Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 26

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  Microsporum canis
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Skin mycosis caused by Microsporum canis constitutes an increasing problem of epidemiological and epizootiological nature both in Europe and other non-european countries. Cats and dogs are the main reservoir of this cosmopolitan pathogen and the disease has often an enzootic course in many animal breedings. Because of lack any reports on this problem in Poland the epizootiological state is unknown and this fact grounded to undertake the studies. Of 48 pure-bred cats taking part in a display 22, i.e. 45.8 per cent proved to be carries of M. canis. The amount of spores was so high that in all cases the fungus was found by direct microscopic examinations. A much lower carrier-state was observed in Persian cats that did not take part in any display. Of 22 cats the presence of dermatophytes was noted in 4 animals (18.1 per cent), however only in two by direct microscopic examinations. By plating there were isolated M. canis from 2 cats, T. mentagrophytes from one animal and M. canis and T. mentagrophytes from one cat. In the group of short-hair cats of 15 animals only in two (13 per cent) there was stated M. canis. In contrast, in 99 dogs coming from individual owners and from one shelter for animals no carrier-state was found at all. The above examinations are the first report as regards the carrierstate of pathogenic dermatophytes in cats and dogs in Poland and the results are in general consistent with those published by other foreign authors. The findings point to the serious problem of microsporiosis in cats in Poland.
The aim of this study was an assessment of the immune response of cats experimentally infected with M. canis and a determination of the extent of immunity acquired after recovery. Studies were carried out on 24 domestic, short-haired cats, aged 3-4 months. The resistance of the animals was assessed by reinfection, and the degree of immune response. It has been found that after recovery from an experimental infection with M. canis, the cats which remained in an infected environment for 18 weeks showed no clinical mycotic changes, whereas control animals underwent natural infection within 6 weeks and their mycosis had a typical course. Furthermore reinfection with high doses of the fungus and skin scarification failed to break down this acquired immunity. Hypersensitivity of the delayed type, encountered in cats within 3 weeks after the experimental infection, persisted for 8 months of studies. In an analogous period of time cats infected naturally demonstrated an evidently weaker response determined by the skin test. Positive results of the migration inhibition test were obtained in the period of 3-6 weeks after infection, which corresponded with the period of the persistence of the clinical mycotic changes.
The studies were carried out on 71 long-hair cats (mostly Persians), nine short-hair cats (mixed breeds) and 21 dogs of different breeds suspected of ringworm. The samples of epidermis and hair from long-hair cats enabled the isolation of fungi from 80.3 per cent of the animals. Usually M. canis was found (95 per cent) while only sporadically Trichophyton mentagrophytes and Scopulariopsis sp. Out of 13 samples taken from ears, in 6 cases M. canis was isolated, and M. canis together with Malassezia pa- chydermatis in 4 cases. Out of 4 samples from paws M. canis was isolated in 3 cases. In the group of short-hair cats M. canis was noted in 22 per cent of the animals and also in 42.9 per cent of the dogs. Dermatophytosis was found mostly in cats aged 1-6 months (92.1 per cent) and dogs up to 2 years of age (71 per cent). Along with the increase in the age of the animals, the rate of infection decreased. In addition, M. canis was also isolated from 7 owners of cats and owners’ families in which local lesions on the skin were observed.
Investigations were carried out on 32 foxes aged approximately 2 months. The comparative studies on an experimental infection with Microsporum canis and Trichophyton spp revealed that in foxes infected with M. canis distinctive signs of the disease occurred at about 3 weeks after inoculation of the fungus. In silver foxes they were better expressed and lasted for 2—3 months and cleared after 3—4 months from infection. In polar foxes ringworm was of a lesser course: Clinical signs cleared within 2 months. In foxes infected with Trichophyton mentagrophytes var. granulosum the first clinical signs appeared as early as after 7 days, progressed to their maxima after 2—3 weeks and cleared at approximately 6 weeks. The administration of a vaccine Bovitrichovac II (inactivated T. verrucosum strain) proved to be ineffective in case of M. canis infection; in contrast it was effective in the treatment of foxes infected with T. mentagrophytes var. granulosum. Four different vaccines were used for prophylactic purposes: a) Bovitrichovac II, b) an inactivated combined vaccine containing T. verrucosum and T. mentagrophytes strains, c) two variants of inactivated vaccines comprising M. canis strains. Bovitrichovac II and the combined vaccine protected foxes from T. mentagrophytes var. granulosum infection but not from M. canis. In contrast, specific vaccines appeared to be of good immunogenicity as they protected the animals from infection with M. canis. The skin test performed on foxes vaccinated for prophylactic purpose with M. canis vaccines (both variants) showed a positive response after the administration of allergen made of a Trichophyton mentagrophytes strain. The results point to the presence of some common antigenic fractions among Microsporum and Trichophyton strains that are active and stimulate allergic reactions. However, they do not seem to play any role in the cross-immunity. The findings indicate that there are some prospects for elaborating specific immunoprophylaxis against M. canis infection of cats and dogs.
Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T. rubrum (CBS 120358), T. mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.
The purpose of the work was to determine the protective properties of a vaccine, called Felisvac Me, developed according to the authors’ own technology. Examinations were carried out on 33 domestic, shorthaired cats 1-6 months of age. The degree of specific immunity was evaluated by a challenge trial, contact infection (simulation of natural contagion), delayed type of hypersensitivity test and the migration inhibition test of leukocytes. It was found that the vaccine without an adjuvant in a dose of 2 ml did not fully protect one-month old cats from being infected with a virulent strain of M. canis. Cats, which were given a higher dose of the vaccine (3 ml per animal) adsorbed on aluminium hydroxide, turned out to be immune to both methods of infection by challenge trials. The vaccine elicited a delayed type of hypersensitivity that persisted on almost a similar level (0.8--1.1 mm) throughout the observation period, i.e. 5 months. Positive values (25.2-32.4%) of the leukocyte migration inhibition test - the consecutive determinant of cellular type of immunity, were found between 22 and 41 days after vaccination. The above vaccine is characterised by a high protective value; it is also safe for both animals and environment. No side effects have been observed.
Dermatophytosis in dogs is variable and can clinically mimics other skin diseases. Diagnosis based on clinical presentation may be misleading and additional laboratory tests are needed for a final diagnosis. A systematic diagnostic procedure can prevent a wrong diagnosis and allow for the right treatment.
Interactions between selected geophilic fungi (keratinofytic and keratinophylic species) and pathogenic dermatophytes were examined in dual cultures on Sabouraud glucose agar (SGA). It was demonstrated that Trichophyton mentagrophytes var. mentagrophytes together with T. mentagrophytes var., quinceanum are the species most sensitive to the influences of the geophilic fungi. Microsporum persicolor was found to be more resistant to these influences. Only a few geophilic species inhibited the growth of M. canis on SGA. Keratinophilic species, unable to initiate hair decomposition but utilize the products of this process, exerted the larger inhibitory effect on the pathogenic dermatophytes than keratinofytic fungi.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.