metabolity wtórne. W pracy zbadano zdolność do syntezy kwasu salicylowego przez dwa szczepy bakterii z rodzaju Pseudomonas: P. fl uorescens (UM1) oraz P. chlororaphis (UM2). Bakteryjny kwas salicylowy wykrywano techniką chromatografi i cienkowarstwowej w układzie faz: kwas octowy:chloroform (9:1). Dodany do podłoża hodowlanego kwas salicylowy uzyskany po ekstrakcji octanem etylu z hodowli badanych bakterii spowodował znaczne zahamowanie wzrostu grzybni fi topatogenów z rodzaju Fusarium. Najmniej podatny na działanie kwasu salicylowego okazał się F. graminearum, natomiast największe zahamowanie wzrostu stwierdzono dla F. culmorum na podłożu z dodatkiem kwasu salicylowego z hodowli UM1. Wyniki przeprowadzonych doświadczeń wskazują na istotną rolę syntetyzowanego przez bakterie kwasu salicylowego w ograniczaniu wzrostu grzybowych patogenów z rodzaju Fusarium.

9

Survival of the cyanogenic strains of Pseudomonas fluorescens and their effect on indigenous microflora in the rhizosphere of bean [Phaseolus vulgaris L.]

86%

Piotrowska-Seget Z., Sroka K.

Acta Microbiologica Polonica

|

1996

|

tom 45

|

nr 1

10

Ocena wplywu kilku srodkow ochrony roslin na wybrane organizmy glebowe

81%

Klimach A., Wieczorek W.

Progress in Plant Protection

|

1998

|

tom 38

|

nr 2

587-589

11

Arbuscular mycorrhizal fungal-associated bacteria affect mycorrhizal colonization, essential oil and plant growth of Murraya koenigii L.

72%

Jangra E., Yadav K., Aggarwal A.

Acta Scientiarum Polonorum. Hortorum Cultus

|

2019

|

tom 18

|

nr 5

Murraya koenigii L. (family: Rutaceae), commonly referred to as curry leaf, is a highly valued plant due to its aroma and medicinal features. Two dominant AM species Glomus mosseae and Acaulospora laevis were isolated from the rhizospheric soil of M. koenigii. A pot experiment was performed to verify the interactive potential of G. mosseae and A. laevis alone or in combination with Pseudomonas fluorescens on M. koenigii. Various morphological and biochemical parameters were measured after 120 days. Overall results suggest that all co-inoculation treatments showed beneficial effects on all the growth, physiological and oil content. The overall results demonstrate that the co-inoculation of bioinoculants, like P. fluorescens with AM fungi, promotes higher AM colonization and spore number enhancing the nutrient acquisition, especially phosphorus (P), improving the rhizospheric condition of soil.

12

Wymienialnosc sideroforow przez bakterie rodzaju Pseudomonas

72%

Jankiewicz U., Kudelska J.

Woda - Środowisko - Obszary Wiejskie

|

2010

|

tom 10

|

nr 2[30]

93-102

13

Comparative sensitivity of Pseudomonas lachrymans (Sm. et B.) Carsner, P. fluorescens Migula and P. aeruginonsa (Schroeter) Migula to some antibiotics and other bactericidal compounds

72%

Jarosz J., Lipa J. J.

Prace Naukowe Instytutu Ochrony Roślin

|

1988

|

tom 30

|

nr 1-2

14

The effect of pesticides on Pseudomonas fluorescens count in water

72%

Zmyslowska I., Jankowska J.

Polish Journal of Natural Sciences

|

2005

|

tom 18

165-172

15

An attempt to protect winter wheat against Gaeumannomyces graminis var.tritici by the use of rhizobacteria Pseudomonas fluorescens and Bacillus mycoides

72%

Czaban J., Ksiezniak A., Wroblewska B., Paszkowski W. L.

