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Honeyberry’s popularity has been increasing because of its valuable traits: nutritional value of fruits (a high amount of ascorbic acid, potassium and polyphenolic compounds), early ripening (14 days before strawberry), exceptional hardiness and easy cultivation. However, there is still little known about the pollination biology of this species, which is essential for practical breeding purposes. In this study we evaluated pollen fertility in five cultivars and three breeding clones of this species. The impact of the manner of pollination on the fruit set and forming seeds has also been studied. The highest percentage of viable pollen was found in cultivars ‘Duet’ and ‘Wojtek’ (96%), whereas the pollen of ‘Chelyabinka’ cultivar had the lowest fertility (90%). The percentage of blue honeysuckle fruits obtained from open-pollinated flowers was high (on average 94.7%) compared to isolated flowers (on average 8.6%). The effect of cross-pollination with cv. ‘Duet’ indicates that the percentage of the fruit set, fruit weight and mass of produced seeds was higher when this cultivar was applied as maternal than paternal form. The number of seeds affected significantly the weight of the fruit in the case when cv. ’Duet’ was used as a paternal (correlation coefficient r = 0.45) or maternal form (r = 0.66), while for other methods of pollination this effect was insignificant. These results may be used to the most efficient establishment of plantations as well as for the realization of the breeding program of this valuable species.
The aim of 3 -year experiment was to evaluate quality and chemical composition of blue honeysuckle berries cultivar 'Czarna' and non-specified seedling "N" as de­pendent on time of ripening. The results obtained showed that berries harvested later were bigger but less firm. Furthermore, an increase of soluble solids content was observed in fruit of both cultigens at the end of harvest (for seedling "N" from 11 to 13.5% and for 'Czarna' from 9.8% to 12.6%). On the other hand, for late-harvested berries of both cultigens 20% decrease of titratable acidity and vitamin C was noticed. In berries of seedling "N" at the beginning of harvest 232 mg 100 g-1 of total polyphe­nols was determined, whereas the late ripening fruit contained 284 mg 100 g-1 of total polyphenols. Similar relation was observed in 'Czarna' berries (an increase from 164 mg 100 g-1 to 221 mg 100 g-1). The berries of late harvest contained more antho- cyanins (167 mg 100 g-1 in 'Czarna' and 227 mg 100 g-1 in seedling "N") as compared to early collected ones (122 and 173 mg 100 g-1 for, 'Czarna' and "N", respectively). Hydroxycinnamic acids content varied from 37.0 to 29.3 mg 100 g-1, respectively, for early and late collected berries of seedling "N" and from 22.3 mg 100 g-1 to 26.6 mg 100 g-1 for early and late harvested berries of 'Czarna'. Among flavonols, quercetin-3-rutinoside, quercetin-3-glucoside and two unidentified flavonols with retention times 26 min and 32 min, were determined. Luteolin 7-O-a-glucoside content varied from 4.59 to 7.05 mg 100 g-1 in the early and late harvested berries of seedling "N" and from 3.12 mg 100 g-1 in the early to 4.25 mg 100 g-1 in the late harvested berries of 'Czarna'.
Blue honeysuckle due to the high health benefits of its fruit, early ripening (before the first strawberries cultivars) and high frost resistance of both plants and flowers has gained the great popularity both in cultivation and breeding. The implementation of the breeding program of new cultivars requires the knowledge of the characteristics that determine its direction in the selected material. Therefore, in this study the evaluation of the phenological phases, yield, fruit weight, number of seeds per fruit and pollen viability in the cultivars and breeding clones of this species was made. The tested cultivars and clones had different levels of the analysed qualities except the pollen viability which was high but did not differ significantly within the genotypes. The evaluation of such characteristics as yield potential and fruit weight indicates that cultivars ‘Warszawa’, ‘Wojtek’ and T2 clone which had higher values of these characteristics as compared to other genotypes are possible to be used in breeding programme. Obtainment of the forms with early fruit ripening can be realised through the use of the selected Russian cultivars. The objective of this study was also to characterise the blue honeysuckle germplasms using RAPD markers and to assess their genetic similarity. The analysed primers produced 61 fragments out of which 57 (93.44%) were polymorphic. The genetic similarity matrix was made on the basis of RAPD markers. The mean genetic similarity was calculated at 0.56. The presented study confirms that the use of RAPD markers is a practical and effective method to evaluate the genetic similarity of blue honeysuckle genotypes and to establish genetic relationships between these genotypes.
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