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Evaluation of the prevalence and molecular characterization of Pneumocystis jirovecii in patients with a variety of respiratory diseases

100%

Sokulska M., Kicia M., Piesiak P., Matos O., Lobo M. L., Kopacz Z., Wesolowska M., Hendrich A. B.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

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Evaluation of the frequency of Pneumocystis jirovecii occurrence in a group of children hospitalized for acute respiratory infections

72%

Gołab E., Rozej-Bielicka W., Pancer K.

Wiadomości Parazytologiczne

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2011

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tom 57

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nr 2

Primary infection with Pneumocystis jirovecii in small children may cause inflammation of the respiratory tract which requires hospitalization. Lack of characteristic clinical symptoms makes it impossible to recognize P. jirovecii infections without performing laboratory analyses. Nasopharyngeal swabs from 70 children with respiratory tract infections were screened for fragments of the P. jirovecii genome. Pneumocystis DNA was found in swabs taken from two (2.9%) of the tested children: a newborn who was infected in the hospital and a six month old baby admitted to hospital two days after pneumonia was diagnosed. The obtained results confirm that primary P. jirovecii infections may occur in the form of acute respiratory tract inflammations suggesting a viral infection. In differential diagnosis of Pneumocystis infections in children molecular methods are useful as their high sensitivity makes it possible to analyze samples obtained in a non-invasive way.

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Ocena wystepowania zakazen Pneumocystis badaniem wymazow z gardla i jamy ustnej metoda nested PCR

58%

Golab E., Szkoda T., Dzbenski T. H.

Medycyna Doświadczalna i Mikrobiologia

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2006

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tom 58

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nr 4

371-376

Badano materiał uzyskany z wymazów z gardła i jamy ustnej metodą nested PCR w celu wykrycia infekcji Pneumocystis u ludzi i szczurów laboratoryjnych. Stwierdzono, że czułość nPCR umożliwia wykorzystanie wymazów do badania rozprzestrzenienia pneumocystozy wśród ludzi i zwierząt laboratoryjnych oraz stwarza możliwość bezpiecznej diagnostyki infekcji u pacjentów, których stan kliniczny uniemożliwia uzyskanie materiału z dolnych dróg oddechowych.

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The occurrence of Pneumocystis jirovecii in people from three different age groups of Warsaw [Poland] community

58%

Golab E., Sadkowska-Todys M., Szkoda M. T., Dzbenski T. H.

Acta Parasitologica

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2008

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tom 53

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nr 1

The three age groups of people from Warsaw: children, young adults and elderly people were examined for the prevalence of infection with Pneumocystis jirovecii. Nested PCR was used to amplify fragment of mitochondrial large subunit rRNA of the fungus in samples of oropharyngeal swabs. Nineteen (12.8%) of the 148 examined samples were positive for DNA of P. jirovecii. The samples collected from children were more often positive than the samples from young adults (p = 0.003) or from both groups of adults (p = 0.0029). Moreover, among adults (n = 99) proportion of infected women (n = 6; 12.5%) was significantly higher than men (n = 1; 2%). Results of the research confirm the high prevalence of Pneumocystis infection in children and indirectly point out to children as a possible source of infection for older people.

Ograniczanie wyników

1 Acta Parasitologica

1 Annals of Parasitology

1 Medycyna Doświadczalna i Mikrobiologia

1 Wiadomości Parazytologiczne

2 Dzbenski T. H.

2 Golab E.

1 Gołab E.

1 Hendrich A. B.

1 Kicia M.

1 Kopacz Z.

1 Lobo M. L.

1 Matos O.

1 Pancer K.

1 Piesiak P.

1 Rozej-Bielicka W.

1 Sadkowska-Todys M.

1 Sokulska M.

1 Szkoda M. T.

1 Szkoda T.

1 Wesolowska M.

1 2016

1 2011

1 2008

1 2006

Evaluation of the prevalence and molecular characterization of Pneumocystis jirovecii in patients with a variety of respiratory diseases

Evaluation of the frequency of Pneumocystis jirovecii occurrence in a group of children hospitalized for acute respiratory infections

Ocena wystepowania zakazen Pneumocystis badaniem wymazow z gardla i jamy ustnej metoda nested PCR

The occurrence of Pneumocystis jirovecii in people from three different age groups of Warsaw [Poland] community