Polish Journal of Microbiology

|

2004

|

tom 53

|

nr 2

Pseudomonas fluorescens strains III107 and II21 and Bacillus mycoides strains JC192 and K184, stimulating growth of winter wheat, were chosen for the studies. The bacterial strains inhibited on agar nutrient medium the growth of Gaeumannomyces graminis var. tritici (Ggt) - the pathogenic fungus causing take-all on wheat. Both strains of pseudomonads synthesized relatively high amounts of Fe³⁺ chelators. The strains of bacilli were characterized by the very fast spreading on agar media. Furthermore, strain II21 was highly cyanogenic, and strain JC192 highly chitinolytic. Bacterization of winter wheat seeds (especially with strains III107 and JC192) significantly reduced the percentage of the plants infested with the pathogen in the 28 day glasshouse pot experiment. In the plot experiment, the winter wheat seeds were inoculated with a mixture of strains III107, II21 and JC192. Due to the bacterization the yield of wheat grain and straw was higher in comparison to the series with Ggt alone by 122% and 75%, respectively, but it amounted only to 45% and 43% of the control series not contaminated with Ggt. The decrease of percentage of wheat ears with weight less than 500 mg from 61% in Ggt-series to 25% in Ggt-bacterized-series, and especially the decrease of percentage of wheat ears with weight less than 200 mg from 43% to 14% additionally indicate the partial protection of the winter wheat against Ggt by the rhizobacteria. In the experimental series not contaminated with Ggt the percentage of these wheat ears fractions did not exceed 3% and 0.5%, respectively.

16

Effects of introduced pseudomonads and soil treatment with copper on physiological state of indigenous microflora

72%

Kozdroj J., Piotrowska-Seget Z.

Acta Microbiologica Polonica

|

1996

|

tom 45

|

nr 1

17

Indigenous microflora responses to introduction of cyanogenic strains of Pseudomonas fluorescens into soil

72%

Piotrowska-Seget Z., Kozdroj J.

Acta Microbiologica Polonica

|

1999

|

tom 48

|

nr 1

18

Biological control of Verticillium wilt on hops

72%

Solarska E.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

2001

|

tom 49

|

nr 3

19

An attempt to protect winter wheat against Fusarium culmorum by the use of rhizobacteria Pseudomonas fluorescens and Bacillus mycoides

72%

Czaban J., Ksiezniak A., Perzynski A.

Polish Journal of Microbiology

|

2004

|

tom 53

|

nr 3

Inoculation of wheat seeds with two strains of Pseudomonas fluorescens (III107 and II21) and two strains of Bacillus mycoides (JC192 and K184) isolated from winter wheat roots, as well as with one strain of P. fluorescens (ID13) isolated from oat roots, reduced the negative influence of Fusarium culmorum on winter wheat in a 28 day pot experiment. The bacterial strains (especially III107 and chitinolytic JC192) markedly increased the plant seedlings emergence and the plant biomass (the shoots weight up to 252%, and the roots weight up to 229%) in comparison to the experimental series with F. culmorum alone. Also in a microplot experiment the yield of grain and straw of winter wheat, inoculated with the bacterial strains (especially JC192 and III107) and growing in soil contaminated with F. culmorum, was higher (the grain yield up to 120%, and the straw yield up to 139%) than in a series with F. culmorum alone (100%). In both experiments the highly cyanogenic strain II21 was least effective. A linear correlation (r = 0.926) and a rank Spearman's correlation (rSp= 0.991), both significant at p<0.01, between the weight of plant biomass in the pot experiment and the yield of whole shoots in the microplot experiment were found. It suggests that the same mechanisms worked in both experiments, although with different intensity.

20

The unique cyclopropane fatty acid composition of rhizospheral Pseudomonas fluorescent isolates

72%

Zarnowski R., Pietr S. J., Quilez J. F., Cabrera E., Barrero A. F.

Cellular and Molecular Biology Letters

|

2000

|

tom 05

|

nr 2

9,10-methylenehexadecanoic acid was found to be the main and sole constituent of the cyclopropane fatty acid (CFA) pool of five Pseudomonas fluorescent strains. Our findings have shown that all the studied isolates of plant-growth promoting rhizospheral fluorescent pseudomonads have qualitatively identical CFA profiles, even though they have the ability to make use of distinct carbon sources and show different plant root colonisation ability. This report suggests that the preservation of the proper CFA composition in biomembranes may be crucial for rhizobacteria adaptation to the fluctuation of physicochemical conditions in the rhizosphere.

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