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Phenolic acids of walnut (Juglans regia L.)

86%
The chromatographic separations showed the presence of eleven free phenolic acids (trans-cinnamic, o-coumaric, p-coumaric, chlorogenic, ferulic, gallic, caffeic, p-hydroxybenzoic, syringic, tannic and vanillic) in the walnut tissues. The p-hydroxybenzoic acid was a predominant compound in the green husks (618.8 µg·g-1), though, high content of it was found in leaves obtained in July (478.3 µg·g-1). The content of chlorogenic acid in green husks of walnut (306.7 µg·g-1) was twice higher than that in the leaves obtained in July. Similar level of coumaric acids was determined in the leaves and green husks (o-coumaric: 42.4–48.2 µg·g-1; p-coumaric: 78.6–94.3 µg·g-1), though, o-coumaric acid was not found in May-leaves. Moreover, leaves of walnut possessed high content of caffeic acid (leaves obtained in May – 456.9; leaves obtained in July – 262.1 µg·g-1) and ferulic acid (leaves obtained in May – 93.5; leaves obtained in July – 292.5 µg·g-1). The predominant compound of the male inflorescences was vanillic acid (359.5 µg·g-1). The green husks of walnut were rich in polyphenolic acids, syringic (427.0 µg·g-1) and tannic (237.4 µg·g-1). However, the content of tannic acid was higher in the leaves - especially in leaves collected in May (350.7 µg·g-1). In tested tissues of walnut, gallic acid was found in the lowest concentration (male inflorescences – 32.7 µg·g-1and leaves obtained in July – 12.7 µg·g-1). In general, there was a great variation in a level of the phenolic acids between the studied organs of the walnut; nevertheless, walnut tissues are precious source of phenolic acids.
The main aim of the study was to compare the radial growth of Persian walnut and Schrenk spruce trees growing under the same ecological conditions in the Sary-Chelek range of the Tien-Shan Mountains, as well as to analyse the response of these species to the selected climate factors in line with the altitude gradient. Four study plots were established at the altitude of 1350, 1400, 1450 and 1500 m a.s.l. Results indicated that (1) walnut and spruce in the Sary-Chelek Biosphere Reserve have different patterns of radial increment and reaction to climate factors, despite growing in the same habitat, (2) spruce radial growth responded to low precipitation and low temperature during the April to September period of the previous year; (3) walnut radial increment patterns varied significantly with changes in altitude, whereas spruce patterns did not; and (4) walnut radial increment patterns responded positively to high temperature during contemporary growing season and to precipitation during the prior growing season. In addition, it was noted that precipitation during the contemporary growing season could negatively influence growth.
Turkey is one of the most important walnut producers in the world. The aim of this study to select superior walnut genotypes among the walnut seedling populations naturally grown in Gumushacikoy district of Amasya province located in Inner Anatolia between 2010–2011 years. In the study, a large number walnut genotypes in Inner Anatolia were screened according to selection criteria and after evaluation twenty promising walnut genotypes were selected as cultivar candidate among genotypes. The average fruit weights, kernel weights and kernel ratios were ranged from 8.93 to 13.92 g, 4.62 to 7.36 g and 47.80 to 58.98% among twenty promising walnut genotypes, respectively. Measurements of fruit dimensions showed that the average fruit length, width and heights were found between 42.80–29.97 mm; 25.73–34.77 mm and 28.86–33.85 mm, respectively. Considering 20 promising walnut selections, 11, 5 and 4 genotypes had been found protandry, protogyny and homogamy. The chemical analyses showed that protein, crude oil and ash contents of selected twenty walnut genotypes were between 13.75–19.69%; 44.08– 70.81% and 1.53–2.15%, respectively.
A new model humic acid (HA) have been synthetized from 5-hydroxy-1,4-naphthoquinone (juglone, J), an allelopathic compound occuring in leaves, nut shells, exsudates of roots and barks of Juglans genera. Aqueous solutions of J were autooxidised at different pH 6-10 with mineral matrices: bentonite, glass and silica. The very slow autooxidation reaction was monitored by means of chemiluminescence (CL) imaging and kinetics supported by a highly sensitive CCD camera Molecular Light Imager. In the course of autooxidation that lasted three weeks, an extremely low-intensity of CL was detected The reaction liquid was further treated according to the IHSS protocol to isolate humic acid fractions. Elemental analysis, UV-VIS and FTIR absorption spectra as well as fluorescence excitation and emission spectra were measured to characterise this new HA.
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